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The map label for this gene is trmD [H]

Identifier: 114797793

GI number: 114797793

Start: 37728

End: 38483

Strand: Direct

Name: trmD [H]

Synonym: HNE_0045

Alternate gene names: 114797793

Gene position: 37728-38483 (Clockwise)

Preceding gene: 114799732

Following gene: 114797917

Centisome position: 1.02

GC content: 63.1

Gene sequence:

>756_bases
ATGTTCAAGGCCACAGCCATCACCCTCATACCGGAAGCCTGGCCGGGTCCCCTCGGCGCCTCCATTCTGGGCCGGGCGCG
GGCCGAAGGCATCTGGGATCTCGAAACGGTCGATCTGCGCACCTTCGGTCTCGGCAAGCATCGCAAGGTGGACGATACGC
CCGCTGGCGGCGGGGCCGGCCTCGTCCTGCGGCCCGACGTGGCTGCGGCGGCGGTCGATTCTGTCCAGAAGTGTCCTGAT
TTGTCCCGATCTGTCCCGGCAATGACGCGCCCTGTCGTGTATTTGTCCCCGCGGGGGCGCCGGTTTGACCAGGAAATGGC
AAGGCAGTGGGCGGCCGGTCCGGGCGTCATTCTTTTTTGCGGAAGGTTCGAGGGGCTCGATGAACGCGCCATAGAAGCGC
GGGACATGCAGGAAGTTTCCCTCGGAGATTTTGTCCTGGCGGGCGGTGATGTCGCAGCCATGGCCATGCTGGAGGCCGCG
ATCCGTCTCCTCCCCGGTGTGGCGGGCAATTCTGCTTCCATTACAGGGGAATCCTTCGAGAACGGCCTCCTCGAATACCC
CCATTATACGCTCCCCAGAGAGTGGGAAGGGCGTCAGACCCCCCCTGTTTTGCTGTCTGGCGACCACAGGCGGATAGAAG
AGTGGCGAATCAGCCGCTCAAAGGCGTTGACTTTTGACCGCCGCCCCGATTTATACGCCGCTTACTCCGAAACCCCGACC
CCTACTGCGCCGGAGACTGGCGATGAACCTGATTAA

Upstream 100 bases:

>100_bases
AATGGCGGCGGGGCTGTTCCACTGAACGCGCGCAAGGCCGAACCCGATGCCGAGCCTGGCGGTGGGTGACGGACGGCCCG
GCCCGCGCTAAACCCCGCCC

Downstream 100 bases:

>100_bases
AGAGCTTGAGACCGCTGAAATCGCGCGCGTACTCGGCGACAAAACCATTCCGCAATTCTCGCCCGGCGATACCGTCGCCG
TGAACGTGAAGATCAAGGAA

Product: tRNA (guanine-N1)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 251; Mature: 251

Protein sequence:

>251_residues
MFKATAITLIPEAWPGPLGASILGRARAEGIWDLETVDLRTFGLGKHRKVDDTPAGGGAGLVLRPDVAAAAVDSVQKCPD
LSRSVPAMTRPVVYLSPRGRRFDQEMARQWAAGPGVILFCGRFEGLDERAIEARDMQEVSLGDFVLAGGDVAAMAMLEAA
IRLLPGVAGNSASITGESFENGLLEYPHYTLPREWEGRQTPPVLLSGDHRRIEEWRISRSKALTFDRRPDLYAAYSETPT
PTAPETGDEPD

Sequences:

>Translated_251_residues
MFKATAITLIPEAWPGPLGASILGRARAEGIWDLETVDLRTFGLGKHRKVDDTPAGGGAGLVLRPDVAAAAVDSVQKCPD
LSRSVPAMTRPVVYLSPRGRRFDQEMARQWAAGPGVILFCGRFEGLDERAIEARDMQEVSLGDFVLAGGDVAAMAMLEAA
IRLLPGVAGNSASITGESFENGLLEYPHYTLPREWEGRQTPPVLLSGDHRRIEEWRISRSKALTFDRRPDLYAAYSETPT
PTAPETGDEPD
>Mature_251_residues
MFKATAITLIPEAWPGPLGASILGRARAEGIWDLETVDLRTFGLGKHRKVDDTPAGGGAGLVLRPDVAAAAVDSVQKCPD
LSRSVPAMTRPVVYLSPRGRRFDQEMARQWAAGPGVILFCGRFEGLDERAIEARDMQEVSLGDFVLAGGDVAAMAMLEAA
IRLLPGVAGNSASITGESFENGLLEYPHYTLPREWEGRQTPPVLLSGDHRRIEEWRISRSKALTFDRRPDLYAAYSETPT
PTAPETGDEPD

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=225, Percent_Identity=39.5555555555556, Blast_Score=170, Evalue=7e-44,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 27242; Mature: 27242

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKATAITLIPEAWPGPLGASILGRARAEGIWDLETVDLRTFGLGKHRKVDDTPAGGGAG
CCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCC
LVLRPDVAAAAVDSVQKCPDLSRSVPAMTRPVVYLSPRGRRFDQEMARQWAAGPGVILFC
EEECCCHHHHHHHHHHHCCCCHHCCCHHHCCEEEECCCCCCHHHHHHHHHCCCCCEEEEE
GRFEGLDERAIEARDMQEVSLGDFVLAGGDVAAMAMLEAAIRLLPGVAGNSASITGESFE
CCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCEECCCHHH
NGLLEYPHYTLPREWEGRQTPPVLLSGDHRRIEEWRISRSKALTFDRRPDLYAAYSETPT
CCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHEEEECCCCCEEEEECCCCC
PTAPETGDEPD
CCCCCCCCCCC
>Mature Secondary Structure
MFKATAITLIPEAWPGPLGASILGRARAEGIWDLETVDLRTFGLGKHRKVDDTPAGGGAG
CCCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCC
LVLRPDVAAAAVDSVQKCPDLSRSVPAMTRPVVYLSPRGRRFDQEMARQWAAGPGVILFC
EEECCCHHHHHHHHHHHCCCCHHCCCHHHCCEEEECCCCCCHHHHHHHHHCCCCCEEEEE
GRFEGLDERAIEARDMQEVSLGDFVLAGGDVAAMAMLEAAIRLLPGVAGNSASITGESFE
CCCCCCCHHHHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCEECCCHHH
NGLLEYPHYTLPREWEGRQTPPVLLSGDHRRIEEWRISRSKALTFDRRPDLYAAYSETPT
CCCCCCCCCCCCCCCCCCCCCCEEEECCCHHHHHHHHHHHHEEEECCCCCEEEEECCCCC
PTAPETGDEPD
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA