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The map label for this gene is purD [H]

Identifier: 114799064

GI number: 114799064

Start: 939626

End: 940918

Strand: Direct

Name: purD [H]

Synonym: HNE_0922

Alternate gene names: 114799064

Gene position: 939626-940918 (Clockwise)

Preceding gene: 114798268

Following gene: 114798572

Centisome position: 25.36

GC content: 64.58

Gene sequence:

>1293_bases
ATGAACATACTCCTGATCGGATCGGGCGGCCGTGAACACGCCCTGGCCTGGAAGATGGCGCAATCGCCGCTGGTAGACGT
GGTGCATTGCGCGCCCGGCAATCCCGGCATGGACGAGATTGGCCCCTGTTTTGACGTGGCCGCCGATGATGTGGACGGCC
TCGTAAAGCTCGCCCTCCAGATCGAGCCGGACCTGATCGTGATTGGCCCCGAGGCCCCCCTCGCGCTCGGCCTGTCAGAC
ATCCTGCGCGCCCGTGGCTTTGACGTCTTCGGCCCCTCCAGAGAGGCCGCCCAGCTGGAAGCCTCTAAGGCCTTCTCCAA
GGCGCGGATGACGAAATACGGCGTCCCAACAGCGGCCTATGATGAGTTTACCGCTGCCGCCCCCGCCAAGGCCTTCCTGC
GCAAGATGCGCGCGCCTTATGTGCTGAAAGCCGATGGCCTCGCTGCCGGCAAAGGCGTGGTGATCGCGCAGACGCTGGAA
GAGGCAGACGCCGAAATCGACGAGATGCTGTCGGGCAAGTTCGGCTCGGCCTCTGCCACTCTGGTGATCGAAGAGTTCAT
GGAGGGCGAGGAAGCCAGCGTGTTCGTCATCACCGATGGCGAAGGCGCGATCTATCTGCCCGCCGCGCAGGACCATAAGC
GCGTCGGCGGTGGCGACACCGGCCCCAACACCGGCGGCATGGGCGCCTATGCCCCCGCCCCGGTCGTCAGTGAAGAGATC
ATGGCGCGCGTAAAGTGCGAGATTGCCGCGCCGATGCTGAAGGGCATGGCGAAAGACGGCATGCCCTATCAGGGCGTGCT
CTATATCGGCGTCATGGTCACGCCCGAAGGCCCCAAGGTTGTCGAGTTCAATGCGCGCTTCGGAGACCCTGAATGCCAGG
TGCTGATGAAGGGCCTGACGGGCGACATTGTTCCGGCGATCCTCGTGGCGGCAACCGGCGGCCTGAAGGGCGCCGAAGAG
GCGTTTGAAGCGCTGCTGGACCTGGACAACTTCCAGCCGGCCGCCACCGTCGTGCTCGCCGCTAACGGCTATCCGGGCAG
CTATGAAAAAGGCAGCGTCATTGAGGGCGTCAAGGAGGCAAGCGCGCTCGAAGGCGTCACCGTTTTTCATGCGGGCACGG
ATGTTACCGAAGGCGGAAAGCTCGTCGCCAAAGGCGGGCGCGTGTTGAACGTCACTGCCAGCGGGTCCACCTTGCGCGAA
GCGATCGACCGTGCCTATGCGGGCGTGGACGCCATTGACTGGCCGGGCGGTTTCTGCCGCCGGGACATTGGCTGGCGGGC
GCTGAAGCCTTAA

Upstream 100 bases:

>100_bases
CGACATTTCTTAAAGTTACGGTGAAGGTGTGAGGCCATAACGGCGCAGATCGGCGAGTTGCTCTTGATCTTGGCGGCGCT
CCCGCGCAACAAGCCGCCCC

Downstream 100 bases:

>100_bases
GCGTTCGGATCAATCAGTATCTTGATTTCGCTGGGGCTGCTGGCGAGGCTGTCGAAGGCGGCTTCGGTGTTCGCCAGCGG
TTGACGGCTGGTGATGAGGC

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 430; Mature: 430

Protein sequence:

>430_residues
MNILLIGSGGREHALAWKMAQSPLVDVVHCAPGNPGMDEIGPCFDVAADDVDGLVKLALQIEPDLIVIGPEAPLALGLSD
ILRARGFDVFGPSREAAQLEASKAFSKARMTKYGVPTAAYDEFTAAAPAKAFLRKMRAPYVLKADGLAAGKGVVIAQTLE
EADAEIDEMLSGKFGSASATLVIEEFMEGEEASVFVITDGEGAIYLPAAQDHKRVGGGDTGPNTGGMGAYAPAPVVSEEI
MARVKCEIAAPMLKGMAKDGMPYQGVLYIGVMVTPEGPKVVEFNARFGDPECQVLMKGLTGDIVPAILVAATGGLKGAEE
AFEALLDLDNFQPAATVVLAANGYPGSYEKGSVIEGVKEASALEGVTVFHAGTDVTEGGKLVAKGGRVLNVTASGSTLRE
AIDRAYAGVDAIDWPGGFCRRDIGWRALKP

Sequences:

>Translated_430_residues
MNILLIGSGGREHALAWKMAQSPLVDVVHCAPGNPGMDEIGPCFDVAADDVDGLVKLALQIEPDLIVIGPEAPLALGLSD
ILRARGFDVFGPSREAAQLEASKAFSKARMTKYGVPTAAYDEFTAAAPAKAFLRKMRAPYVLKADGLAAGKGVVIAQTLE
EADAEIDEMLSGKFGSASATLVIEEFMEGEEASVFVITDGEGAIYLPAAQDHKRVGGGDTGPNTGGMGAYAPAPVVSEEI
MARVKCEIAAPMLKGMAKDGMPYQGVLYIGVMVTPEGPKVVEFNARFGDPECQVLMKGLTGDIVPAILVAATGGLKGAEE
AFEALLDLDNFQPAATVVLAANGYPGSYEKGSVIEGVKEASALEGVTVFHAGTDVTEGGKLVAKGGRVLNVTASGSTLRE
AIDRAYAGVDAIDWPGGFCRRDIGWRALKP
>Mature_430_residues
MNILLIGSGGREHALAWKMAQSPLVDVVHCAPGNPGMDEIGPCFDVAADDVDGLVKLALQIEPDLIVIGPEAPLALGLSD
ILRARGFDVFGPSREAAQLEASKAFSKARMTKYGVPTAAYDEFTAAAPAKAFLRKMRAPYVLKADGLAAGKGVVIAQTLE
EADAEIDEMLSGKFGSASATLVIEEFMEGEEASVFVITDGEGAIYLPAAQDHKRVGGGDTGPNTGGMGAYAPAPVVSEEI
MARVKCEIAAPMLKGMAKDGMPYQGVLYIGVMVTPEGPKVVEFNARFGDPECQVLMKGLTGDIVPAILVAATGGLKGAEE
AFEALLDLDNFQPAATVVLAANGYPGSYEKGSVIEGVKEASALEGVTVFHAGTDVTEGGKLVAKGGRVLNVTASGSTLRE
AIDRAYAGVDAIDWPGGFCRRDIGWRALKP

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=431, Percent_Identity=46.4037122969838, Blast_Score=356, Evalue=3e-98,
Organism=Homo sapiens, GI4503915, Length=431, Percent_Identity=46.4037122969838, Blast_Score=356, Evalue=3e-98,
Organism=Homo sapiens, GI209869995, Length=431, Percent_Identity=46.4037122969838, Blast_Score=356, Evalue=3e-98,
Organism=Homo sapiens, GI209869993, Length=431, Percent_Identity=46.4037122969838, Blast_Score=356, Evalue=3e-98,
Organism=Escherichia coli, GI1790438, Length=434, Percent_Identity=49.5391705069124, Blast_Score=398, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI17567511, Length=429, Percent_Identity=41.025641025641, Blast_Score=279, Evalue=2e-75,
Organism=Saccharomyces cerevisiae, GI6321203, Length=447, Percent_Identity=41.1633109619687, Blast_Score=323, Evalue=2e-89,
Organism=Drosophila melanogaster, GI62471619, Length=439, Percent_Identity=42.8246013667426, Blast_Score=307, Evalue=7e-84,
Organism=Drosophila melanogaster, GI24582400, Length=439, Percent_Identity=42.8246013667426, Blast_Score=307, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 44730; Mature: 44730

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILLIGSGGREHALAWKMAQSPLVDVVHCAPGNPGMDEIGPCFDVAADDVDGLVKLALQ
CEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHCCHHHHHHCCHHHHEEEEEE
IEPDLIVIGPEAPLALGLSDILRARGFDVFGPSREAAQLEASKAFSKARMTKYGVPTAAY
ECCCEEEECCCCCHHHCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHH
DEFTAAAPAKAFLRKMRAPYVLKADGLAAGKGVVIAQTLEEADAEIDEMLSGKFGSASAT
HHHHHCCCHHHHHHHHCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCHHH
LVIEEFMEGEEASVFVITDGEGAIYLPAAQDHKRVGGGDTGPNTGGMGAYAPAPVVSEEI
HHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
MARVKCEIAAPMLKGMAKDGMPYQGVLYIGVMVTPEGPKVVEFNARFGDPECQVLMKGLT
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHCC
GDIVPAILVAATGGLKGAEEAFEALLDLDNFQPAATVVLAANGYPGSYEKGSVIEGVKEA
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHH
SALEGVTVFHAGTDVTEGGKLVAKGGRVLNVTASGSTLREAIDRAYAGVDAIDWPGGFCR
HHHCCEEEEECCCCCCCCCEEEECCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCHHH
RDIGWRALKP
HCCCCCCCCC
>Mature Secondary Structure
MNILLIGSGGREHALAWKMAQSPLVDVVHCAPGNPGMDEIGPCFDVAADDVDGLVKLALQ
CEEEEEECCCCCCHHHHHHHCCCCEEEEEECCCCCCHHHHCCHHHHHHCCHHHHEEEEEE
IEPDLIVIGPEAPLALGLSDILRARGFDVFGPSREAAQLEASKAFSKARMTKYGVPTAAY
ECCCEEEECCCCCHHHCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHH
DEFTAAAPAKAFLRKMRAPYVLKADGLAAGKGVVIAQTLEEADAEIDEMLSGKFGSASAT
HHHHHCCCHHHHHHHHCCCEEEEECCCCCCCCEEEEEHHHHHHHHHHHHHCCCCCCCHHH
LVIEEFMEGEEASVFVITDGEGAIYLPAAQDHKRVGGGDTGPNTGGMGAYAPAPVVSEEI
HHHHHHHCCCCCEEEEEECCCCEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
MARVKCEIAAPMLKGMAKDGMPYQGVLYIGVMVTPEGPKVVEFNARFGDPECQVLMKGLT
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEECCCCCEEEEEECCCCCHHHHHHHHHCC
GDIVPAILVAATGGLKGAEEAFEALLDLDNFQPAATVVLAANGYPGSYEKGSVIEGVKEA
HHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHH
SALEGVTVFHAGTDVTEGGKLVAKGGRVLNVTASGSTLREAIDRAYAGVDAIDWPGGFCR
HHHCCEEEEECCCCCCCCCEEEECCCEEEEEEECCHHHHHHHHHHHCCCCCCCCCCCHHH
RDIGWRALKP
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11743193; 11743194 [H]