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The map label for this gene is nusA [H]

Identifier: 114799070

GI number: 114799070

Start: 99369

End: 101147

Strand: Direct

Name: nusA [H]

Synonym: HNE_0112

Alternate gene names: 114799070

Gene position: 99369-101147 (Clockwise)

Preceding gene: 114800120

Following gene: 114798650

Centisome position: 2.68

GC content: 61.1

Gene sequence:

>1779_bases
ATGAGCGCCATCGGCGTTTCAGCCAACCGGCTTGAAATCCTGCAGATCGCCAAAGCGGTCGCCGAGGAAAAATCGATTGA
TCAGCGCATCGTCATTGAGGCGATGCAGGAGGCAATCGAGAAGGCTGCCAAGGCCAAGTACGGCCAGGAGCACGACATCC
GCGCCAAGATCGATCCGGCGACGGGCGAACAGACCCTGTGGCGCATTCAGACGGTTGTGGATGACGAGACCTTTGAAGAC
GAGGCCAAGCAGCTGCGTCTTTCCGAAGCCAAGAGAATCGATGCGACCCTCGTGGCCGGCAGCGAGTTGAAGGAAGAACT
TCCGCCCTTCGACTTCGGCCGCGTAGCGGCGCAGACGGCCAAGCAGGTGATCACGCAGAAAGTGCGCGATGCCGAGCGTG
AGCGTCAGTATAATGAATACAAGGACCGTGTCGGCGAGATCGTCTCGGGTATCGTCAAGCGTGTCGAGTATGGTCATGTG
ATCGTCGATCTTGGCCGCGCCGAGGGGATCATCCGCCGCAATGACGGTATCCCGCGCGAAAACTTCCAGCCCAACGACCG
CGTCCGCGCTTACCTCTACAAGGTCAGCCGCGAAGTGAAGGGTCCGCAGATTTTCCTGTCGCGCGCTGCACCGGACTTCA
TGCGCAAGCTGTTCGCCCAGGAAGTGCCGGAAGTTTATGAAGGCGTGATCCAGATCAAGGCCTGCGCCCGTGATCCGGGC
TCGCGCGCCAAGATCGGCGTGATTTCCAACGACTCCTCGATCGATCCGGTCGGCGCCTGTGTCGGTATGCGCGGCGCGCG
CGTTCAGGCCGTTGTCGGTGAGCTTTCTGGCGAGAAGATCGACATCATTCCGTGGAGCTTCGACGCGGCGACCTTCATCG
TGAACGGTCTTCAGCCTGCCGAAGTTTCCAAGGTGGTGCTGGATGAAGACGAGCGCCGGGTTGAAGTTGTCGTGGCAGAC
GATCAGTTCCCGCTGGCAATCGGCCGCCGGGGCCAGAACGTGCGTCTCGCGTCGCAACTGACCGGCTGGCAGATCGACCT
GATCACGGAGTCGGCAGATTCCGAGCGCTACCAGAAAGAGTTCCAGGAGCGCACCGACCTGTTCATGAAAGCGCTGGATG
CGGACGAAACCCTTGCCCAGCTGCTGGCTTCGGAAGGCTTTGAGTCGGTCGAGGAGATCGCGTTCGTGGCGCCTGAGGAC
TTCATCACGATTGAGGGCTTCGACAAGGATGTGGCCGAAGAGCTGCAGGAGCGCGCCCGTGAGTATCTCGACCGCGTTGC
CACCGAAAACGACGCGAAGCGCCGGGAACTTGGTGTTGCTGACGAGGTGATGGAACTTGAAGGCGTCACCCCGTCCTTCG
CCGTGAAGCTCGGCGAGCAGGGGGTCAAGACGGTTGAAGACGTTGCGGGTCTCGTACCCGACGACATCACCGGCTATCGC
GAGCCTGGCCCGGATGGAAAGCCGGTCTGGGTCGAGGGCATCCTCAAGAAAGGCGAGATGCGCAAGGACGACGCCAACCT
GTTCGTCATGAAGGCCCGCGTCGCTGCTGGCTGGATCGAACCGGAAGCCATTGAAGAGATGTTGGCCCAGCAGCAGTCGA
CCGAGCCTGCACCGGAGCTTACCGAAGAGGAGCGTGCGCTCCTCGCGCTCGGCGAGCTCGGCAATTCGGGGAATGCTGGA
AGTGATGATGACGAAGAGGCCCTCGCCGAAGAGCTCGGCATTGACCTCTCCGAACTTGAATCGGAGGGCGGTGATGACGA
AGCCGCATCCCGAGAGTGA

Upstream 100 bases:

>100_bases
GAAATCTGCCCCCGCAGCCGGAAGACGACGAAGACATGCTCGCCGATTTTGAAATCGACGAGTCCGAAGACGAAGAAGAC
CCAGAGACAGGAGATGTCCA

Downstream 100 bases:

>100_bases
TCCCCGACTGATCGCAGAAAATGATGAGAATGGCCTGGGCGAGGAAGATGCTTCCTCGCCTGAGCGCCAGTGCGCGGTAA
CGCGCGAGCGGCTGCCGAAG

Product: transcription termination factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 592; Mature: 591

Protein sequence:

>592_residues
MSAIGVSANRLEILQIAKAVAEEKSIDQRIVIEAMQEAIEKAAKAKYGQEHDIRAKIDPATGEQTLWRIQTVVDDETFED
EAKQLRLSEAKRIDATLVAGSELKEELPPFDFGRVAAQTAKQVITQKVRDAERERQYNEYKDRVGEIVSGIVKRVEYGHV
IVDLGRAEGIIRRNDGIPRENFQPNDRVRAYLYKVSREVKGPQIFLSRAAPDFMRKLFAQEVPEVYEGVIQIKACARDPG
SRAKIGVISNDSSIDPVGACVGMRGARVQAVVGELSGEKIDIIPWSFDAATFIVNGLQPAEVSKVVLDEDERRVEVVVAD
DQFPLAIGRRGQNVRLASQLTGWQIDLITESADSERYQKEFQERTDLFMKALDADETLAQLLASEGFESVEEIAFVAPED
FITIEGFDKDVAEELQERAREYLDRVATENDAKRRELGVADEVMELEGVTPSFAVKLGEQGVKTVEDVAGLVPDDITGYR
EPGPDGKPVWVEGILKKGEMRKDDANLFVMKARVAAGWIEPEAIEEMLAQQQSTEPAPELTEEERALLALGELGNSGNAG
SDDDEEALAEELGIDLSELESEGGDDEAASRE

Sequences:

>Translated_592_residues
MSAIGVSANRLEILQIAKAVAEEKSIDQRIVIEAMQEAIEKAAKAKYGQEHDIRAKIDPATGEQTLWRIQTVVDDETFED
EAKQLRLSEAKRIDATLVAGSELKEELPPFDFGRVAAQTAKQVITQKVRDAERERQYNEYKDRVGEIVSGIVKRVEYGHV
IVDLGRAEGIIRRNDGIPRENFQPNDRVRAYLYKVSREVKGPQIFLSRAAPDFMRKLFAQEVPEVYEGVIQIKACARDPG
SRAKIGVISNDSSIDPVGACVGMRGARVQAVVGELSGEKIDIIPWSFDAATFIVNGLQPAEVSKVVLDEDERRVEVVVAD
DQFPLAIGRRGQNVRLASQLTGWQIDLITESADSERYQKEFQERTDLFMKALDADETLAQLLASEGFESVEEIAFVAPED
FITIEGFDKDVAEELQERAREYLDRVATENDAKRRELGVADEVMELEGVTPSFAVKLGEQGVKTVEDVAGLVPDDITGYR
EPGPDGKPVWVEGILKKGEMRKDDANLFVMKARVAAGWIEPEAIEEMLAQQQSTEPAPELTEEERALLALGELGNSGNAG
SDDDEEALAEELGIDLSELESEGGDDEAASRE
>Mature_591_residues
SAIGVSANRLEILQIAKAVAEEKSIDQRIVIEAMQEAIEKAAKAKYGQEHDIRAKIDPATGEQTLWRIQTVVDDETFEDE
AKQLRLSEAKRIDATLVAGSELKEELPPFDFGRVAAQTAKQVITQKVRDAERERQYNEYKDRVGEIVSGIVKRVEYGHVI
VDLGRAEGIIRRNDGIPRENFQPNDRVRAYLYKVSREVKGPQIFLSRAAPDFMRKLFAQEVPEVYEGVIQIKACARDPGS
RAKIGVISNDSSIDPVGACVGMRGARVQAVVGELSGEKIDIIPWSFDAATFIVNGLQPAEVSKVVLDEDERRVEVVVADD
QFPLAIGRRGQNVRLASQLTGWQIDLITESADSERYQKEFQERTDLFMKALDADETLAQLLASEGFESVEEIAFVAPEDF
ITIEGFDKDVAEELQERAREYLDRVATENDAKRRELGVADEVMELEGVTPSFAVKLGEQGVKTVEDVAGLVPDDITGYRE
PGPDGKPVWVEGILKKGEMRKDDANLFVMKARVAAGWIEPEAIEEMLAQQQSTEPAPELTEEERALLALGELGNSGNAGS
DDDEEALAEELGIDLSELESEGGDDEAASRE

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=466, Percent_Identity=44.4206008583691, Blast_Score=387, Evalue=1e-108,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010995
- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010214
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 65477; Mature: 65346

Theoretical pI: Translated: 4.23; Mature: 4.23

Prosite motif: PS50084 KH_TYPE_1 ; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAIGVSANRLEILQIAKAVAEEKSIDQRIVIEAMQEAIEKAAKAKYGQEHDIRAKIDPA
CCCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
TGEQTLWRIQTVVDDETFEDEAKQLRLSEAKRIDATLVAGSELKEELPPFDFGRVAAQTA
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHCCCCCHHHHHHHHH
KQVITQKVRDAERERQYNEYKDRVGEIVSGIVKRVEYGHVIVDLGRAEGIIRRNDGIPRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEECCCCCCCC
NFQPNDRVRAYLYKVSREVKGPQIFLSRAAPDFMRKLFAQEVPEVYEGVIQIKACARDPG
CCCCCHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SRAKIGVISNDSSIDPVGACVGMRGARVQAVVGELSGEKIDIIPWSFDAATFIVNGLQPA
CCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCHH
EVSKVVLDEDERRVEVVVADDQFPLAIGRRGQNVRLASQLTGWQIDLITESADSERYQKE
HHHHHHHCCCCCEEEEEEECCCCCHHCCCCCCCEEEHHHCCCCEEEEEECCCCHHHHHHH
FQERTDLFMKALDADETLAQLLASEGFESVEEIAFVAPEDFITIEGFDKDVAEELQERAR
HHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
EYLDRVATENDAKRRELGVADEVMELEGVTPSFAVKLGEQGVKTVEDVAGLVPDDITGYR
HHHHHHHCCCCHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EPGPDGKPVWVEGILKKGEMRKDDANLFVMKARVAAGWIEPEAIEEMLAQQQSTEPAPEL
CCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHCCCCCCCCH
TEEERALLALGELGNSGNAGSDDDEEALAEELGIDLSELESEGGDDEAASRE
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SAIGVSANRLEILQIAKAVAEEKSIDQRIVIEAMQEAIEKAAKAKYGQEHDIRAKIDPA
CCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCC
TGEQTLWRIQTVVDDETFEDEAKQLRLSEAKRIDATLVAGSELKEELPPFDFGRVAAQTA
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEECCHHHHHHCCCCCHHHHHHHHH
KQVITQKVRDAERERQYNEYKDRVGEIVSGIVKRVEYGHVIVDLGRAEGIIRRNDGIPRE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCEEECCCCCCCC
NFQPNDRVRAYLYKVSREVKGPQIFLSRAAPDFMRKLFAQEVPEVYEGVIQIKACARDPG
CCCCCHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
SRAKIGVISNDSSIDPVGACVGMRGARVQAVVGELSGEKIDIIPWSFDAATFIVNGLQPA
CCCEEEEECCCCCCCHHHHHHCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHHCCCCHH
EVSKVVLDEDERRVEVVVADDQFPLAIGRRGQNVRLASQLTGWQIDLITESADSERYQKE
HHHHHHHCCCCCEEEEEEECCCCCHHCCCCCCCEEEHHHCCCCEEEEEECCCCHHHHHHH
FQERTDLFMKALDADETLAQLLASEGFESVEEIAFVAPEDFITIEGFDKDVAEELQERAR
HHHHHHHHHHHHCHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHH
EYLDRVATENDAKRRELGVADEVMELEGVTPSFAVKLGEQGVKTVEDVAGLVPDDITGYR
HHHHHHHCCCCHHHHHCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
EPGPDGKPVWVEGILKKGEMRKDDANLFVMKARVAAGWIEPEAIEEMLAQQQSTEPAPEL
CCCCCCCCCHHHHHHHCCCCCCCCCCEEEEEEHHHHCCCCHHHHHHHHHHHCCCCCCCCH
TEEERALLALGELGNSGNAGSDDDEEALAEELGIDLSELESEGGDDEAASRE
HHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11557893 [H]