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The map label for this gene is mscL

Identifier: 114798146

GI number: 114798146

Start: 176661

End: 177074

Strand: Reverse

Name: mscL

Synonym: HNE_0190

Alternate gene names: 114798146

Gene position: 177074-176661 (Counterclockwise)

Preceding gene: 114798899

Following gene: 114800197

Centisome position: 4.78

GC content: 57.97

Gene sequence:

>414_bases
ATGTTTAAAGAATTCAAAGAGTTCGCCCTCAAGGGCAATCTTGTCGACCTCGCCGTCGGCTTCATCCTCGGCGGCGCCTT
CGGCACGATCGTAACGTCGTTGGTGAACGACATCATGATGCCGCCGCTGGGCATGCTGATGGGCGGGGTAGATTTCGCCG
ATCTGTTCATTTCGTTGAACGGCACGGAGTATGTCTCCCTGGTGGCCGCCACGGAAGCCGGCGCCCCGGTGATCGCCTAT
GGCAAGTTCATCAATGCCGTGATTTCCTTCGTCATCATGGCGCTGGCCCTGTTCTTCGTGATCAAGGGCATGAACACGCT
GAAGAAGAAGGAAGAAGCGGCGCCGCCTCCGGCCCCACCGCGCCAGGAAGTGCTTCTGGAAGAAATCCGCAACCTTCTGG
CCAAGAACCAGTAG

Upstream 100 bases:

>100_bases
CCTTTTCGCGCCCTTGTTGCAGCGCCTTGACGCTGATCTCAAACACCGCGATTTCTGTTGCATACGCTGGCGGCATCCCG
CCAATCGGAAGGGGTAGATA

Downstream 100 bases:

>100_bases
GCAAAGCTGCGGCACAGGGTTCGCAAAGTATTAAGCAGTCCGCTGCTACTTTGTGGATAGTAGAGTACCGCGTAGAGTGC
GGAAGGGCGGCCTGTCGGGT

Product: large-conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 137; Mature: 137

Protein sequence:

>137_residues
MFKEFKEFALKGNLVDLAVGFILGGAFGTIVTSLVNDIMMPPLGMLMGGVDFADLFISLNGTEYVSLVAATEAGAPVIAY
GKFINAVISFVIMALALFFVIKGMNTLKKKEEAAPPPAPPRQEVLLEEIRNLLAKNQ

Sequences:

>Translated_137_residues
MFKEFKEFALKGNLVDLAVGFILGGAFGTIVTSLVNDIMMPPLGMLMGGVDFADLFISLNGTEYVSLVAATEAGAPVIAY
GKFINAVISFVIMALALFFVIKGMNTLKKKEEAAPPPAPPRQEVLLEEIRNLLAKNQ
>Mature_137_residues
MFKEFKEFALKGNLVDLAVGFILGGAFGTIVTSLVNDIMMPPLGMLMGGVDFADLFISLNGTEYVSLVAATEAGAPVIAY
GKFINAVISFVIMALALFFVIKGMNTLKKKEEAAPPPAPPRQEVLLEEIRNLLAKNQ

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family

Homologues:

Organism=Escherichia coli, GI1789686, Length=138, Percent_Identity=51.4492753623188, Blast_Score=131, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MSCL_HYPNA (Q0C5R9)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_758924.1
- ProteinModelPortal:   Q0C5R9
- SMR:   Q0C5R9
- STRING:   Q0C5R9
- GeneID:   4290379
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_0190
- NMPDR:   fig|228405.5.peg.188
- TIGR:   HNE_0190
- eggNOG:   COG1970
- HOGENOM:   HBG727604
- OMA:   IKATRCP
- PhylomeDB:   Q0C5R9
- ProtClustDB:   PRK13952
- BioCyc:   HNEP81032:HNE_0190-MONOMER
- HAMAP:   MF_00115
- InterPro:   IPR001185
- PRINTS:   PR01264
- TIGRFAMs:   TIGR00220

Pfam domain/function: PF01741 MscL; SSF81330 MS_channel

EC number: NA

Molecular weight: Translated: 14723; Mature: 14723

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS01327 MSCL

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x104590c8)-; HASH(0xfe215f0)-;

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKEFKEFALKGNLVDLAVGFILGGAFGTIVTSLVNDIMMPPLGMLMGGVDFADLFISLN
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCC
GTEYVSLVAATEAGAPVIAYGKFINAVISFVIMALALFFVIKGMNTLKKKEEAAPPPAPP
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCC
RQEVLLEEIRNLLAKNQ
HHHHHHHHHHHHHCCCH
>Mature Secondary Structure
MFKEFKEFALKGNLVDLAVGFILGGAFGTIVTSLVNDIMMPPLGMLMGGVDFADLFISLN
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCCHHHHHHHHCC
GTEYVSLVAATEAGAPVIAYGKFINAVISFVIMALALFFVIKGMNTLKKKEEAAPPPAPP
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCCCC
RQEVLLEEIRNLLAKNQ
HHHHHHHHHHHHHCCCH

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA