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The map label for this gene is alkK [H]

Identifier: 114798331

GI number: 114798331

Start: 849439

End: 851064

Strand: Direct

Name: alkK [H]

Synonym: HNE_0828

Alternate gene names: 114798331

Gene position: 849439-851064 (Clockwise)

Preceding gene: 114799860

Following gene: 114799627

Centisome position: 22.93

GC content: 62.42

Gene sequence:

>1626_bases
ATGAAGGGCATTATGCAGGACTGGCCGCTGACCGTGGACAAGATCCTCGAGCATGCGCGCCTGCAACACGGGCAGCGGGA
GATCATCACCCGCCGCGTGGAAGGCAACATCACGCGCACGACCTATTCGCAGCTGTATGACATGTCCAAGCAGGTTTCGA
CCGCGCTGAAGGATGAAGGCATTGCGCTCGGCGACCGGGTGGCGACGCTCGGCTGGAACTCCGAGCGGCATATGGCGAGC
TGGTATGGCGCGATGGGCATCGGCGCGGTGCTGCACACGATCAATCCGCGCCTGCACCCCGAGCAGATCGCCTGGATTGC
CAACCATGCCGAAGACAAGGTTCTGATCTTCGACAAGACGTTCCTGCCGATCGTGGAAGCGATCAAGGACAAGCTGGAGA
CGGTAAAGACGTTCGTGATCTATGCCGGCGCCGACACGATGCCGGAAAATACGCTCGGCGCGATTGCGTTCGACATCTGG
ATCGACGGGCGCAGCAAGTCCGTGCGTTGGGGCGACTTTCCTGAAGATACCGCCTGCGGGCTTTGTTACACCTCCGGCAC
GACGGGCAACCCCAAGGGCGTGCTCTATTCCCACCGCTCCAACGTGCTGCACACGCTTGTGACCATGGGCAAGGACGCGA
TGGGCATGGGGGCGGCCGACTCGGTGATGCCGGTGGTGCCGATGTTCCATGCCAATGCCTGGGGGCTGGCGATGTCCTGC
CCGGCGACGGGGGCCAATATGGTGATGCCCGGCGCGCAGATGGACGGGGCGTCGATCTATGAGCTGCTGGATTCGGAGAA
GGTGTCGCTGACCGCCGCCGTGCCGACTGTGTGGCTGATGCTGCTGACGCACCTTCAGCAGAATGATCTGAAGCTGCCTT
ATCTGAAGAAGGTGCTGATCGGGGGCTCTGCGATTCCGGAGAAGATCCTGCGCGCGTTTGAAGAGGAATATGAAGTTGAG
GTGATCCATGCCTGGGGCATGACCGAGACCTCGCCGCTGGGCACGCTGGGCGCGCTGCCGCCGCATCTGGAGAAGGCGCC
GGTCGACGCGCGGATGAAACAGAAGCTGAAGCAGGGCCGCCCGCCCTTTGGCGTTGAGCTGAAGCTGGTGGATGATGACG
GCGAGGATGTGGCGCGCGACGGGCAGGCTTCCGGCCGGCTTCTGGTGCGCGGCGCGGCCGTTGCGGCGGGCTATTTCAAG
GGCGCGGGCGGACATGTTCTGGATGCGGACGGCTGGTTTGATACCGGGGACGTGGCGACCATCGACGAGATCGGCACGAT
GACGATCACTGACCGGGCCAAGGATGTGATCAAGTCGGGCGGGGAGTGGATTTCCTCCATCGACATCGAGAACATTGCCG
TGGGCCATCCCAAGGTCGCCAACGCTGCGGCGATCGGCATCTACCATCCCAAATGGGATGAGCGCCCGTTGCTGATCGTT
CAGGCGGCGGCCGGGGAAACTCCGACCAAGGAGGAAATCCTCAAAAGCCTCGAAGGCAAGATCGCCAAATGGTGGACGCC
GGACGATGTGCAGTTTGTGGACAACATTCCGCTGGGCGCGACGGGGAAGATCAACAAGCTGGCGCTGCGCGAGACGTTCA
AGGACTATAAGCTTCCGACTGCCTGA

Upstream 100 bases:

>100_bases
CTTGCGTCAGCGTATTTGCCCGAATAGACAGGGCGTTGATAAATCACAAGCAGAACCCAACGCGGCAGGACCAACAAGAA
CCGTTCCGGAGGAGAATTAC

Downstream 100 bases:

>100_bases
GGCCCCTGATGCAACGAGATGTCCTGTTCCTGCTGAAGCCCGGGTTTCACGATCCGGACCTGCCGGGGCAGGACTTCTAT
TGCTGGCATTGCGCGCTGAT

Product: putative acyl-CoA synthetase

Products: D-carnitinyl-CoA; crotonobetainyl-CoA; L-carnitinyl-CoA [C]

Alternate protein names: Medium-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 541; Mature: 541

Protein sequence:

>541_residues
MKGIMQDWPLTVDKILEHARLQHGQREIITRRVEGNITRTTYSQLYDMSKQVSTALKDEGIALGDRVATLGWNSERHMAS
WYGAMGIGAVLHTINPRLHPEQIAWIANHAEDKVLIFDKTFLPIVEAIKDKLETVKTFVIYAGADTMPENTLGAIAFDIW
IDGRSKSVRWGDFPEDTACGLCYTSGTTGNPKGVLYSHRSNVLHTLVTMGKDAMGMGAADSVMPVVPMFHANAWGLAMSC
PATGANMVMPGAQMDGASIYELLDSEKVSLTAAVPTVWLMLLTHLQQNDLKLPYLKKVLIGGSAIPEKILRAFEEEYEVE
VIHAWGMTETSPLGTLGALPPHLEKAPVDARMKQKLKQGRPPFGVELKLVDDDGEDVARDGQASGRLLVRGAAVAAGYFK
GAGGHVLDADGWFDTGDVATIDEIGTMTITDRAKDVIKSGGEWISSIDIENIAVGHPKVANAAAIGIYHPKWDERPLLIV
QAAAGETPTKEEILKSLEGKIAKWWTPDDVQFVDNIPLGATGKINKLALRETFKDYKLPTA

Sequences:

>Translated_541_residues
MKGIMQDWPLTVDKILEHARLQHGQREIITRRVEGNITRTTYSQLYDMSKQVSTALKDEGIALGDRVATLGWNSERHMAS
WYGAMGIGAVLHTINPRLHPEQIAWIANHAEDKVLIFDKTFLPIVEAIKDKLETVKTFVIYAGADTMPENTLGAIAFDIW
IDGRSKSVRWGDFPEDTACGLCYTSGTTGNPKGVLYSHRSNVLHTLVTMGKDAMGMGAADSVMPVVPMFHANAWGLAMSC
PATGANMVMPGAQMDGASIYELLDSEKVSLTAAVPTVWLMLLTHLQQNDLKLPYLKKVLIGGSAIPEKILRAFEEEYEVE
VIHAWGMTETSPLGTLGALPPHLEKAPVDARMKQKLKQGRPPFGVELKLVDDDGEDVARDGQASGRLLVRGAAVAAGYFK
GAGGHVLDADGWFDTGDVATIDEIGTMTITDRAKDVIKSGGEWISSIDIENIAVGHPKVANAAAIGIYHPKWDERPLLIV
QAAAGETPTKEEILKSLEGKIAKWWTPDDVQFVDNIPLGATGKINKLALRETFKDYKLPTA
>Mature_541_residues
MKGIMQDWPLTVDKILEHARLQHGQREIITRRVEGNITRTTYSQLYDMSKQVSTALKDEGIALGDRVATLGWNSERHMAS
WYGAMGIGAVLHTINPRLHPEQIAWIANHAEDKVLIFDKTFLPIVEAIKDKLETVKTFVIYAGADTMPENTLGAIAFDIW
IDGRSKSVRWGDFPEDTACGLCYTSGTTGNPKGVLYSHRSNVLHTLVTMGKDAMGMGAADSVMPVVPMFHANAWGLAMSC
PATGANMVMPGAQMDGASIYELLDSEKVSLTAAVPTVWLMLLTHLQQNDLKLPYLKKVLIGGSAIPEKILRAFEEEYEVE
VIHAWGMTETSPLGTLGALPPHLEKAPVDARMKQKLKQGRPPFGVELKLVDDDGEDVARDGQASGRLLVRGAAVAAGYFK
GAGGHVLDADGWFDTGDVATIDEIGTMTITDRAKDVIKSGGEWISSIDIENIAVGHPKVANAAAIGIYHPKWDERPLLIV
QAAAGETPTKEEILKSLEGKIAKWWTPDDVQFVDNIPLGATGKINKLALRETFKDYKLPTA

Specific function: Could Catalyze The Transfer Of CoA To Crotonobetaine Or Carnitine. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=554, Percent_Identity=24.5487364620939, Blast_Score=152, Evalue=6e-37,
Organism=Homo sapiens, GI187761345, Length=549, Percent_Identity=25.1366120218579, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI187761343, Length=549, Percent_Identity=25.1366120218579, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI28416953, Length=568, Percent_Identity=25.3521126760563, Blast_Score=96, Evalue=1e-19,
Organism=Homo sapiens, GI157311624, Length=519, Percent_Identity=22.1579961464355, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI157311622, Length=519, Percent_Identity=22.1579961464355, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI58082049, Length=523, Percent_Identity=22.3709369024857, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI42544132, Length=524, Percent_Identity=21.7557251908397, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI122937307, Length=518, Percent_Identity=22.5868725868726, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI115511026, Length=534, Percent_Identity=20.9737827715356, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI13375727, Length=571, Percent_Identity=21.8914185639229, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI221142682, Length=509, Percent_Identity=24.5579567779961, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI145693145, Length=500, Percent_Identity=26.6, Blast_Score=148, Evalue=9e-37,
Organism=Escherichia coli, GI1788107, Length=521, Percent_Identity=26.4875239923225, Blast_Score=127, Evalue=3e-30,
Organism=Escherichia coli, GI1786810, Length=553, Percent_Identity=24.9547920433996, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789201, Length=372, Percent_Identity=23.9247311827957, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1788595, Length=533, Percent_Identity=21.0131332082552, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1790505, Length=380, Percent_Identity=26.3157894736842, Blast_Score=77, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17531443, Length=521, Percent_Identity=23.8003838771593, Blast_Score=139, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=563, Percent_Identity=25.044404973357, Blast_Score=135, Evalue=4e-32,
Organism=Caenorhabditis elegans, GI71994703, Length=563, Percent_Identity=25.044404973357, Blast_Score=135, Evalue=5e-32,
Organism=Caenorhabditis elegans, GI71994690, Length=563, Percent_Identity=25.044404973357, Blast_Score=135, Evalue=6e-32,
Organism=Caenorhabditis elegans, GI17558820, Length=504, Percent_Identity=25.1984126984127, Blast_Score=125, Evalue=4e-29,
Organism=Caenorhabditis elegans, GI32563687, Length=540, Percent_Identity=25, Blast_Score=125, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17557194, Length=539, Percent_Identity=22.0779220779221, Blast_Score=119, Evalue=6e-27,
Organism=Caenorhabditis elegans, GI17560308, Length=534, Percent_Identity=24.1573033707865, Blast_Score=116, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17559526, Length=368, Percent_Identity=25.8152173913043, Blast_Score=112, Evalue=6e-25,
Organism=Caenorhabditis elegans, GI17538037, Length=539, Percent_Identity=22.0779220779221, Blast_Score=110, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI71985884, Length=359, Percent_Identity=25.9052924791086, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI71996755, Length=510, Percent_Identity=23.5294117647059, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17551278, Length=537, Percent_Identity=23.463687150838, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17560140, Length=365, Percent_Identity=21.9178082191781, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6319699, Length=526, Percent_Identity=25.8555133079848, Blast_Score=139, Evalue=8e-34,
Organism=Drosophila melanogaster, GI18859661, Length=515, Percent_Identity=29.7087378640777, Blast_Score=157, Evalue=2e-38,
Organism=Drosophila melanogaster, GI24581924, Length=572, Percent_Identity=26.9230769230769, Blast_Score=151, Evalue=1e-36,
Organism=Drosophila melanogaster, GI21355181, Length=526, Percent_Identity=23.1939163498099, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24648257, Length=536, Percent_Identity=22.5746268656716, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24653035, Length=547, Percent_Identity=21.5722120658135, Blast_Score=89, Evalue=7e-18,
Organism=Drosophila melanogaster, GI281365686, Length=505, Percent_Identity=19.8019801980198, Blast_Score=88, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21358303, Length=529, Percent_Identity=20.9829867674858, Blast_Score=86, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24648253, Length=539, Percent_Identity=20.2226345083488, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21356947, Length=560, Percent_Identity=21.4285714285714, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24648260, Length=553, Percent_Identity=22.6039783001808, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21356441, Length=540, Percent_Identity=21.1111111111111, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24656500, Length=508, Percent_Identity=21.8503937007874, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI161076582, Length=558, Percent_Identity=21.505376344086, Blast_Score=76, Evalue=7e-14,
Organism=Drosophila melanogaster, GI20130357, Length=367, Percent_Identity=23.1607629427793, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 58998; Mature: 58998

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGIMQDWPLTVDKILEHARLQHGQREIITRRVEGNITRTTYSQLYDMSKQVSTALKDEG
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCC
IALGDRVATLGWNSERHMASWYGAMGIGAVLHTINPRLHPEQIAWIANHAEDKVLIFDKT
CEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEECH
FLPIVEAIKDKLETVKTFVIYAGADTMPENTLGAIAFDIWIDGRSKSVRWGDFPEDTACG
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCE
LCYTSGTTGNPKGVLYSHRSNVLHTLVTMGKDAMGMGAADSVMPVVPMFHANAWGLAMSC
EEEECCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEC
PATGANMVMPGAQMDGASIYELLDSEKVSLTAAVPTVWLMLLTHLQQNDLKLPYLKKVLI
CCCCCCEECCCCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GGSAIPEKILRAFEEEYEVEVIHAWGMTETSPLGTLGALPPHLEKAPVDARMKQKLKQGR
CCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
PPFGVELKLVDDDGEDVARDGQASGRLLVRGAAVAAGYFKGAGGHVLDADGWFDTGDVAT
CCCCEEEEEECCCCHHHHCCCCCCCEEEEEEHHHHHHHHCCCCCCEEECCCCCCCCCEEE
IDEIGTMTITDRAKDVIKSGGEWISSIDIENIAVGHPKVANAAAIGIYHPKWDERPLLIV
HHHCCCEEECHHHHHHHHCCCCEEHHCCCCCEECCCCCCCCEEEEEEECCCCCCCCEEEE
QAAAGETPTKEEILKSLEGKIAKWWTPDDVQFVDNIPLGATGKINKLALRETFKDYKLPT
EECCCCCCCHHHHHHHHCCCEEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC
A
C
>Mature Secondary Structure
MKGIMQDWPLTVDKILEHARLQHGQREIITRRVEGNITRTTYSQLYDMSKQVSTALKDEG
CCCCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHCC
IALGDRVATLGWNSERHMASWYGAMGIGAVLHTINPRLHPEQIAWIANHAEDKVLIFDKT
CEECCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCEEEEEECH
FLPIVEAIKDKLETVKTFVIYAGADTMPENTLGAIAFDIWIDGRSKSVRWGDFPEDTACG
HHHHHHHHHHHHHHHEEEEEEECCCCCCCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCE
LCYTSGTTGNPKGVLYSHRSNVLHTLVTMGKDAMGMGAADSVMPVVPMFHANAWGLAMSC
EEEECCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHCCCCCCEEEEC
PATGANMVMPGAQMDGASIYELLDSEKVSLTAAVPTVWLMLLTHLQQNDLKLPYLKKVLI
CCCCCCEECCCCCCCCHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
GGSAIPEKILRAFEEEYEVEVIHAWGMTETSPLGTLGALPPHLEKAPVDARMKQKLKQGR
CCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
PPFGVELKLVDDDGEDVARDGQASGRLLVRGAAVAAGYFKGAGGHVLDADGWFDTGDVAT
CCCCEEEEEECCCCHHHHCCCCCCCEEEEEEHHHHHHHHCCCCCCEEECCCCCCCCCEEE
IDEIGTMTITDRAKDVIKSGGEWISSIDIENIAVGHPKVANAAAIGIYHPKWDERPLLIV
HHHCCCEEECHHHHHHHHCCCCEEHHCCCCCEECCCCCCCCEEEEEEECCCCCCCCEEEE
QAAAGETPTKEEILKSLEGKIAKWWTPDDVQFVDNIPLGATGKINKLALRETFKDYKLPT
EECCCCCCCHHHHHHHHCCCEEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHCCCCC
A
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Coenzyme A; D-carnitine; Coenzyme A; crotono-betaine; L-carnitine [C]

Specific reaction: Coenzyme A + D-carnitine = D-carnitinyl-CoA Coenzyme A + crotono-betaine = crotonobetainyl-CoA Coenzyme A + L-carnitine = L-carnitinyl-CoA [C]

General reaction: Ligases; Forming Carbon-Sulfur Bonds; Acid-Thiol Ligases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]