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The map label for this gene is cobQ

Identifier: 114799121

GI number: 114799121

Start: 2255386

End: 2256873

Strand: Reverse

Name: cobQ

Synonym: HNE_2169

Alternate gene names: 114799121

Gene position: 2256873-2255386 (Counterclockwise)

Preceding gene: 114797365

Following gene: 114799733

Centisome position: 60.91

GC content: 66.47

Gene sequence:

>1488_bases
ATGACCGCTCGCGCTATCATGATCCAGGGCACGGGCTCCGATGTCGGCAAATCGCTCCTCGTCGCGGGGTTCTGCCGCCT
TGCTGCGCGGCGCGGGCTTTCCGTTGCGCCGTTCAAGCCGCAGAACATGTCCAACAATGCTGCTGCAACGGCGGATGGTG
GCGAGATCGGCCGCGCCCAGGCGCTCCAGGCCCGCGCCTGCGGCCTTGATCCGCAGACGGATTTCAATCCCGTCCTGCTC
AAGCCCCAGTCAGACTGCACGGCGCAGGTCATCGTTCACGGCCGCCCGACCGCGACGCTTGAGGCGGCAGACTATATGGC
CCGCCGGGACACTCTGCGCGGCGCGGTCCTTGAAAGTTTCGCCCGCCTCACTGCCCGGTTTGATCTGGTCATCGTCGAAG
GTGCCGGCAGCCCGGCCGAAATCAATCTCCGCGACCGCGACATTGCCAATATGGGGTTTGCCCGCGCGGCCGGTGTGCCT
GTGGTTCTGGCCGGCGACATTGACCGCGGCGGGGTCATCGCCGCGCTCGTTGGCACCAAAACGGTCATCGATCCTGCCGA
CGCTGCCATGATCCGGGCCTTCCTCATCAACAAGTTCCGGGGCGATCCCGCTCTGTTTGAGCCCGCGATGGCAGACATCG
AGCGGATGACAGGTTGGGCCGGCCTCGGCATTGTCCCCTGGCTCAGCGCCGCTGCGCGGCTGCCTGCCGAGGATGGCGTA
GCCCTGGAGCAGATGCGGCCCACCGGTGGCGGCAGGATACGGATCGCCGCGCCCATGCTGTCACGCATCGCCAACTTTGA
CGACGCAGATCCTCTCCGCGCCGAGCCGAGTGTGGATTTCTTCTTCGTCCCGCCGGGGCAGGCGATCCCGCGCGATGTGG
ATGTGATCCTCCTGTTCGGCACAAAGTCGACCCTAGGAGATATGGCGTTCCTCCGCGCGCAAGGCTGGGATCATGACATC
CTCGCCCATGCGCGCACGGGCGGGCGGATCCTTGGCATCTGCGGCGGCTATCAGATGCTGGGCCAGTGGCTCTGCGATCC
TGAAGGGGTGGATGGCGCGGCCGGTGAACTGCCTGGCCTCGGCCTTCTGAAAACCGATACCACCATGCAGGGGGAGAAGA
CCGTTCGCCCCGTTACCGGACAGTGTGCCCGCACCGGCCTTCCCGTTTCGGGCTACGAAATCCATGCCGGCCTCACGCGG
GGGCCAGACGCTGCCCGTCCGTTCCTGCATCTTCCCGAAGGGCCGGATGGAGCCATCAGCCCGGACGGGCGCGTTGAAGG
CACATACGTGCATGGCCTATTCGCTCAAGACGCCTTCCGCGCCGCCTGGCTGGAAAACGTGCGGGCAGGGGCCTCGTCAG
ACGCCGCCTATGGTGCCTCGGTGGAGGCCGCTCTGGATGAACTGGCAGACGGTCTTGAGGCGCATCTTGATGTGGATCGC
TTCTTCGCCCTCGCGGCGGCGCCCGGCTGGCGGGGCGCTCCATCCTGA

Upstream 100 bases:

>100_bases
TGATCATGCCGCGATTGACGGCCCTGCGCCGCTTGGCGGCTAGGATTGATGCCGCGTGCGCTAGCAGTTGCATCACGCAA
TCCCCCAAGGAACACCTCAA

Downstream 100 bases:

>100_bases
CCTCCAGGGGGAACCATGAGAGGCCCAGGGCGGGATCAGTGCTGACCGGATTTGTCCAGATTTGTCCGGATTTGTCCTGG
TTGTGACACGTGCAGTCCCG

Product: cobyric acid synthase CobQ

Products: ADP; phosphate; adenosylcobyric acid; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 495; Mature: 494

Protein sequence:

>495_residues
MTARAIMIQGTGSDVGKSLLVAGFCRLAARRGLSVAPFKPQNMSNNAAATADGGEIGRAQALQARACGLDPQTDFNPVLL
KPQSDCTAQVIVHGRPTATLEAADYMARRDTLRGAVLESFARLTARFDLVIVEGAGSPAEINLRDRDIANMGFARAAGVP
VVLAGDIDRGGVIAALVGTKTVIDPADAAMIRAFLINKFRGDPALFEPAMADIERMTGWAGLGIVPWLSAAARLPAEDGV
ALEQMRPTGGGRIRIAAPMLSRIANFDDADPLRAEPSVDFFFVPPGQAIPRDVDVILLFGTKSTLGDMAFLRAQGWDHDI
LAHARTGGRILGICGGYQMLGQWLCDPEGVDGAAGELPGLGLLKTDTTMQGEKTVRPVTGQCARTGLPVSGYEIHAGLTR
GPDAARPFLHLPEGPDGAISPDGRVEGTYVHGLFAQDAFRAAWLENVRAGASSDAAYGASVEAALDELADGLEAHLDVDR
FFALAAAPGWRGAPS

Sequences:

>Translated_495_residues
MTARAIMIQGTGSDVGKSLLVAGFCRLAARRGLSVAPFKPQNMSNNAAATADGGEIGRAQALQARACGLDPQTDFNPVLL
KPQSDCTAQVIVHGRPTATLEAADYMARRDTLRGAVLESFARLTARFDLVIVEGAGSPAEINLRDRDIANMGFARAAGVP
VVLAGDIDRGGVIAALVGTKTVIDPADAAMIRAFLINKFRGDPALFEPAMADIERMTGWAGLGIVPWLSAAARLPAEDGV
ALEQMRPTGGGRIRIAAPMLSRIANFDDADPLRAEPSVDFFFVPPGQAIPRDVDVILLFGTKSTLGDMAFLRAQGWDHDI
LAHARTGGRILGICGGYQMLGQWLCDPEGVDGAAGELPGLGLLKTDTTMQGEKTVRPVTGQCARTGLPVSGYEIHAGLTR
GPDAARPFLHLPEGPDGAISPDGRVEGTYVHGLFAQDAFRAAWLENVRAGASSDAAYGASVEAALDELADGLEAHLDVDR
FFALAAAPGWRGAPS
>Mature_494_residues
TARAIMIQGTGSDVGKSLLVAGFCRLAARRGLSVAPFKPQNMSNNAAATADGGEIGRAQALQARACGLDPQTDFNPVLLK
PQSDCTAQVIVHGRPTATLEAADYMARRDTLRGAVLESFARLTARFDLVIVEGAGSPAEINLRDRDIANMGFARAAGVPV
VLAGDIDRGGVIAALVGTKTVIDPADAAMIRAFLINKFRGDPALFEPAMADIERMTGWAGLGIVPWLSAAARLPAEDGVA
LEQMRPTGGGRIRIAAPMLSRIANFDDADPLRAEPSVDFFFVPPGQAIPRDVDVILLFGTKSTLGDMAFLRAQGWDHDIL
AHARTGGRILGICGGYQMLGQWLCDPEGVDGAAGELPGLGLLKTDTTMQGEKTVRPVTGQCARTGLPVSGYEIHAGLTRG
PDAARPFLHLPEGPDGAISPDGRVEGTYVHGLFAQDAFRAAWLENVRAGASSDAAYGASVEAALDELADGLEAHLDVDRF
FALAAAPGWRGAPS

Specific function: Catalyzes amidations at positions B, D, E, and G on adenosylcobyrinic A,C-diamide. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation

COG id: COG1492

COG function: function code H; Cobyric acid synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COBQ_HYPNA (Q0C077)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760866.1
- ProteinModelPortal:   Q0C077
- STRING:   Q0C077
- GeneID:   4289916
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2169
- NMPDR:   fig|228405.5.peg.2090
- TIGR:   HNE_2169
- eggNOG:   COG1492
- HOGENOM:   HBG302271
- OMA:   AHTDLNP
- BioCyc:   HNEP81032:HNE_2169-MONOMER
- HAMAP:   MF_00028
- InterPro:   IPR002586
- InterPro:   IPR017929
- InterPro:   IPR004459
- InterPro:   IPR011698
- TIGRFAMs:   TIGR00313

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3

EC number: 6.3.5.10

Molecular weight: Translated: 51895; Mature: 51763

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS51274 GATASE_COBBQ

Important sites: ACT_SITE 334-334 ACT_SITE 431-431

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTARAIMIQGTGSDVGKSLLVAGFCRLAARRGLSVAPFKPQNMSNNAAATADGGEIGRAQ
CCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCHHH
ALQARACGLDPQTDFNPVLLKPQSDCTAQVIVHGRPTATLEAADYMARRDTLRGAVLESF
HHHHHHCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
ARLTARFDLVIVEGAGSPAEINLRDRDIANMGFARAAGVPVVLAGDIDRGGVIAALVGTK
HHHHEEEEEEEEECCCCCCEEEECCCCHHHCCCHHHCCCCEEEECCCCCCCEEEEEECCC
TVIDPADAAMIRAFLINKFRGDPALFEPAMADIERMTGWAGLGIVPWLSAAARLPAEDGV
EEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC
ALEQMRPTGGGRIRIAAPMLSRIANFDDADPLRAEPSVDFFFVPPGQAIPRDVDVILLFG
CHHHCCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEE
TKSTLGDMAFLRAQGWDHDILAHARTGGRILGICGGYQMLGQWLCDPEGVDGAAGELPGL
CCCCHHHHHHHHHCCCCHHHHEEECCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCC
GLLKTDTTMQGEKTVRPVTGQCARTGLPVSGYEIHAGLTRGPDAARPFLHLPEGPDGAIS
EEEECCCCCCCCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCCCCCEECCCCCCCCCC
PDGRVEGTYVHGLFAQDAFRAAWLENVRAGASSDAAYGASVEAALDELADGLEAHLDVDR
CCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHH
FFALAAAPGWRGAPS
HHHHHCCCCCCCCCC
>Mature Secondary Structure 
TARAIMIQGTGSDVGKSLLVAGFCRLAARRGLSVAPFKPQNMSNNAAATADGGEIGRAQ
CCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCCCCCCCCCHHH
ALQARACGLDPQTDFNPVLLKPQSDCTAQVIVHGRPTATLEAADYMARRDTLRGAVLESF
HHHHHHCCCCCCCCCCCEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHH
ARLTARFDLVIVEGAGSPAEINLRDRDIANMGFARAAGVPVVLAGDIDRGGVIAALVGTK
HHHHEEEEEEEEECCCCCCEEEECCCCHHHCCCHHHCCCCEEEECCCCCCCEEEEEECCC
TVIDPADAAMIRAFLINKFRGDPALFEPAMADIERMTGWAGLGIVPWLSAAARLPAEDGV
EEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCC
ALEQMRPTGGGRIRIAAPMLSRIANFDDADPLRAEPSVDFFFVPPGQAIPRDVDVILLFG
CHHHCCCCCCCEEEEEHHHHHHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCEEEEEEE
TKSTLGDMAFLRAQGWDHDILAHARTGGRILGICGGYQMLGQWLCDPEGVDGAAGELPGL
CCCCHHHHHHHHHCCCCHHHHEEECCCCEEEEECCCHHHHHHHCCCCCCCCCCCCCCCCC
GLLKTDTTMQGEKTVRPVTGQCARTGLPVSGYEIHAGLTRGPDAARPFLHLPEGPDGAIS
EEEECCCCCCCCCCCCCCCCHHHHCCCCCCCEEEECCCCCCCCCCCCCEECCCCCCCCCC
PDGRVEGTYVHGLFAQDAFRAAWLENVRAGASSDAAYGASVEAALDELADGLEAHLDVDR
CCCCEEEEEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCHHH
FFALAAAPGWRGAPS
HHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyrinic acid a,c-diamide; L-glutamine; H2O

Specific reaction: 4 ATP + adenosylcobyrinic acid a,c-diamide + 4 L-glutamine + 4 H2O = 4 ADP + 4 phosphate + adenosylcobyric acid + 4 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA