| Definition | Hyphomonas neptunium ATCC 15444 chromosome, complete genome. |
|---|---|
| Accession | NC_008358 |
| Length | 3,705,021 |
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The map label for this gene is dnaG [H]
Identifier: 114799204
GI number: 114799204
Start: 3047761
End: 3049644
Strand: Reverse
Name: dnaG [H]
Synonym: HNE_2889
Alternate gene names: 114799204
Gene position: 3049644-3047761 (Counterclockwise)
Preceding gene: 114799941
Following gene: 114797662
Centisome position: 82.31
GC content: 64.76
Gene sequence:
>1884_bases ATGTCTTCTTCCCCCATTCGCATCCCTGATGGCTTCGTTGATGAGCTGAAGGCCCGTATCCGGCCTTCGGACGTGATCGG GCGCAAGGTGAAGCTGACGAAGAAGGGCAAGGAGTGGGTCGGCCTCTCGCCGTTCACGGCCGAGAAGACGCCGAGTTTCT ACGTCAACGACCAGAAGCGCATCTTCAAATGCTTTTCCTCCGGCATGGGCGGGGACGTGATCAACTTCGTGATGGAGACC GAGCGGCTCTCCTTCATGGAAGCGGTCGAGAAGCTGGCTGAGGAAGCGGGCATGGCCCTGCCCAAGGCGAGCCCGGCAGC GGCAGAGGAATATGATCACCGCAAGCGGTTGCAGGCGGCGTGCGAGGCGGCGGCGGATTTCTTCGAGGCGCGGCTGCGCT CCTCTGAAGGCGCGGGCGCGCGGACGTATCTGGAGGGCAGGGGGCTGGCGGCGTCGAGCTGGAGCAAATACCGCCTTGGC TACGCGCCCGATGACTGGCGGCGCACGCGGGCGCATCTGCAAGCCGAGGGCTTTACCGATGGCGAGCTGCTGGAAGCGGG CATCATCAAGGAAAACGACAAGGGCGGGGAGCCCTATGACGCGTTCCGCGGACGCCTGATGTTTCCGATCCCCGATTCGC GGGGCGGGATCATTGCGTTTGGCGGCCGCGCGCTGCAGCCCGATGCCAAGCCGAAATATCTCAATTCGGGCGACACGCCG CTCTTCCACAAATCAACCGTGCTCTATCGCTACAAGGCGGCGCGCGAGGCGCTGGGGCACGGCGAGGGCGGCGGGCTGAT CGTCTGCGAAGGCTATATGGACGTGATCGCGATGTGCGAGGCGGGCTTTGGCAATGCCGTTGCCCCGCTCGGCACGGCGC TGACCGAGGAGCAGTTGCAGCTGCTGTGGCGGGCCGGGCCGGAACCGGTAATGTGTTTTGATGGCGACAAGGCGGGGCTG GGCGCTGCGTATCGCGCGCTCGACCGGGCGCTGCCCTTTGCCGAGCCGGGGCGGTCTGTTTTCTTTGTGCTGCTGCCCGA CGGGATGGACCCGGACGACCTGATCCGCGCGCGCGGCGCCGGGGCGATGAGCGAGCAGCTGGCCGCTCGGTTGCCGCTGT CAGAGCTATTGTGGCGGCGCGAGCGGGACGCGGAGCCGCTGGACACGCCCGAGCGCCAGGCCGGGCTGGAAGCCCGCCTG ATCGCGGCATGCGGGCATATCCGGCATCCTGGTGTCAAATCCGCGTATGAGCGGGATTTGAAGTCGCGGCTGCGCGACCA TCTCTGGCAGCTGCGCGAAGCCAAACGGGCGGCCAATTACCAGAAGCGGCCAGGGCGGCCCGGCCGGCCCGGATCGGCAC AGATTCCCCCCGGCGGGGAAGAGGCGCAGGAACTTCTCAAGCAGGGCGGGCGCGAGAAGATTGGCGGGCTACGGCTGATC ATGAGCGCGGTCGATAATCCGGGTCTGTTATCGATCGGCGCGGAGTTGCTTGCCCAGTGTGCGCCGGCCGAACCGGATGC CGCGTTTCTGCGAGATGCAGTTCTTGACCTGGCAAATGACGGAATCCCCATTGACCGGGGGACAATCACCGCCCATCTAA CGAAATCTGGGAACATACGTGCTGCCGGTTTGCTCAAAGATTATCCCGTAACGCCTCAGATAGCGACCAATGGGTCCGAG GCACGGGAATGGCTAATCGCTCTGGAGCAATTTGTGGCGAAGCGGCGTTCCCGCGATCAGGAGACGGGCTCTGGTACGGA TAGTGCGAGCGCGGACCCTACGTTGTCTCCGCAAGAATGGCGGAGGCGGCTTCAAATGGTCGCGGAACGAAGGGCCCTCA AGGTCCGTATGAACGAGGCATCCAGCAAGAGCAGCGATAGCTGA
Upstream 100 bases:
>100_bases GGGGCCCTTTTTCGTGGACGGTGTAGTGGGCAATTGTATTTTTGGGGGAAATGCCAATCTCCGGTCCCGCAGCTCCGGCA AAGGTGGTATCCTGAAACCC
Downstream 100 bases:
>100_bases ACTGAACGGTAAAGCGACAGGCACTGGCACGGCAGCTTGCGAGGCTACCGGTCCGGACGGGAGATAGCTGAGATATGGCG ACAGCCACCAAGAAGAAAAA
Product: DNA primase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 627; Mature: 626
Protein sequence:
>627_residues MSSSPIRIPDGFVDELKARIRPSDVIGRKVKLTKKGKEWVGLSPFTAEKTPSFYVNDQKRIFKCFSSGMGGDVINFVMET ERLSFMEAVEKLAEEAGMALPKASPAAAEEYDHRKRLQAACEAAADFFEARLRSSEGAGARTYLEGRGLAASSWSKYRLG YAPDDWRRTRAHLQAEGFTDGELLEAGIIKENDKGGEPYDAFRGRLMFPIPDSRGGIIAFGGRALQPDAKPKYLNSGDTP LFHKSTVLYRYKAAREALGHGEGGGLIVCEGYMDVIAMCEAGFGNAVAPLGTALTEEQLQLLWRAGPEPVMCFDGDKAGL GAAYRALDRALPFAEPGRSVFFVLLPDGMDPDDLIRARGAGAMSEQLAARLPLSELLWRRERDAEPLDTPERQAGLEARL IAACGHIRHPGVKSAYERDLKSRLRDHLWQLREAKRAANYQKRPGRPGRPGSAQIPPGGEEAQELLKQGGREKIGGLRLI MSAVDNPGLLSIGAELLAQCAPAEPDAAFLRDAVLDLANDGIPIDRGTITAHLTKSGNIRAAGLLKDYPVTPQIATNGSE AREWLIALEQFVAKRRSRDQETGSGTDSASADPTLSPQEWRRRLQMVAERRALKVRMNEASSKSSDS
Sequences:
>Translated_627_residues MSSSPIRIPDGFVDELKARIRPSDVIGRKVKLTKKGKEWVGLSPFTAEKTPSFYVNDQKRIFKCFSSGMGGDVINFVMET ERLSFMEAVEKLAEEAGMALPKASPAAAEEYDHRKRLQAACEAAADFFEARLRSSEGAGARTYLEGRGLAASSWSKYRLG YAPDDWRRTRAHLQAEGFTDGELLEAGIIKENDKGGEPYDAFRGRLMFPIPDSRGGIIAFGGRALQPDAKPKYLNSGDTP LFHKSTVLYRYKAAREALGHGEGGGLIVCEGYMDVIAMCEAGFGNAVAPLGTALTEEQLQLLWRAGPEPVMCFDGDKAGL GAAYRALDRALPFAEPGRSVFFVLLPDGMDPDDLIRARGAGAMSEQLAARLPLSELLWRRERDAEPLDTPERQAGLEARL IAACGHIRHPGVKSAYERDLKSRLRDHLWQLREAKRAANYQKRPGRPGRPGSAQIPPGGEEAQELLKQGGREKIGGLRLI MSAVDNPGLLSIGAELLAQCAPAEPDAAFLRDAVLDLANDGIPIDRGTITAHLTKSGNIRAAGLLKDYPVTPQIATNGSE AREWLIALEQFVAKRRSRDQETGSGTDSASADPTLSPQEWRRRLQMVAERRALKVRMNEASSKSSDS >Mature_626_residues SSSPIRIPDGFVDELKARIRPSDVIGRKVKLTKKGKEWVGLSPFTAEKTPSFYVNDQKRIFKCFSSGMGGDVINFVMETE RLSFMEAVEKLAEEAGMALPKASPAAAEEYDHRKRLQAACEAAADFFEARLRSSEGAGARTYLEGRGLAASSWSKYRLGY APDDWRRTRAHLQAEGFTDGELLEAGIIKENDKGGEPYDAFRGRLMFPIPDSRGGIIAFGGRALQPDAKPKYLNSGDTPL FHKSTVLYRYKAAREALGHGEGGGLIVCEGYMDVIAMCEAGFGNAVAPLGTALTEEQLQLLWRAGPEPVMCFDGDKAGLG AAYRALDRALPFAEPGRSVFFVLLPDGMDPDDLIRARGAGAMSEQLAARLPLSELLWRRERDAEPLDTPERQAGLEARLI AACGHIRHPGVKSAYERDLKSRLRDHLWQLREAKRAANYQKRPGRPGRPGSAQIPPGGEEAQELLKQGGREKIGGLRLIM SAVDNPGLLSIGAELLAQCAPAEPDAAFLRDAVLDLANDGIPIDRGTITAHLTKSGNIRAAGLLKDYPVTPQIATNGSEA REWLIALEQFVAKRRSRDQETGSGTDSASADPTLSPQEWRRRLQMVAERRALKVRMNEASSKSSDS
Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]
COG id: COG0358
COG function: function code L; DNA primase (bacterial type)
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 Toprim domain [H]
Homologues:
Organism=Escherichia coli, GI1789447, Length=421, Percent_Identity=37.7672209026128, Blast_Score=287, Evalue=2e-78,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR016136 - InterPro: IPR013264 - InterPro: IPR019475 - InterPro: IPR006295 - InterPro: IPR013173 - InterPro: IPR006171 - InterPro: IPR002694 [H]
Pfam domain/function: PF10410 DnaB_bind; PF08278 DnaG_DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]
EC number: 2.7.7.-
Molecular weight: Translated: 68458; Mature: 68327
Theoretical pI: Translated: 8.05; Mature: 8.05
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
1.1 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 3.3 %Cys+Met (Translated Protein) 1.1 %Cys (Mature Protein) 2.1 %Met (Mature Protein) 3.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSSSPIRIPDGFVDELKARIRPSDVIGRKVKLTKKGKEWVGLSPFTAEKTPSFYVNDQKR CCCCCCCCCCCHHHHHHHHCCCHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCHHH IFKCFSSGMGGDVINFVMETERLSFMEAVEKLAEEAGMALPKASPAAAEEYDHRKRLQAA HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH CEAAADFFEARLRSSEGAGARTYLEGRGLAASSWSKYRLGYAPDDWRRTRAHLQAEGFTD HHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCC GELLEAGIIKENDKGGEPYDAFRGRLMFPIPDSRGGIIAFGGRALQPDAKPKYLNSGDTP HHHHHHCCEECCCCCCCCHHHHCCEEEEECCCCCCCEEEECCEEECCCCCCCCCCCCCCC LFHKSTVLYRYKAAREALGHGEGGGLIVCEGYMDVIAMCEAGFGNAVAPLGTALTEEQLQ CEECCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH LLWRAGPEPVMCFDGDKAGLGAAYRALDRALPFAEPGRSVFFVLLPDGMDPDDLIRARGA HHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCC GAMSEQLAARLPLSELLWRRERDAEPLDTPERQAGLEARLIAACGHIRHPGVKSAYERDL CHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHCCCCCCCHHHHHHHHH KSRLRDHLWQLREAKRAANYQKRPGRPGRPGSAQIPPGGEEAQELLKQGGREKIGGLRLI HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHH MSAVDNPGLLSIGAELLAQCAPAEPDAAFLRDAVLDLANDGIPIDRGTITAHLTKSGNIR HHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEE AAGLLKDYPVTPQIATNGSEAREWLIALEQFVAKRRSRDQETGSGTDSASADPTLSPQEW EEECCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH RRRLQMVAERRALKVRMNEASSKSSDS HHHHHHHHHHHHHHEEECCCCCCCCCC >Mature Secondary Structure SSSPIRIPDGFVDELKARIRPSDVIGRKVKLTKKGKEWVGLSPFTAEKTPSFYVNDQKR CCCCCCCCCCHHHHHHHHCCCHHHCCCEEEEECCCCCCCCCCCCCCCCCCCEEECCHHH IFKCFSSGMGGDVINFVMETERLSFMEAVEKLAEEAGMALPKASPAAAEEYDHRKRLQAA HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH CEAAADFFEARLRSSEGAGARTYLEGRGLAASSWSKYRLGYAPDDWRRTRAHLQAEGFTD HHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCEECCCCCCHHHHHHHHHHCCCCCC GELLEAGIIKENDKGGEPYDAFRGRLMFPIPDSRGGIIAFGGRALQPDAKPKYLNSGDTP HHHHHHCCEECCCCCCCCHHHHCCEEEEECCCCCCCEEEECCEEECCCCCCCCCCCCCCC LFHKSTVLYRYKAAREALGHGEGGGLIVCEGYMDVIAMCEAGFGNAVAPLGTALTEEQLQ CEECCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH LLWRAGPEPVMCFDGDKAGLGAAYRALDRALPFAEPGRSVFFVLLPDGMDPDDLIRARGA HHHHCCCCCEEEECCCCCCHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCC GAMSEQLAARLPLSELLWRRERDAEPLDTPERQAGLEARLIAACGHIRHPGVKSAYERDL CHHHHHHHHHCCHHHHHHHHHCCCCCCCCCHHHHCCHHHHHHHHCCCCCCCHHHHHHHHH KSRLRDHLWQLREAKRAANYQKRPGRPGRPGSAQIPPGGEEAQELLKQGGREKIGGLRLI HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHH MSAVDNPGLLSIGAELLAQCAPAEPDAAFLRDAVLDLANDGIPIDRGTITAHLTKSGNIR HHHCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEE AAGLLKDYPVTPQIATNGSEAREWLIALEQFVAKRRSRDQETGSGTDSASADPTLSPQEW EEECCCCCCCCCCEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHH RRRLQMVAERRALKVRMNEASSKSSDS HHHHHHHHHHHHHHEEECCCCCCCCCC
PDB accession: NA
Resolution: NA
Structure class: Unstructured
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 10761919 [H]