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The map label for this gene is ghrA [H]

Identifier: 114798418

GI number: 114798418

Start: 2951264

End: 2952259

Strand: Reverse

Name: ghrA [H]

Synonym: HNE_2789

Alternate gene names: 114798418

Gene position: 2952259-2951264 (Counterclockwise)

Preceding gene: 114799696

Following gene: 114799604

Centisome position: 79.68

GC content: 64.06

Gene sequence:

>996_bases
ATGAAAACACTTCTTCTTCATGCGCGGACATTCGCGCGCATCGCGCCCCGGCTCAAGGGGCTGAACGCCGCTCTGAATAT
CGTGACCGTTGATGATGCGGCCCGGTTCCATGATGCCTGGACCTCGGAGGCGCTCAAGGAGGCGCCGGCGGTGGACCTCG
CCTTTGGCAATATTGACGCCTTCTATTCCCCGTCTGTGCGGGAATTCATGACGGCAATCCTGAAAAGCCCCGATTTTGAC
TGGTTCCAGTCTGCGGCTGCCGGCATCGAGAACCCGGCCCTGGTGGCCATCGGGCAGAAATCGAAGCGCTATACGACCAA
CCATACCCAGGCCGAATCGATGGCCGAATGGGCGATCTGGCAGGCGATGGACTTCTTCCGGGACGGGCCGGGCCACCGGG
CCAATCAGGCGCGGGGGGAATGGAAGCGGATTGCTGTGCGGGAAATGCAGGGCTCGCGCTGGCTGATCGTTGGCTATGGC
TCGATCGGGCAGGCGGTTGGCAAGCGTGTGAGCGCGCTGGGCGGGGTGGTGACCGGCATGCGCCGCACGCCGGGGCCGGC
AGGCGGCGCAGCCCGTGTTGTGGCGCCCGATACGCTGATGGCGGAATTGCCCGGCGCCGATGTCGTCTTGCTGTGCCCGC
CGCACACGCCGGAGACCGAAGGCATGGCGGACGCGGCGTTCTTTGCGGCGATGAACCCGGACGCGCTGTTTCTCAACCTC
GGGCGCGGCGCGCTGGTGGATGAGGACGCTTTGATGGCGGCGCTGGACGCTGGCAGGCCAGCCTTTGCCGCGCTGGATGT
GACCAAAACGGAGCCTTTGCCCGAAGACAGCGCGCTCTGGAAACATCACAAAATCGTGATCACGCCGCACGACAGCTCCG
ATACGCCGGGCACAGTTGCGCGCGGCGACGAGACTTTCCTTGCCAACCTCCAGCTCTATCTGGACAATGAACCCATGAAA
CATCTCGTTGATCGCTCGGCCTTCCTGGCCGGTTGA

Upstream 100 bases:

>100_bases
CGTCGCGAAAATAATCGGGGCCCCGCACCGGCAGGTTTCCCTGCGGCAGGGCGGGCATCATGCTTGTGCTGGCGCCAAGG
GCCAAGCTAAGCAGGAGGGC

Downstream 100 bases:

>100_bases
TCCGGATGCAGAGGAGTGAGTGATATGACACAATTCAAGCTGGTTTCCGCAGCCGCCATCGCGGCAGCCCTGACCCTCAC
AGCCTGCGCGACTGAGAATT

Product: D-isomer specific 2-hydroxyacid dehydrogenase family protein

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKTLLLHARTFARIAPRLKGLNAALNIVTVDDAARFHDAWTSEALKEAPAVDLAFGNIDAFYSPSVREFMTAILKSPDFD
WFQSAAAGIENPALVAIGQKSKRYTTNHTQAESMAEWAIWQAMDFFRDGPGHRANQARGEWKRIAVREMQGSRWLIVGYG
SIGQAVGKRVSALGGVVTGMRRTPGPAGGAARVVAPDTLMAELPGADVVLLCPPHTPETEGMADAAFFAAMNPDALFLNL
GRGALVDEDALMAALDAGRPAFAALDVTKTEPLPEDSALWKHHKIVITPHDSSDTPGTVARGDETFLANLQLYLDNEPMK
HLVDRSAFLAG

Sequences:

>Translated_331_residues
MKTLLLHARTFARIAPRLKGLNAALNIVTVDDAARFHDAWTSEALKEAPAVDLAFGNIDAFYSPSVREFMTAILKSPDFD
WFQSAAAGIENPALVAIGQKSKRYTTNHTQAESMAEWAIWQAMDFFRDGPGHRANQARGEWKRIAVREMQGSRWLIVGYG
SIGQAVGKRVSALGGVVTGMRRTPGPAGGAARVVAPDTLMAELPGADVVLLCPPHTPETEGMADAAFFAAMNPDALFLNL
GRGALVDEDALMAALDAGRPAFAALDVTKTEPLPEDSALWKHHKIVITPHDSSDTPGTVARGDETFLANLQLYLDNEPMK
HLVDRSAFLAG
>Mature_331_residues
MKTLLLHARTFARIAPRLKGLNAALNIVTVDDAARFHDAWTSEALKEAPAVDLAFGNIDAFYSPSVREFMTAILKSPDFD
WFQSAAAGIENPALVAIGQKSKRYTTNHTQAESMAEWAIWQAMDFFRDGPGHRANQARGEWKRIAVREMQGSRWLIVGYG
SIGQAVGKRVSALGGVVTGMRRTPGPAGGAARVVAPDTLMAELPGADVVLLCPPHTPETEGMADAAFFAAMNPDALFLNL
GRGALVDEDALMAALDAGRPAFAALDVTKTEPLPEDSALWKHHKIVITPHDSSDTPGTVARGDETFLANLQLYLDNEPMK
HLVDRSAFLAG

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=256, Percent_Identity=31.640625, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI23308577, Length=247, Percent_Identity=28.7449392712551, Blast_Score=86, Evalue=6e-17,
Organism=Escherichia coli, GI87081824, Length=184, Percent_Identity=32.6086956521739, Blast_Score=98, Evalue=9e-22,
Organism=Escherichia coli, GI1789279, Length=194, Percent_Identity=33.5051546391753, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI87082289, Length=148, Percent_Identity=40.5405405405405, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17532191, Length=216, Percent_Identity=27.7777777777778, Blast_Score=68, Evalue=7e-12,
Organism=Saccharomyces cerevisiae, GI6322116, Length=196, Percent_Identity=33.6734693877551, Blast_Score=81, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320925, Length=196, Percent_Identity=33.6734693877551, Blast_Score=81, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6325144, Length=166, Percent_Identity=27.1084337349398, Blast_Score=68, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28571528, Length=149, Percent_Identity=36.9127516778523, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI45552429, Length=169, Percent_Identity=36.6863905325444, Blast_Score=83, Evalue=3e-16,
Organism=Drosophila melanogaster, GI45551003, Length=169, Percent_Identity=36.6863905325444, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI28574284, Length=169, Percent_Identity=36.6863905325444, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24585514, Length=169, Percent_Identity=36.6863905325444, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28574282, Length=169, Percent_Identity=36.6863905325444, Blast_Score=82, Evalue=5e-16,
Organism=Drosophila melanogaster, GI28574286, Length=171, Percent_Identity=36.2573099415205, Blast_Score=79, Evalue=4e-15,
Organism=Drosophila melanogaster, GI24585516, Length=195, Percent_Identity=28.7179487179487, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19921140, Length=168, Percent_Identity=30.3571428571429, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35685; Mature: 35685

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTLLLHARTFARIAPRLKGLNAALNIVTVDDAARFHDAWTSEALKEAPAVDLAFGNIDA
CCEEEEHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCC
FYSPSVREFMTAILKSPDFDWFQSAAAGIENPALVAIGQKSKRYTTNHTQAESMAEWAIW
CCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
QAMDFFRDGPGHRANQARGEWKRIAVREMQGSRWLIVGYGSIGQAVGKRVSALGGVVTGM
HHHHHHHCCCCCCCCHHCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCC
RRTPGPAGGAARVVAPDTLMAELPGADVVLLCPPHTPETEGMADAAFFAAMNPDALFLNL
CCCCCCCCCCEEEECCHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHEEECCCCEEEEEC
GRGALVDEDALMAALDAGRPAFAALDVTKTEPLPEDSALWKHHKIVITPHDSSDTPGTVA
CCCCEECHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHCCEEEEEEECCCCCCCCCEE
RGDETFLANLQLYLDNEPMKHLVDRSAFLAG
CCCCEEEEEEEEEECCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MKTLLLHARTFARIAPRLKGLNAALNIVTVDDAARFHDAWTSEALKEAPAVDLAFGNIDA
CCEEEEHHHHHHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHCCCEEEEECCCCC
FYSPSVREFMTAILKSPDFDWFQSAAAGIENPALVAIGQKSKRYTTNHTQAESMAEWAIW
CCCCCHHHHHHHHHCCCCHHHHHHHHCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
QAMDFFRDGPGHRANQARGEWKRIAVREMQGSRWLIVGYGSIGQAVGKRVSALGGVVTGM
HHHHHHHCCCCCCCCHHCCHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHHHCC
RRTPGPAGGAARVVAPDTLMAELPGADVVLLCPPHTPETEGMADAAFFAAMNPDALFLNL
CCCCCCCCCCEEEECCHHHHHHCCCCCEEEECCCCCCCCCCCHHHHHEEECCCCEEEEEC
GRGALVDEDALMAALDAGRPAFAALDVTKTEPLPEDSALWKHHKIVITPHDSSDTPGTVA
CCCCEECHHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHCCEEEEEEECCCCCCCCCEE
RGDETFLANLQLYLDNEPMKHLVDRSAFLAG
CCCCEEEEEEEEEECCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA