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The map label for this gene is ccrM [H]

Identifier: 114798609

GI number: 114798609

Start: 1968462

End: 1969544

Strand: Reverse

Name: ccrM [H]

Synonym: HNE_1901

Alternate gene names: 114798609

Gene position: 1969544-1968462 (Counterclockwise)

Preceding gene: 114798617

Following gene: 114800225

Centisome position: 53.16

GC content: 61.31

Gene sequence:

>1083_bases
ATGACCATTCAGCGTAACACGATCATCCAGGGCGATTGCATCGAGGTCCTGTCCCGTATTCCGGACAAGTCCGTCGATCT
CGTCTTTGCCGATCCCCCGTATAACCTTCAGCTCGGCGGCGGGCTGACGCGCCCGGATCAGTCCGTGGTAGACGGTGTTG
ACGACGAATGGGACAAATTCGCCAGCTTTGACGACTATGATCTGTTTACGCATCAATGGCTGGAAGAATGCCGCCGTGTC
CTGAAGGATGACGGCGCCATCTGGGTGATCGGCTCCTATCACAATATTTTCCGTGTCGGGACGGTTCTTCAGGATCAGGG
CTTCTGGATTCAGAATGACGTGATCTGGCTGAAATCAAACCCGATGCCGAATTTCAAGGGCACGCGCTTCCAGAACGCGC
ATGAAACGCTGATCTGGGCGGGCAAGTCGAAGGACAGCCGCGTTACCTTTAACTATGACGCGCTAAAAACCTTCAATGAA
GACAAACAGATGCGGTCTGACTGGACGATCCCCCTCTGCACCGGTGGCGAGCGTCTGAAAGATGAGGCCGGCCGCAAGGC
GCATCCGACCCAGAAACCGGAATCCCTGCTTCACCGCGTCCTGCTTGCCACGTCCAATCCGCGCGATCTCGTCCTTGATC
CGTTCTCCGGCACCGGCACGACAGCCGCTGCTGCCCGCCGCCTCGGCCGCGATTTCATCGGCATCGAGCGCGAAGAGGGC
TACGCCCGCCTCTCGCGGGCCCGCATCAACGCGATCACGCCGCTGGAAGGCGAAGTGCTGGAAACAGAGCGCAGCAAGAA
ATCACTCGCCCGCGTGCCGTTCGGCGCCCTGATCGAAACCGGCTGGCTGAAGCCCGGCGACCGCCTCTTCTCCGCCCAGC
GCCGCCACCAGGCCCGCATCCGCGTCGATGGATCGCTCACCACAGGCGCCATCACCGGCTCGATCCACCGCCTCGGGGCG
CAGGTCCAGCAGGCCCCCGCCTGCAATGGCTGGACTTACTGGCACTACGAAACCGACAAGCGCGACCTTGCGCCGATCGA
TCTTCTGCGCCGCCGTTACCGTGAAGAGATGGGCCTCGCCTGA

Upstream 100 bases:

>100_bases
CGCTGGTAACCTTTCGGCAGGGTTTCCTTCACTGAGTAGTAACCGGCGCGATTCAGGGTCGTCTGCAACACTCACAGTAG
AGTCGCAGGGCGAACGATCC

Downstream 100 bases:

>100_bases
GACCTTCCGTTCACGAATAGCTTGTGAGAATCTCGTCCAGGGCGCGCTCAAGATCCTTGATCGTCGCCACATGGCTCATC
TGTTTGCAATAGGGCGCATA

Product: modification methylase CcrM

Products: NA

Alternate protein names: M.CcrMI; Adenine-specific methyltransferase CcrMI [H]

Number of amino acids: Translated: 360; Mature: 359

Protein sequence:

>360_residues
MTIQRNTIIQGDCIEVLSRIPDKSVDLVFADPPYNLQLGGGLTRPDQSVVDGVDDEWDKFASFDDYDLFTHQWLEECRRV
LKDDGAIWVIGSYHNIFRVGTVLQDQGFWIQNDVIWLKSNPMPNFKGTRFQNAHETLIWAGKSKDSRVTFNYDALKTFNE
DKQMRSDWTIPLCTGGERLKDEAGRKAHPTQKPESLLHRVLLATSNPRDLVLDPFSGTGTTAAAARRLGRDFIGIEREEG
YARLSRARINAITPLEGEVLETERSKKSLARVPFGALIETGWLKPGDRLFSAQRRHQARIRVDGSLTTGAITGSIHRLGA
QVQQAPACNGWTYWHYETDKRDLAPIDLLRRRYREEMGLA

Sequences:

>Translated_360_residues
MTIQRNTIIQGDCIEVLSRIPDKSVDLVFADPPYNLQLGGGLTRPDQSVVDGVDDEWDKFASFDDYDLFTHQWLEECRRV
LKDDGAIWVIGSYHNIFRVGTVLQDQGFWIQNDVIWLKSNPMPNFKGTRFQNAHETLIWAGKSKDSRVTFNYDALKTFNE
DKQMRSDWTIPLCTGGERLKDEAGRKAHPTQKPESLLHRVLLATSNPRDLVLDPFSGTGTTAAAARRLGRDFIGIEREEG
YARLSRARINAITPLEGEVLETERSKKSLARVPFGALIETGWLKPGDRLFSAQRRHQARIRVDGSLTTGAITGSIHRLGA
QVQQAPACNGWTYWHYETDKRDLAPIDLLRRRYREEMGLA
>Mature_359_residues
TIQRNTIIQGDCIEVLSRIPDKSVDLVFADPPYNLQLGGGLTRPDQSVVDGVDDEWDKFASFDDYDLFTHQWLEECRRVL
KDDGAIWVIGSYHNIFRVGTVLQDQGFWIQNDVIWLKSNPMPNFKGTRFQNAHETLIWAGKSKDSRVTFNYDALKTFNED
KQMRSDWTIPLCTGGERLKDEAGRKAHPTQKPESLLHRVLLATSNPRDLVLDPFSGTGTTAAAARRLGRDFIGIEREEGY
ARLSRARINAITPLEGEVLETERSKKSLARVPFGALIETGWLKPGDRLFSAQRRHQARIRVDGSLTTGAITGSIHRLGAQ
VQQAPACNGWTYWHYETDKRDLAPIDLLRRRYREEMGLA

Specific function: This methylase recognizes the double-stranded sequence GANTC and causes specific methylation on A-2 on both strands. Ccrm-mediated methylation has important cellular functions. Appears to contribute to the accurate cell-cycle control of DNA replication an

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=288, Percent_Identity=31.25, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 40884; Mature: 40752

Theoretical pI: Translated: 7.61; Mature: 7.61

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIQRNTIIQGDCIEVLSRIPDKSVDLVFADPPYNLQLGGGLTRPDQSVVDGVDDEWDKF
CCCCCCCEECCHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCHHHHCCCCHHHHHH
ASFDDYDLFTHQWLEECRRVLKDDGAIWVIGSYHNIFRVGTVLQDQGFWIQNDVIWLKSN
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEECCCCEEECCEEEEECC
PMPNFKGTRFQNAHETLIWAGKSKDSRVTFNYDALKTFNEDKQMRSDWTIPLCTGGERLK
CCCCCCCCCCCCCCCEEEEECCCCCCEEEEEHHHHHHCCHHHHHHCCCCEEEECCCHHHH
DEAGRKAHPTQKPESLLHRVLLATSNPRDLVLDPFSGTGTTAAAARRLGRDFIGIEREEG
HHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCHHHCCCCCHHH
YARLSRARINAITPLEGEVLETERSKKSLARVPFGALIETGWLKPGDRLFSAQRRHQARI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHCCEEE
RVDGSLTTGAITGSIHRLGAQVQQAPACNGWTYWHYETDKRDLAPIDLLRRRYREEMGLA
EECCCEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TIQRNTIIQGDCIEVLSRIPDKSVDLVFADPPYNLQLGGGLTRPDQSVVDGVDDEWDKF
CCCCCCEECCHHHHHHHHCCCCCEEEEECCCCCEEEECCCCCCCCHHHHCCCCHHHHHH
ASFDDYDLFTHQWLEECRRVLKDDGAIWVIGSYHNIFRVGTVLQDQGFWIQNDVIWLKSN
CCCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCHHHHHHEEECCCCEEECCEEEEECC
PMPNFKGTRFQNAHETLIWAGKSKDSRVTFNYDALKTFNEDKQMRSDWTIPLCTGGERLK
CCCCCCCCCCCCCCCEEEEECCCCCCEEEEEHHHHHHCCHHHHHHCCCCEEEECCCHHHH
DEAGRKAHPTQKPESLLHRVLLATSNPRDLVLDPFSGTGTTAAAARRLGRDFIGIEREEG
HHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCHHHHHHHHHCHHHCCCCCHHH
YARLSRARINAITPLEGEVLETERSKKSLARVPFGALIETGWLKPGDRLFSAQRRHQARI
HHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCCCCCCHHHHHHHHHCCEEE
RVDGSLTTGAITGSIHRLGAQVQQAPACNGWTYWHYETDKRDLAPIDLLRRRYREEMGLA
EECCCEECCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8289276 [H]