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The map label for this gene is trmH [C]

Identifier: 114798567

GI number: 114798567

Start: 3479201

End: 3479734

Strand: Direct

Name: trmH [C]

Synonym: HNE_3326

Alternate gene names: 114798567

Gene position: 3479201-3479734 (Clockwise)

Preceding gene: 114797073

Following gene: 114799447

Centisome position: 93.91

GC content: 62.17

Gene sequence:

>534_bases
ATGCGGGGCTATTTTGCGATTGGTTCTGAACGCATTTCCAAGCCGATGAACCTCGGCGCCCTGATGCGTACCGCCCATGC
CTTCGGGGCGAGCTTCGTATTCTCAATTGATGCCGAGCGTAACCTGAAAGAAGCCTACGCCGCCGACACGTCAAAGTCCG
CCGGACAGATCCCCTATTATCAATGGGACTCGCTGGACGACATGCAGCTGCCCAAAGGCTGCCAGCTCGTCGGCATCGAA
CTGACAGACGACGCTGTCGAGCTGCCCGTGTTCCGCCATCCTCCGCAAGCGGCCTATATTCTGGGGCCGGAAAAGGGAAA
CCTGTCGGCCGAGGCCGTCGCGCGCTGCGCCCATGTCGTGAAAATCCCGACGCGGTTCTGCATCAATGTCAGCCTGGCGG
GCGCGCTGGTGATGTATGACCGGCATCTCTCAATGGGGGGATATGGCCCCCGCCCCATCATGCCGGGCGGGCCGGATGGC
GCGCGCCGCCGCAAGCATGTTCCGGGACCGGACAGACAGAAGCCTGAAGGGTGA

Upstream 100 bases:

>100_bases
CCGTTGGCCAGCTGAAACTCGCCCGCCGCGTTTCCCTGGGCGGCCCGCTTGCCTTGTTGATCGTTGTGGCGATCGGCCGC
TTCTGGCTGGGGGCGTTCTG

Downstream 100 bases:

>100_bases
ACGGCCGAACTTCTGAAAACTTCAGTGCCCGCCGAGAAGTCTCTGGACCATGCCCAGCATCCCGGTAAAGCGCAGGCCGC
CATCGCGCACGGCAAGCGCG

Product: RNA methyltransferase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: RNA Methyltransferase; 23S RRNA Methyltransferase; RNA Methyltransferase TrmH Family; Cold-Shock RNA Methyltransferase; rRNA Methylase; TrmH Family RNA Methyltransferase; TRNA/RRNA MethyltransferaU; rRNA Methyltransferase; SpoU RRNA Methylase Family Protein; rRNA Methylase-Family Protein; SpoU Class RRNA Methylase

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MRGYFAIGSERISKPMNLGALMRTAHAFGASFVFSIDAERNLKEAYAADTSKSAGQIPYYQWDSLDDMQLPKGCQLVGIE
LTDDAVELPVFRHPPQAAYILGPEKGNLSAEAVARCAHVVKIPTRFCINVSLAGALVMYDRHLSMGGYGPRPIMPGGPDG
ARRRKHVPGPDRQKPEG

Sequences:

>Translated_177_residues
MRGYFAIGSERISKPMNLGALMRTAHAFGASFVFSIDAERNLKEAYAADTSKSAGQIPYYQWDSLDDMQLPKGCQLVGIE
LTDDAVELPVFRHPPQAAYILGPEKGNLSAEAVARCAHVVKIPTRFCINVSLAGALVMYDRHLSMGGYGPRPIMPGGPDG
ARRRKHVPGPDRQKPEG
>Mature_177_residues
MRGYFAIGSERISKPMNLGALMRTAHAFGASFVFSIDAERNLKEAYAADTSKSAGQIPYYQWDSLDDMQLPKGCQLVGIE
LTDDAVELPVFRHPPQAAYILGPEKGNLSAEAVARCAHVVKIPTRFCINVSLAGALVMYDRHLSMGGYGPRPIMPGGPDG
ARRRKHVPGPDRQKPEG

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 19269; Mature: 19269

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGYFAIGSERISKPMNLGALMRTAHAFGASFVFSIDAERNLKEAYAADTSKSAGQIPYY
CCCEEECCHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCE
QWDSLDDMQLPKGCQLVGIELTDDAVELPVFRHPPQAAYILGPEKGNLSAEAVARCAHVV
ECCCCCCCCCCCCCEEEEEEECCCCEECCEEECCCCEEEEECCCCCCCCHHHHHHHHHHH
KIPTRFCINVSLAGALVMYDRHLSMGGYGPRPIMPGGPDGARRRKHVPGPDRQKPEG
CCCCCEEEEEEHHCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MRGYFAIGSERISKPMNLGALMRTAHAFGASFVFSIDAERNLKEAYAADTSKSAGQIPYY
CCCEEECCHHHHCCCCCHHHHHHHHHHCCCEEEEEECCCCCHHHHHHCCCCCCCCCCCCE
QWDSLDDMQLPKGCQLVGIELTDDAVELPVFRHPPQAAYILGPEKGNLSAEAVARCAHVV
ECCCCCCCCCCCCCEEEEEEECCCCEECCEEECCCCEEEEECCCCCCCCHHHHHHHHHHH
KIPTRFCINVSLAGALVMYDRHLSMGGYGPRPIMPGGPDGARRRKHVPGPDRQKPEG
CCCCCEEEEEEHHCEEEEEECCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA