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The map label for this gene is holB [H]

Identifier: 114798506

GI number: 114798506

Start: 1858649

End: 1859584

Strand: Reverse

Name: holB [H]

Synonym: HNE_1812

Alternate gene names: 114798506

Gene position: 1859584-1858649 (Counterclockwise)

Preceding gene: 114799318

Following gene: 114798238

Centisome position: 50.19

GC content: 65.38

Gene sequence:

>936_bases
ATGAGCCCCGCGCTGCCTCTGTTCGGGCATGAGGCGGCCGAGGCCGCGTTCCTTTCGGCGCATCGCAGTGGCCGATTGCA
CCATGCCTGGCTGATCGAAGGTCCGTCAGGCGTTGGCAAAGCGCGGGTTGCCGAGCGCATTGCGGCGTATCTGCTGGGCG
CGCGTGGCCCGGCGGCGGCGCCTTTTGATGCGCCTGCTGACGACCCGGTTATGCGCGCCATTCTTGCCGGAAATCATCCT
GACATGAATGTCCAGCGCCGCGAGCTCAATGATAAGGGCAAACTTACGCAGGACATAACCGTTGAGCAGATCCGCCAGGT
GGGTCACTTCTTTTCGATGAAACCGGCAATGGGCGGCTGGCGCGTCGGGATTGTTGACGCCATTGATGAGGCAAACCGCA
ACTCGGCAAACGCCTTGCTCAAGACCCTTGAGGAACCCCCGGCGCAAAGCATCCTGTTGCTGGTCAATCATCGATCCAGA
CCGATCCTGCCGACAATCCGGTCCCGCTGCCGCGTGCTGCAGCTTTCGGTGCTGTCTGCCGAAGACTGTGAGCGCGCCCT
GGAAGCAGCGGGCGCGCCGCGCGAAGCGAGCGCATTGGCGAGGGGGCGACCCGGACTGGGGATGAGGCTGTCATCCGGCG
CCGCGATGAGCGCATCCAGCGCGGCGCGCGCGCTGATAAAGGCCATGCCGCGGCCGCCGGACAAGCTTGTTTCTGCGGCG
CTGAGCGCGGCCAGCGCGGGCCCGGAGGCTGAACTTGCATTCCGTGACGAGGTGCTGGACTGGGTTGCCGCGCGGGCCGA
GACAGAGCCGAAGGCGGCCAAGGCGTGGCTGGGACTGGCGCGCCTTTCTGGCGATGCGGAGACCTTGAACATGGACGCCG
CCCAAGTGGCCTCCAAGATAATCGCGGCGATGTATGACGCCGCCCCAGCCAAGTGA

Upstream 100 bases:

>100_bases
GGTGCCGGTTGATCGATACGGATGTGACGCCTGATCAGGTGCTCGATGCCGCGTGGAAACAGGTCGCCACGCTGTTGCAG
AACGCGAGGACCGCCGCGCC

Downstream 100 bases:

>100_bases
ATGATCTTTGATGTTTGATACGCATGTAAACCTGCATGGCGAAGCTTTTGCAGACGATCTGCAGGACGTTCTGGGCCGGG
CGCGCGAAGCGGGCGTGCGG

Product: putative DNA polymerase III, delta prime subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSPALPLFGHEAAEAAFLSAHRSGRLHHAWLIEGPSGVGKARVAERIAAYLLGARGPAAAPFDAPADDPVMRAILAGNHP
DMNVQRRELNDKGKLTQDITVEQIRQVGHFFSMKPAMGGWRVGIVDAIDEANRNSANALLKTLEEPPAQSILLLVNHRSR
PILPTIRSRCRVLQLSVLSAEDCERALEAAGAPREASALARGRPGLGMRLSSGAAMSASSAARALIKAMPRPPDKLVSAA
LSAASAGPEAELAFRDEVLDWVAARAETEPKAAKAWLGLARLSGDAETLNMDAAQVASKIIAAMYDAAPAK

Sequences:

>Translated_311_residues
MSPALPLFGHEAAEAAFLSAHRSGRLHHAWLIEGPSGVGKARVAERIAAYLLGARGPAAAPFDAPADDPVMRAILAGNHP
DMNVQRRELNDKGKLTQDITVEQIRQVGHFFSMKPAMGGWRVGIVDAIDEANRNSANALLKTLEEPPAQSILLLVNHRSR
PILPTIRSRCRVLQLSVLSAEDCERALEAAGAPREASALARGRPGLGMRLSSGAAMSASSAARALIKAMPRPPDKLVSAA
LSAASAGPEAELAFRDEVLDWVAARAETEPKAAKAWLGLARLSGDAETLNMDAAQVASKIIAAMYDAAPAK
>Mature_310_residues
SPALPLFGHEAAEAAFLSAHRSGRLHHAWLIEGPSGVGKARVAERIAAYLLGARGPAAAPFDAPADDPVMRAILAGNHPD
MNVQRRELNDKGKLTQDITVEQIRQVGHFFSMKPAMGGWRVGIVDAIDEANRNSANALLKTLEEPPAQSILLLVNHRSRP
ILPTIRSRCRVLQLSVLSAEDCERALEAAGAPREASALARGRPGLGMRLSSGAAMSASSAARALIKAMPRPPDKLVSAAL
SAASAGPEAELAFRDEVLDWVAARAETEPKAAKAWLGLARLSGDAETLNMDAAQVASKIIAAMYDAAPAK

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786676, Length=206, Percent_Identity=32.0388349514563, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003959
- InterPro:   IPR004622 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 32841; Mature: 32710

Theoretical pI: Translated: 9.22; Mature: 9.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPALPLFGHEAAEAAFLSAHRSGRLHHAWLIEGPSGVGKARVAERIAAYLLGARGPAAA
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCC
PFDAPADDPVMRAILAGNHPDMNVQRRELNDKGKLTQDITVEQIRQVGHFFSMKPAMGGW
CCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
RVGIVDAIDEANRNSANALLKTLEEPPAQSILLLVNHRSRPILPTIRSRCRVLQLSVLSA
EEHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCH
EDCERALEAAGAPREASALARGRPGLGMRLSSGAAMSASSAARALIKAMPRPPDKLVSAA
HHHHHHHHHCCCCHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
LSAASAGPEAELAFRDEVLDWVAARAETEPKAAKAWLGLARLSGDAETLNMDAAQVASKI
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHH
IAAMYDAAPAK
HHHHHHCCCCC
>Mature Secondary Structure 
SPALPLFGHEAAEAAFLSAHRSGRLHHAWLIEGPSGVGKARVAERIAAYLLGARGPAAA
CCCCCCCCCHHHHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCC
PFDAPADDPVMRAILAGNHPDMNVQRRELNDKGKLTQDITVEQIRQVGHFFSMKPAMGGW
CCCCCCCCHHHHHHHHCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCC
RVGIVDAIDEANRNSANALLKTLEEPPAQSILLLVNHRSRPILPTIRSRCRVLQLSVLSA
EEHHHHHHHHCCCCHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHCH
EDCERALEAAGAPREASALARGRPGLGMRLSSGAAMSASSAARALIKAMPRPPDKLVSAA
HHHHHHHHHCCCCHHHHHHHCCCCCCCEEECCCCCCCHHHHHHHHHHHCCCCHHHHHHHH
LSAASAGPEAELAFRDEVLDWVAARAETEPKAAKAWLGLARLSGDAETLNMDAAQVASKI
HHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHCCCCHHHHHHHH
IAAMYDAAPAK
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8550441; 10984043 [H]