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The map label for this gene is menE [H]

Identifier: 114797975

GI number: 114797975

Start: 2148908

End: 2150155

Strand: Direct

Name: menE [H]

Synonym: HNE_2075

Alternate gene names: 114797975

Gene position: 2148908-2150155 (Clockwise)

Preceding gene: 114799754

Following gene: 114798063

Centisome position: 58.0

GC content: 62.66

Gene sequence:

>1248_bases
GTGGCGCACGCCAGCGCCATTCTGAGCCGTGGTGACTTTGCCACGGCGGCGGAAGCCCTGACAGCAAAGATGACGGCGGC
GGGCGTCTCACGCCTGATGGTGCATTCAGACAATCCGTTCGATATTCTGCGCGCCATCGACGCCGCAAACCGCACGGGCG
CTGACCTCTTTATTGCCCACACCAACCTTCCCGAAACGCATATCGATGCGGTGGTGAAGAAGTTCGGTGTGCAGATGATT
GTGGCAGCCAATGACCGCGCAACGGAAGAAAGCGCCGTAGGGGCTGCTTCCGGGCTTATCTACATGATGACCTCAGGCAC
CACCGGCGCACCCAAGATTGCCTCGCACAGCCTGAACGCCCTGATGAGCCGCGCCAAGGCGGCGATGCACCCAGGAAACC
GCGGCGCAAAATGGCTGCTCACCTACCAGCCGACAGGCTTTGCCGGTGTGCAGGTCCAGTTGACGGCTGTGGTTGGACGG
GGCGTCGTTGTGGCGCCCGAACAGCGCACGCCGGCAGGATTTCTGGAAGCGGCAAAGGCGAATGGCGTTACGCAGATTTC
GGCCACGCCGACCTTCTGGCGGGCCTTCCTTATGGTGATCCGGCCAGGCGATCTGAACCTGCGCCAGATTACGCTGGGCG
GCGAAGCAGCCGACCAGTCGACGCTCGACCGGATCAAGAAAGCCTTCCCCGAAGCGCGCGTCACGCATATCTATGCCTCG
ACCGAAGCGGGCGTTGTTTTCTCCGTACACGATGGACGCGAAGGCTTCCCCGCCGAATGGCTTGAGACCGAGACGCAGGG
CGTCACGATGCGCGTCAGCGATGGATTCCTGCACATTCGCACGCCCAACGCGATGGGCGGGTATGTGTCGGACACCGCGC
AGCCAAAACTGGACGATGGCTGGCTGGCAACGGCTGACCGCGTTGAAATCCGAGGGGATCGCGCCTTCATCGTCGGCCGC
GACGACAGCACGATCAATGTTGCCGGCTCCAAGGTTTATCCGCTGATGGTGGAGCAGTTCCTGCTGCGCCAGGCGGGCGT
CATCGAGGCGCACGTCTACGGCGTTTCCAATCCGATCAGCGGCCAGCTAGTCGCCGCTGATGTCGTCATCGAACCGGGGC
TCGACCCCGCCGCGACCCGCAGCGCCATTCTCGCTGCCTGCCGCGAAAACCTCGCTCAATATCAGGTTCCGCGCGCCTTC
AAGGTGGTTGACGCCATCGCTGTGCGTGAATCTGGCAAGAAAGGCTAA

Upstream 100 bases:

>100_bases
GGTTCATCGAGTTCCGCACGGTCGGCGAACTGGCGAAGCTTTACGCAAAATGAGTGGTGCGGGCGCCTTCGGTCTTGAGG
CAGGCGACGCGACACCTCTG

Downstream 100 bases:

>100_bases
TACGCATGACCGCGCCCGCAGCTTCTTCCCAAATGCGTCACGTTATCGTCACCGGCGGCAGCCGGGGACTGGGCGCCGCC
ATGATCGACGGACTGCTTGC

Product: AMP-binding domain-containing protein

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 415; Mature: 414

Protein sequence:

>415_residues
MAHASAILSRGDFATAAEALTAKMTAAGVSRLMVHSDNPFDILRAIDAANRTGADLFIAHTNLPETHIDAVVKKFGVQMI
VAANDRATEESAVGAASGLIYMMTSGTTGAPKIASHSLNALMSRAKAAMHPGNRGAKWLLTYQPTGFAGVQVQLTAVVGR
GVVVAPEQRTPAGFLEAAKANGVTQISATPTFWRAFLMVIRPGDLNLRQITLGGEAADQSTLDRIKKAFPEARVTHIYAS
TEAGVVFSVHDGREGFPAEWLETETQGVTMRVSDGFLHIRTPNAMGGYVSDTAQPKLDDGWLATADRVEIRGDRAFIVGR
DDSTINVAGSKVYPLMVEQFLLRQAGVIEAHVYGVSNPISGQLVAADVVIEPGLDPAATRSAILAACRENLAQYQVPRAF
KVVDAIAVRESGKKG

Sequences:

>Translated_415_residues
MAHASAILSRGDFATAAEALTAKMTAAGVSRLMVHSDNPFDILRAIDAANRTGADLFIAHTNLPETHIDAVVKKFGVQMI
VAANDRATEESAVGAASGLIYMMTSGTTGAPKIASHSLNALMSRAKAAMHPGNRGAKWLLTYQPTGFAGVQVQLTAVVGR
GVVVAPEQRTPAGFLEAAKANGVTQISATPTFWRAFLMVIRPGDLNLRQITLGGEAADQSTLDRIKKAFPEARVTHIYAS
TEAGVVFSVHDGREGFPAEWLETETQGVTMRVSDGFLHIRTPNAMGGYVSDTAQPKLDDGWLATADRVEIRGDRAFIVGR
DDSTINVAGSKVYPLMVEQFLLRQAGVIEAHVYGVSNPISGQLVAADVVIEPGLDPAATRSAILAACRENLAQYQVPRAF
KVVDAIAVRESGKKG
>Mature_414_residues
AHASAILSRGDFATAAEALTAKMTAAGVSRLMVHSDNPFDILRAIDAANRTGADLFIAHTNLPETHIDAVVKKFGVQMIV
AANDRATEESAVGAASGLIYMMTSGTTGAPKIASHSLNALMSRAKAAMHPGNRGAKWLLTYQPTGFAGVQVQLTAVVGRG
VVVAPEQRTPAGFLEAAKANGVTQISATPTFWRAFLMVIRPGDLNLRQITLGGEAADQSTLDRIKKAFPEARVTHIYAST
EAGVVFSVHDGREGFPAEWLETETQGVTMRVSDGFLHIRTPNAMGGYVSDTAQPKLDDGWLATADRVEIRGDRAFIVGRD
DSTINVAGSKVYPLMVEQFLLRQAGVIEAHVYGVSNPISGQLVAADVVIEPGLDPAATRSAILAACRENLAQYQVPRAFK
VVDAIAVRESGKKG

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788107, Length=337, Percent_Identity=25.2225519287834, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24581924, Length=338, Percent_Identity=27.2189349112426, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR010192
- InterPro:   IPR023015 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 44031; Mature: 43899

Theoretical pI: Translated: 7.39; Mature: 7.39

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHASAILSRGDFATAAEALTAKMTAAGVSRLMVHSDNPFDILRAIDAANRTGADLFIAH
CCCHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCCCCCEEEEEE
TNLPETHIDAVVKKFGVQMIVAANDRATEESAVGAASGLIYMMTSGTTGAPKIASHSLNA
CCCCHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
LMSRAKAAMHPGNRGAKWLLTYQPTGFAGVQVQLTAVVGRGVVVAPEQRTPAGFLEAAKA
HHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHC
NGVTQISATPTFWRAFLMVIRPGDLNLRQITLGGEAADQSTLDRIKKAFPEARVTHIYAS
CCEEEEECCHHHHHEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEE
TEAGVVFSVHDGREGFPAEWLETETQGVTMRVSDGFLHIRTPNAMGGYVSDTAQPKLDDG
CCCCEEEEECCCCCCCCHHHHCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCC
WLATADRVEIRGDRAFIVGRDDSTINVAGSKVYPLMVEQFLLRQAGVIEAHVYGVSNPIS
CEECCCEEEEECCEEEEEECCCCEEEECCCEEHHHHHHHHHHHHCCEEEEEEEECCCCCC
GQLVAADVVIEPGLDPAATRSAILAACRENLAQYQVPRAFKVVDAIAVRESGKKG
CCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AHASAILSRGDFATAAEALTAKMTAAGVSRLMVHSDNPFDILRAIDAANRTGADLFIAH
CCHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHHHCCCCCCCEEEEEE
TNLPETHIDAVVKKFGVQMIVAANDRATEESAVGAASGLIYMMTSGTTGAPKIASHSLNA
CCCCHHHHHHHHHHCCEEEEEEECCCCCHHHHHHHCCCEEEEEECCCCCCCHHHHHHHHH
LMSRAKAAMHPGNRGAKWLLTYQPTGFAGVQVQLTAVVGRGVVVAPEQRTPAGFLEAAKA
HHHHHHHHCCCCCCCCEEEEEECCCCCCCEEEEEEEEECCCEEEECCCCCCHHHHHHHHC
NGVTQISATPTFWRAFLMVIRPGDLNLRQITLGGEAADQSTLDRIKKAFPEARVTHIYAS
CCEEEEECCHHHHHEEEEEECCCCCCEEEEEECCCCCCHHHHHHHHHHCCCCEEEEEEEE
TEAGVVFSVHDGREGFPAEWLETETQGVTMRVSDGFLHIRTPNAMGGYVSDTAQPKLDDG
CCCCEEEEECCCCCCCCHHHHCCCCCCEEEEEECCEEEEECCCCCCCCCCCCCCCCCCCC
WLATADRVEIRGDRAFIVGRDDSTINVAGSKVYPLMVEQFLLRQAGVIEAHVYGVSNPIS
CEECCCEEEEECCEEEEEECCCCEEEECCCEEHHHHHHHHHHHHCCEEEEEEEECCCCCC
GQLVAADVVIEPGLDPAATRSAILAACRENLAQYQVPRAFKVVDAIAVRESGKKG
CCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA