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The map label for this gene is ispE

Identifier: 114798429

GI number: 114798429

Start: 685501

End: 686397

Strand: Direct

Name: ispE

Synonym: HNE_0676

Alternate gene names: 114798429

Gene position: 685501-686397 (Clockwise)

Preceding gene: 114799492

Following gene: 114797664

Centisome position: 18.5

GC content: 67.56

Gene sequence:

>897_bases
ATGAGCACAGCAGACAAAAGCCTTTCGGGCTGGGCGCCTGCCAAGGTGAACCTCTATCTGCATGTCGGCCCGCCAAAACC
CAATGGCCGCCATGACCTTGAGTCTCTTGTAATGTTCTCGGGTTCTGGTGCGGCAGACCATCTGACGGCAGAGCCTGCCG
AAGGGCTCAGCCTTTCGGTTGCCGGCCCCTTCGCCGCTGCGGCCGGGGCGGGCGATGACAATCTGGTCCTTCAGGCGGCC
CGCGCGCTACAGGCCGCCTCCGGCACGCAACAAGGCGCCCGTCTTTCGCTCAAGAAATGGCTGCCCGTCGCCGCCGGCAT
CGGCGGTGGCTCCTCCGACGCCGCCACAGCCCTCCGTCTGCTGACAAAACTCTGGGCGCTTGACCCAAACCATGCCATCG
CCCTTGCGCCGGGCCTTGGAGGGGATGTGCCGGTAGCGTTGGCGGGGCAGCCATCACTCATGCGCGGGGAAGGGGAGCGC
GTCACGCCGCTGCCCGCGCTGCCGCCCGTTTATGCCGTGCTGGTCAATCCCGGCGTCCCATGCCCCACAGGCCCGGTCTT
CCGCGACCATGACGCGGCAGGGGGCGGCGCGGGCTTTGCCGAGATTGATCCGCCACCGGAGTTTTCAGGGCCCAAAGCAT
TCGCCGCCTGGCTCGGCCAGCAACGCAACGATCTGGAAAGCCCTGCCATCGCGCGCGTGCCGGAAATCGGCGCCGTGCTC
GAATTCCTGCGCGCCCAGCCCGGCGTATTGCTGGCGCGCATGAGCGGGTCAGGTGCCACCTGTTTTGCCCTCTGTGAAGC
GCTCGCCTTTGCGGAGCGCGCAACCGCCGTCATCGCCCATGATGCGCACAAGGCGCATTGGTGGACCGCTGCCTCCCGGC
TCGGGGCGGCGCCATGA

Upstream 100 bases:

>100_bases
GCCAAGCTCCATTCAAAGCTGGGCCCCGATGCGCCCGAAGCAGCGGCGGAGTCAAAATCTCCGACCGTCACGCAAGACTC
TCCCTGAGGGGCGCTTCCGG

Downstream 100 bases:

>100_bases
TCGCGCTTTCCCTTGTCTGGGCCGCGGCCCCGCTCATCATCAGCTTTCTCGTCTGCGCGCTTATGATCCGCCTTGGCCCG
AAAGACGCGCCTGATGGCGG

Product: 4-diphosphocytidyl-2C-methyl-D-erythritol kinase

Products: NA

Alternate protein names: CMK; 4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSTADKSLSGWAPAKVNLYLHVGPPKPNGRHDLESLVMFSGSGAADHLTAEPAEGLSLSVAGPFAAAAGAGDDNLVLQAA
RALQAASGTQQGARLSLKKWLPVAAGIGGGSSDAATALRLLTKLWALDPNHAIALAPGLGGDVPVALAGQPSLMRGEGER
VTPLPALPPVYAVLVNPGVPCPTGPVFRDHDAAGGGAGFAEIDPPPEFSGPKAFAAWLGQQRNDLESPAIARVPEIGAVL
EFLRAQPGVLLARMSGSGATCFALCEALAFAERATAVIAHDAHKAHWWTAASRLGAAP

Sequences:

>Translated_298_residues
MSTADKSLSGWAPAKVNLYLHVGPPKPNGRHDLESLVMFSGSGAADHLTAEPAEGLSLSVAGPFAAAAGAGDDNLVLQAA
RALQAASGTQQGARLSLKKWLPVAAGIGGGSSDAATALRLLTKLWALDPNHAIALAPGLGGDVPVALAGQPSLMRGEGER
VTPLPALPPVYAVLVNPGVPCPTGPVFRDHDAAGGGAGFAEIDPPPEFSGPKAFAAWLGQQRNDLESPAIARVPEIGAVL
EFLRAQPGVLLARMSGSGATCFALCEALAFAERATAVIAHDAHKAHWWTAASRLGAAP
>Mature_297_residues
STADKSLSGWAPAKVNLYLHVGPPKPNGRHDLESLVMFSGSGAADHLTAEPAEGLSLSVAGPFAAAAGAGDDNLVLQAAR
ALQAASGTQQGARLSLKKWLPVAAGIGGGSSDAATALRLLTKLWALDPNHAIALAPGLGGDVPVALAGQPSLMRGEGERV
TPLPALPPVYAVLVNPGVPCPTGPVFRDHDAAGGGAGFAEIDPPPEFSGPKAFAAWLGQQRNDLESPAIARVPEIGAVLE
FLRAQPGVLLARMSGSGATCFALCEALAFAERATAVIAHDAHKAHWWTAASRLGAAP

Specific function: Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol

COG id: COG1947

COG function: function code I; 4-diphosphocytidyl-2C-methyl-D-erythritol 2-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GHMP kinase family. IspE subfamily

Homologues:

Organism=Escherichia coli, GI1787459, Length=261, Percent_Identity=32.183908045977, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPE_HYPNA (Q0C4D8)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759405.1
- ProteinModelPortal:   Q0C4D8
- SMR:   Q0C4D8
- STRING:   Q0C4D8
- GeneID:   4288168
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0676
- NMPDR:   fig|228405.5.peg.663
- TIGR:   HNE_0676
- eggNOG:   COG1947
- HOGENOM:   HBG734593
- OMA:   ELAMIMQ
- PhylomeDB:   Q0C4D8
- BioCyc:   HNEP81032:HNE_0676-MONOMER
- HAMAP:   MF_00061
- InterPro:   IPR006204
- InterPro:   IPR013750
- InterPro:   IPR004424
- InterPro:   IPR020568
- InterPro:   IPR014721
- Gene3D:   G3DSA:3.30.230.10
- PANTHER:   PTHR20861:SF2
- PIRSF:   PIRSF010376

Pfam domain/function: PF08544 GHMP_kinases_C; PF00288 GHMP_kinases_N; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.1.148

Molecular weight: Translated: 30216; Mature: 30084

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: ACT_SITE 15-15 ACT_SITE 142-142

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTADKSLSGWAPAKVNLYLHVGPPKPNGRHDLESLVMFSGSGAADHLTAEPAEGLSLSV
CCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHEECCCCCCCCCCCCCCCCCEEEE
AGPFAAAAGAGDDNLVLQAARALQAASGTQQGARLSLKKWLPVAAGIGGGSSDAATALRL
CCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHCCHHCCCCCCCHHHHHHHHH
LTKLWALDPNHAIALAPGLGGDVPVALAGQPSLMRGEGERVTPLPALPPVYAVLVNPGVP
HHHHHHCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
CPTGPVFRDHDAAGGGAGFAEIDPPPEFSGPKAFAAWLGQQRNDLESPAIARVPEIGAVL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHCCCCCCHHHCCCHHHHHH
EFLRAQPGVLLARMSGSGATCFALCEALAFAERATAVIAHDAHKAHWWTAASRLGAAP
HHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STADKSLSGWAPAKVNLYLHVGPPKPNGRHDLESLVMFSGSGAADHLTAEPAEGLSLSV
CCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHEECCCCCCCCCCCCCCCCCEEEE
AGPFAAAAGAGDDNLVLQAARALQAASGTQQGARLSLKKWLPVAAGIGGGSSDAATALRL
CCCHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHCCHHCCCCCCCHHHHHHHHH
LTKLWALDPNHAIALAPGLGGDVPVALAGQPSLMRGEGERVTPLPALPPVYAVLVNPGVP
HHHHHHCCCCCEEEECCCCCCCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCC
CPTGPVFRDHDAAGGGAGFAEIDPPPEFSGPKAFAAWLGQQRNDLESPAIARVPEIGAVL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCHHCCCCCCHHHCCCHHHHHH
EFLRAQPGVLLARMSGSGATCFALCEALAFAERATAVIAHDAHKAHWWTAASRLGAAP
HHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA