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The map label for this gene is gadB [C]

Identifier: 114798800

GI number: 114798800

Start: 3112624

End: 3113862

Strand: Direct

Name: gadB [C]

Synonym: HNE_2956

Alternate gene names: 114798800

Gene position: 3112624-3113862 (Clockwise)

Preceding gene: 114798324

Following gene: 114799809

Centisome position: 84.01

GC content: 63.52

Gene sequence:

>1239_bases
ATGCAGATGCCCGCTGAAGGACGCCCATGGGACGAGGTGCGCGCAGACATGCTGGCGCGCGGCGCAGGCGATGTTGCGTG
GCGCGACGGCAAGACAGCCGTCTACGTTTTCAATGCCGGCGAAGATGTGCACGCCCTCCAGCACGAAGCCTATGGCCTGT
TCATGGCGGAAAATGGCCTCGGCCCGCTCGCCTTTCCGTCGCTCGCGCAGATGGAGAAGGATGTGATCTCCATGGCGCTT
GGCCTGCTGCACGGACCAGAGGGATCAACCGGCGCCATCACATCGGGCGGCACCGATTCGATCACCATGGCGATCAAGAC
AGCGCGCGACTATGCCCGCGCCAAGGGCATGGCGAAGGATCGCCACAACATCGTCATCCCGCGCTCGGGTCACCTGGCCT
TCCACAAGGCGGCGTTGCTGATGGACATTGAAATCCGCAGCGTGCCGCTCAAGACTGACGGCAGCTACGAAGCCGATCCT
GCCGCCATGGCGGCCGCGATTGATGGCGCGACGATCATGATGGTCGGCTCAGCGCCAAACTTCCCGCATGGCATCATCGA
CCCGATCGCAGAGCTCGGCAAAATCGCTGAAGAGAAAGATGTGTGGTTGCATGTCGACGCCTGTGTCGGCGGTTATTTCG
CGCCGTTTGCGCGCATGAACGGCGTGCCGGTGCCGGACTTTGATTTCGCCATTCCAGCAGTGAAGTCCATCAGCGCGGAC
CTGCACAAATACGGCTACTGCGCCAAGGGCGCCTCAACCGTGCTGTTCCGGTCCGTCGATCTCTACAAGCATATGCCATT
TTCGCTCAGCGAATGGTCGGGCGCGCCGATGAAAACGCCGACGCTGGCGGGCACAAGGCCCGGCGGCGCGATCTCGGCAG
CCTGGGCGGTGATGAATGTTCTGGGTATTTCGGGCTACCGGGAGAAGCAGGGCCTCGTCTGCCAGACGCGCGAGCGGATC
GAAGCCGGCGTGAATGCGCTCGGCTTTGAAGTGCTTGGCAAGCCGCTCCTCGGCCTCGTCGCCTTCCGCCATCCGCAGGC
AGACACGCTCGCCCTCTACAGCGCCATGCGGCAGCGCGGCTGGTTCACTTCCTTCACGGTTGAGCCGCCGAGCCTGCATC
TCATGCTCTCGCCCAAGCATGCCGAAGTGGCAGACGATTATCTCGCCGACCTCGCCGCCAGCCTTGAAGCGGTGAAGGCG
GGAGACACCGCGCCGAAGGTGGAGGCGCGCTACTCCTGA

Upstream 100 bases:

>100_bases
CTTTTCCCCAGCGCTTTTCTGGATACACGCCGGCAGCGCCCGAATGGCTGGCCAGCCAAGGGCTTAGCCCCTACTCTCTT
CTCAAAAGAGGAGGACCGCC

Downstream 100 bases:

>100_bases
TCCAGAGCAGGTGTTGCGCCGCCTCCTCCTCCATGTTCCATTATCCGGCAACGAAACCCGCCGGGACGCGGCGGCACATG
TGGAGGACAACAGATGAAAT

Product: pyridoxal-dependent decarboxylase domain-containing protein

Products: NA

Alternate protein names: SP-lyase; SPL; Sphingosine-1-phosphate aldolase [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MQMPAEGRPWDEVRADMLARGAGDVAWRDGKTAVYVFNAGEDVHALQHEAYGLFMAENGLGPLAFPSLAQMEKDVISMAL
GLLHGPEGSTGAITSGGTDSITMAIKTARDYARAKGMAKDRHNIVIPRSGHLAFHKAALLMDIEIRSVPLKTDGSYEADP
AAMAAAIDGATIMMVGSAPNFPHGIIDPIAELGKIAEEKDVWLHVDACVGGYFAPFARMNGVPVPDFDFAIPAVKSISAD
LHKYGYCAKGASTVLFRSVDLYKHMPFSLSEWSGAPMKTPTLAGTRPGGAISAAWAVMNVLGISGYREKQGLVCQTRERI
EAGVNALGFEVLGKPLLGLVAFRHPQADTLALYSAMRQRGWFTSFTVEPPSLHLMLSPKHAEVADDYLADLAASLEAVKA
GDTAPKVEARYS

Sequences:

>Translated_412_residues
MQMPAEGRPWDEVRADMLARGAGDVAWRDGKTAVYVFNAGEDVHALQHEAYGLFMAENGLGPLAFPSLAQMEKDVISMAL
GLLHGPEGSTGAITSGGTDSITMAIKTARDYARAKGMAKDRHNIVIPRSGHLAFHKAALLMDIEIRSVPLKTDGSYEADP
AAMAAAIDGATIMMVGSAPNFPHGIIDPIAELGKIAEEKDVWLHVDACVGGYFAPFARMNGVPVPDFDFAIPAVKSISAD
LHKYGYCAKGASTVLFRSVDLYKHMPFSLSEWSGAPMKTPTLAGTRPGGAISAAWAVMNVLGISGYREKQGLVCQTRERI
EAGVNALGFEVLGKPLLGLVAFRHPQADTLALYSAMRQRGWFTSFTVEPPSLHLMLSPKHAEVADDYLADLAASLEAVKA
GDTAPKVEARYS
>Mature_412_residues
MQMPAEGRPWDEVRADMLARGAGDVAWRDGKTAVYVFNAGEDVHALQHEAYGLFMAENGLGPLAFPSLAQMEKDVISMAL
GLLHGPEGSTGAITSGGTDSITMAIKTARDYARAKGMAKDRHNIVIPRSGHLAFHKAALLMDIEIRSVPLKTDGSYEADP
AAMAAAIDGATIMMVGSAPNFPHGIIDPIAELGKIAEEKDVWLHVDACVGGYFAPFARMNGVPVPDFDFAIPAVKSISAD
LHKYGYCAKGASTVLFRSVDLYKHMPFSLSEWSGAPMKTPTLAGTRPGGAISAAWAVMNVLGISGYREKQGLVCQTRERI
EAGVNALGFEVLGKPLLGLVAFRHPQADTLALYSAMRQRGWFTSFTVEPPSLHLMLSPKHAEVADDYLADLAASLEAVKA
GDTAPKVEARYS

Specific function: Cleaves phosphorylated sphingoid bases (PSBs), such as sphingosine-1-phosphate, into fatty aldehydes and phosphoethanolamine. Possibly implicated in influencing the macrophage autophagy pathway [H]

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family. Sphingosine-1-phosphate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=400, Percent_Identity=36.75, Blast_Score=249, Evalue=2e-66,
Organism=Escherichia coli, GI1787769, Length=385, Percent_Identity=27.012987012987, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789934, Length=385, Percent_Identity=27.012987012987, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17543922, Length=372, Percent_Identity=36.8279569892473, Blast_Score=228, Evalue=5e-60,
Organism=Caenorhabditis elegans, GI17557272, Length=383, Percent_Identity=32.3759791122715, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI25148342, Length=370, Percent_Identity=32.972972972973, Blast_Score=206, Evalue=2e-53,
Organism=Saccharomyces cerevisiae, GI6320500, Length=408, Percent_Identity=33.3333333333333, Blast_Score=223, Evalue=3e-59,
Organism=Saccharomyces cerevisiae, GI6323905, Length=267, Percent_Identity=25.8426966292135, Blast_Score=74, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21355963, Length=377, Percent_Identity=36.8700265251989, Blast_Score=249, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24654344, Length=377, Percent_Identity=36.8700265251989, Blast_Score=249, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.2.27 [H]

Molecular weight: Translated: 44027; Mature: 44027

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQMPAEGRPWDEVRADMLARGAGDVAWRDGKTAVYVFNAGEDVHALQHEAYGLFMAENGL
CCCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCHHHHHHHHHEEEEEECCCC
GPLAFPSLAQMEKDVISMALGLLHGPEGSTGAITSGGTDSITMAIKTARDYARAKGMAKD
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEEEHHHHHHHHHCCCCCC
RHNIVIPRSGHLAFHKAALLMDIEIRSVPLKTDGSYEADPAAMAAAIDGATIMMVGSAPN
CCCEEEECCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCHHHHHHHCCCEEEEEEECCCC
FPHGIIDPIAELGKIAEEKDVWLHVDACVGGYFAPFARMNGVPVPDFDFAIPAVKSISAD
CCCHHHHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LHKYGYCAKGASTVLFRSVDLYKHMPFSLSEWSGAPMKTPTLAGTRPGGAISAAWAVMNV
HHHCCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LGISGYREKQGLVCQTRERIEAGVNALGFEVLGKPLLGLVAFRHPQADTLALYSAMRQRG
HHCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
WFTSFTVEPPSLHLMLSPKHAEVADDYLADLAASLEAVKAGDTAPKVEARYS
CEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MQMPAEGRPWDEVRADMLARGAGDVAWRDGKTAVYVFNAGEDVHALQHEAYGLFMAENGL
CCCCCCCCCHHHHHHHHHHCCCCCEEEECCCEEEEEEECCCHHHHHHHHHEEEEEECCCC
GPLAFPSLAQMEKDVISMALGLLHGPEGSTGAITSGGTDSITMAIKTARDYARAKGMAKD
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCEEEEEEHHHHHHHHHCCCCCC
RHNIVIPRSGHLAFHKAALLMDIEIRSVPLKTDGSYEADPAAMAAAIDGATIMMVGSAPN
CCCEEEECCCCCCEEEEEEEEEEEEEEEEECCCCCCCCCHHHHHHHCCCEEEEEEECCCC
FPHGIIDPIAELGKIAEEKDVWLHVDACVGGYFAPFARMNGVPVPDFDFAIPAVKSISAD
CCCHHHHHHHHHHHHCCCCCEEEEEEHHHCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
LHKYGYCAKGASTVLFRSVDLYKHMPFSLSEWSGAPMKTPTLAGTRPGGAISAAWAVMNV
HHHCCCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
LGISGYREKQGLVCQTRERIEAGVNALGFEVLGKPLLGLVAFRHPQADTLALYSAMRQRG
HHCCCCCCCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
WFTSFTVEPPSLHLMLSPKHAEVADDYLADLAASLEAVKAGDTAPKVEARYS
CEEEEEECCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA