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The map label for this gene is nuoI

Identifier: 114798298

GI number: 114798298

Start: 1794742

End: 1795227

Strand: Direct

Name: nuoI

Synonym: HNE_1753

Alternate gene names: 114798298

Gene position: 1794742-1795227 (Clockwise)

Preceding gene: 114799074

Following gene: 114797370

Centisome position: 48.44

GC content: 59.26

Gene sequence:

>486_bases
ATGAGCCTTGCGCAGACACTTCGCGGCGCGCTGCTGACAGATTTCATGGGCGCAGCCTGGATTGCGGTGCGGGAAATGTT
CCGGCGCAAGGCGACCGTGAATTACCCGTTTGAGAAAAACCCGCTTTCGCCGCGGTTCCGGGGCGAACATGTTCTGCGCC
GCTATCCGAGTGGCGAAGAGCGCTGCATTGCGTGCAAACTCTGCGAGGCGATCTGCCCCGCACAAGCGATTACGATTGAG
GCTGAGCCTCGCGAAGACGGGGCGCGCCGGACGACGCGGTACGACATTGACATGGTGAAATGCATCTATTGCGGCTTCTG
TCAGGAAGCCTGCCCGGTGGACGCCATCGTTGAAGGGCCAAACTTCGAGTTTGCAACAGAAACACGGGAGGAGCTGTTTT
ATGACAAGGAGCGCCTCCTCGCGAATGGCGACCGCTGGGAACGGCTGATTGCCAAGAATCTGGAATTGGATGCGCCGTAC
CGCTAA

Upstream 100 bases:

>100_bases
ATGATCAACTGATGCGTCTAGGCTGGAAAATCTTCCTGCCGACATCGCTGGCGGCGGTCATCATCGTGGCAGGTTATGTG
ACATTCGTGGGAGGTCTCTC

Downstream 100 bases:

>100_bases
GGGCGGCGCTGGGTTGAAGCAGGGCCAGCCCAATCGGGTTATGGCGTTTATCAGGAAGGGGCGGACAGGCCGTGGCGTTG
ATCGCTTTCTCATTGATTGC

Product: NADH dehydrogenase subunit I

Products: NA

Alternate protein names: NADH dehydrogenase I subunit I; NDH-1 subunit I

Number of amino acids: Translated: 161; Mature: 160

Protein sequence:

>161_residues
MSLAQTLRGALLTDFMGAAWIAVREMFRRKATVNYPFEKNPLSPRFRGEHVLRRYPSGEERCIACKLCEAICPAQAITIE
AEPREDGARRTTRYDIDMVKCIYCGFCQEACPVDAIVEGPNFEFATETREELFYDKERLLANGDRWERLIAKNLELDAPY
R

Sequences:

>Translated_161_residues
MSLAQTLRGALLTDFMGAAWIAVREMFRRKATVNYPFEKNPLSPRFRGEHVLRRYPSGEERCIACKLCEAICPAQAITIE
AEPREDGARRTTRYDIDMVKCIYCGFCQEACPVDAIVEGPNFEFATETREELFYDKERLLANGDRWERLIAKNLELDAPY
R
>Mature_160_residues
SLAQTLRGALLTDFMGAAWIAVREMFRRKATVNYPFEKNPLSPRFRGEHVLRRYPSGEERCIACKLCEAICPAQAITIEA
EPREDGARRTTRYDIDMVKCIYCGFCQEACPVDAIVEGPNFEFATETREELFYDKERLLANGDRWERLIAKNLELDAPYR

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1143

COG function: function code C; Formate hydrogenlyase subunit 6/NADH:ubiquinone oxidoreductase 23 kD subunit (chain I)

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains

Homologues:

Organism=Homo sapiens, GI4505371, Length=154, Percent_Identity=74.6753246753247, Blast_Score=245, Evalue=1e-65,
Organism=Escherichia coli, GI1788617, Length=143, Percent_Identity=39.1608391608392, Blast_Score=107, Evalue=3e-25,
Organism=Escherichia coli, GI1789075, Length=127, Percent_Identity=29.9212598425197, Blast_Score=62, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17555194, Length=159, Percent_Identity=69.1823899371069, Blast_Score=235, Evalue=7e-63,
Organism=Drosophila melanogaster, GI17864306, Length=153, Percent_Identity=75.1633986928105, Blast_Score=243, Evalue=4e-65,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOI_HYPNA (Q0C1D6)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760457.1
- ProteinModelPortal:   Q0C1D6
- SMR:   Q0C1D6
- STRING:   Q0C1D6
- GeneID:   4289462
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1753
- NMPDR:   fig|228405.5.peg.1688
- TIGR:   HNE_1753
- eggNOG:   COG1143
- HOGENOM:   HBG724359
- OMA:   HALKVSH
- PhylomeDB:   Q0C1D6
- ProtClustDB:   PRK05888
- BioCyc:   HNEP81032:HNE_1753-MONOMER
- HAMAP:   MF_01351
- InterPro:   IPR001450
- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR012285
- InterPro:   IPR010226
- Gene3D:   G3DSA:1.10.1060.10
- TIGRFAMs:   TIGR01971

Pfam domain/function: PF00037 Fer4

EC number: =1.6.99.5

Molecular weight: Translated: 18578; Mature: 18447

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00198 4FE4S_FER_1; PS51379 4FE4S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
5.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLAQTLRGALLTDFMGAAWIAVREMFRRKATVNYPFEKNPLSPRFRGEHVLRRYPSGEE
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCH
RCIACKLCEAICPAQAITIEAEPREDGARRTTRYDIDMVKCIYCGFCQEACPVDAIVEGP
HHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
NFEFATETREELFYDKERLLANGDRWERLIAKNLELDAPYR
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SLAQTLRGALLTDFMGAAWIAVREMFRRKATVNYPFEKNPLSPRFRGEHVLRRYPSGEE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCCCH
RCIACKLCEAICPAQAITIEAEPREDGARRTTRYDIDMVKCIYCGFCQEACPVDAIVEGP
HHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCEEECCC
NFEFATETREELFYDKERLLANGDRWERLIAKNLELDAPYR
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA