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The map label for this gene is ycjG [H]

Identifier: 114797771

GI number: 114797771

Start: 3080011

End: 3081009

Strand: Direct

Name: ycjG [H]

Synonym: HNE_2922

Alternate gene names: 114797771

Gene position: 3080011-3081009 (Clockwise)

Preceding gene: 114798372

Following gene: 114797058

Centisome position: 83.13

GC content: 67.37

Gene sequence:

>999_bases
ATGGGTCATCTCCGGCTTGAAACTGATCACATTTCCTCGCCGTTGAGGGCAGCCTTTGCAATTTCGCGGGGATCGAAGAC
CAGCGCCGAGACGGTGCGGGTGCGCCTTTCAGAAGATGGAAACGCCGGACGGGGGGAAAGCGTTCCCTATGGACGGTATG
GCGAAACGGTACAGAGCGTACTCGAAGAGATCGACGCTGTGCGCGTCCAGATCGAGGCGGGGATCACGCGCGAGGCGCTG
CAAAGCCTGATGAAGCCGGGCGCAGCACGCTGCGCGGTGGATTGCGCGCTGTGGGATCTGGAGGCGAAGAAGACGGGAAA
GCCGGTCTGGCAACTGGCGGGGTTGCCTGAGCCTGCGCCAGTGGAGACGGCCCTCACGATCAGCCTGGATACGCCGGACG
CCATGGCGGCCGCAGCGACCGCAGCGCCGGGACGGCTGTTGAAGCTGAAGCTCGGCGGGGCGGAGGATCTGGCGCGGATC
AAGGCGGTGCATGAAGCCCGGCCTGAGGCGCGCCTGATTGCCGACGCCAATGAAGGGTTGAGCGAAGCCGACCTGCCAGG
GCTGGTGAAAGCCGCCGCAAGGCTCGGCGTGGTGCTGATTGAGCAACCCTTCCCTGCCGGGAAAGACGCGGCGCTGATGC
GGCGGCCGGGCGCGGTGGCGATCTGCGCCGACGAAAGCGCGCATACCAGCGCCGATATCCAGACACTGGCGAGGAATTAT
GACGCGGTGAACGTGAAGCTCGACAAGGCAGGCGGGCTGACCGAAGCGCTGGCGATGGTGCGCGCGGCGCGGACTGCCGG
GATGGGCGTGATGGTCGGCTGTATGGTGGCGGGGTCGCTCTCGATGGCGCCGGCCGTGCTGCTGGCGCAGCTGGCGGACG
CGGCAGATCTCGACGGACCGCTCTGGCTGGCGAGCGATATTGAGGGTGGGCTGGTGTATGCGGAGGGGCGGGTGTCGCCG
CCGGGGGCCGGGCTTTGGGGGTGGGGTGGCTCCCGGTAG

Upstream 100 bases:

>100_bases
AGGTTCAGCATTCTTTAGGGTCCTGACTGACCTGACTACCAAAACATGCTTAAGGACGGTGTGTGCCAATCCAATACAAT
TCACTTTGCAGGATTAACCC

Downstream 100 bases:

>100_bases
GGCGCAAGGGCGGTGAGAAGGTCCCGGATCGCGGCTTTGCCGCGTCCGGGATGACCCGGGTGGGTGTAGGTGGTTTTTTT
GGGCAGCAGGTTTGGCCGGA

Product: mandelate racemase/muconate lactonizing enzyme family protein

Products: 3-chloro-cis,cis-muconate

Alternate protein names: AE epimerase; AEE [H]

Number of amino acids: Translated: 332; Mature: 331

Protein sequence:

>332_residues
MGHLRLETDHISSPLRAAFAISRGSKTSAETVRVRLSEDGNAGRGESVPYGRYGETVQSVLEEIDAVRVQIEAGITREAL
QSLMKPGAARCAVDCALWDLEAKKTGKPVWQLAGLPEPAPVETALTISLDTPDAMAAAATAAPGRLLKLKLGGAEDLARI
KAVHEARPEARLIADANEGLSEADLPGLVKAAARLGVVLIEQPFPAGKDAALMRRPGAVAICADESAHTSADIQTLARNY
DAVNVKLDKAGGLTEALAMVRAARTAGMGVMVGCMVAGSLSMAPAVLLAQLADAADLDGPLWLASDIEGGLVYAEGRVSP
PGAGLWGWGGSR

Sequences:

>Translated_332_residues
MGHLRLETDHISSPLRAAFAISRGSKTSAETVRVRLSEDGNAGRGESVPYGRYGETVQSVLEEIDAVRVQIEAGITREAL
QSLMKPGAARCAVDCALWDLEAKKTGKPVWQLAGLPEPAPVETALTISLDTPDAMAAAATAAPGRLLKLKLGGAEDLARI
KAVHEARPEARLIADANEGLSEADLPGLVKAAARLGVVLIEQPFPAGKDAALMRRPGAVAICADESAHTSADIQTLARNY
DAVNVKLDKAGGLTEALAMVRAARTAGMGVMVGCMVAGSLSMAPAVLLAQLADAADLDGPLWLASDIEGGLVYAEGRVSP
PGAGLWGWGGSR
>Mature_331_residues
GHLRLETDHISSPLRAAFAISRGSKTSAETVRVRLSEDGNAGRGESVPYGRYGETVQSVLEEIDAVRVQIEAGITREALQ
SLMKPGAARCAVDCALWDLEAKKTGKPVWQLAGLPEPAPVETALTISLDTPDAMAAAATAAPGRLLKLKLGGAEDLARIK
AVHEARPEARLIADANEGLSEADLPGLVKAAARLGVVLIEQPFPAGKDAALMRRPGAVAICADESAHTSADIQTLARNYD
AVNVKLDKAGGLTEALAMVRAARTAGMGVMVGCMVAGSLSMAPAVLLAQLADAADLDGPLWLASDIEGGLVYAEGRVSPP
GAGLWGWGGSR

Specific function: Catalyzes the epimerization of L-Ala-D-Glu to L-Ala-L- Glu and has a role in the recycling of the murein peptide, of which L-Ala-D-Glu is a component. Is also able to catalyze the reverse reaction and the epimerization of all the L-Ala-X dipeptides, excep

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI87081876, Length=308, Percent_Identity=44.8051948051948, Blast_Score=236, Evalue=1e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018110
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: 5.5.1.7

Molecular weight: Translated: 34366; Mature: 34235

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGHLRLETDHISSPLRAAFAISRGSKTSAETVRVRLSEDGNAGRGESVPYGRYGETVQSV
CCCEEECHHHCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
LEEIDAVRVQIEAGITREALQSLMKPGAARCAVDCALWDLEAKKTGKPVWQLAGLPEPAP
HHHHHHEEEEEECCCCHHHHHHHHCCCCHHHEEEHHEEECCHHHCCCCCEEECCCCCCCC
VETALTISLDTPDAMAAAATAAPGRLLKLKLGGAEDLARIKAVHEARPEARLIADANEGL
CCEEEEEEECCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCC
SEADLPGLVKAAARLGVVLIEQPFPAGKDAALMRRPGAVAICADESAHTSADIQTLARNY
CCCCCHHHHHHHHHCCEEEEECCCCCCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHCCC
DAVNVKLDKAGGLTEALAMVRAARTAGMGVMVGCMVAGSLSMAPAVLLAQLADAADLDGP
CEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCC
LWLASDIEGGLVYAEGRVSPPGAGLWGWGGSR
EEEECCCCCCEEEECCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GHLRLETDHISSPLRAAFAISRGSKTSAETVRVRLSEDGNAGRGESVPYGRYGETVQSV
CCEEECHHHCCCHHHHHHHHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
LEEIDAVRVQIEAGITREALQSLMKPGAARCAVDCALWDLEAKKTGKPVWQLAGLPEPAP
HHHHHHEEEEEECCCCHHHHHHHHCCCCHHHEEEHHEEECCHHHCCCCCEEECCCCCCCC
VETALTISLDTPDAMAAAATAAPGRLLKLKLGGAEDLARIKAVHEARPEARLIADANEGL
CCEEEEEEECCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEECCCCCC
SEADLPGLVKAAARLGVVLIEQPFPAGKDAALMRRPGAVAICADESAHTSADIQTLARNY
CCCCCHHHHHHHHHCCEEEEECCCCCCCCHHHHCCCCEEEEECCCCCCCHHHHHHHHCCC
DAVNVKLDKAGGLTEALAMVRAARTAGMGVMVGCMVAGSLSMAPAVLLAQLADAADLDGP
CEEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCC
LWLASDIEGGLVYAEGRVSPPGAGLWGWGGSR
EEEECCCCCCEEEECCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2-chloro-2,5-dihydro-5-oxofuran-2-acetate

Specific reaction: 2-chloro-2,5-dihydro-5-oxofuran-2-acetate = 3-chloro-cis,cis-muconate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7499381; 9097039; 9278503 [H]