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The map label for this gene is lcfB [H]

Identifier: 114799065

GI number: 114799065

Start: 2032851

End: 2034461

Strand: Direct

Name: lcfB [H]

Synonym: HNE_1963

Alternate gene names: 114799065

Gene position: 2032851-2034461 (Clockwise)

Preceding gene: 114800428

Following gene: 114799823

Centisome position: 54.87

GC content: 59.16

Gene sequence:

>1611_bases
ATGGATTGGAATTTCGGTGACATGCTCGACGCGGTCGGCGATGCGCTAGGCCCCAATGACCCGGCGCTGATCCATGGGGA
CCGAGTGGTCAGCTGGCCAGAAATGATGGCGCGCTCCAACCGTATGGCCAGAGCGCTTCACAGCCTCGGCGCAAAACCTG
GCGATAAAGCAGGCTTCTACCTGCGCAACCAACCCGAATATATGGAAGCCCTCGCCGCCTGCTTCAAAGCGCGACTCGCC
CATGTGAACGTCAATTATCGCTACCGTGACGATGAACTTGTCTACATCTTCGACAATTCAGACTCGACCGTCGTGTTCTT
CGACACCGAATTTTCTGCGGAAGTCGAACGCGTGCGCGAACGACTGCCCAAGGTGATCGCCTGGGTCCAGATCGGTGGTG
GCGACATCCTCCCCTTCGCCCATGATTATGAAAAGCTCGCCTCAGAAGGTGATGCCTCCAAACTCAATATCCCCCGCTCT
GGTGACGACATGCTGCTGCTCTATACCGGCGGCACCACCGGGATGCCCAAGGGCGTGATGTGGACCCATTCCATCTGGCG
CGAAGCCGGCATGGAAGGCGTGCGCAAGATGGGGGGCCCGGTTCCGGAGAATCTCGAAGAGCATGTCCAGAACGCGCTGA
CCGTGGGCAAATTCTCGCGCCAGATCCCCGCCTGCCCGCTGATGCACGGCACCGGCCTGTTTACCGCCATGGGGGCCATG
CTGAATGGCGGCGCCATTGTAACGCTCACCGACAAACGCAAATTCGATCCCGTGAACCTCTGGGATACTGTCGATGCCAA
TGGCGTCACGAGCATGGCCATTGTCGGCGATGCGTTTGCCAAACCAATGCTCAAGACACTCGACGAGAATCCCGGTCGCT
GGAACCTCTCTAGCGTGCTCGCCATCATTTCGTCTGGCGTGATGTGGAGCTCGGAAGTCAAGCAAGGGCTTCTCCGCCAT
ATGCCTCAAGTTGCGCTGACCGACAGCTTCGGCGCGTCCGAAGCCGTCGGCTTCGGCTCCTCGATCACAACCGCCGATGG
CCGCACCAAGACCTCAAAGTTCGAGATCGGCCCAAGCTGCAAGGTGTTCACCGAAGATGGTCGCGAAGTTCTGCCCGGCA
GCGGAGAGCCAGGCTACATCGCGCGTGGCGGCGCTGTTCCGCTGGGTTACTACAAGGACCCAGAGAAAACGGAAAAGACG
TACAAGGTGTATGGCGGCGTGCGTTACGCCGTACCGGGCGACTGGTGCACCGTTGAAGCTGACGGCACGATCACCCTCCT
TGGCAGAGGTTCAAACTGCATCAATACGGCTGGTGAGAAAGTTTATCCTGAGGAAGTCGAAGAAGCGCTGAAATCTCACG
CATCGGTCACCGACGCCCTGGTTGTGGGCGTTCCGGATGACAAATGGGGTCAGGCGATTACGGCCGTCGTATCTCTGAAT
GGGAACGCGACCGAAGACGAGCTGCGCGCTTTCGTTCAGTCAAAGATCGCCCGCTACAAGGCGCCCAAGCGTATTCTGTT
CAAGGACAATCTTGGCCGCGCGCCGAACGGCAAGGCGGACTACAAGTCGATCAAGGCCTTTGCGCTGCAGGAACTCGGCA
TCCCCGGCTGA

Upstream 100 bases:

>100_bases
CCCTCCGGAGCAATCCGGGGGGCTGTTTACTTCGGGTCATGATTGCAGGCCCGCTCAGCCCTCCTTAGCATCGCGCAAAA
TGATACTGGCGGAGGAAACG

Downstream 100 bases:

>100_bases
AGGAATTAGTCGGCCTTGCGGCGCACCGTGCGGAATACCGCGCTGGCCCGTGCCGCGAGGCCACCATCTTCCTTGACGAT
CCTTGCGTTGACATGGATGA

Product: acyl-CoA synthetase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 536; Mature: 536

Protein sequence:

>536_residues
MDWNFGDMLDAVGDALGPNDPALIHGDRVVSWPEMMARSNRMARALHSLGAKPGDKAGFYLRNQPEYMEALAACFKARLA
HVNVNYRYRDDELVYIFDNSDSTVVFFDTEFSAEVERVRERLPKVIAWVQIGGGDILPFAHDYEKLASEGDASKLNIPRS
GDDMLLLYTGGTTGMPKGVMWTHSIWREAGMEGVRKMGGPVPENLEEHVQNALTVGKFSRQIPACPLMHGTGLFTAMGAM
LNGGAIVTLTDKRKFDPVNLWDTVDANGVTSMAIVGDAFAKPMLKTLDENPGRWNLSSVLAIISSGVMWSSEVKQGLLRH
MPQVALTDSFGASEAVGFGSSITTADGRTKTSKFEIGPSCKVFTEDGREVLPGSGEPGYIARGGAVPLGYYKDPEKTEKT
YKVYGGVRYAVPGDWCTVEADGTITLLGRGSNCINTAGEKVYPEEVEEALKSHASVTDALVVGVPDDKWGQAITAVVSLN
GNATEDELRAFVQSKIARYKAPKRILFKDNLGRAPNGKADYKSIKAFALQELGIPG

Sequences:

>Translated_536_residues
MDWNFGDMLDAVGDALGPNDPALIHGDRVVSWPEMMARSNRMARALHSLGAKPGDKAGFYLRNQPEYMEALAACFKARLA
HVNVNYRYRDDELVYIFDNSDSTVVFFDTEFSAEVERVRERLPKVIAWVQIGGGDILPFAHDYEKLASEGDASKLNIPRS
GDDMLLLYTGGTTGMPKGVMWTHSIWREAGMEGVRKMGGPVPENLEEHVQNALTVGKFSRQIPACPLMHGTGLFTAMGAM
LNGGAIVTLTDKRKFDPVNLWDTVDANGVTSMAIVGDAFAKPMLKTLDENPGRWNLSSVLAIISSGVMWSSEVKQGLLRH
MPQVALTDSFGASEAVGFGSSITTADGRTKTSKFEIGPSCKVFTEDGREVLPGSGEPGYIARGGAVPLGYYKDPEKTEKT
YKVYGGVRYAVPGDWCTVEADGTITLLGRGSNCINTAGEKVYPEEVEEALKSHASVTDALVVGVPDDKWGQAITAVVSLN
GNATEDELRAFVQSKIARYKAPKRILFKDNLGRAPNGKADYKSIKAFALQELGIPG
>Mature_536_residues
MDWNFGDMLDAVGDALGPNDPALIHGDRVVSWPEMMARSNRMARALHSLGAKPGDKAGFYLRNQPEYMEALAACFKARLA
HVNVNYRYRDDELVYIFDNSDSTVVFFDTEFSAEVERVRERLPKVIAWVQIGGGDILPFAHDYEKLASEGDASKLNIPRS
GDDMLLLYTGGTTGMPKGVMWTHSIWREAGMEGVRKMGGPVPENLEEHVQNALTVGKFSRQIPACPLMHGTGLFTAMGAM
LNGGAIVTLTDKRKFDPVNLWDTVDANGVTSMAIVGDAFAKPMLKTLDENPGRWNLSSVLAIISSGVMWSSEVKQGLLRH
MPQVALTDSFGASEAVGFGSSITTADGRTKTSKFEIGPSCKVFTEDGREVLPGSGEPGYIARGGAVPLGYYKDPEKTEKT
YKVYGGVRYAVPGDWCTVEADGTITLLGRGSNCINTAGEKVYPEEVEEALKSHASVTDALVVGVPDDKWGQAITAVVSLN
GNATEDELRAFVQSKIARYKAPKRILFKDNLGRAPNGKADYKSIKAFALQELGIPG

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=549, Percent_Identity=23.6794171220401, Blast_Score=115, Evalue=1e-25,
Organism=Homo sapiens, GI187761345, Length=553, Percent_Identity=25.3164556962025, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI187761343, Length=553, Percent_Identity=25.3164556962025, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI227499619, Length=534, Percent_Identity=23.7827715355805, Blast_Score=89, Evalue=8e-18,
Organism=Homo sapiens, GI38505220, Length=517, Percent_Identity=21.2765957446809, Blast_Score=89, Evalue=9e-18,
Organism=Homo sapiens, GI38524616, Length=512, Percent_Identity=24.0234375, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI58082049, Length=510, Percent_Identity=21.9607843137255, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI157311624, Length=512, Percent_Identity=22.0703125, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI157311622, Length=512, Percent_Identity=22.0703125, Blast_Score=82, Evalue=2e-15,
Organism=Homo sapiens, GI115511026, Length=145, Percent_Identity=33.7931034482759, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI145693145, Length=502, Percent_Identity=25.2988047808765, Blast_Score=115, Evalue=1e-26,
Organism=Escherichia coli, GI1788107, Length=544, Percent_Identity=25.5514705882353, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1786810, Length=544, Percent_Identity=22.6102941176471, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI221142682, Length=507, Percent_Identity=23.8658777120316, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790505, Length=144, Percent_Identity=28.4722222222222, Blast_Score=62, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17557194, Length=546, Percent_Identity=26.5567765567766, Blast_Score=146, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17538037, Length=513, Percent_Identity=26.9005847953216, Blast_Score=142, Evalue=6e-34,
Organism=Caenorhabditis elegans, GI71994703, Length=575, Percent_Identity=25.0434782608696, Blast_Score=122, Evalue=4e-28,
Organism=Caenorhabditis elegans, GI71994694, Length=575, Percent_Identity=25.0434782608696, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI71994690, Length=575, Percent_Identity=25.0434782608696, Blast_Score=122, Evalue=5e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=556, Percent_Identity=24.8201438848921, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=513, Percent_Identity=23.9766081871345, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17559526, Length=519, Percent_Identity=25.0481695568401, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17531443, Length=462, Percent_Identity=24.025974025974, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17558820, Length=356, Percent_Identity=25, Blast_Score=84, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17560140, Length=522, Percent_Identity=23.1800766283525, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI71985884, Length=375, Percent_Identity=24.2666666666667, Blast_Score=75, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI115534440, Length=567, Percent_Identity=22.2222222222222, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319699, Length=370, Percent_Identity=25.4054054054054, Blast_Score=96, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6319264, Length=551, Percent_Identity=24.1379310344828, Blast_Score=86, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6323182, Length=579, Percent_Identity=22.9706390328152, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24581924, Length=575, Percent_Identity=24.3478260869565, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI18859661, Length=527, Percent_Identity=26.1859582542694, Blast_Score=96, Evalue=8e-20,
Organism=Drosophila melanogaster, GI19922652, Length=517, Percent_Identity=22.8239845261122, Blast_Score=89, Evalue=6e-18,
Organism=Drosophila melanogaster, GI21355181, Length=495, Percent_Identity=22.4242424242424, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 58161; Mature: 58161

Theoretical pI: Translated: 5.55; Mature: 5.55

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDWNFGDMLDAVGDALGPNDPALIHGDRVVSWPEMMARSNRMARALHSLGAKPGDKAGFY
CCCCHHHHHHHHHHHCCCCCCEEEECCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
LRNQPEYMEALAACFKARLAHVNVNYRYRDDELVYIFDNSDSTVVFFDTEFSAEVERVRE
ECCCHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEEECCCCEEEEEECCHHHHHHHHHH
RLPKVIAWVQIGGGDILPFAHDYEKLASEGDASKLNIPRSGDDMLLLYTGGTTGMPKGVM
HHHHEEEEEEECCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCEEEEEECCCCCCCCCCC
WTHSIWREAGMEGVRKMGGPVPENLEEHVQNALTVGKFSRQIPACPLMHGTGLFTAMGAM
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
LNGGAIVTLTDKRKFDPVNLWDTVDANGVTSMAIVGDAFAKPMLKTLDENPGRWNLSSVL
HCCCEEEEEECCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
AIISSGVMWSSEVKQGLLRHMPQVALTDSFGASEAVGFGSSITTADGRTKTSKFEIGPSC
HHHHCCCEEHHHHHHHHHHHCCCEEEECCCCCCHHCCCCCCEECCCCCCCCCEEECCCCE
KVFTEDGREVLPGSGEPGYIARGGAVPLGYYKDPEKTEKTYKVYGGVRYAVPGDWCTVEA
EEEECCCCEECCCCCCCCEEECCCCCCCCCCCCCHHHHHHEEEECCEEEECCCCEEEEEC
DGTITLLGRGSNCINTAGEKVYPEEVEEALKSHASVTDALVVGVPDDKWGQAITAVVSLN
CCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHCCEEEEEEEEC
GNATEDELRAFVQSKIARYKAPKRILFKDNLGRAPNGKADYKSIKAFALQELGIPG
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MDWNFGDMLDAVGDALGPNDPALIHGDRVVSWPEMMARSNRMARALHSLGAKPGDKAGFY
CCCCHHHHHHHHHHHCCCCCCEEEECCEEECHHHHHHHHHHHHHHHHHHCCCCCCCCCEE
LRNQPEYMEALAACFKARLAHVNVNYRYRDDELVYIFDNSDSTVVFFDTEFSAEVERVRE
ECCCHHHHHHHHHHHHHHHHEEEEEEEECCCCEEEEEECCCCEEEEEECCHHHHHHHHHH
RLPKVIAWVQIGGGDILPFAHDYEKLASEGDASKLNIPRSGDDMLLLYTGGTTGMPKGVM
HHHHEEEEEEECCCCCCCCHHHHHHHHCCCCCCEECCCCCCCCEEEEEECCCCCCCCCCC
WTHSIWREAGMEGVRKMGGPVPENLEEHVQNALTVGKFSRQIPACPLMHGTGLFTAMGAM
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
LNGGAIVTLTDKRKFDPVNLWDTVDANGVTSMAIVGDAFAKPMLKTLDENPGRWNLSSVL
HCCCEEEEEECCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCCCCCHHHHH
AIISSGVMWSSEVKQGLLRHMPQVALTDSFGASEAVGFGSSITTADGRTKTSKFEIGPSC
HHHHCCCEEHHHHHHHHHHHCCCEEEECCCCCCHHCCCCCCEECCCCCCCCCEEECCCCE
KVFTEDGREVLPGSGEPGYIARGGAVPLGYYKDPEKTEKTYKVYGGVRYAVPGDWCTVEA
EEEECCCCEECCCCCCCCEEECCCCCCCCCCCCCHHHHHHEEEECCEEEECCCCEEEEEC
DGTITLLGRGSNCINTAGEKVYPEEVEEALKSHASVTDALVVGVPDDKWGQAITAVVSLN
CCEEEEEECCCCHHHCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHCCEEEEEEEEC
GNATEDELRAFVQSKIARYKAPKRILFKDNLGRAPNGKADYKSIKAFALQELGIPG
CCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]