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The map label for this gene is thiS [H]

Identifier: 114798481

GI number: 114798481

Start: 2350028

End: 2350240

Strand: Reverse

Name: thiS [H]

Synonym: HNE_2247

Alternate gene names: 114798481

Gene position: 2350240-2350028 (Counterclockwise)

Preceding gene: 114799292

Following gene: 114799032

Centisome position: 63.43

GC content: 63.85

Gene sequence:

>213_bases
ATGCAGATCACACTGAACGGAGCGGCGCTCGAGATCGCCCCCGGCACGACGATTGCGCAGCTTATCCACCGGATTGCCGG
CGAGACAGGGCGTGACCCGCGCAGCGTCGCGGTTGAGCGCAATCTCGAGATCGTGCCGAAGTCCGAGCATGCCATGACGG
TGCTGACCGAGGGGGACCGGCTGGAAATCGTGCAATTCGTGGGTGGGGGATAA

Upstream 100 bases:

>100_bases
GGTTCGGCCCGCCGAGGACATATACCGGTTTCGACATGCGCCTTCGTTTGCGCTGTAACGGCCAGCGTGGCAAGAGGGCA
ACAGTCAGGGAATTTTGGTG

Downstream 100 bases:

>100_bases
GGGGCGAAAAAACTCCAAAAAAACTCCAACGATTCCTCCAACGAGGGGCCGAAATCCTCCAAGAAAACTCCAACCACGCA
GGCCTTGCGGCCGTTTTTCA

Product: thiamine biosynthesis protein ThiS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 70; Mature: 70

Protein sequence:

>70_residues
MQITLNGAALEIAPGTTIAQLIHRIAGETGRDPRSVAVERNLEIVPKSEHAMTVLTEGDRLEIVQFVGGG

Sequences:

>Translated_70_residues
MQITLNGAALEIAPGTTIAQLIHRIAGETGRDPRSVAVERNLEIVPKSEHAMTVLTEGDRLEIVQFVGGG
>Mature_70_residues
MQITLNGAALEIAPGTTIAQLIHRIAGETGRDPRSVAVERNLEIVPKSEHAMTVLTEGDRLEIVQFVGGG

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S [H]

COG id: COG2104

COG function: function code H; Sulfur transfer protein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR012675
- InterPro:   IPR016155
- InterPro:   IPR010035
- InterPro:   IPR008867
- InterPro:   IPR003749 [H]

Pfam domain/function: PF05690 ThiG; PF02597 ThiS [H]

EC number: NA

Molecular weight: Translated: 7450; Mature: 7450

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQITLNGAALEIAPGTTIAQLIHRIAGETGRDPRSVAVERNLEIVPKSEHAMTVLTEGDR
CEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCEEEEECCEEEEECCCCEEEEEECCCE
LEIVQFVGGG
EEEEEEECCC
>Mature Secondary Structure
MQITLNGAALEIAPGTTIAQLIHRIAGETGRDPRSVAVERNLEIVPKSEHAMTVLTEGDR
CEEECCCCEEEECCCCHHHHHHHHHCCCCCCCCCEEEEECCEEEEECCCCEEEEEECCCE
LEIVQFVGGG
EEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA