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The map label for this gene is pnpA

Identifier: 114798182

GI number: 114798182

Start: 3579558

End: 3581699

Strand: Reverse

Name: pnpA

Synonym: HNE_3441

Alternate gene names: 114798182

Gene position: 3581699-3579558 (Counterclockwise)

Preceding gene: 114797457

Following gene: 114800418

Centisome position: 96.67

GC content: 60.74

Gene sequence:

>2142_bases
ATGTTTAACAAGCAATCCGTGTCGCTGGAATGGGCTGGCCGCACGCTGACAATCGAGACGGGCCAAGTGGCACGTCAGGC
CGATGGCGCCGTCATGGTGCAGTACGGCGATACCATTGTCCTCGCGACCGCCGTGTTCGCGAAGGAAGCAAAACCGGGTC
AGGACTTCTTCCCCCTGACCGTGAATTACCAGGAAAAGTACTTCGCCTCCGGCCGGATTCCCGGCGGATTCTTCAAGCGC
GAAGGCCGCCCCACCGAGAAAGAGACGCTGACCTCGCGCCTCATCGACCGTCCGATCCGTCCGCTGTTCGTCGACGGTTT
CAAGCATGAAGTCCAGGTCGTCGTCACGACGCTGTCCTATGACCTCGAGAATGATGCCGACATCATCGCGCTCGTCGGCG
CCTCTGCGGCGCTGGTGCTTTCCGGCGCGCCCTTCATGGGCCCGATCGGCGCTGCGCGCGTTGGCTACAAAGACGGCCAG
TACATCATCAACCCGACGATTGCCGAACTTGAAGAGTCCGAGCTTGACCTCGTGGTTGCCGGCACCACCGACGCCGTGAT
GATGGTTGAATCTCAGGCGGCTGAGCTTTCCGAAGACGTGATGCTCGGCGCTGTTGTCGCCGGTCATGACGCGATGCAGC
CGGTGATCGATGCGATCATTGCGCTGGCAGAAAAAGCGGCGAAAGAGCCGTTTGCTTATGAGCCGCCAGACCATTCGGCT
GCCCTCAAATCGGTGATCGACACTGTCGGCGCCGATCTGTCGAAAGCCTACAAGATCACCGCCAAGGGCGAGCGTTATGC
TGCCATCGGCGCGGCCAAGGACAAGGCGAAAGCTGCCCTTCTTGGTACGGATGAAGCCCCCGGCGTGATGACGCCGGAAG
TCTTCAAGACCGTGTTCAAGGAAGCGGAAGCTTCTGTTGTGCGCGGCGACATCCTGAAAACCGGTCAGCGGATCGACGGG
CGCAAGCTCGACCAGATCCGCCCGATCGTTGCCGAAGCCGGCTTCCTGCCGCGCACGCATGGTTCGTCGCTGTTCACGCG
CGGCGAGACGCAGGCGATCTGCGTTGCCACGCTCGGAACGTCCGATGACGAACAGTACATCGACGGTCTGGACGGCACGA
AAAAAGAAAAGTTCATGCTGCACTACAACTTCCCGCCCTATTCGGTCGGGGAGACGGGCCGCATGGGCGGCGCAGGCCGC
CGCGAGATCGGCCACGGCAAACTTGCCTGGCGCGCGCTCAAGGCTGTTCTGCCCAAGCATGAAGACTTTCCGTACACGAT
CCGTATGGTCTCCGAGATCACCGAGTCGAACGGGTCTTCCTCGATGGCGACTGTTTGCGGTTGCTCGCTGGCCATGATGG
ATGCGGGCGTTCCGCTGACCCGTCCGGTCTCGGGCATTGCCATGGGCCTCATCCTCGAAGGCTCCGAGTTTGCGGTTCTC
TCCGACATCCTCGGCGACGAAGACCACCTCGGCGACATGGACTTCAAGGTTGCCGGTACTGAAAACGGCGTGACCTCGCT
CCAGATGGACATCAAGGTTGCGGGCATCACCAAGGACATCATGGGCAAAGCCCTTGAGCAGGCCAAAGGCGGCCGGATGC
ACATCCTCGGTGAAATGGGCAAGGCCCTGACCGCTTCGCGCGGCCAGCTGTCGGAAAACGCGCCGCAGATGGAAATCATC
AAGGTGCCGACCGACAAGATCCGTGATGTGATCGGTTCGGGCGGCAAGGTCATCCGCGGCATCGTCGACGAGACGGGCGC
CAAGGTGAACATCGATGACGATGGCACGGTTCAGATTTCGGCGATGGATCGCAAGTCGATCGATGCCGCGATCAAGATGA
TCAAGGGCATCACGGCGGAAGCCGAAGTCGGCGAGATCTACGAAGGCAAAGTCGTTTCCATGAAGGACTTCGGTATCTTC
GTGAACTTCTTCGGCCCCAAGGACGGCCTCGTCCACGTCTCGCAGATGGCCAACAAGCGCATTGGCCACCCCAAGGAGAT
GGTCAAGGAAGGCGACAAAGTGTGGGTGAAGCTCATGGGCTTCGATGAGCGCGGCAAGGTCCGCCTCTCCATGAAGGTCG
TTGACCAGGAGACCGGCAAGGAGCTTGCTGAAGAAGCGGGCGACGACGCTTCGGAAGACTAG

Upstream 100 bases:

>100_bases
AGCTGATCACGGAACTCGGCATCCGCCGCTAGGTCCGAAAAACAAGCCCGCCGGGCAATAGGGCTCTGGCGGGCTTTTCG
TATGTGAAAGAAAGACTGAA

Downstream 100 bases:

>100_bases
TCTTCCCTCCGATTATCAAATGAATGAGGGCCGCTCCTTGATGGGCGGCCCTTTTTCATGGAAACACCGGCTACAGAAGA
GGGCGCGCTGTCATGACAAA

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MFNKQSVSLEWAGRTLTIETGQVARQADGAVMVQYGDTIVLATAVFAKEAKPGQDFFPLTVNYQEKYFASGRIPGGFFKR
EGRPTEKETLTSRLIDRPIRPLFVDGFKHEVQVVVTTLSYDLENDADIIALVGASAALVLSGAPFMGPIGAARVGYKDGQ
YIINPTIAELEESELDLVVAGTTDAVMMVESQAAELSEDVMLGAVVAGHDAMQPVIDAIIALAEKAAKEPFAYEPPDHSA
ALKSVIDTVGADLSKAYKITAKGERYAAIGAAKDKAKAALLGTDEAPGVMTPEVFKTVFKEAEASVVRGDILKTGQRIDG
RKLDQIRPIVAEAGFLPRTHGSSLFTRGETQAICVATLGTSDDEQYIDGLDGTKKEKFMLHYNFPPYSVGETGRMGGAGR
REIGHGKLAWRALKAVLPKHEDFPYTIRMVSEITESNGSSSMATVCGCSLAMMDAGVPLTRPVSGIAMGLILEGSEFAVL
SDILGDEDHLGDMDFKVAGTENGVTSLQMDIKVAGITKDIMGKALEQAKGGRMHILGEMGKALTASRGQLSENAPQMEII
KVPTDKIRDVIGSGGKVIRGIVDETGAKVNIDDDGTVQISAMDRKSIDAAIKMIKGITAEAEVGEIYEGKVVSMKDFGIF
VNFFGPKDGLVHVSQMANKRIGHPKEMVKEGDKVWVKLMGFDERGKVRLSMKVVDQETGKELAEEAGDDASED

Sequences:

>Translated_713_residues
MFNKQSVSLEWAGRTLTIETGQVARQADGAVMVQYGDTIVLATAVFAKEAKPGQDFFPLTVNYQEKYFASGRIPGGFFKR
EGRPTEKETLTSRLIDRPIRPLFVDGFKHEVQVVVTTLSYDLENDADIIALVGASAALVLSGAPFMGPIGAARVGYKDGQ
YIINPTIAELEESELDLVVAGTTDAVMMVESQAAELSEDVMLGAVVAGHDAMQPVIDAIIALAEKAAKEPFAYEPPDHSA
ALKSVIDTVGADLSKAYKITAKGERYAAIGAAKDKAKAALLGTDEAPGVMTPEVFKTVFKEAEASVVRGDILKTGQRIDG
RKLDQIRPIVAEAGFLPRTHGSSLFTRGETQAICVATLGTSDDEQYIDGLDGTKKEKFMLHYNFPPYSVGETGRMGGAGR
REIGHGKLAWRALKAVLPKHEDFPYTIRMVSEITESNGSSSMATVCGCSLAMMDAGVPLTRPVSGIAMGLILEGSEFAVL
SDILGDEDHLGDMDFKVAGTENGVTSLQMDIKVAGITKDIMGKALEQAKGGRMHILGEMGKALTASRGQLSENAPQMEII
KVPTDKIRDVIGSGGKVIRGIVDETGAKVNIDDDGTVQISAMDRKSIDAAIKMIKGITAEAEVGEIYEGKVVSMKDFGIF
VNFFGPKDGLVHVSQMANKRIGHPKEMVKEGDKVWVKLMGFDERGKVRLSMKVVDQETGKELAEEAGDDASED
>Mature_713_residues
MFNKQSVSLEWAGRTLTIETGQVARQADGAVMVQYGDTIVLATAVFAKEAKPGQDFFPLTVNYQEKYFASGRIPGGFFKR
EGRPTEKETLTSRLIDRPIRPLFVDGFKHEVQVVVTTLSYDLENDADIIALVGASAALVLSGAPFMGPIGAARVGYKDGQ
YIINPTIAELEESELDLVVAGTTDAVMMVESQAAELSEDVMLGAVVAGHDAMQPVIDAIIALAEKAAKEPFAYEPPDHSA
ALKSVIDTVGADLSKAYKITAKGERYAAIGAAKDKAKAALLGTDEAPGVMTPEVFKTVFKEAEASVVRGDILKTGQRIDG
RKLDQIRPIVAEAGFLPRTHGSSLFTRGETQAICVATLGTSDDEQYIDGLDGTKKEKFMLHYNFPPYSVGETGRMGGAGR
REIGHGKLAWRALKAVLPKHEDFPYTIRMVSEITESNGSSSMATVCGCSLAMMDAGVPLTRPVSGIAMGLILEGSEFAVL
SDILGDEDHLGDMDFKVAGTENGVTSLQMDIKVAGITKDIMGKALEQAKGGRMHILGEMGKALTASRGQLSENAPQMEII
KVPTDKIRDVIGSGGKVIRGIVDETGAKVNIDDDGTVQISAMDRKSIDAAIKMIKGITAEAEVGEIYEGKVVSMKDFGIF
VNFFGPKDGLVHVSQMANKRIGHPKEMVKEGDKVWVKLMGFDERGKVRLSMKVVDQETGKELAEEAGDDASED

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=725, Percent_Identity=36.9655172413793, Blast_Score=442, Evalue=1e-124,
Organism=Homo sapiens, GI21361576, Length=81, Percent_Identity=49.3827160493827, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI145693187, Length=693, Percent_Identity=56.1327561327561, Blast_Score=763, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=730, Percent_Identity=33.1506849315069, Blast_Score=361, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17535281, Length=91, Percent_Identity=41.7582417582418, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6320850, Length=109, Percent_Identity=33.0275229357798, Blast_Score=77, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281362905, Length=715, Percent_Identity=37.4825174825175, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651641, Length=715, Percent_Identity=37.4825174825175, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI24651643, Length=715, Percent_Identity=37.4825174825175, Blast_Score=451, Evalue=1e-127,
Organism=Drosophila melanogaster, GI161079377, Length=666, Percent_Identity=36.6366366366366, Blast_Score=404, Evalue=1e-112,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_HYPNA (Q0BWM9)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_762114.1
- ProteinModelPortal:   Q0BWM9
- STRING:   Q0BWM9
- GeneID:   4288000
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_3441
- NMPDR:   fig|228405.5.peg.3295
- TIGR:   HNE_3441
- eggNOG:   COG1185
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- PhylomeDB:   Q0BWM9
- ProtClustDB:   PRK11824
- BioCyc:   HNEP81032:HNE_3441-MONOMER
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 76423; Mature: 76423

