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The map label for this gene is gloA [H]

Identifier: 114797712

GI number: 114797712

Start: 3534053

End: 3534526

Strand: Direct

Name: gloA [H]

Synonym: HNE_3387

Alternate gene names: 114797712

Gene position: 3534053-3534526 (Clockwise)

Preceding gene: 114800513

Following gene: 114797092

Centisome position: 95.39

GC content: 65.4

Gene sequence:

>474_bases
ATGAGCCCTGAATTCCTCCACACCATGATCCGCGTCGGCGACATCGACGCTGCCAAGCGCTTCTTCTGCGACGGCCTCGG
CCTGGCCGAAGTCACCCGCTACGACAATGAAGCGGGCCGCTTCACCCTCGTCTTCCTCGCCGCCCCGGCAGACATTGAAC
GGGGTGGCGGCGTGCCCGAAGGCGCCTCGCCCACCTCGCTGAACATCCCGATGATCGAGCTCACCCATAACTGGGACGAG
AAGGATTATGAGGGCGGGCGCAATTTCGGCCACCTCGCCTATCAGGTCGAGGACATCTACGCCGCCACCCAGCGCTTCGC
CGATCTCGGCGTCCCCATTAGCCGCCCACCCCGCGACGGCCGCATGACCTTCATCCGCTCCCCGGACGGTATCTCCGTCG
AACTTCTGCAAAAGGGCAAACCCCAGGCCCCGCAGGAACCCTGGGCCAGCGCCCCGAATATCGGGGCGTGGTGA

Upstream 100 bases:

>100_bases
GCCTGGCCATCCTCATCCTCGCCATCGCGCTGCGGCGCTACTACACCCTGATGCCGCCCAATCTCCGGCAGGTCATCCGT
AATCTGAAGGACGCCCATTC

Downstream 100 bases:

>100_bases
CCCTTCCTCCCCCTCCCGCAGGCGGGAGAGGGCGGAACATGGCAAACGAAACGCTCACGGCCTGACGCCCCCACGAGCAA
GGCGAAGCGGCAGGCGCCCC

Product: putative lactoylglutathione lyase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 157; Mature: 156

Protein sequence:

>157_residues
MSPEFLHTMIRVGDIDAAKRFFCDGLGLAEVTRYDNEAGRFTLVFLAAPADIERGGGVPEGASPTSLNIPMIELTHNWDE
KDYEGGRNFGHLAYQVEDIYAATQRFADLGVPISRPPRDGRMTFIRSPDGISVELLQKGKPQAPQEPWASAPNIGAW

Sequences:

>Translated_157_residues
MSPEFLHTMIRVGDIDAAKRFFCDGLGLAEVTRYDNEAGRFTLVFLAAPADIERGGGVPEGASPTSLNIPMIELTHNWDE
KDYEGGRNFGHLAYQVEDIYAATQRFADLGVPISRPPRDGRMTFIRSPDGISVELLQKGKPQAPQEPWASAPNIGAW
>Mature_156_residues
SPEFLHTMIRVGDIDAAKRFFCDGLGLAEVTRYDNEAGRFTLVFLAAPADIERGGGVPEGASPTSLNIPMIELTHNWDEK
DYEGGRNFGHLAYQVEDIYAATQRFADLGVPISRPPRDGRMTFIRSPDGISVELLQKGKPQAPQEPWASAPNIGAW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=146, Percent_Identity=33.5616438356164, Blast_Score=78, Evalue=3e-15,
Organism=Escherichia coli, GI1787940, Length=139, Percent_Identity=28.0575539568345, Blast_Score=66, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6323639, Length=145, Percent_Identity=32.4137931034483, Blast_Score=67, Evalue=1e-12,
Organism=Drosophila melanogaster, GI20129743, Length=145, Percent_Identity=33.7931034482759, Blast_Score=78, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 17275; Mature: 17143

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSPEFLHTMIRVGDIDAAKRFFCDGLGLAEVTRYDNEAGRFTLVFLAAPADIERGGGVPE
CCHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCC
GASPTSLNIPMIELTHNWDEKDYEGGRNFGHLAYQVEDIYAATQRFADLGVPISRPPRDG
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCC
RMTFIRSPDGISVELLQKGKPQAPQEPWASAPNIGAW
CEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SPEFLHTMIRVGDIDAAKRFFCDGLGLAEVTRYDNEAGRFTLVFLAAPADIERGGGVPE
CHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHCCCCCCCEEEEEEECCCCCCCCCCCCC
GASPTSLNIPMIELTHNWDEKDYEGGRNFGHLAYQVEDIYAATQRFADLGVPISRPPRDG
CCCCCCCCCEEEEEECCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCCCCCCCCC
RMTFIRSPDGISVELLQKGKPQAPQEPWASAPNIGAW
CEEEEECCCCCEEEEECCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]