Click here to switch to the map view.

The map label for this gene is metH [H]

Identifier: 114799087

GI number: 114799087

Start: 2174913

End: 2177555

Strand: Reverse

Name: metH [H]

Synonym: HNE_2100

Alternate gene names: 114799087

Gene position: 2177555-2174913 (Counterclockwise)

Preceding gene: 114800042

Following gene: 114800553

Centisome position: 58.77

GC content: 60.42

Gene sequence:

>2643_bases
ATGACAGACGCAACAGCTACATTCATCAATATCGGTGAACGCACCAACATCACCGGCTCTGCCGTGTTCCGGAAGATGAT
TACCGACGGGCGCTACGGCGATGCCGTGGAGGTCGCCCGCCAACAGGTGGAGAGCGGCGCGCAGGTGATCGATGTAAACA
TGGATGAAGGCATGCTGGACGGCGTGCAGGCCATGACCACCTTCCTGAACCTGATTGCCGCCGAGCCAGATATTGCGCGC
GTGCCGGTTATGATCGACTCTTCCAAATGGGAAGTGATCGAGGCGGGCCTGAAATGCGTTCAGGGCAAAGCCATCGTCAA
CTCGATCTCCATGAAGGAAGGCGAGGACAAGTTCCGCGAGCAGGCGCGGGCGTGTCTGTCCTATGGCGCAGCCGTCGTCG
TGATGGCGTTTGATGAACAGGGGCAGGCCGATACCGCGCCGCGCAAGATCGAGATTTGCCAGCGGGCGTTCCGTATCCTG
ACCGAAGAAGTTGGATTCCCGGCTGAAGACATCATCTTCGATCCCAACGTTTTCGCTGTGGCGACGGGGATTGAGGAGCA
CAATACCTACGCGCTGGACTTTATCGAAGCGACGCGGGAAATCCGCAGGACTTGCCCTGGCTGCCACATCTCTGGCGGGC
TTTCCAACGTGTCTTTCTCCTTCCGGGGCAACGAACCGGTGCGCCGGGCGATGCACTCTGTGTTCCTGTATTATGCGATC
CCTGCGGGCATGGACATGGGCATCGTGAATGCCGGGCAGCTGGATATATATGACGACATTGACCGGGAATTGCGCGAGGC
CGTGGAGGATGTGATCCTCAACCGCCGCGCTGATGCGACCGACCGGCTTGTGGACCTGGCCGAACGGTTCAAGGGACAAA
AAGGCAAAGTGCAGGTCAAGGATCTGGCCTGGCGGGAGCTGCCCGTTGCCAAGCGGCTGGAGCATGCGCTGGTGCATGGC
ATCACCGAGTTTATTGATACGGACACGGAAGAAGCGCGCCTGAGCGTGGCGCGGCCGCTGCATGTGATTGAAGGGCCGCT
GATGGCGGGCATGAATGTGGTGGGCGACCTGTTCGGGTCGGGCAAGATGTTCCTGCCGCAGGTGGTAAAATCAGCCCGCG
TGATGAAGCAGGCTGTGGCCTGGCTTGAGCCCTACATGGAGGCGGAAAAGCGGGAGCTGGGTCTGGAGCGCAAATCGAAT
GGCAAGGTGATCCTAGCCACGGTGAAGGGCGATGTGCATGACATCGGTAAGAACATTGTTGGCGTGGTTCTGGCCTGTAA
CGGGTACGAGATTGTCGATCTCGGCGTGATGGTGCCGGCGGAAAAAATCCTCGATACGGCGGTGCGCGAGAAGGCCGACA
TTATCGGGCTTTCCGGGCTGATCACGCCGAGCCTTGATGAGATGGTCTATGTTGCGTCCGAGATGCAGCGCCGGGGCATG
GTGCAGCCGCTGCTGATTGGCGGGGCAACGACGAGCGCAGCGCATACGGCGGTGAAGATCGAGCCCGCGATCCGCTCCGC
GCCGGTGGTGCATGTGGTGGATGCCAGCCGCGCGGTCGGCGTCGTCAGTACACTGCTCAGCGATGAGGATCGCCCGGTAT
TTCTGGGCGAAGTACGCGCGCGGTATGAGCGGATGCGGGAAGCCCGCAAGGATGGACCGCCCAAGCCGCGCCTGCCTATT
GCCGAAGCGCGCGCTGCCAAGCTCAAATTCGACTGGGACAGCTATACACCGCCGAGGCCCACCTATGAGGGTGTGCGGAC
GTTTGAGGACATCGATCTGGGCACGCTGGCCCGCTATATCGACTGGACGCCGTATTTCTCTGCCTGGGATCTGGCGGGGC
GCTATCCGGCCATTCTGGAAGACAAGATCGTGGGCGAGGCAGCCTCCAGCCTGTGGCGGGATACCCAGGCGATGATGCAG
CAGCTGGTCGGGGAAGACTGGCTGAAGCCGAAAGCGGTTGTAGGTTTCTGGAAAGCCAATTCCGCTGGGGATGATGTCAA
ACTTGCCAATGGCGAGGCCTTCCTGACTCTGCGCCAGCAGATGATGAAGGATAACAGCCAGCCAAACTTTGCGCTGGCTG
ATTTCGTCGCTCCAAAGGGGGATGATTGGATTGGCGGCTTCTGCGTCACCTCTGGTCCGGAAGTTGAAGACATCGCGCAG
AGATTTGCCGCCAAGGGCGACGATTATTCTTCCATCATGGTGAAAGCACTGGCCGACCGGTTTGCCGAGGCCATGGCCGA
GTACATGCACGAAAAGGTCCGCAAGGAACTCTGGGCCTATGCGCCGGACGAAGCGCTTTCTACGGACGAACTGATCGCGG
AGAAATATCAGGGTATCCGCCCGGCGGCGGGCTATCCCTGCCAGCCAGACCATACCGAGAAAGCGACGCTGTTCCGCCTG
CTGGAAGCAGAGAAACGTATCGGCGTGGAGCTGACCTCCAGCTATGCGATGATGCCCGCGTCCAGCGTGTCGGGGCTGTA
TCTTTCGCACCCGAAGAGCGTCTACTTTGCCGTCGGCCGGATCGGCCGGGATCAGGTGGCTGACTATGCCGCGCGCAAGG
GCTGGGAAACGCGCATGGCCGAACGCTGGCTGGCGCCGATCCTCTCATATGATCCATTCGTGATGGCGGATGCAGTAGCC
TAG

Upstream 100 bases:

>100_bases
CGGGCGAGCAGGGCTGGATTTATATCTACAATCAGAAGACGGACGGCTTTGCCATTCTCCCGGATGGCAACTGGCCGCTG
GACCGCGGAAGGTCGCGAAA

Downstream 100 bases:

>100_bases
CCGGCGGCGCTCAGGGCCTGGCTGCGGCCTCCACCCAGGACTGCCGCTGGCCTGCCGAGCAGGAAGCCCTGCGCGAGATC
GCAGTTCATGCCCCTGAGGC

Product: putative methionine synthase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 880; Mature: 879

Protein sequence:

>880_residues
MTDATATFINIGERTNITGSAVFRKMITDGRYGDAVEVARQQVESGAQVIDVNMDEGMLDGVQAMTTFLNLIAAEPDIAR
VPVMIDSSKWEVIEAGLKCVQGKAIVNSISMKEGEDKFREQARACLSYGAAVVVMAFDEQGQADTAPRKIEICQRAFRIL
TEEVGFPAEDIIFDPNVFAVATGIEEHNTYALDFIEATREIRRTCPGCHISGGLSNVSFSFRGNEPVRRAMHSVFLYYAI
PAGMDMGIVNAGQLDIYDDIDRELREAVEDVILNRRADATDRLVDLAERFKGQKGKVQVKDLAWRELPVAKRLEHALVHG
ITEFIDTDTEEARLSVARPLHVIEGPLMAGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAWLEPYMEAEKRELGLERKSN
GKVILATVKGDVHDIGKNIVGVVLACNGYEIVDLGVMVPAEKILDTAVREKADIIGLSGLITPSLDEMVYVASEMQRRGM
VQPLLIGGATTSAAHTAVKIEPAIRSAPVVHVVDASRAVGVVSTLLSDEDRPVFLGEVRARYERMREARKDGPPKPRLPI
AEARAAKLKFDWDSYTPPRPTYEGVRTFEDIDLGTLARYIDWTPYFSAWDLAGRYPAILEDKIVGEAASSLWRDTQAMMQ
QLVGEDWLKPKAVVGFWKANSAGDDVKLANGEAFLTLRQQMMKDNSQPNFALADFVAPKGDDWIGGFCVTSGPEVEDIAQ
RFAAKGDDYSSIMVKALADRFAEAMAEYMHEKVRKELWAYAPDEALSTDELIAEKYQGIRPAAGYPCQPDHTEKATLFRL
LEAEKRIGVELTSSYAMMPASSVSGLYLSHPKSVYFAVGRIGRDQVADYAARKGWETRMAERWLAPILSYDPFVMADAVA

Sequences:

>Translated_880_residues
MTDATATFINIGERTNITGSAVFRKMITDGRYGDAVEVARQQVESGAQVIDVNMDEGMLDGVQAMTTFLNLIAAEPDIAR
VPVMIDSSKWEVIEAGLKCVQGKAIVNSISMKEGEDKFREQARACLSYGAAVVVMAFDEQGQADTAPRKIEICQRAFRIL
TEEVGFPAEDIIFDPNVFAVATGIEEHNTYALDFIEATREIRRTCPGCHISGGLSNVSFSFRGNEPVRRAMHSVFLYYAI
PAGMDMGIVNAGQLDIYDDIDRELREAVEDVILNRRADATDRLVDLAERFKGQKGKVQVKDLAWRELPVAKRLEHALVHG
ITEFIDTDTEEARLSVARPLHVIEGPLMAGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAWLEPYMEAEKRELGLERKSN
GKVILATVKGDVHDIGKNIVGVVLACNGYEIVDLGVMVPAEKILDTAVREKADIIGLSGLITPSLDEMVYVASEMQRRGM
VQPLLIGGATTSAAHTAVKIEPAIRSAPVVHVVDASRAVGVVSTLLSDEDRPVFLGEVRARYERMREARKDGPPKPRLPI
AEARAAKLKFDWDSYTPPRPTYEGVRTFEDIDLGTLARYIDWTPYFSAWDLAGRYPAILEDKIVGEAASSLWRDTQAMMQ
QLVGEDWLKPKAVVGFWKANSAGDDVKLANGEAFLTLRQQMMKDNSQPNFALADFVAPKGDDWIGGFCVTSGPEVEDIAQ
RFAAKGDDYSSIMVKALADRFAEAMAEYMHEKVRKELWAYAPDEALSTDELIAEKYQGIRPAAGYPCQPDHTEKATLFRL
LEAEKRIGVELTSSYAMMPASSVSGLYLSHPKSVYFAVGRIGRDQVADYAARKGWETRMAERWLAPILSYDPFVMADAVA
>Mature_879_residues
TDATATFINIGERTNITGSAVFRKMITDGRYGDAVEVARQQVESGAQVIDVNMDEGMLDGVQAMTTFLNLIAAEPDIARV
PVMIDSSKWEVIEAGLKCVQGKAIVNSISMKEGEDKFREQARACLSYGAAVVVMAFDEQGQADTAPRKIEICQRAFRILT
EEVGFPAEDIIFDPNVFAVATGIEEHNTYALDFIEATREIRRTCPGCHISGGLSNVSFSFRGNEPVRRAMHSVFLYYAIP
AGMDMGIVNAGQLDIYDDIDRELREAVEDVILNRRADATDRLVDLAERFKGQKGKVQVKDLAWRELPVAKRLEHALVHGI
TEFIDTDTEEARLSVARPLHVIEGPLMAGMNVVGDLFGSGKMFLPQVVKSARVMKQAVAWLEPYMEAEKRELGLERKSNG
KVILATVKGDVHDIGKNIVGVVLACNGYEIVDLGVMVPAEKILDTAVREKADIIGLSGLITPSLDEMVYVASEMQRRGMV
QPLLIGGATTSAAHTAVKIEPAIRSAPVVHVVDASRAVGVVSTLLSDEDRPVFLGEVRARYERMREARKDGPPKPRLPIA
EARAAKLKFDWDSYTPPRPTYEGVRTFEDIDLGTLARYIDWTPYFSAWDLAGRYPAILEDKIVGEAASSLWRDTQAMMQQ
LVGEDWLKPKAVVGFWKANSAGDDVKLANGEAFLTLRQQMMKDNSQPNFALADFVAPKGDDWIGGFCVTSGPEVEDIAQR
FAAKGDDYSSIMVKALADRFAEAMAEYMHEKVRKELWAYAPDEALSTDELIAEKYQGIRPAAGYPCQPDHTEKATLFRLL
EAEKRIGVELTSSYAMMPASSVSGLYLSHPKSVYFAVGRIGRDQVADYAARKGWETRMAERWLAPILSYDPFVMADAVA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=898, Percent_Identity=54.2316258351893, Blast_Score=944, Evalue=0.0,
Organism=Escherichia coli, GI1790450, Length=879, Percent_Identity=63.0261660978385, Blast_Score=1083, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17535405, Length=892, Percent_Identity=54.1479820627803, Blast_Score=934, Evalue=0.0,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 97122; Mature: 96991

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50974 ADOMET_ACTIVATION ; PS50972 PTERIN_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDATATFINIGERTNITGSAVFRKMITDGRYGDAVEVARQQVESGAQVIDVNMDEGMLD
CCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHH
GVQAMTTFLNLIAAEPDIARVPVMIDSSKWEVIEAGLKCVQGKAIVNSISMKEGEDKFRE
HHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHH
QARACLSYGAAVVVMAFDEQGQADTAPRKIEICQRAFRILTEEVGFPAEDIIFDPNVFAV
HHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHEEECCCCEEE
ATGIEEHNTYALDFIEATREIRRTCPGCHISGGLSNVSFSFRGNEPVRRAMHSVFLYYAI
EECCCCCCCEEHHHHHHHHHHHHHCCCCEECCCCCCEEEEECCCHHHHHHHHHHHHHHEE
PAGMDMGIVNAGQLDIYDDIDRELREAVEDVILNRRADATDRLVDLAERFKGQKGKVQVK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEH
DLAWRELPVAKRLEHALVHGITEFIDTDTEEARLSVARPLHVIEGPLMAGMNVVGDLFGS
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCC
GKMFLPQVVKSARVMKQAVAWLEPYMEAEKRELGLERKSNGKVILATVKGDVHDIGKNIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHE
GVVLACNGYEIVDLGVMVPAEKILDTAVREKADIIGLSGLITPSLDEMVYVASEMQRRGM
EEEEEECCCEEEEECEECCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCC
VQPLLIGGATTSAAHTAVKIEPAIRSAPVVHVVDASRAVGVVSTLLSDEDRPVFLGEVRA
CCEEEECCCCCCHHCEEEEECHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCEEHHHHHH
RYERMREARKDGPPKPRLPIAEARAAKLKFDWDSYTPPRPTYEGVRTFEDIDLGTLARYI
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHH
DWTPYFSAWDLAGRYPAILEDKIVGEAASSLWRDTQAMMQQLVGEDWLKPKAVVGFWKAN
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
SAGDDVKLANGEAFLTLRQQMMKDNSQPNFALADFVAPKGDDWIGGFCVTSGPEVEDIAQ
CCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEHHHCCCCCCCCCCEEEECCCCCHHHHHH
RFAAKGDDYSSIMVKALADRFAEAMAEYMHEKVRKELWAYAPDEALSTDELIAEKYQGIR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
PAAGYPCQPDHTEKATLFRLLEAEKRIGVELTSSYAMMPASSVSGLYLSHPKSVYFAVGR
CCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCEEECCCCCCCCEEECCCCCEEHHHHC
IGRDQVADYAARKGWETRMAERWLAPILSYDPFVMADAVA
CCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCCCC
>Mature Secondary Structure 
TDATATFINIGERTNITGSAVFRKMITDGRYGDAVEVARQQVESGAQVIDVNMDEGMLD
CCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCEEEEECCCCCHHH
GVQAMTTFLNLIAAEPDIARVPVMIDSSKWEVIEAGLKCVQGKAIVNSISMKEGEDKFRE
HHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCHHHHHH
QARACLSYGAAVVVMAFDEQGQADTAPRKIEICQRAFRILTEEVGFPAEDIIFDPNVFAV
HHHHHHHCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHEEECCCCEEE
ATGIEEHNTYALDFIEATREIRRTCPGCHISGGLSNVSFSFRGNEPVRRAMHSVFLYYAI
EECCCCCCCEEHHHHHHHHHHHHHCCCCEECCCCCCEEEEECCCHHHHHHHHHHHHHHEE
PAGMDMGIVNAGQLDIYDDIDRELREAVEDVILNRRADATDRLVDLAERFKGQKGKVQVK
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEH
DLAWRELPVAKRLEHALVHGITEFIDTDTEEARLSVARPLHVIEGPLMAGMNVVGDLFGS
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCHHHHCCHHHHHHHHHHHHHCC
GKMFLPQVVKSARVMKQAVAWLEPYMEAEKRELGLERKSNGKVILATVKGDVHDIGKNIV
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHHHHHE
GVVLACNGYEIVDLGVMVPAEKILDTAVREKADIIGLSGLITPSLDEMVYVASEMQRRGM
EEEEEECCCEEEEECEECCHHHHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHCCC
VQPLLIGGATTSAAHTAVKIEPAIRSAPVVHVVDASRAVGVVSTLLSDEDRPVFLGEVRA
CCEEEECCCCCCHHCEEEEECHHHCCCCEEEEECCHHHHHHHHHHHCCCCCCEEHHHHHH
RYERMREARKDGPPKPRLPIAEARAAKLKFDWDSYTPPRPTYEGVRTFEDIDLGTLARYI
HHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHCCHHHHHHHH
DWTPYFSAWDLAGRYPAILEDKIVGEAASSLWRDTQAMMQQLVGEDWLKPKAVVGFWKAN
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECC
SAGDDVKLANGEAFLTLRQQMMKDNSQPNFALADFVAPKGDDWIGGFCVTSGPEVEDIAQ
CCCCCEEECCCCHHHHHHHHHHHCCCCCCEEEHHHCCCCCCCCCCEEEECCCCCHHHHHH
RFAAKGDDYSSIMVKALADRFAEAMAEYMHEKVRKELWAYAPDEALSTDELIAEKYQGIR
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCC
PAAGYPCQPDHTEKATLFRLLEAEKRIGVELTSSYAMMPASSVSGLYLSHPKSVYFAVGR
CCCCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCEEECCCCCCCCEEECCCCCEEHHHHC
IGRDQVADYAARKGWETRMAERWLAPILSYDPFVMADAVA
CCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]