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The map label for this gene is yngF [H]

Identifier: 114798522

GI number: 114798522

Start: 52868

End: 53689

Strand: Direct

Name: yngF [H]

Synonym: HNE_0065

Alternate gene names: 114798522

Gene position: 52868-53689 (Clockwise)

Preceding gene: 114798190

Following gene: 114798515

Centisome position: 1.43

GC content: 63.38

Gene sequence:

>822_bases
ATGAACGACCGCGTTTTGATCACGATGCTCGAAGACGGCATTGCCGATGTGCGCCTTAACCGCACCGACAAGATGAATGC
CCTCGACCCCGCCATGTTTGCCGGCATTGCCGAGGCCATCGACAAGCTGAAAGCCATGAAGGGCCTGCGCGTCGTGGTGC
TCTCCGGCGAGGGCCGCGCCTTCTGCGCCGGGCTGGACCTGTCGAATTTCACCGGCGGGGGCGGCGGCAGCGGCGAGAAG
AAACCGGAAAAAGCGAGCCCCACCAGCAATCTGGATGGGCGCAGCCATGGCATCGCAAACCTTGCCCAATATGTGGCCTG
GGGCTGGCGCGAGCTGAACGTGCCGGTGATCGCGGCCGCCCATGGCGTGGCATTCGGCGGCGGCTTTCAGATCCTTTCGG
GCGCTGACATCCGCTTCATCCATCCTGAGACGCGCTGCGCCATTATGGAGATGAAATGGGGCCTCGTGCCGGATATGGCC
GGTTACCCCCTCTGGCGCGGCAATGTGCGCGACGATGTGCTCCGTGAGCTGACCTACACCAATCGCGAGTTCCGCGGCAC
GGAGGCCCAGGCACTGGGCTTTGCCACGCATGTGTCTGAGAACCCGCTGGAAGATGCGCTCGCGCTCGCCCGCGTGATGG
CAGCGAAACACCCCGCCGCGATGCAGGGGGCAAAGCGCCTCTTCAACGCGATGCAGGATGCCACCGACGCCGAGCTTCTC
CAGATGGAAAGCGACGAGCAGAACAAGGTGATGCGCACGCCCAACCAGATGGAAGCGGTGATGTCGGAAATGCAGAAGCG
CAAACCGGTGTTCGCGGAGTAA

Upstream 100 bases:

>100_bases
GAAGAGGCGAGGCGGAGACAACCTTTCGCGCTGGGGCAGTCCTTGACGTCCCCAGCGGAAGGGCGCCAGTTTCATCTCAA
AGTTCCGGAGGACGACCCAG

Downstream 100 bases:

>100_bases
TTCTTCAAGCCCCCTCGCTGGAAGCGGGGAGGGGGCTAGACCGTCCGCACCGGCGCGCCGGCCTTGGCCAGCGCAGCCCG
CGCCTCTGCCAAACTCGCCT

Product: enoyl-CoA hydratase

Products: trans-2-enoyl-CoA; trans-3-enoyl-CoA; H2O

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MNDRVLITMLEDGIADVRLNRTDKMNALDPAMFAGIAEAIDKLKAMKGLRVVVLSGEGRAFCAGLDLSNFTGGGGGSGEK
KPEKASPTSNLDGRSHGIANLAQYVAWGWRELNVPVIAAAHGVAFGGGFQILSGADIRFIHPETRCAIMEMKWGLVPDMA
GYPLWRGNVRDDVLRELTYTNREFRGTEAQALGFATHVSENPLEDALALARVMAAKHPAAMQGAKRLFNAMQDATDAELL
QMESDEQNKVMRTPNQMEAVMSEMQKRKPVFAE

Sequences:

>Translated_273_residues
MNDRVLITMLEDGIADVRLNRTDKMNALDPAMFAGIAEAIDKLKAMKGLRVVVLSGEGRAFCAGLDLSNFTGGGGGSGEK
KPEKASPTSNLDGRSHGIANLAQYVAWGWRELNVPVIAAAHGVAFGGGFQILSGADIRFIHPETRCAIMEMKWGLVPDMA
GYPLWRGNVRDDVLRELTYTNREFRGTEAQALGFATHVSENPLEDALALARVMAAKHPAAMQGAKRLFNAMQDATDAELL
QMESDEQNKVMRTPNQMEAVMSEMQKRKPVFAE
>Mature_273_residues
MNDRVLITMLEDGIADVRLNRTDKMNALDPAMFAGIAEAIDKLKAMKGLRVVVLSGEGRAFCAGLDLSNFTGGGGGSGEK
KPEKASPTSNLDGRSHGIANLAQYVAWGWRELNVPVIAAAHGVAFGGGFQILSGADIRFIHPETRCAIMEMKWGLVPDMA
GYPLWRGNVRDDVLRELTYTNREFRGTEAQALGFATHVSENPLEDALALARVMAAKHPAAMQGAKRLFNAMQDATDAELL
QMESDEQNKVMRTPNQMEAVMSEMQKRKPVFAE

Specific function: Could Possibly Oxidizes Fatty Acids Using Specific Components (By Similarity). [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI70995211, Length=268, Percent_Identity=26.865671641791, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI4502327, Length=280, Percent_Identity=26.7857142857143, Blast_Score=71, Evalue=8e-13,
Organism=Escherichia coli, GI1787659, Length=268, Percent_Identity=25.7462686567164, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI221142681, Length=265, Percent_Identity=24.9056603773585, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787660, Length=272, Percent_Identity=26.4705882352941, Blast_Score=64, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17534483, Length=271, Percent_Identity=26.9372693726937, Blast_Score=94, Evalue=9e-20,
Organism=Caenorhabditis elegans, GI17540714, Length=243, Percent_Identity=29.2181069958848, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17554946, Length=261, Percent_Identity=24.5210727969349, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24653139, Length=267, Percent_Identity=28.4644194756554, Blast_Score=89, Evalue=3e-18,
Organism=Drosophila melanogaster, GI19920382, Length=267, Percent_Identity=26.5917602996255, Blast_Score=89, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: 4.2.1.17

Molecular weight: Translated: 29750; Mature: 29750

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
5.9 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDRVLITMLEDGIADVRLNRTDKMNALDPAMFAGIAEAIDKLKAMKGLRVVVLSGEGRA
CCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCE
FCAGLDLSNFTGGGGGSGEKKPEKASPTSNLDGRSHGIANLAQYVAWGWRELNVPVIAAA
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEECCCCEEEEC
HGVAFGGGFQILSGADIRFIHPETRCAIMEMKWGLVPDMAGYPLWRGNVRDDVLRELTYT
CCEEECCCEEEECCCCEEEECCCHHEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
NREFRGTEAQALGFATHVSENPLEDALALARVMAAKHPAAMQGAKRLFNAMQDATDAELL
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHH
QMESDEQNKVMRTPNQMEAVMSEMQKRKPVFAE
HHCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNDRVLITMLEDGIADVRLNRTDKMNALDPAMFAGIAEAIDKLKAMKGLRVVVLSGEGRA
CCCCEEEEEECCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCE
FCAGLDLSNFTGGGGGSGEKKPEKASPTSNLDGRSHGIANLAQYVAWGWRELNVPVIAAA
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCEECCCCEEEEC
HGVAFGGGFQILSGADIRFIHPETRCAIMEMKWGLVPDMAGYPLWRGNVRDDVLRELTYT
CCEEECCCEEEECCCCEEEECCCHHEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHC
NREFRGTEAQALGFATHVSENPLEDALALARVMAAKHPAAMQGAKRLFNAMQDATDAELL
CCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHH
QMESDEQNKVMRTPNQMEAVMSEMQKRKPVFAE
HHCCCCCHHHHCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.05 {crotonyl-CoA}} 0.008 {2-decenoyl-CoA}} [C]

Substrates: (3S)-3-hydroxyacyl-CoA

Specific reaction: (3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H2O

General reaction: addition (of H2O to carbon carbon double bondelimination (of H2O C-O bond cleavagehydration [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9387222; 9384377 [H]