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The map label for this gene is fcs2 [H]

Identifier: 114797749

GI number: 114797749

Start: 882196

End: 884064

Strand: Direct

Name: fcs2 [H]

Synonym: HNE_0864

Alternate gene names: 114797749

Gene position: 882196-884064 (Clockwise)

Preceding gene: 114799838

Following gene: 114798777

Centisome position: 23.81

GC content: 64.15

Gene sequence:

>1869_bases
ATGAGCAAGACCGCGCATATTCCGTTTCGTGAGGTGCCTTATCTTCCGCTGAAGCTGAAGATCGACCGGCGCCCCGATGG
CACGATCTATCTCGACAGTGGCCATCCGCTCAAAGCCCATCCGCCGCATATGCTCGCTCCGCTGGTGAAATGGGGCGCCG
AGCGCCCGGACCAGACCTGGCTGGCCGAGCGCTGGCCGGAAAACCCCGCTGCCGATGACTGGCGCCGCATGACCTATGCC
GATGGCCTTGCCGCCGTCCGGCGCATTGCCGCCGCCTTCCTCAGCGAGGGCGCCGGGCAGGACGCGCCGGTGATGATCCT
CTCGAAGAATTCCATCGACAATGCGCTCGTCATGTACGGCGCCATGTGGGCGGCGAGCCCGGTGGTGCCGGTGACGCCCG
CCTACGCGACGCTCAGCCAGGACCTCCTGCGGTTCCGGTATATCGACCAGCTCACCACACCGAAATTCATCTATGTCGAA
GATGGCAACGAGTATCAGCGCGCCCTCGACAGCCTCGACCTGACCGGGCGTCTGGTGATCTATTCTCGCAATGCGCCGAA
AGTCCCCCGCGCCGTCAGTCTGGAGGATTTTGCCGGACAGACCGGCCCGGCGGTGGACGAGGCTTATGCCCGCCTCACGC
CGAAAACCGTCGCCAAATACATGCTCACCTCCGGCTCCACCGGGGAGCCCAAGGCCGTCATCAACACCCATGGCATGGTC
GCGGCCAATTCCAAGATGATCCGCTCGGTATGGGACGAGGAACGCCTCGACGCGATTACCGGCGGCCCGCAGGTGATGGT
GAACTTCCTGCCCTGGAGCCACACTTACGGCACCCATTCGATCCTCCACAACATGCTGGACTGGGGCGGCACGATGTATA
TCTGCCAGGGCGCGCCCACGCCCGCCCGCCTTCCGGAGATGATCCGTAACCTGAAGGAAATCCCCACCACCCAGCACACC
ACCGTGCCCCAGGCATGGGCGGCGCTGGCCACCGCGCTGGAGCAGGACGACGCCCTCGCCGAAGTGTTCTTCTCCCGCCT
CACCTCCATGGCCTATGGCGGCGCCTCAATGGGGCAGGACATCTACGAACGCATTCAGGAAGTCGCCGTCCGTGTCACCG
GCGAGCGCATCTCTCTGTCTGCCGGGTACGGCGCAACCGAAACCTCTCCCACCGCCTCAAATGTCCACTGGCCGAACGAC
AAGATGGGCCTCATCGGCCTGCCGCTGCCCGGCAACACATTCAAGCTGGTGCCAGCGGGCGACAAGCTGGAGCTGCGCGT
CAAAGGCGTCAACGTCACACCCGGCTATTTCCGCAACGCGGAGAAGACCGCCGAAGCCTTTGATGAAGAGGGCTATTACA
AGCTCGGCGACGCGGTGAAGTTCGTTGATCCGGCCGAGCCTGAACGCGGCCTCGCCTTTGATGGCCGCACGGTGGAAGAG
TTCAAGCTCGCCAGCGGCACATGGGTCTCGGCCGGCGCCGTGCGCGTTGCGGCCGTGGCGGCGTGTGGCGGCGCGCTGTC
AGACGCGGTTGTCTGCGGCCTCAACAAGGATGAGATCGGCCTTCTGGGCTTTCTCAACCTCGCCTGGTGTCAGCGCCTCG
TGGGCGAGGCCTTGCCTGCCGATCAGCTGGTCACCCATCCCAAGGTGCTCGAAGCCGTCAAGGCGGGGCTGAGCGCCCAC
AACAAGGCCCACCCCAATGCCTCTGCCCGCATTACGCGGATACTGCTGCAACCTGTCGAGCCGCGCGCTGACCTCGGCGA
GATCACCGAAAAAGGCTACATCAATCAGTCCCGGACGCAGGCCCTGCGCCCCGATCAACTTGATCTGCTATTTGCCGCCG
AAAATGCCGCCGGGAAAATCCTGATTTAA

Upstream 100 bases:

>100_bases
GGAGGGGCTTGCCCTTTCCGGCCGCCTGCGCGAGCAATCATGTCAGAAAACAAATTCATGGCGGGCCTGCCCTGCCATGC
GGGGAGGATAAATTTTGCCA

Downstream 100 bases:

>100_bases
TGGCATTTTCAGTGCCAATATCAGCCGGTTATGCCGAATCCTGCCTTTTTGGGGCTCGATTCCTTATCCAAAGCGCTTAA
ACATGCCCCTGAAGTCTTCG

Product: feruloyl-CoA synthase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase; Acyl-CoA synthetase [H]

Number of amino acids: Translated: 622; Mature: 621

Protein sequence:

>622_residues
MSKTAHIPFREVPYLPLKLKIDRRPDGTIYLDSGHPLKAHPPHMLAPLVKWGAERPDQTWLAERWPENPAADDWRRMTYA
DGLAAVRRIAAAFLSEGAGQDAPVMILSKNSIDNALVMYGAMWAASPVVPVTPAYATLSQDLLRFRYIDQLTTPKFIYVE
DGNEYQRALDSLDLTGRLVIYSRNAPKVPRAVSLEDFAGQTGPAVDEAYARLTPKTVAKYMLTSGSTGEPKAVINTHGMV
AANSKMIRSVWDEERLDAITGGPQVMVNFLPWSHTYGTHSILHNMLDWGGTMYICQGAPTPARLPEMIRNLKEIPTTQHT
TVPQAWAALATALEQDDALAEVFFSRLTSMAYGGASMGQDIYERIQEVAVRVTGERISLSAGYGATETSPTASNVHWPND
KMGLIGLPLPGNTFKLVPAGDKLELRVKGVNVTPGYFRNAEKTAEAFDEEGYYKLGDAVKFVDPAEPERGLAFDGRTVEE
FKLASGTWVSAGAVRVAAVAACGGALSDAVVCGLNKDEIGLLGFLNLAWCQRLVGEALPADQLVTHPKVLEAVKAGLSAH
NKAHPNASARITRILLQPVEPRADLGEITEKGYINQSRTQALRPDQLDLLFAAENAAGKILI

Sequences:

>Translated_622_residues
MSKTAHIPFREVPYLPLKLKIDRRPDGTIYLDSGHPLKAHPPHMLAPLVKWGAERPDQTWLAERWPENPAADDWRRMTYA
DGLAAVRRIAAAFLSEGAGQDAPVMILSKNSIDNALVMYGAMWAASPVVPVTPAYATLSQDLLRFRYIDQLTTPKFIYVE
DGNEYQRALDSLDLTGRLVIYSRNAPKVPRAVSLEDFAGQTGPAVDEAYARLTPKTVAKYMLTSGSTGEPKAVINTHGMV
AANSKMIRSVWDEERLDAITGGPQVMVNFLPWSHTYGTHSILHNMLDWGGTMYICQGAPTPARLPEMIRNLKEIPTTQHT
TVPQAWAALATALEQDDALAEVFFSRLTSMAYGGASMGQDIYERIQEVAVRVTGERISLSAGYGATETSPTASNVHWPND
KMGLIGLPLPGNTFKLVPAGDKLELRVKGVNVTPGYFRNAEKTAEAFDEEGYYKLGDAVKFVDPAEPERGLAFDGRTVEE
FKLASGTWVSAGAVRVAAVAACGGALSDAVVCGLNKDEIGLLGFLNLAWCQRLVGEALPADQLVTHPKVLEAVKAGLSAH
NKAHPNASARITRILLQPVEPRADLGEITEKGYINQSRTQALRPDQLDLLFAAENAAGKILI
>Mature_621_residues
SKTAHIPFREVPYLPLKLKIDRRPDGTIYLDSGHPLKAHPPHMLAPLVKWGAERPDQTWLAERWPENPAADDWRRMTYAD
GLAAVRRIAAAFLSEGAGQDAPVMILSKNSIDNALVMYGAMWAASPVVPVTPAYATLSQDLLRFRYIDQLTTPKFIYVED
GNEYQRALDSLDLTGRLVIYSRNAPKVPRAVSLEDFAGQTGPAVDEAYARLTPKTVAKYMLTSGSTGEPKAVINTHGMVA
ANSKMIRSVWDEERLDAITGGPQVMVNFLPWSHTYGTHSILHNMLDWGGTMYICQGAPTPARLPEMIRNLKEIPTTQHTT
VPQAWAALATALEQDDALAEVFFSRLTSMAYGGASMGQDIYERIQEVAVRVTGERISLSAGYGATETSPTASNVHWPNDK
MGLIGLPLPGNTFKLVPAGDKLELRVKGVNVTPGYFRNAEKTAEAFDEEGYYKLGDAVKFVDPAEPERGLAFDGRTVEEF
KLASGTWVSAGAVRVAAVAACGGALSDAVVCGLNKDEIGLLGFLNLAWCQRLVGEALPADQLVTHPKVLEAVKAGLSAHN
KAHPNASARITRILLQPVEPRADLGEITEKGYINQSRTQALRPDQLDLLFAAENAAGKILI

Specific function: Catalyzes the esterification, concomitant with transport, of exogenous long-chain fatty acids into metabolically active CoA thioesters for subsequent degradation or incorporation into phospholipids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Membrane; Peripheral membrane protein (Potential). Note=Partially membrane-associated (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI27477105, Length=567, Percent_Identity=23.9858906525573, Blast_Score=90, Evalue=7e-18,
Organism=Escherichia coli, GI1788107, Length=534, Percent_Identity=24.9063670411985, Blast_Score=88, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556552, Length=325, Percent_Identity=27.6923076923077, Blast_Score=87, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6324893, Length=288, Percent_Identity=25, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 67720; Mature: 67589

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTAHIPFREVPYLPLKLKIDRRPDGTIYLDSGHPLKAHPPHMLAPLVKWGAERPDQTW
CCCCCCCCHHHCCCCEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCH
LAERWPENPAADDWRRMTYADGLAAVRRIAAAFLSEGAGQDAPVMILSKNSIDNALVMYG
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHH
AMWAASPVVPVTPAYATLSQDLLRFRYIDQLTTPKFIYVEDGNEYQRALDSLDLTGRLVI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCEEEE
YSRNAPKVPRAVSLEDFAGQTGPAVDEAYARLTPKTVAKYMLTSGSTGEPKAVINTHGMV
EECCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEECCCEE
AANSKMIRSVWDEERLDAITGGPQVMVNFLPWSHTYGTHSILHNMLDWGGTMYICQGAPT
ECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCC
PARLPEMIRNLKEIPTTQHTTVPQAWAALATALEQDDALAEVFFSRLTSMAYGGASMGQD
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCCHH
IYERIQEVAVRVTGERISLSAGYGATETSPTASNVHWPNDKMGLIGLPLPGNTFKLVPAG
HHHHHHHHHHEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECC
DKLELRVKGVNVTPGYFRNAEKTAEAFDEEGYYKLGDAVKFVDPAEPERGLAFDGRTVEE
CEEEEEEEEEECCCHHHCCHHHHHHHHCCCCCEECCCEEEECCCCCCCCCCEECCCCHHH
FKLASGTWVSAGAVRVAAVAACGGALSDAVVCGLNKDEIGLLGFLNLAWCQRLVGEALPA
EECCCCCEEECCHHHHHHHHHHCCHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCH
DQLVTHPKVLEAVKAGLSAHNKAHPNASARITRILLQPVEPRADLGEITEKGYINQSRTQ
HHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHH
ALRPDQLDLLFAAENAAGKILI
CCCCCCEEEEEEECCCCCCEEC
>Mature Secondary Structure 
SKTAHIPFREVPYLPLKLKIDRRPDGTIYLDSGHPLKAHPPHMLAPLVKWGAERPDQTW
CCCCCCCHHHCCCCEEEEEECCCCCCEEEECCCCCCCCCCCHHHHHHHHHCCCCCCCCH
LAERWPENPAADDWRRMTYADGLAAVRRIAAAFLSEGAGQDAPVMILSKNSIDNALVMYG
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHH
AMWAASPVVPVTPAYATLSQDLLRFRYIDQLTTPKFIYVEDGNEYQRALDSLDLTGRLVI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCEEEE
YSRNAPKVPRAVSLEDFAGQTGPAVDEAYARLTPKTVAKYMLTSGSTGEPKAVINTHGMV
EECCCCCCCCCCCHHHHCCCCCCHHHHHHHHCCHHHHHHHHHCCCCCCCCCEEEECCCEE
AANSKMIRSVWDEERLDAITGGPQVMVNFLPWSHTYGTHSILHNMLDWGGTMYICQGAPT
ECCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCEEEEECCCCC
PARLPEMIRNLKEIPTTQHTTVPQAWAALATALEQDDALAEVFFSRLTSMAYGGASMGQD
HHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHCCHH
IYERIQEVAVRVTGERISLSAGYGATETSPTASNVHWPNDKMGLIGLPLPGNTFKLVPAG
HHHHHHHHHHEECCCEEEEECCCCCCCCCCCCCCCCCCCCCCCEEEECCCCCEEEEEECC
DKLELRVKGVNVTPGYFRNAEKTAEAFDEEGYYKLGDAVKFVDPAEPERGLAFDGRTVEE
CEEEEEEEEEECCCHHHCCHHHHHHHHCCCCCEECCCEEEECCCCCCCCCCEECCCCHHH
FKLASGTWVSAGAVRVAAVAACGGALSDAVVCGLNKDEIGLLGFLNLAWCQRLVGEALPA
EECCCCCEEECCHHHHHHHHHHCCHHHCCEEECCCCCCCHHHHHHHHHHHHHHHHHCCCH
DQLVTHPKVLEAVKAGLSAHNKAHPNASARITRILLQPVEPRADLGEITEKGYINQSRTQ
HHHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHCCCCCCHHHH
ALRPDQLDLLFAAENAAGKILI
CCCCCCEEEEEEECCCCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]