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The map label for this gene is prfC [H]

Identifier: 114798284

GI number: 114798284

Start: 758558

End: 760159

Strand: Direct

Name: prfC [H]

Synonym: HNE_0752

Alternate gene names: 114798284

Gene position: 758558-760159 (Clockwise)

Preceding gene: 114798497

Following gene: 114799024

Centisome position: 20.47

GC content: 62.73

Gene sequence:

>1602_bases
ATGTCGCACGTGACTGAAGCCGCCCGGCGGCGCACCTTTGCCATTATCTCCCACCCTGACGCGGGCAAGACGACGCTGAC
GGAGAACCTGCTCCTCGCAGGCGGCGCCATCCGGGCAGCCGGTGAAGTGGCTGCGCGCGGCGAAACCCGGCGCACACGGT
CTGACTGGATGAAGATCGAGCGTGACCGCGGCATCTCGGTTTCGGCTTCGGTGATGACGTTCGAGTTCGACGGCTACATG
TTCAACCTGCTCGACACGCCGGGCCACGAAGACTTCTCCGAAGATACCTACCGCACGCTGACCGCTGCCGACTGCGCCGT
CATGGTGCTCGACGCCGCCAAAGGCATTGAGCCCCAGACGCTGAAGCTGTTCGAAGTCTGCCGTATGCGGGACATCCCCA
TCGTCACCTTCATCAACAAGATGGACCGCGAAGCGCTTGAGCCCATCGCGCTGCTCGATGAGGTTCAGGACAAGCTGCAG
CTCGATACCTCGCCCCTCTACTGGCCCGCTGCCAGCGGCCAGCGCTTTGCCGGCATGCTCGACCTTGCCCGCAACCAGTT
CCTGCAATTCGAGCGCCGCCCTGGCGAACCTGCGCGCATCGGTCCGGCCACGGAAATTTCCGGCGAGTCTGATAGCCTGA
AAGCCAACCTCGACCCCTACGTCTTCGCCGAGATGACAGAAGGCATCGAAATGGCGCGCAGCCTGCTGCCGGAGTTTGAC
CCTGAAGCATTCCGTGCAGGCAATATGACGCCGGTCGTGTTCGGCTCGGCGCTGCGCTATTTCGGCGTGGCGGAGCTCCT
CCGCACGCTGCACTCTTACGCGCCCGCTCCCCGCCCACAGAAGGCGGTAAAGAAGGGCACCCCGGTATCCATCTCCCGCG
ATGACCCGTCCGTCAGCGGATTTGTCTTCAAGATCCAGGCGAACATGGACCCCAACCACCGGGACCGCGTGGCCTTCCTA
CGCCTCTGCTCAGGCGAATTCCGGCGCGGCATGCGCCTGAAGACCACCAGCGGCAAACAGCTCAACATCCACAATCCGCT
GATGTTCATGGCGCAGGACCGCGAGATTGCCGAGACCGCCTATGCGGGCGACGTGATCGGCGTGCCAAACCATGGCCAGT
TGCGCGTGGGCGACTCGCTTTCGGAGAATGGCGACATCCAGTTCGGCGGCATCCCGAACTTCGCGCCTGAGCTTCTCCGC
CGCGCCCGCGTCAAAGACCCGATGAAGGCCAAACACCTGCGCAAGGCGCTCGAAAGCCTCGCCGAAGAGGGCGTCACCCA
GCTGTTCCTGCCATCGATCGGCTCGGACATGATCGTCGGCGCAGTCGGCCAGCTTCAGTTTGAAGTGATGATCGAGCGGA
TGACGGCCGAATACAATCTCGAAGTCATGTTCGAGCAGGCGCCCTATAACGTCGCCCGCTGGATGGCGTGTGATGATCCC
AAAAAGCTCGAAGAGTTCCTTGACCGGAACAAATCAGCCTCGGGCACGGACCTTGACGGCGCGCCGGTCTATCTTGCGAA
GAATGCGTGGGATGTCGGCTATGCCCAGGAGAAGAACCCGGACATCCGCTTCACGGCAACGAAGGAACGGGCGCTGGCGT
AA

Upstream 100 bases:

>100_bases
GATTAAGGGCGAAGCCCTGATTTCCCCCGCCCGGGCAGCGAGCCCCTTCATTCCATTCGCCAAATCGGCTAGGGCCTCCG
GCGAAGAGAGAACCTGACCC

Downstream 100 bases:

>100_bases
CGCCGCCCTGCAACTGGCCAGGCTCGGGCACCGGCTGAGCTGCCGGCACCGGCGTTTCTCCCAGAGCTGCTTTTTTCAGC
GCCAGCGCAATGCCCGATGT

Product: putative peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MSHVTEAARRRTFAIISHPDAGKTTLTENLLLAGGAIRAAGEVAARGETRRTRSDWMKIERDRGISVSASVMTFEFDGYM
FNLLDTPGHEDFSEDTYRTLTAADCAVMVLDAAKGIEPQTLKLFEVCRMRDIPIVTFINKMDREALEPIALLDEVQDKLQ
LDTSPLYWPAASGQRFAGMLDLARNQFLQFERRPGEPARIGPATEISGESDSLKANLDPYVFAEMTEGIEMARSLLPEFD
PEAFRAGNMTPVVFGSALRYFGVAELLRTLHSYAPAPRPQKAVKKGTPVSISRDDPSVSGFVFKIQANMDPNHRDRVAFL
RLCSGEFRRGMRLKTTSGKQLNIHNPLMFMAQDREIAETAYAGDVIGVPNHGQLRVGDSLSENGDIQFGGIPNFAPELLR
RARVKDPMKAKHLRKALESLAEEGVTQLFLPSIGSDMIVGAVGQLQFEVMIERMTAEYNLEVMFEQAPYNVARWMACDDP
KKLEEFLDRNKSASGTDLDGAPVYLAKNAWDVGYAQEKNPDIRFTATKERALA

Sequences:

>Translated_533_residues
MSHVTEAARRRTFAIISHPDAGKTTLTENLLLAGGAIRAAGEVAARGETRRTRSDWMKIERDRGISVSASVMTFEFDGYM
FNLLDTPGHEDFSEDTYRTLTAADCAVMVLDAAKGIEPQTLKLFEVCRMRDIPIVTFINKMDREALEPIALLDEVQDKLQ
LDTSPLYWPAASGQRFAGMLDLARNQFLQFERRPGEPARIGPATEISGESDSLKANLDPYVFAEMTEGIEMARSLLPEFD
PEAFRAGNMTPVVFGSALRYFGVAELLRTLHSYAPAPRPQKAVKKGTPVSISRDDPSVSGFVFKIQANMDPNHRDRVAFL
RLCSGEFRRGMRLKTTSGKQLNIHNPLMFMAQDREIAETAYAGDVIGVPNHGQLRVGDSLSENGDIQFGGIPNFAPELLR
RARVKDPMKAKHLRKALESLAEEGVTQLFLPSIGSDMIVGAVGQLQFEVMIERMTAEYNLEVMFEQAPYNVARWMACDDP
KKLEEFLDRNKSASGTDLDGAPVYLAKNAWDVGYAQEKNPDIRFTATKERALA
>Mature_532_residues
SHVTEAARRRTFAIISHPDAGKTTLTENLLLAGGAIRAAGEVAARGETRRTRSDWMKIERDRGISVSASVMTFEFDGYMF
NLLDTPGHEDFSEDTYRTLTAADCAVMVLDAAKGIEPQTLKLFEVCRMRDIPIVTFINKMDREALEPIALLDEVQDKLQL
DTSPLYWPAASGQRFAGMLDLARNQFLQFERRPGEPARIGPATEISGESDSLKANLDPYVFAEMTEGIEMARSLLPEFDP
EAFRAGNMTPVVFGSALRYFGVAELLRTLHSYAPAPRPQKAVKKGTPVSISRDDPSVSGFVFKIQANMDPNHRDRVAFLR
LCSGEFRRGMRLKTTSGKQLNIHNPLMFMAQDREIAETAYAGDVIGVPNHGQLRVGDSLSENGDIQFGGIPNFAPELLRR
ARVKDPMKAKHLRKALESLAEEGVTQLFLPSIGSDMIVGAVGQLQFEVMIERMTAEYNLEVMFEQAPYNVARWMACDDPK
KLEEFLDRNKSASGTDLDGAPVYLAKNAWDVGYAQEKNPDIRFTATKERALA

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI19923640, Length=535, Percent_Identity=26.1682242990654, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI18390331, Length=482, Percent_Identity=25.103734439834, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI25306283, Length=354, Percent_Identity=29.9435028248588, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI25306287, Length=293, Percent_Identity=32.7645051194539, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI157426893, Length=142, Percent_Identity=33.8028169014084, Blast_Score=92, Evalue=1e-18,
Organism=Homo sapiens, GI94966754, Length=145, Percent_Identity=31.7241379310345, Blast_Score=84, Evalue=5e-16,
Organism=Homo sapiens, GI4503483, Length=154, Percent_Identity=31.8181818181818, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI310132016, Length=113, Percent_Identity=32.7433628318584, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310110807, Length=113, Percent_Identity=32.7433628318584, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI310123363, Length=113, Percent_Identity=32.7433628318584, Blast_Score=70, Evalue=3e-12,
Organism=Escherichia coli, GI1790835, Length=530, Percent_Identity=46.4150943396226, Blast_Score=504, Evalue=1e-144,
Organism=Escherichia coli, GI1789738, Length=502, Percent_Identity=25.4980079681275, Blast_Score=143, Evalue=3e-35,
Organism=Escherichia coli, GI1788922, Length=169, Percent_Identity=35.5029585798817, Blast_Score=100, Evalue=3e-22,
Organism=Escherichia coli, GI48994988, Length=171, Percent_Identity=32.7485380116959, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17533571, Length=516, Percent_Identity=27.5193798449612, Blast_Score=159, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI17556745, Length=488, Percent_Identity=24.3852459016393, Blast_Score=127, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=35.6060606060606, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=165, Percent_Identity=32.1212121212121, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71988819, Length=141, Percent_Identity=29.7872340425532, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71988811, Length=137, Percent_Identity=29.9270072992701, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323098, Length=482, Percent_Identity=26.5560165975104, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6322359, Length=417, Percent_Identity=27.5779376498801, Blast_Score=123, Evalue=9e-29,
Organism=Saccharomyces cerevisiae, GI6323320, Length=157, Percent_Identity=28.6624203821656, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=153, Percent_Identity=33.9869281045752, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=153, Percent_Identity=33.9869281045752, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24582462, Length=490, Percent_Identity=27.1428571428571, Blast_Score=145, Evalue=1e-34,
Organism=Drosophila melanogaster, GI221458488, Length=508, Percent_Identity=25.3937007874016, Blast_Score=143, Evalue=3e-34,
Organism=Drosophila melanogaster, GI78706572, Length=157, Percent_Identity=36.3057324840764, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24585709, Length=177, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24585711, Length=177, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24585713, Length=177, Percent_Identity=33.3333333333333, Blast_Score=84, Evalue=2e-16,
Organism=Drosophila melanogaster, GI28574573, Length=146, Percent_Identity=31.5068493150685, Blast_Score=68, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21357743, Length=143, Percent_Identity=31.4685314685315, Blast_Score=68, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 59267; Mature: 59136

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHVTEAARRRTFAIISHPDAGKTTLTENLLLAGGAIRAAGEVAARGETRRTRSDWMKIE
CCHHHHHHHCCEEEEEECCCCCCHHHHHHHHEECCCHHHCCHHHHCCCCHHHHHHHHHHH
RDRGISVSASVMTFEFDGYMFNLLDTPGHEDFSEDTYRTLTAADCAVMVLDAAKGIEPQT
HCCCCEEEEEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEHHHCCCCCHH
LKLFEVCRMRDIPIVTFINKMDREALEPIALLDEVQDKLQLDTSPLYWPAASGQRFAGML
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHH
DLARNQFLQFERRPGEPARIGPATEISGESDSLKANLDPYVFAEMTEGIEMARSLLPEFD
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCC
PEAFRAGNMTPVVFGSALRYFGVAELLRTLHSYAPAPRPQKAVKKGTPVSISRDDPSVSG
CHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEECCCCCCCE
FVFKIQANMDPNHRDRVAFLRLCSGEFRRGMRLKTTSGKQLNIHNPLMFMAQDREIAETA
EEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCEEECCCCEEEEECCCHHHHHH
YAGDVIGVPNHGQLRVGDSLSENGDIQFGGIPNFAPELLRRARVKDPMKAKHLRKALESL
HCCCEEECCCCCCEEECCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
AEEGVTQLFLPSIGSDMIVGAVGQLQFEVMIERMTAEYNLEVMFEQAPYNVARWMACDDP
HHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHEEECCCH
KKLEEFLDRNKSASGTDLDGAPVYLAKNAWDVGYAQEKNPDIRFTATKERALA
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECHHHCCC
>Mature Secondary Structure 
SHVTEAARRRTFAIISHPDAGKTTLTENLLLAGGAIRAAGEVAARGETRRTRSDWMKIE
CHHHHHHHCCEEEEEECCCCCCHHHHHHHHEECCCHHHCCHHHHCCCCHHHHHHHHHHH
RDRGISVSASVMTFEFDGYMFNLLDTPGHEDFSEDTYRTLTAADCAVMVLDAAKGIEPQT
HCCCCEEEEEEEEEEECCEEEEEECCCCCCCCCCHHHHHHHHHHHHEEEEHHHCCCCCHH
LKLFEVCRMRDIPIVTFINKMDREALEPIALLDEVQDKLQLDTSPLYWPAASGQRFAGML
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCHHHHHH
DLARNQFLQFERRPGEPARIGPATEISGESDSLKANLDPYVFAEMTEGIEMARSLLPEFD
HHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCC
PEAFRAGNMTPVVFGSALRYFGVAELLRTLHSYAPAPRPQKAVKKGTPVSISRDDPSVSG
CHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCEEEECCCCCCCE
FVFKIQANMDPNHRDRVAFLRLCSGEFRRGMRLKTTSGKQLNIHNPLMFMAQDREIAETA
EEEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEECCCCEEECCCCEEEEECCCHHHHHH
YAGDVIGVPNHGQLRVGDSLSENGDIQFGGIPNFAPELLRRARVKDPMKAKHLRKALESL
HCCCEEECCCCCCEEECCCCCCCCCEEECCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHH
AEEGVTQLFLPSIGSDMIVGAVGQLQFEVMIERMTAEYNLEVMFEQAPYNVARWMACDDP
HHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCHHHEEECCCH
KKLEEFLDRNKSASGTDLDGAPVYLAKNAWDVGYAQEKNPDIRFTATKERALA
HHHHHHHHCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCCEEEEECHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA