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The map label for this gene is ntrX [H]

Identifier: 114799003

GI number: 114799003

Start: 2084313

End: 2085698

Strand: Reverse

Name: ntrX [H]

Synonym: HNE_2009

Alternate gene names: 114799003

Gene position: 2085698-2084313 (Counterclockwise)

Preceding gene: 114800247

Following gene: 114799538

Centisome position: 56.29

GC content: 63.35

Gene sequence:

>1386_bases
ATGGCACTCGACATCCTGGTAGTGGACGATGAGCCTGACATCCGTGAGCTGATGGCAGGTGTCCTCGGCGACGAAGGTTA
TGCTGTCCGTACCGCAGACACCGCCGAGCGCGCGCTGGAGGAAGTCCGCTCGCGCACGCCCCGTCTCGTGATCCTCGACG
TGTGGCTGCAGGGCAGCGGCATGGATGGTCTCTCGCTCCTGAAATATCTCAAGTCGATTGACCCGATCCTTCCGGTGATC
GTCATCAGCGGGCACGGCAATATCGAAACGGCCATCGCCGCCATCCGGCGCGGGGCATTCGATTTTATCGAAAAGCCGAT
CAAGGCAGATCGCCTGATGCTGGTTGTGGAGCGCGCCATCGAAGCGGCCGCGCTGCGCTATGAGAATGCCGCGCTGCGCA
ATCGCACGGGCGATGAGGAGCGCTGGATCGGCAACAGCCAGGCCGCCGCTGCCCTGCGCGCGTCGCTGGACAAGGTCGCG
CCGACCAATTCGCGCGTCCTCATCACTGGCCCCGCTGGCGTCGGCAAGGAGCTCGCCGCCCGTCTTATTCACAAGAACTC
CAGCCGCGCCGATGGCCCGTTTGTCGTCGTCAATGCCGCTACCATTGCGCCCGATCAGATGGAGATTGCCCTTTTCGGAG
AGGAAGATCAGACCGGGCGGACCGTGCGCGTCGGCCTGTTCGAGCGCGCGCATACCGGCACGCTGCTGCTCGACGAAGTC
GCCGACATGCCCATCGGCACGCAGAACAAGATCCTTCGCGTGCTGACAGAGCAGCGCTTTCAGCGCGTCGGCGGGCGGTC
CGATGTCAGTGTAGATGTCCGCGTCATGGCGGCCACCACCAAAGACCTCAAAGCGGAGATCGACGCCGGAAATTTCCGCG
AAGACCTCTATTACCGCCTCAGTGTCGTGCCGCTGCGCGTGCCCTCGCTGTCCGAGCGCCGGGACGACATTGTCGAGCTC
GCCAACTATTTCTGCGAACGCATCGCCGAGACCTCCGGCCTCACCTATCGCAGTTTCACGACCGAGGCCCTGGCCGTGCT
CCAGTCGATGGAGTGGCCGGGAAATATCCGCCAGCTTCGCAATATGGTCGAGCGCATCCTGATCCTCTCGCCGGCGGATT
CGCCCCGTCCGATCAGCGTGGATGAGCTGCCGCGCGATCAGGGCCGGGGGCCGTCGCCCGCGGCAAAAGCGGGCTCTGCC
GAGCTGGTGGGCCTCTCCCTGCGTGACGCCCGCGAGCAGTTCGAGCGGGAATATCTTACCCTGCAGATCACCCGTTTTAA
CGGAAACATCTCGCGGACGGCCGAGTTCATCGGTATGGAGCGCTCAGCACTGCACCGAAAATTAAAGGCTTTGGGAATAT
CCACGGGCGTTCGTCACGATACCTGA

Upstream 100 bases:

>100_bases
AACGGCGCGCTGGTGATGCAGTCAGAAGACAATTCCGCAGACGAGGTGGGCGGGACTGACCGCTTACCTTATCCCTTGAG
TTTGTAGAGGAGAGTAGCGC

Downstream 100 bases:

>100_bases
GCCAGACGCTGAAGGCATGAAGATATGCACGTTCTGATCTGCGGCGCCGGGCGGGTGGGGCAGGGCATTGCCCGCCGCCT
CGCACGGGAAAAGCACGAGA

Product: sigma-54 dependent DNA-binding response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 460

Protein sequence:

>461_residues
MALDILVVDDEPDIRELMAGVLGDEGYAVRTADTAERALEEVRSRTPRLVILDVWLQGSGMDGLSLLKYLKSIDPILPVI
VISGHGNIETAIAAIRRGAFDFIEKPIKADRLMLVVERAIEAAALRYENAALRNRTGDEERWIGNSQAAAALRASLDKVA
PTNSRVLITGPAGVGKELAARLIHKNSSRADGPFVVVNAATIAPDQMEIALFGEEDQTGRTVRVGLFERAHTGTLLLDEV
ADMPIGTQNKILRVLTEQRFQRVGGRSDVSVDVRVMAATTKDLKAEIDAGNFREDLYYRLSVVPLRVPSLSERRDDIVEL
ANYFCERIAETSGLTYRSFTTEALAVLQSMEWPGNIRQLRNMVERILILSPADSPRPISVDELPRDQGRGPSPAAKAGSA
ELVGLSLRDAREQFEREYLTLQITRFNGNISRTAEFIGMERSALHRKLKALGISTGVRHDT

Sequences:

>Translated_461_residues
MALDILVVDDEPDIRELMAGVLGDEGYAVRTADTAERALEEVRSRTPRLVILDVWLQGSGMDGLSLLKYLKSIDPILPVI
VISGHGNIETAIAAIRRGAFDFIEKPIKADRLMLVVERAIEAAALRYENAALRNRTGDEERWIGNSQAAAALRASLDKVA
PTNSRVLITGPAGVGKELAARLIHKNSSRADGPFVVVNAATIAPDQMEIALFGEEDQTGRTVRVGLFERAHTGTLLLDEV
ADMPIGTQNKILRVLTEQRFQRVGGRSDVSVDVRVMAATTKDLKAEIDAGNFREDLYYRLSVVPLRVPSLSERRDDIVEL
ANYFCERIAETSGLTYRSFTTEALAVLQSMEWPGNIRQLRNMVERILILSPADSPRPISVDELPRDQGRGPSPAAKAGSA
ELVGLSLRDAREQFEREYLTLQITRFNGNISRTAEFIGMERSALHRKLKALGISTGVRHDT
>Mature_460_residues
ALDILVVDDEPDIRELMAGVLGDEGYAVRTADTAERALEEVRSRTPRLVILDVWLQGSGMDGLSLLKYLKSIDPILPVIV
ISGHGNIETAIAAIRRGAFDFIEKPIKADRLMLVVERAIEAAALRYENAALRNRTGDEERWIGNSQAAAALRASLDKVAP
TNSRVLITGPAGVGKELAARLIHKNSSRADGPFVVVNAATIAPDQMEIALFGEEDQTGRTVRVGLFERAHTGTLLLDEVA
DMPIGTQNKILRVLTEQRFQRVGGRSDVSVDVRVMAATTKDLKAEIDAGNFREDLYYRLSVVPLRVPSLSERRDDIVELA
NYFCERIAETSGLTYRSFTTEALAVLQSMEWPGNIRQLRNMVERILILSPADSPRPISVDELPRDQGRGPSPAAKAGSAE
LVGLSLRDAREQFEREYLTLQITRFNGNISRTAEFIGMERSALHRKLKALGISTGVRHDT

Specific function: Member of the two-component regulatory system ntrY/ntrX involved in the activation of nitrogen assimilatory genes such as glnA. NtrX is probably phosphorylated by ntrY and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=458, Percent_Identity=37.3362445414847, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1790437, Length=451, Percent_Identity=37.9157427937916, Blast_Score=279, Evalue=2e-76,
Organism=Escherichia coli, GI1790299, Length=472, Percent_Identity=35.3813559322034, Blast_Score=276, Evalue=2e-75,
Organism=Escherichia coli, GI1788905, Length=453, Percent_Identity=35.7615894039735, Blast_Score=257, Evalue=1e-69,
Organism=Escherichia coli, GI87082152, Length=323, Percent_Identity=38.0804953560372, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI87082117, Length=322, Percent_Identity=37.5776397515528, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI1789087, Length=317, Percent_Identity=38.4858044164038, Blast_Score=185, Evalue=6e-48,
Organism=Escherichia coli, GI1786524, Length=363, Percent_Identity=35.2617079889807, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1789233, Length=319, Percent_Identity=33.8557993730408, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI87081872, Length=313, Percent_Identity=35.7827476038339, Blast_Score=169, Evalue=4e-43,
Organism=Escherichia coli, GI1787583, Length=297, Percent_Identity=36.026936026936, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI87081858, Length=291, Percent_Identity=31.6151202749141, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1789828, Length=224, Percent_Identity=34.8214285714286, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1786599, Length=161, Percent_Identity=29.1925465838509, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 50789; Mature: 50658

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALDILVVDDEPDIRELMAGVLGDEGYAVRTADTAERALEEVRSRTPRLVILDVWLQGSG
CEEEEEEECCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCC
MDGLSLLKYLKSIDPILPVIVISGHGNIETAIAAIRRGAFDFIEKPIKADRLMLVVERAI
CCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EAAALRYENAALRNRTGDEERWIGNSQAAAALRASLDKVAPTNSRVLITGPAGVGKELAA
HHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
RLIHKNSSRADGPFVVVNAATIAPDQMEIALFGEEDQTGRTVRVGLFERAHTGTLLLDEV
HHHHCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEEEEEECCCCCCCEEHHHH
ADMPIGTQNKILRVLTEQRFQRVGGRSDVSVDVRVMAATTKDLKAEIDAGNFREDLYYRL
HCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHHEECCCCCCHHHHEEEE
SVVPLRVPSLSERRDDIVELANYFCERIAETSGLTYRSFTTEALAVLQSMEWPGNIRQLR
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCHHHHHH
NMVERILILSPADSPRPISVDELPRDQGRGPSPAAKAGSAELVGLSLRDAREQFEREYLT
HHHHHHEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCEEE
LQITRFNGNISRTAEFIGMERSALHRKLKALGISTGVRHDT
EEEEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ALDILVVDDEPDIRELMAGVLGDEGYAVRTADTAERALEEVRSRTPRLVILDVWLQGSG
EEEEEEECCCCCHHHHHHHHHCCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEEECCCC
MDGLSLLKYLKSIDPILPVIVISGHGNIETAIAAIRRGAFDFIEKPIKADRLMLVVERAI
CCHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
EAAALRYENAALRNRTGDEERWIGNSQAAAALRASLDKVAPTNSRVLITGPAGVGKELAA
HHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCHHHHH
RLIHKNSSRADGPFVVVNAATIAPDQMEIALFGEEDQTGRTVRVGLFERAHTGTLLLDEV
HHHHCCCCCCCCCEEEEEEEEECCCCEEEEEECCCCCCCCEEEEEEECCCCCCCEEHHHH
ADMPIGTQNKILRVLTEQRFQRVGGRSDVSVDVRVMAATTKDLKAEIDAGNFREDLYYRL
HCCCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCHHHHEECCCCCCHHHHEEEE
SVVPLRVPSLSERRDDIVELANYFCERIAETSGLTYRSFTTEALAVLQSMEWPGNIRQLR
EEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHCCCCCHHHHHH
NMVERILILSPADSPRPISVDELPRDQGRGPSPAAKAGSAELVGLSLRDAREQFEREYLT
HHHHHHEEECCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCEEE
LQITRFNGNISRTAEFIGMERSALHRKLKALGISTGVRHDT
EEEEEECCCHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1661370 [H]