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The map label for this gene is prpB [C]

Identifier: 114797772

GI number: 114797772

Start: 2881130

End: 2881906

Strand: Direct

Name: prpB [C]

Synonym: HNE_2718

Alternate gene names: 114797772

Gene position: 2881130-2881906 (Clockwise)

Preceding gene: 114799308

Following gene: 114799925

Centisome position: 77.76

GC content: 69.63

Gene sequence:

>777_bases
ATGCCCACCCAGACCGAGAAACTTGCCCAATTTGCCAGCCTCCATGGCGGCGCCCAGCCTCTCGTGCTGGTGAACATCTG
GGACGCGGGGTCTGCCCGCACAGTGGCCGCTGCGGGCGCCCGCGCGCTGGCGACGGGCAGCGCCTCGGTGGGCGGGGCGC
TCGGCTTTGCCGATGGCGAGGCTGTGCCGCTCGATCTGGTGCTCGATCATGCCGCGCGGATCGTGGCGGCGGTAGACCTT
CCCGTCTCGCTCGACTTTGAAGCCGGATATGCCGCATCGGCAGACGGCGTGGCGGCCAACATCCGCCGCGTTGTGGGCAC
AGGCGCTGTCGGCATCAACCTTGAGGATGGCTATCCCGCCGGCGATGGCGAGGGGGTGCGCGCTATCGGAGACGCCACCG
CACGCATCGAGGCTGCGCGGGCCGCCGCCGACAGCCTCCTGCCCGGCTTCTGGATCAACGCCCGCACCGACATCTGCCTG
CGCGCCAAGGCCGAGGACCACGCCGCCCATATCAATGACGTGATCGCCCGCGGCAAAGCCTATGCCGAGGCGGGCGCCAG
CAGCTTCTTTGTGCCCGGCCTGCGGGATATGGCGCTGATTGCAAAGGTCTGCGCGGCGTGCCCGCTGCCGGTGAATGTGA
TGGCCGGGCCGGAGGCAGGCGGCTTTGGCGCGCTGGCAGAGGCGGGCGTGCGCCGTATCAGCTATGGCCCCTTTCCGTGG
CGCGCGGCGATGGCAACGCTCACTTCTTTTGCAGAGCGCGCCGCCAAGGCAGGCTGA

Upstream 100 bases:

>100_bases
CCCGGACGCGCAAGAGACGCGCAAGAATCGGGTCGCAGGTGGCGCGGGCCTCTGGCATCCCCCGCCGCAACACATATGCC
AGCTCAAGAGGACGCCCGAC

Downstream 100 bases:

>100_bases
CGTGCGGCAGCCCTTGCCGCGCCTCAGAATCCGGGCTAGCTGACCGTCATATATTCTCTTCCGGGAGAGAGCGGCATTCG
GTAGCCGCCGCCGAAGGCGC

Product: hypothetical protein

Products: Pyruvate; Succinate [C]

Alternate protein names: NA

Number of amino acids: Translated: 258; Mature: 257

Protein sequence:

>258_residues
MPTQTEKLAQFASLHGGAQPLVLVNIWDAGSARTVAAAGARALATGSASVGGALGFADGEAVPLDLVLDHAARIVAAVDL
PVSLDFEAGYAASADGVAANIRRVVGTGAVGINLEDGYPAGDGEGVRAIGDATARIEAARAAADSLLPGFWINARTDICL
RAKAEDHAAHINDVIARGKAYAEAGASSFFVPGLRDMALIAKVCAACPLPVNVMAGPEAGGFGALAEAGVRRISYGPFPW
RAAMATLTSFAERAAKAG

Sequences:

>Translated_258_residues
MPTQTEKLAQFASLHGGAQPLVLVNIWDAGSARTVAAAGARALATGSASVGGALGFADGEAVPLDLVLDHAARIVAAVDL
PVSLDFEAGYAASADGVAANIRRVVGTGAVGINLEDGYPAGDGEGVRAIGDATARIEAARAAADSLLPGFWINARTDICL
RAKAEDHAAHINDVIARGKAYAEAGASSFFVPGLRDMALIAKVCAACPLPVNVMAGPEAGGFGALAEAGVRRISYGPFPW
RAAMATLTSFAERAAKAG
>Mature_257_residues
PTQTEKLAQFASLHGGAQPLVLVNIWDAGSARTVAAAGARALATGSASVGGALGFADGEAVPLDLVLDHAARIVAAVDLP
VSLDFEAGYAASADGVAANIRRVVGTGAVGINLEDGYPAGDGEGVRAIGDATARIEAARAAADSLLPGFWINARTDICLR
AKAEDHAAHINDVIARGKAYAEAGASSFFVPGLRDMALIAKVCAACPLPVNVMAGPEAGGFGALAEAGVRRISYGPFPWR
AAMATLTSFAERAAKAG

Specific function: Catalyzes The Formation Of Pyruvate And Succinate From 2-Methylisocitrate (By Similarity). [C]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786525, Length=197, Percent_Identity=30.9644670050761, Blast_Score=68, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR015813 [H]

Pfam domain/function: NA

EC number: 4.1.3.30 [C]

Molecular weight: Translated: 25977; Mature: 25845

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPTQTEKLAQFASLHGGAQPLVLVNIWDAGSARTVAAAGARALATGSASVGGALGFADGE
CCCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHCCCHHEECCCCCCCCEEECCCCC
AVPLDLVLDHAARIVAAVDLPVSLDFEAGYAASADGVAANIRRVVGTGAVGINLEDGYPA
CCCHHHHHHHHHHHHEEECCCEEECCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCC
GDGEGVRAIGDATARIEAARAAADSLLPGFWINARTDICLRAKAEDHAAHINDVIARGKA
CCCCCEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHCHH
YAEAGASSFFVPGLRDMALIAKVCAACPLPVNVMAGPEAGGFGALAEAGVRRISYGPFPW
HHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCH
RAAMATLTSFAERAAKAG
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PTQTEKLAQFASLHGGAQPLVLVNIWDAGSARTVAAAGARALATGSASVGGALGFADGE
CCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHCCCHHEECCCCCCCCEEECCCCC
AVPLDLVLDHAARIVAAVDLPVSLDFEAGYAASADGVAANIRRVVGTGAVGINLEDGYPA
CCCHHHHHHHHHHHHEEECCCEEECCCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCC
GDGEGVRAIGDATARIEAARAAADSLLPGFWINARTDICLRAKAEDHAAHINDVIARGKA
CCCCCEEECCCHHHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCHHHHHHHHHHHHCHH
YAEAGASSFFVPGLRDMALIAKVCAACPLPVNVMAGPEAGGFGALAEAGVRRISYGPFPW
HHHCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCCCH
RAAMATLTSFAERAAKAG
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: methylisocitrate [C]

Specific reaction: methylisocitrate <==> Pyruvate + Succinate [C]

General reaction: Elimination [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]