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The map label for this gene is cysD

Identifier: 114799213

GI number: 114799213

Start: 788541

End: 789527

Strand: Reverse

Name: cysD

Synonym: HNE_0777

Alternate gene names: 114799213

Gene position: 789527-788541 (Counterclockwise)

Preceding gene: 114797227

Following gene: 114799988

Centisome position: 21.31

GC content: 62.51

Gene sequence:

>987_bases
ATGCGGACCCGACACGGAGCCTTTCTTCTGCTGGCAAGGCCCGCTACGGAACGCGCTCACACGCCAGAACAGGATCAGTA
CACCTTGCCCACCCTGACCCATCTTGACCGGCTGGAAGCCGAAAGCCTGCACATCCTCCGGGAAGTCGCGGCCGAATGCG
AAAAGCCGGTGATGCTGTATTCGATCGGCAAGGATTCGGCCGTGATGCTGCATCTGGCGATGAAGGCATTCTATCCGGCC
AGGCCGCCCTTCCCGCTGCTGCATGTCGATACCGGCTGGAAGTTCCGGGAGATGATCGCGTTCCGCGACCGCAAGGTTAA
AGAGCTGGGCCTGGAACTGCTCGTCCATATGAATGAGGACGGCGTGCGCGAAGGCGTTGGCCCCTTCACGCATGGCTCGG
CCTACCATACAGATGTGATGAAAACCGCCGCGCTGAAACAGGCGCTCGACAAGTACGGCTTTGACGCCGCCTTTGGCGGC
GCCCGGCGCGACGAAGAGAAGTCCCGCGCCAAGGAGCGCGTCATCTCCTTCCGCTCGGCCGGCCACCGCTGGGACCCCAA
ACGCCAGCGCCCGGAAATCTGGAACCTCTACAACACCCGGATGCACAAGGGCGAAAGCCTGCGCGCCTTCCCGCTGTCCA
ACTGGACCGAGCTCGACATCTGGCAGTACATTCGCCGCGAAGGCATCGAGCTCGTCCCGCTCTATCTGGCCGCGCCGCGC
CCCGTGGTCACCTGGAACGACACGCTGATCATGGTCGATGACGACCGCGTGCCCGCCGAGATCGCCGCGAAAGCGGAAGT
TAAATCCGTCCGTTTCCGGACGCTTGGCTGCTACCCGCTGACCGGCGCGGTGGAAAGCACCGCCACGACACTGGATGATG
TTATCCAGGAAATTCTCCTCACCACCACGTCCGAGCGCCAGGGCCGCGCCATCGACAAGGATCAGACCGCCTCCATGGAG
AAGAAGAAGCAGGAAGGCTATTTCTGA

Upstream 100 bases:

>100_bases
CAGGCTGCACAATGACGATTTATGTGCATTTGTGACACCAATTGCGGCGGATTCAGGTCTGGCGTCGGCTCGGCTTTCGT
TTAGCTTTACTGAAGGGTGA

Downstream 100 bases:

>100_bases
TGCGCCTCAACGACGATCTCATTGCTTCGGATATCGGCGCGTATCTGGAGGCGCACGAGAACAAATCGCTCCTGCGGTTC
ATCACCTGCGGCTCAGTCGA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRTRHGAFLLLARPATERAHTPEQDQYTLPTLTHLDRLEAESLHILREVAAECEKPVMLYSIGKDSAVMLHLAMKAFYPA
RPPFPLLHVDTGWKFREMIAFRDRKVKELGLELLVHMNEDGVREGVGPFTHGSAYHTDVMKTAALKQALDKYGFDAAFGG
ARRDEEKSRAKERVISFRSAGHRWDPKRQRPEIWNLYNTRMHKGESLRAFPLSNWTELDIWQYIRREGIELVPLYLAAPR
PVVTWNDTLIMVDDDRVPAEIAAKAEVKSVRFRTLGCYPLTGAVESTATTLDDVIQEILLTTTSERQGRAIDKDQTASME
KKKQEGYF

Sequences:

>Translated_328_residues
MRTRHGAFLLLARPATERAHTPEQDQYTLPTLTHLDRLEAESLHILREVAAECEKPVMLYSIGKDSAVMLHLAMKAFYPA
RPPFPLLHVDTGWKFREMIAFRDRKVKELGLELLVHMNEDGVREGVGPFTHGSAYHTDVMKTAALKQALDKYGFDAAFGG
ARRDEEKSRAKERVISFRSAGHRWDPKRQRPEIWNLYNTRMHKGESLRAFPLSNWTELDIWQYIRREGIELVPLYLAAPR
PVVTWNDTLIMVDDDRVPAEIAAKAEVKSVRFRTLGCYPLTGAVESTATTLDDVIQEILLTTTSERQGRAIDKDQTASME
KKKQEGYF
>Mature_328_residues
MRTRHGAFLLLARPATERAHTPEQDQYTLPTLTHLDRLEAESLHILREVAAECEKPVMLYSIGKDSAVMLHLAMKAFYPA
RPPFPLLHVDTGWKFREMIAFRDRKVKELGLELLVHMNEDGVREGVGPFTHGSAYHTDVMKTAALKQALDKYGFDAAFGG
ARRDEEKSRAKERVISFRSAGHRWDPKRQRPEIWNLYNTRMHKGESLRAFPLSNWTELDIWQYIRREGIELVPLYLAAPR
PVVTWNDTLIMVDDDRVPAEIAAKAEVKSVRFRTLGCYPLTGAVESTATTLDDVIQEILLTTTSERQGRAIDKDQTASME
KKKQEGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=300, Percent_Identity=69, Blast_Score=431, Evalue=1e-122,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_HYPNA (Q0C438)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759505.1
- ProteinModelPortal:   Q0C438
- SMR:   Q0C438
- STRING:   Q0C438
- GeneID:   4289582
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0777
- NMPDR:   fig|228405.5.peg.760
- TIGR:   HNE_0777
- eggNOG:   COG0175
- HOGENOM:   HBG758022
- OMA:   WQYIRRE
- PhylomeDB:   Q0C438
- ProtClustDB:   PRK05253
- BioCyc:   HNEP81032:HNE_0777-MONOMER
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 37535; Mature: 37535

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTRHGAFLLLARPATERAHTPEQDQYTLPTLTHLDRLEAESLHILREVAAECEKPVMLY
CCCCCCEEEEEECCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
SIGKDSAVMLHLAMKAFYPARPPFPLLHVDTGWKFREMIAFRDRKVKELGLELLVHMNED
ECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHEEECCCC
GVREGVGPFTHGSAYHTDVMKTAALKQALDKYGFDAAFGGARRDEEKSRAKERVISFRSA
CHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHC
GHRWDPKRQRPEIWNLYNTRMHKGESLRAFPLSNWTELDIWQYIRREGIELVPLYLAAPR
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCC
PVVTWNDTLIMVDDDRVPAEIAAKAEVKSVRFRTLGCYPLTGAVESTATTLDDVIQEILL
CEEEECCEEEEEECCCCCHHHHHHHHHHHHHEEEECCEECCCCHHHHHHHHHHHHHHHHH
TTTSERQGRAIDKDQTASMEKKKQEGYF
HCCCCCCCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MRTRHGAFLLLARPATERAHTPEQDQYTLPTLTHLDRLEAESLHILREVAAECEKPVMLY
CCCCCCEEEEEECCCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEE
SIGKDSAVMLHLAMKAFYPARPPFPLLHVDTGWKFREMIAFRDRKVKELGLELLVHMNED
ECCCCCHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHEEECCCC
GVREGVGPFTHGSAYHTDVMKTAALKQALDKYGFDAAFGGARRDEEKSRAKERVISFRSA
CHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHHC
GHRWDPKRQRPEIWNLYNTRMHKGESLRAFPLSNWTELDIWQYIRREGIELVPLYLAAPR
CCCCCCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCC
PVVTWNDTLIMVDDDRVPAEIAAKAEVKSVRFRTLGCYPLTGAVESTATTLDDVIQEILL
CEEEECCEEEEEECCCCCHHHHHHHHHHHHHEEEECCEECCCCHHHHHHHHHHHHHHHHH
TTTSERQGRAIDKDQTASMEKKKQEGYF
HCCCCCCCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA