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The map label for this gene is lpdA2 [H]

Identifier: 114797713

GI number: 114797713

Start: 3426395

End: 3427798

Strand: Direct

Name: lpdA2 [H]

Synonym: HNE_3262

Alternate gene names: 114797713

Gene position: 3426395-3427798 (Clockwise)

Preceding gene: 114797190

Following gene: 114798792

Centisome position: 92.48

GC content: 62.61

Gene sequence:

>1404_bases
ATGGCAGAGACCTATGACGTCGTCATCATCGGCGGCGGCCCCGGCGGCTACAACTGCGCCATCCGCGCCGGCCAGCTTGG
CCTCAAGGTTGCCTGTATCGAGATGCGCGGCAAGCTCGGCGGCACCTGCCTGAATGTCGGCTGCATTCCGTCCAAGGCGT
TGCTGCACACCTCCCACCTTTTCAGCGCGGCGCAAAACGAGTTTGCCACGCTCGGCATCAAGACCGGCAAGGTAGAGCTC
GATCTGGCGCAGATGATGGGCCAGAAGGACGAGGCGGTTGAGGGGCTGACCAAGGGCGTCGAATTCCTGCTCAAGAAGAA
CAAGGCCGAATACATCAAGGGCAAGGGCAAGATCCTCGGCAAGGGCAAGGTCGAAGTGGCGCTGGCAGATGGCGGCACCC
GCGTGCTGGAAACGAAGAACATCGTCATCGCGACCGGCTCCGAGCCTGCCACCCTGCCCGGCATCGAGATTGATGAGGAG
CGGGTTGTTTCCAACACCGGCGCCCTCAGCCTGAAAGCTGTGCCGAAGAAACTCGTGCTGATCGGCGCGGGCGTGATTGG
GCTCGAGATGGGCTCTGTCTGGGCGCGGCTTGGCTCGGAAGTGACGGTCGTCGAGTATCTTGACCGCATCCTGCCGCCCG
CTGACGCGGAAGTGGCCAAGGAAGCCGAGCGCACCTTCAAGAAGCAGGGCCTCGCCTTCAAGCTGGGCACCAAGGTGACC
GGTATCGAGAAGACCAAGACAAAGCTGAAAGTGGCCGTGGAGCCTGCCAAGGGCGGAGCGGCGGAAACGCTGGACGCTGA
CATCGTGATCGTCGCCATCGGCCGAAAGCCGTATACGGAAGGCCTTGGGCTGGAAGCCGTGGGCGGGAAGACCGACAAGC
GCGGCGTGATCGAAGTGACCGATCATTTCAAGGTGGCAGACGGTGTCTGGGCGGTGGGCGACTGTATCCCCGGCCCGATG
CTGGCCCACAAAGCCGAAGACGATGGCACGGCGGTGGCCGAGCTGATCGCGGGCAAGGCGGGCCATGTGAACTATGACCT
CGTGCCGAGCGTCGTCTACACGAACCCGGAGATCGCTTGGGTCGGCAAGAATGAGGAAGAACTCAAGGCCGCTGGCGTTG
AGTATGTGAAGGGCAAGTTCCCCTTCATGGCAAACTCCCGTGCCCGGACGAACCATGAGACGGTGGGTTTCGTGAAAATC
CTCGCCGAAAAAGGCACCGACAAGATCCTTGGCGCGCACATGATCGGCGTGGGCGTTGGCGAGATGATCGCCGAAGTCTG
CGTGGCGATGGAGTTTGGCGCGTCTTCGGAAGACATCGCCCGCACCAGCCATGCCCACCCGACGCTTTCCGAAGCCGTTC
GCCAGGCGGCGATGGGCGTCGAAGGCTGGACGATGCAGATGTGA

Upstream 100 bases:

>100_bases
AAGCTAGGTCCAGTTTTCGGCCTTTGCAGATTGATTTTGAGGCAAGAATGCCTAGGTTGGCGCCAGATTCGCACCAGCCT
TCTCAAAGGAATATATTCCC

Downstream 100 bases:

>100_bases
ACCTGCCCCCTGCCCCGGCGGCATGTCAGATCATGACCGCCGGGGCAGCGCATGGCAGCTGTGCGCAGCTGTGTTTTACC
GCGCTTGGCGCTGGACGGGG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase 3; LPD-3 [H]

Number of amino acids: Translated: 467; Mature: 466

Protein sequence:

>467_residues
MAETYDVVIIGGGPGGYNCAIRAGQLGLKVACIEMRGKLGGTCLNVGCIPSKALLHTSHLFSAAQNEFATLGIKTGKVEL
DLAQMMGQKDEAVEGLTKGVEFLLKKNKAEYIKGKGKILGKGKVEVALADGGTRVLETKNIVIATGSEPATLPGIEIDEE
RVVSNTGALSLKAVPKKLVLIGAGVIGLEMGSVWARLGSEVTVVEYLDRILPPADAEVAKEAERTFKKQGLAFKLGTKVT
GIEKTKTKLKVAVEPAKGGAAETLDADIVIVAIGRKPYTEGLGLEAVGGKTDKRGVIEVTDHFKVADGVWAVGDCIPGPM
LAHKAEDDGTAVAELIAGKAGHVNYDLVPSVVYTNPEIAWVGKNEEELKAAGVEYVKGKFPFMANSRARTNHETVGFVKI
LAEKGTDKILGAHMIGVGVGEMIAEVCVAMEFGASSEDIARTSHAHPTLSEAVRQAAMGVEGWTMQM

Sequences:

>Translated_467_residues
MAETYDVVIIGGGPGGYNCAIRAGQLGLKVACIEMRGKLGGTCLNVGCIPSKALLHTSHLFSAAQNEFATLGIKTGKVEL
DLAQMMGQKDEAVEGLTKGVEFLLKKNKAEYIKGKGKILGKGKVEVALADGGTRVLETKNIVIATGSEPATLPGIEIDEE
RVVSNTGALSLKAVPKKLVLIGAGVIGLEMGSVWARLGSEVTVVEYLDRILPPADAEVAKEAERTFKKQGLAFKLGTKVT
GIEKTKTKLKVAVEPAKGGAAETLDADIVIVAIGRKPYTEGLGLEAVGGKTDKRGVIEVTDHFKVADGVWAVGDCIPGPM
LAHKAEDDGTAVAELIAGKAGHVNYDLVPSVVYTNPEIAWVGKNEEELKAAGVEYVKGKFPFMANSRARTNHETVGFVKI
LAEKGTDKILGAHMIGVGVGEMIAEVCVAMEFGASSEDIARTSHAHPTLSEAVRQAAMGVEGWTMQM
>Mature_466_residues
AETYDVVIIGGGPGGYNCAIRAGQLGLKVACIEMRGKLGGTCLNVGCIPSKALLHTSHLFSAAQNEFATLGIKTGKVELD
LAQMMGQKDEAVEGLTKGVEFLLKKNKAEYIKGKGKILGKGKVEVALADGGTRVLETKNIVIATGSEPATLPGIEIDEER
VVSNTGALSLKAVPKKLVLIGAGVIGLEMGSVWARLGSEVTVVEYLDRILPPADAEVAKEAERTFKKQGLAFKLGTKVTG
IEKTKTKLKVAVEPAKGGAAETLDADIVIVAIGRKPYTEGLGLEAVGGKTDKRGVIEVTDHFKVADGVWAVGDCIPGPML
AHKAEDDGTAVAELIAGKAGHVNYDLVPSVVYTNPEIAWVGKNEEELKAAGVEYVKGKFPFMANSRARTNHETVGFVKIL
AEKGTDKILGAHMIGVGVGEMIAEVCVAMEFGASSEDIARTSHAHPTLSEAVRQAAMGVEGWTMQM

Specific function: LPD-3 may substitute for lipoamide dehydrogenase of the 2-oxoglutarate dehydrogenase and pyruvate multienzyme complexes when the latter is inactive or missing [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=457, Percent_Identity=55.7986870897155, Blast_Score=509, Evalue=1e-144,
Organism=Homo sapiens, GI50301238, Length=463, Percent_Identity=29.5896328293737, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI33519430, Length=452, Percent_Identity=28.3185840707965, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI33519428, Length=452, Percent_Identity=28.3185840707965, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI33519426, Length=452, Percent_Identity=28.3185840707965, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI148277065, Length=452, Percent_Identity=28.3185840707965, Blast_Score=135, Evalue=7e-32,
Organism=Homo sapiens, GI148277071, Length=452, Percent_Identity=28.0973451327434, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI22035672, Length=458, Percent_Identity=29.0393013100437, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI291045266, Length=476, Percent_Identity=29.8319327731092, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI291045268, Length=470, Percent_Identity=28.0851063829787, Blast_Score=112, Evalue=6e-25,
Organism=Escherichia coli, GI1786307, Length=457, Percent_Identity=39.3873085339169, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI87082354, Length=462, Percent_Identity=30.952380952381, Blast_Score=205, Evalue=6e-54,
Organism=Escherichia coli, GI87081717, Length=456, Percent_Identity=29.3859649122807, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI1789915, Length=439, Percent_Identity=29.8405466970387, Blast_Score=159, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI32565766, Length=455, Percent_Identity=53.8461538461538, Blast_Score=486, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=28.3613445378151, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71983429, Length=447, Percent_Identity=31.3199105145414, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI71983419, Length=447, Percent_Identity=31.3199105145414, Blast_Score=138, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI71982272, Length=481, Percent_Identity=26.6112266112266, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17559934, Length=266, Percent_Identity=27.4436090225564, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6321091, Length=468, Percent_Identity=54.0598290598291, Blast_Score=486, Evalue=1e-138,
Organism=Saccharomyces cerevisiae, GI6325240, Length=471, Percent_Identity=32.9087048832272, Blast_Score=256, Evalue=7e-69,
Organism=Saccharomyces cerevisiae, GI6325166, Length=465, Percent_Identity=28.8172043010753, Blast_Score=154, Evalue=3e-38,
Organism=Drosophila melanogaster, GI21358499, Length=454, Percent_Identity=56.8281938325991, Blast_Score=511, Evalue=1e-145,
Organism=Drosophila melanogaster, GI24640553, Length=477, Percent_Identity=29.559748427673, Blast_Score=144, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24640549, Length=477, Percent_Identity=29.559748427673, Blast_Score=144, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24640551, Length=477, Percent_Identity=29.559748427673, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI17737741, Length=472, Percent_Identity=29.0254237288136, Blast_Score=135, Evalue=9e-32,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 49069; Mature: 48938

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAETYDVVIIGGGPGGYNCAIRAGQLGLKVACIEMRGKLGGTCLNVGCIPSKALLHTSHL
CCCEEEEEEEECCCCCCEEEEEECCCCEEEEEEEECCCCCCEEEEECCCCCHHHHHHHHH
FSAAQNEFATLGIKTGKVELDLAQMMGQKDEAVEGLTKGVEFLLKKNKAEYIKGKGKILG
HHHHCCCEEEEEEECCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHCCCHHEECCCCEEEE
KGKVEVALADGGTRVLETKNIVIATGSEPATLPGIEIDEERVVSNTGALSLKAVPKKLVL
CCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEECCCCEEEE
IGAGVIGLEMGSVWARLGSEVTVVEYLDRILPPADAEVAKEAERTFKKQGLAFKLGTKVT
EECCHHEEHHHHHHHHHCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCEEC
GIEKTKTKLKVAVEPAKGGAAETLDADIVIVAIGRKPYTEGLGLEAVGGKTDKRGVIEVT
CCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEE
DHFKVADGVWAVGDCIPGPMLAHKAEDDGTAVAELIAGKAGHVNYDLVPSVVYTNPEIAW
CCEEECCCCEEECCCCCCCHHEECCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCEEEE
VGKNEEELKAAGVEYVKGKFPFMANSRARTNHETVGFVKILAEKGTDKILGAHMIGVGVG
ECCCHHHHHHCCHHHHCCCCCEEECCCCCCCCCHHEEEEEEECCCCCHHHHHHHHHCCHH
EMIAEVCVAMEFGASSEDIARTSHAHPTLSEAVRQAAMGVEGWTMQM
HHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCEEECC
>Mature Secondary Structure 
AETYDVVIIGGGPGGYNCAIRAGQLGLKVACIEMRGKLGGTCLNVGCIPSKALLHTSHL
CCEEEEEEEECCCCCCEEEEEECCCCEEEEEEEECCCCCCEEEEECCCCCHHHHHHHHH
FSAAQNEFATLGIKTGKVELDLAQMMGQKDEAVEGLTKGVEFLLKKNKAEYIKGKGKILG
HHHHCCCEEEEEEECCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHCCCHHEECCCCEEEE
KGKVEVALADGGTRVLETKNIVIATGSEPATLPGIEIDEERVVSNTGALSLKAVPKKLVL
CCEEEEEEECCCEEEEEECCEEEEECCCCCCCCCCCCCHHHHHCCCCCEEEECCCCEEEE
IGAGVIGLEMGSVWARLGSEVTVVEYLDRILPPADAEVAKEAERTFKKQGLAFKLGTKVT
EECCHHEEHHHHHHHHHCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCCCEEEECCEEC
GIEKTKTKLKVAVEPAKGGAAETLDADIVIVAIGRKPYTEGLGLEAVGGKTDKRGVIEVT
CCCCCCEEEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCEEECCCCCCCCCEEEEE
DHFKVADGVWAVGDCIPGPMLAHKAEDDGTAVAELIAGKAGHVNYDLVPSVVYTNPEIAW
CCEEECCCCEEECCCCCCCHHEECCCCCCHHHHHHHCCCCCCCCCCCCCEEEEECCEEEE
VGKNEEELKAAGVEYVKGKFPFMANSRARTNHETVGFVKILAEKGTDKILGAHMIGVGVG
ECCCHHHHHHCCHHHHCCCCCEEECCCCCCCCCHHEEEEEEECCCCCHHHHHHHHHCCHH
EMIAEVCVAMEFGASSEDIARTSHAHPTLSEAVRQAAMGVEGWTMQM
HHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]