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNKQSVSLEWAGRTLTIETGQVARQADGAVMVQYGDTIVLATAVFAKEAKPGQDFFPLT
CCCCCEEEEEECCEEEEEECCHHHHHCCCEEEEEECCEEEEEEHHHHHCCCCCCCEEEEE
VNYQEKYFASGRIPGGFFKREGRPTEKETLTSRLIDRPIRPLFVDGFKHEVQVVVTTLSY
EECCHHEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEEEEE
DLENDADIIALVGASAALVLSGAPFMGPIGAARVGYKDGQYIINPTIAELEESELDLVVA
CCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCEEECCHHHHCCCCCCCEEEE
GTTDAVMMVESQAAELSEDVMLGAVVAGHDAMQPVIDAIIALAEKAAKEPFAYEPPDHSA
CCCCEEEEEEHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
ALKSVIDTVGADLSKAYKITAKGERYAAIGAAKDKAKAALLGTDEAPGVMTPEVFKTVFK
HHHHHHHHHCCCCHHEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHH
EAEASVVRGDILKTGQRIDGRKLDQIRPIVAEAGFLPRTHGSSLFTRGETQAICVATLGT
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEEEECC
SDDEQYIDGLDGTKKEKFMLHYNFPPYSVGETGRMGGAGRREIGHGKLAWRALKAVLPKH
CCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
EDFPYTIRMVSEITESNGSSSMATVCGCSLAMMDAGVPLTRPVSGIAMGLILEGSEFAVL
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHH
SDILGDEDHLGDMDFKVAGTENGVTSLQMDIKVAGITKDIMGKALEQAKGGRMHILGEMG
HHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEHHHHH
KALTASRGQLSENAPQMEIIKVPTDKIRDVIGSGGKVIRGIVDETGAKVNIDDDGTVQIS
HHHHCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCEEHHHHHCCCCEEEECCCCEEEEE
AMDRKSIDAAIKMIKGITAEAEVGEIYEGKVVSMKDFGIFVNFFGPKDGLVHVSQMANKR
ECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECEEEEEEECCCCCCEEHHHHHHHC
IGHPKEMVKEGDKVWVKLMGFDERGKVRLSMKVVDQETGKELAEEAGDDASED
CCCHHHHHCCCCEEEEEEEECCCCCCEEEEEEEECHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MFNKQSVSLEWAGRTLTIETGQVARQADGAVMVQYGDTIVLATAVFAKEAKPGQDFFPLT
CCCCCEEEEEECCEEEEEECCHHHHHCCCEEEEEECCEEEEEEHHHHHCCCCCCCEEEEE
VNYQEKYFASGRIPGGFFKREGRPTEKETLTSRLIDRPIRPLFVDGFKHEVQVVVTTLSY
EECCHHEEECCCCCCCHHCCCCCCCHHHHHHHHHHHCCCCCEEECCCCCEEEEEEEEEEE
DLENDADIIALVGASAALVLSGAPFMGPIGAARVGYKDGQYIINPTIAELEESELDLVVA
CCCCCCCEEEEECCCEEEEEECCCCCCCCCCCCCCCCCCCEEECCHHHHCCCCCCCEEEE
GTTDAVMMVESQAAELSEDVMLGAVVAGHDAMQPVIDAIIALAEKAAKEPFAYEPPDHSA
CCCCEEEEEEHHHHHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHH
ALKSVIDTVGADLSKAYKITAKGERYAAIGAAKDKAKAALLGTDEAPGVMTPEVFKTVFK
HHHHHHHHHCCCCHHEEEEEECCCEEEEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHH
EAEASVVRGDILKTGQRIDGRKLDQIRPIVAEAGFLPRTHGSSLFTRGETQAICVATLGT
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCEEECCCCCEEEEEEECC
SDDEQYIDGLDGTKKEKFMLHYNFPPYSVGETGRMGGAGRREIGHGKLAWRALKAVLPKH
CCCHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCC
EDFPYTIRMVSEITESNGSSSMATVCGCSLAMMDAGVPLTRPVSGIAMGLILEGSEFAVL
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEECCCCHHHH
SDILGDEDHLGDMDFKVAGTENGVTSLQMDIKVAGITKDIMGKALEQAKGGRMHILGEMG
HHHCCCCCCCCCCCEEEECCCCCCEEEEEEEEEECCCHHHHHHHHHHCCCCEEEEHHHHH
KALTASRGQLSENAPQMEIIKVPTDKIRDVIGSGGKVIRGIVDETGAKVNIDDDGTVQIS
HHHHCCCCCCCCCCCCEEEEECCHHHHHHHHCCCCCEEHHHHHCCCCEEEECCCCEEEEE
AMDRKSIDAAIKMIKGITAEAEVGEIYEGKVVSMKDFGIFVNFFGPKDGLVHVSQMANKR
ECCHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECEEEEEEECCCCCCEEHHHHHHHC
IGHPKEMVKEGDKVWVKLMGFDERGKVRLSMKVVDQETGKELAEEAGDDASED
CCCHHHHHCCCCEEEEEEEECCCCCCEEEEEEEECHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA