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The map label for this gene is metK

Identifier: 114798411

GI number: 114798411

Start: 798390

End: 799571

Strand: Reverse

Name: metK

Synonym: HNE_0786

Alternate gene names: 114798411

Gene position: 799571-798390 (Counterclockwise)

Preceding gene: 114797552

Following gene: 114800368

Centisome position: 21.58

GC content: 62.77

Gene sequence:

>1182_bases
ATGCCCCTGAAGTCCCATGAGTTCACCAGCGAGAGCGTCGCCGAAGGCCATCCCGACAAGGTTGCCGACCAGATTTCCGA
CGCGATTGTCGATCTGTTCCTGAGCAAGGACCCGACCTCGAAAGTCGCCGTCGAAACGCTGTGCACGACCAATCGCGTGG
TCCTGGCCGGCGAAGTGCGCACCAATAACGGCTCCGTCGTCACGCCTGACGAAATGAACGCTGCGGCCCGCGCCGTTGTA
AAAAAAATCGGCTATGAGCAGGACGGCTTCCATTGGGAAAAGATGGCGGTTGAAAACCACGTCCACGGACAATCTGCCGA
GATTGCCCAGGGCGTTGAAGAAGGTCAGGGCCTCTTCAAGGAAGAAGGCGCCGGCGACCAGGGCATCATGTTCGGCTATG
CCACCGATGAAACGCCCGAGCTGATGCCCGCCACGCTCGTCTACTCTCACCAGATCCTCCAGAAACTGGCGGAGCTGCGC
CATTCAGGCAAGAACCCGCAGCTTGAGCCCGACGCCAAAAGCCAGGTGACGCTCCAGTATGAAGGCTCCCGTCCGGTTGG
CGTCAACGCGGTCGTTGTTTCTCACCAGCACAAGGCCGGCGTCAGCCAGGAAGAGCTGCGCGAGGTCATCCGCCCGGTGG
TAAAAGCCGTCCTCCCCGAGGGCTGGTTCCCGCCCGAAGAGAAATTCTACGTCAACCCCACCGGCAGCTTCGTCATCGGC
GGCCCCGATGGCGACTGTGGTCTCACCGGCCGCAAGATCATCGTCGACACCTATGGCGGCGCAGCCCCCCATGGCGGCGG
CGCGTTCTCGGGTAAAGACCCGTCCAAGGTTGACCGCTCGGCCGCCTATGCCACGCGCTACCTCGCCAAGAACATCGTTG
CCGCGGGCCTCGCCACGCGCTGCACCATCCAGGTGTCCTACGCGATCGGCGTTGCCCAGCCGCTGTCGATCTATGTCGAC
ACCCACGGCACCGGCCAGGCCGACGAAGCCCGCATCGCCAAAGCCGTGCGGGAGCTTTTCGACCTCTCGCCCAAAGGCAT
CCGCACGCATCTGAAGCTGTCCAACCCGATCTACGGGCCAACAGCCGCCTACGGACATTTCGGCCGCATCCCCGGCGAGG
GTGGCACCTTCACCTGGGAAAAAACCGACCTGGCCGGCGAACTGAAACGCCTCGTCAACTAA

Upstream 100 bases:

>100_bases
CTTCTGCGGGCGGTAAAAGCCCATGCCGAGCACCGCATTTTCCTGAATTCCGGCCGCACAGTCGTGTTCGCCCGGTAAGC
CATTCCAAGAGGAGCCAAGA

Downstream 100 bases:

>100_bases
GCGATTTGGGGGGACAGGCCCTGCCCTGTCCCCCGCACGCCTCTCCCTGCCTAAGGTCAGGAAAGGCATCCGGGAAGAGG
CAAGCTCGTGCATCGCCCTC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MPLKSHEFTSESVAEGHPDKVADQISDAIVDLFLSKDPTSKVAVETLCTTNRVVLAGEVRTNNGSVVTPDEMNAAARAVV
KKIGYEQDGFHWEKMAVENHVHGQSAEIAQGVEEGQGLFKEEGAGDQGIMFGYATDETPELMPATLVYSHQILQKLAELR
HSGKNPQLEPDAKSQVTLQYEGSRPVGVNAVVVSHQHKAGVSQEELREVIRPVVKAVLPEGWFPPEEKFYVNPTGSFVIG
GPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYATRYLAKNIVAAGLATRCTIQVSYAIGVAQPLSIYVD
THGTGQADEARIAKAVRELFDLSPKGIRTHLKLSNPIYGPTAAYGHFGRIPGEGGTFTWEKTDLAGELKRLVN

Sequences:

>Translated_393_residues
MPLKSHEFTSESVAEGHPDKVADQISDAIVDLFLSKDPTSKVAVETLCTTNRVVLAGEVRTNNGSVVTPDEMNAAARAVV
KKIGYEQDGFHWEKMAVENHVHGQSAEIAQGVEEGQGLFKEEGAGDQGIMFGYATDETPELMPATLVYSHQILQKLAELR
HSGKNPQLEPDAKSQVTLQYEGSRPVGVNAVVVSHQHKAGVSQEELREVIRPVVKAVLPEGWFPPEEKFYVNPTGSFVIG
GPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYATRYLAKNIVAAGLATRCTIQVSYAIGVAQPLSIYVD
THGTGQADEARIAKAVRELFDLSPKGIRTHLKLSNPIYGPTAAYGHFGRIPGEGGTFTWEKTDLAGELKRLVN
>Mature_392_residues
PLKSHEFTSESVAEGHPDKVADQISDAIVDLFLSKDPTSKVAVETLCTTNRVVLAGEVRTNNGSVVTPDEMNAAARAVVK
KIGYEQDGFHWEKMAVENHVHGQSAEIAQGVEEGQGLFKEEGAGDQGIMFGYATDETPELMPATLVYSHQILQKLAELRH
SGKNPQLEPDAKSQVTLQYEGSRPVGVNAVVVSHQHKAGVSQEELREVIRPVVKAVLPEGWFPPEEKFYVNPTGSFVIGG
PDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYATRYLAKNIVAAGLATRCTIQVSYAIGVAQPLSIYVDT
HGTGQADEARIAKAVRELFDLSPKGIRTHLKLSNPIYGPTAAYGHFGRIPGEGGTFTWEKTDLAGELKRLVN

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family

Homologues:

Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=50.5263157894737, Blast_Score=338, Evalue=6e-93,
Organism=Homo sapiens, GI4557737, Length=380, Percent_Identity=49.2105263157895, Blast_Score=335, Evalue=6e-92,
Organism=Escherichia coli, GI1789311, Length=390, Percent_Identity=55.6410256410256, Blast_Score=412, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17538498, Length=388, Percent_Identity=51.2886597938144, Blast_Score=344, Evalue=4e-95,
Organism=Caenorhabditis elegans, GI25145633, Length=381, Percent_Identity=51.4435695538058, Blast_Score=344, Evalue=6e-95,
Organism=Caenorhabditis elegans, GI17538494, Length=381, Percent_Identity=51.7060367454068, Blast_Score=343, Evalue=1e-94,
Organism=Caenorhabditis elegans, GI17551082, Length=381, Percent_Identity=49.0813648293963, Blast_Score=325, Evalue=3e-89,
Organism=Caenorhabditis elegans, GI32565905, Length=379, Percent_Identity=48.0211081794195, Blast_Score=303, Evalue=7e-83,
Organism=Caenorhabditis elegans, GI25145635, Length=336, Percent_Identity=50, Blast_Score=286, Evalue=9e-78,
Organism=Caenorhabditis elegans, GI32565911, Length=336, Percent_Identity=50.297619047619, Blast_Score=285, Evalue=2e-77,
Organism=Caenorhabditis elegans, GI32565903, Length=300, Percent_Identity=52.3333333333333, Blast_Score=273, Evalue=1e-73,
Organism=Caenorhabditis elegans, GI32565909, Length=79, Percent_Identity=50.6329113924051, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320710, Length=385, Percent_Identity=47.5324675324675, Blast_Score=332, Evalue=4e-92,
Organism=Saccharomyces cerevisiae, GI6323209, Length=381, Percent_Identity=47.7690288713911, Blast_Score=327, Evalue=2e-90,
Organism=Drosophila melanogaster, GI24580527, Length=384, Percent_Identity=49.4791666666667, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24580525, Length=384, Percent_Identity=49.4791666666667, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI45552159, Length=381, Percent_Identity=49.8687664041995, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24580529, Length=381, Percent_Identity=49.8687664041995, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24580537, Length=381, Percent_Identity=49.8687664041995, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24580533, Length=381, Percent_Identity=49.8687664041995, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24580535, Length=381, Percent_Identity=49.8687664041995, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24580531, Length=381, Percent_Identity=49.8687664041995, Blast_Score=314, Evalue=6e-86,
Organism=Drosophila melanogaster, GI24580541, Length=373, Percent_Identity=48.2573726541555, Blast_Score=296, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24580539, Length=125, Percent_Identity=44, Blast_Score=90, Evalue=3e-18,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): METK_HYPNA (Q0C429)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_759514.1
- ProteinModelPortal:   Q0C429
- SMR:   Q0C429
- STRING:   Q0C429
- GeneID:   4289752
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0786
- NMPDR:   fig|228405.5.peg.768
- TIGR:   HNE_0786
- eggNOG:   COG0192
- HOGENOM:   HBG443662
- OMA:   RACIKDI
- ProtClustDB:   PRK05250
- BioCyc:   HNEP81032:HNE_0786-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00086
- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636
- PANTHER:   PTHR11964
- PIRSF:   PIRSF000497
- TIGRFAMs:   TIGR01034

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N; SSF55973 S-AdoMet_synt

EC number: =2.5.1.6

Molecular weight: Translated: 42156; Mature: 42025

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00376 ADOMET_SYNTHASE_1; PS00377 ADOMET_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLKSHEFTSESVAEGHPDKVADQISDAIVDLFLSKDPTSKVAVETLCTTNRVVLAGEVR
CCCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEE
TNNGSVVTPDEMNAAARAVVKKIGYEQDGFHWEKMAVENHVHGQSAEIAQGVEEGQGLFK
ECCCCEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCHHH
EEGAGDQGIMFGYATDETPELMPATLVYSHQILQKLAELRHSGKNPQLEPDAKSQVTLQY
HCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
EGSRPVGVNAVVVSHQHKAGVSQEELREVIRPVVKAVLPEGWFPPEEKFYVNPTGSFVIG
CCCCCCCCEEEEEECCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEE
GPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYATRYLAKNIVAAGLATR
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
CTIQVSYAIGVAQPLSIYVDTHGTGQADEARIAKAVRELFDLSPKGIRTHLKLSNPIYGP
EEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
TAAYGHFGRIPGEGGTFTWEKTDLAGELKRLVN
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCC
>Mature Secondary Structure 
PLKSHEFTSESVAEGHPDKVADQISDAIVDLFLSKDPTSKVAVETLCTTNRVVLAGEVR
CCCCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCEEEEEEEEE
TNNGSVVTPDEMNAAARAVVKKIGYEQDGFHWEKMAVENHVHGQSAEIAQGVEEGQGLFK
ECCCCEECCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCHHH
EEGAGDQGIMFGYATDETPELMPATLVYSHQILQKLAELRHSGKNPQLEPDAKSQVTLQY
HCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEE
EGSRPVGVNAVVVSHQHKAGVSQEELREVIRPVVKAVLPEGWFPPEEKFYVNPTGSFVIG
CCCCCCCCEEEEEECCHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEE
GPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKDPSKVDRSAAYATRYLAKNIVAAGLATR
CCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEE
CTIQVSYAIGVAQPLSIYVDTHGTGQADEARIAKAVRELFDLSPKGIRTHLKLSNPIYGP
EEEEEEEEECCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCC
TAAYGHFGRIPGEGGTFTWEKTDLAGELKRLVN
CCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA