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The map label for this gene is ccrA [H]

Identifier: 114798785

GI number: 114798785

Start: 3300244

End: 3301515

Strand: Direct

Name: ccrA [H]

Synonym: HNE_3118

Alternate gene names: 114798785

Gene position: 3300244-3301515 (Clockwise)

Preceding gene: 114797670

Following gene: 114798260

Centisome position: 89.07

GC content: 62.74

Gene sequence:

>1272_bases
ATGACCACTGAAACGGCAGGACGGGTTAAAAAAGACATCTACGACGTCGGCGAGATTCCGCCCGAATTCCATGTGCCCAA
GCTGATGCATGCCTGGTCGATCCGGCGCGAGCGCCATGGCCGCCCTATGAGCGCGATGCAGGTCGAGCAGGTGCCGGTGC
CCGAAATCGATTCCGATGAAGTGCTCGTCCTCGTGATGGCCGCCGGCGTCAATTACAATGGCATCTGGGCTGCGCTCGGC
GAGCCCGTGTCCCCCTTCGACATTCACAAACAGCCTTTCCATGTCGCAGGCTCTGACGCCGCCGGCATCGTCTGGGCGGT
CGGCCGCAAGGTCACCCGCGTGAAGCCGGGCGACGAGGTGGTCATCCACTGCAACCAGGACGACGGCGACGACGAAGAAT
GCAATGGCGGCGACCCCATGTTCTCGCCCAGCCAGCGCATCTGGGGCTATGAAACCCCCGATGGCTCCTTCGCGCAGTTC
TGCCGCGTCCAGGCCCGCCAGTGCATGCCGCGCCCCAAACACCTCACCTGGGAAGAGAGCGCCTGCTACACGCTGACCCT
GGCCACCGCCTACCGCATGCTGTTCGGCCACCGCCCGCACGTCCTCAAGCCCTCCGATAACGTCCTCGTCTGGGGCGCTT
CCGGCGGCCTTGGCAGCTTCGCTGTGCAGCTCTGCGCGATCGCGGGCGCCAATGCGATCGGCGTCGTTTCTTCTGAAGAC
AAGCGCGACTATGTGCTCTCGCTCGGCGCCAAAGGCGTCATCAACCGGAACGACTTCAACTGCTGGGGACAGCTGCCGAA
GGTCAACGGGCCGGAATTCAACGACTATATGAAAGAGAGCCGCAAGTTCGGCAAAGCCATCTGGGACATCACCGGCAAGA
AAGACGTCGACATCGTCTTTGAACACCCCGGCGAAGCGACCTTCCCGGTCTCGGTCTTCGTGGTGAAGCGCGGCGGCATG
GTGGTCATCTGCGCAGGCACCTCGGGCTTTAACCTCACCATGGATGCCCGCTTCCTCTGGATGCGGCAGAAGCGCGTGCA
GGGCTCCCACTTTGCAAACCTGAAACAGGCCTCTGAGGCCAACCAGCTCGTCCTCAACCGGCGCATAGACCCCGGCATGT
CGGAAGTCTTCACCTGGGCCGATATTCCGCTGGCGCACGAAAAGATGCTGGATAACAAGCACATGCCCGGCAACATGGCC
GTGCTCGTCACCTCTCCAAAGCCCGGCCTGCGCACGGTGGAAGACGTGCTCGCCATCAACGGCCAGGCTTGA

Upstream 100 bases:

>100_bases
GCGAAGCCTCACAACACTTGTCAGTGTGCCCGCGCCGGAGGGGGCTGTGAAGACGCCGTAAGCGCGCGCCTGCCCCCGGC
ACCAATACGGAGGAAGGTCC

Downstream 100 bases:

>100_bases
GGACCATGCTTTCCCGCGCCTTTGCCGAAGGCTTCGCGCGTGACTGGCTGGCCGCCTGGAACGACCGCGATATTGACCGC
ATCCTCTCTCACTACAGTGA

Product: crotonyl-CoA reductase

Products: NADP(+); Semiquinone. [C]

Alternate protein names: NA

Number of amino acids: Translated: 423; Mature: 422

Protein sequence:

>423_residues
MTTETAGRVKKDIYDVGEIPPEFHVPKLMHAWSIRRERHGRPMSAMQVEQVPVPEIDSDEVLVLVMAAGVNYNGIWAALG
EPVSPFDIHKQPFHVAGSDAAGIVWAVGRKVTRVKPGDEVVIHCNQDDGDDEECNGGDPMFSPSQRIWGYETPDGSFAQF
CRVQARQCMPRPKHLTWEESACYTLTLATAYRMLFGHRPHVLKPSDNVLVWGASGGLGSFAVQLCAIAGANAIGVVSSED
KRDYVLSLGAKGVINRNDFNCWGQLPKVNGPEFNDYMKESRKFGKAIWDITGKKDVDIVFEHPGEATFPVSVFVVKRGGM
VVICAGTSGFNLTMDARFLWMRQKRVQGSHFANLKQASEANQLVLNRRIDPGMSEVFTWADIPLAHEKMLDNKHMPGNMA
VLVTSPKPGLRTVEDVLAINGQA

Sequences:

>Translated_423_residues
MTTETAGRVKKDIYDVGEIPPEFHVPKLMHAWSIRRERHGRPMSAMQVEQVPVPEIDSDEVLVLVMAAGVNYNGIWAALG
EPVSPFDIHKQPFHVAGSDAAGIVWAVGRKVTRVKPGDEVVIHCNQDDGDDEECNGGDPMFSPSQRIWGYETPDGSFAQF
CRVQARQCMPRPKHLTWEESACYTLTLATAYRMLFGHRPHVLKPSDNVLVWGASGGLGSFAVQLCAIAGANAIGVVSSED
KRDYVLSLGAKGVINRNDFNCWGQLPKVNGPEFNDYMKESRKFGKAIWDITGKKDVDIVFEHPGEATFPVSVFVVKRGGM
VVICAGTSGFNLTMDARFLWMRQKRVQGSHFANLKQASEANQLVLNRRIDPGMSEVFTWADIPLAHEKMLDNKHMPGNMA
VLVTSPKPGLRTVEDVLAINGQA
>Mature_422_residues
TTETAGRVKKDIYDVGEIPPEFHVPKLMHAWSIRRERHGRPMSAMQVEQVPVPEIDSDEVLVLVMAAGVNYNGIWAALGE
PVSPFDIHKQPFHVAGSDAAGIVWAVGRKVTRVKPGDEVVIHCNQDDGDDEECNGGDPMFSPSQRIWGYETPDGSFAQFC
RVQARQCMPRPKHLTWEESACYTLTLATAYRMLFGHRPHVLKPSDNVLVWGASGGLGSFAVQLCAIAGANAIGVVSSEDK
RDYVLSLGAKGVINRNDFNCWGQLPKVNGPEFNDYMKESRKFGKAIWDITGKKDVDIVFEHPGEATFPVSVFVVKRGGMV
VICAGTSGFNLTMDARFLWMRQKRVQGSHFANLKQASEANQLVLNRRIDPGMSEVFTWADIPLAHEKMLDNKHMPGNMAV
LVTSPKPGLRTVEDVLAINGQA

Specific function: Unknown

COG id: COG0604

COG function: function code CR; NADPH:quinone reductase and related Zn-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the zinc-containing alcohol dehydrogenase family. Quinone oxidoreductase subfamily [H]

Homologues:

Organism=Homo sapiens, GI194239674, Length=387, Percent_Identity=23.2558139534884, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI13236495, Length=387, Percent_Identity=23.2558139534884, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI194239676, Length=276, Percent_Identity=27.536231884058, Blast_Score=70, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17507255, Length=390, Percent_Identity=26.4102564102564, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71988145, Length=237, Percent_Identity=29.1139240506329, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319520, Length=287, Percent_Identity=26.8292682926829, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6324486, Length=357, Percent_Identity=26.0504201680672, Blast_Score=77, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6323729, Length=211, Percent_Identity=31.7535545023697, Blast_Score=73, Evalue=7e-14,
Organism=Saccharomyces cerevisiae, GI6323961, Length=345, Percent_Identity=25.5072463768116, Blast_Score=71, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319621, Length=214, Percent_Identity=30.3738317757009, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013149
- InterPro:   IPR013154
- InterPro:   IPR002085
- InterPro:   IPR011032
- InterPro:   IPR016040 [H]

Pfam domain/function: PF08240 ADH_N; PF00107 ADH_zinc_N [H]

EC number: 1.6.5.5 [C]

Molecular weight: Translated: 46685; Mature: 46554

Theoretical pI: Translated: 6.83; Mature: 6.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTETAGRVKKDIYDVGEIPPEFHVPKLMHAWSIRRERHGRPMSAMQVEQVPVPEIDSDE
CCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCC
VLVLVMAAGVNYNGIWAALGEPVSPFDIHKQPFHVAGSDAAGIVWAVGRKVTRVKPGDEV
EEEEEEECCCCCCCEEHCCCCCCCCCCCCCCCCEECCCCCCCEEEECCCEEEEECCCCEE
VIHCNQDDGDDEECNGGDPMFSPSQRIWGYETPDGSFAQFCRVQARQCMPRPKHLTWEES
EEEECCCCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCC
ACYTLTLATAYRMLFGHRPHVLKPSDNVLVWGASGGLGSFAVQLCAIAGANAIGVVSSED
CEEEEEHHHHHHHHHCCCCCEECCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCC
KRDYVLSLGAKGVINRNDFNCWGQLPKVNGPEFNDYMKESRKFGKAIWDITGKKDVDIVF
CCCEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCEEEEE
EHPGEATFPVSVFVVKRGGMVVICAGTSGFNLTMDARFLWMRQKRVQGSHFANLKQASEA
ECCCCCCCCEEEEEEECCCEEEEEECCCCCEEEECHHEEEHHHHHCCCCHHHHHHHHHHH
NQLVLNRRIDPGMSEVFTWADIPLAHEKMLDNKHMPGNMAVLVTSPKPGLRTVEDVLAIN
HHEEEECCCCCCHHHHEECCCCCHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCC
GQA
CCC
>Mature Secondary Structure 
TTETAGRVKKDIYDVGEIPPEFHVPKLMHAWSIRRERHGRPMSAMQVEQVPVPEIDSDE
CCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCCC
VLVLVMAAGVNYNGIWAALGEPVSPFDIHKQPFHVAGSDAAGIVWAVGRKVTRVKPGDEV
EEEEEEECCCCCCCEEHCCCCCCCCCCCCCCCCEECCCCCCCEEEECCCEEEEECCCCEE
VIHCNQDDGDDEECNGGDPMFSPSQRIWGYETPDGSFAQFCRVQARQCMPRPKHLTWEES
EEEECCCCCCCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHCCCCCCCCCCCC
ACYTLTLATAYRMLFGHRPHVLKPSDNVLVWGASGGLGSFAVQLCAIAGANAIGVVSSED
CEEEEEHHHHHHHHHCCCCCEECCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCC
KRDYVLSLGAKGVINRNDFNCWGQLPKVNGPEFNDYMKESRKFGKAIWDITGKKDVDIVF
CCCEEEECCCCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHEECCCCCCEEEEE
EHPGEATFPVSVFVVKRGGMVVICAGTSGFNLTMDARFLWMRQKRVQGSHFANLKQASEA
ECCCCCCCCEEEEEEECCCEEEEEECCCCCEEEECHHEEEHHHHHCCCCHHHHHHHHHHH
NQLVLNRRIDPGMSEVFTWADIPLAHEKMLDNKHMPGNMAVLVTSPKPGLRTVEDVLAIN
HHEEEECCCCCCHHHHEECCCCCHHHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHCC
GQA
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.017 {9,10-phenanthrenequinone}} 0.0153 {2,6-dichlorophenolindophenol}} 0.013 {2,6-dichlorophenolindophenol}} 0.007 {NADPH}} 0.0069 {NADPH}} 0.0024 {1,2-naphthoquinone}} 0.26 {1,4-naphthoquinone}} 0.065 {1,4-benzoquinone}} [C]

Substrates: NADPH; Quinone [C]

Specific reaction: NADPH + Quinone = NADP(+) + Semiquinone. [C]

General reaction: Oxidation; Reduction [C]

Inhibitor: 2, 3-Dimercaptopropanol; 2, 5-Dichloro-3, 6-dihydroxy -1, 4-benzoquinone; 4-Hydroxy coumarin; 5, 5'-Dithiobis (2-nitrobenzoate); ADP; Cibacronblue3GA; Coumarin; Cu2+; Dicoumarol warfarin, coumarin; Dithiothreitol preincubation with 9, 10-phenanthrenequino

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]