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The map label for this gene is pckA [H]

Identifier: 114797578

GI number: 114797578

Start: 2864742

End: 2866334

Strand: Direct

Name: pckA [H]

Synonym: HNE_2705

Alternate gene names: 114797578

Gene position: 2864742-2866334 (Clockwise)

Preceding gene: 114799724

Following gene: 114798142

Centisome position: 77.32

GC content: 61.46

Gene sequence:

>1593_bases
ATGAGCGATACCGTAGCAATCCTGGCCGAGCTGGGTGTGCAGACAAAATCCATTAAGAAAAACTGGAACGAGGCACGCCT
GTATGAAACGGCCGTTGCCAGCGGCGAAGCCCGCGTTGCCAAGGGCGGCGCGCTGGTGGTCGAAACCGGGCAACACACGG
GCCGCTCCGCCAAAGACAAGTTCACGGTGCGCGACGCGACGACCGAGAAAACCGTCTGGTGGGACAACAATGCCTCGATG
ACGCCCGAGCAGTTCGACGCTCTGTGGACGGACTTCAAAGCGCACCTCGCCAAGCAGGACATGTATTCGCAGGACCTCTT
CGGCGGCGCAGATCTCGACTATCGCCTGCCCGTCACTGTGGTGACCGAGTTTGCCTGGCACTCTCTCTTCATTCGCCACC
TTCTGCGCCTTCCGACAACGGATGAGCTCTCTGGCTTCAAGACCGAATTCACGATCATCAACTGCCCGAGCTTCCGCGCC
GATCCGGCCAAGCATGGCTGCCGCAGCGAGACCGTGATCGCGGTGAACTTCGCCAAGCGCCTCGTGCTCATCGGCGGCAC
CTCCTATGCTGGCGAAACGAAGAAGTCGGTCTTCACGATCCTCAACTACCTGCTGCCCAACCAGGGCGTGATGCCGATGC
ACTGCTCGGTCAACACATCTGACAAGGACGATGCGGCGATCTTCTTTGGCCTTTCAGGCACCGGCAAGACGACGCTTTCG
GCAGACGCTTCGCGCACACTGATCGGCGATGATGAGCATGGCTGGTCGGAGAACGGTCTCTTCAACTTTGAAGGCGGCTG
CTACGCCAAGATGATCAAGCTGTCGGCCGAGGCCGAGCCTGAAATCTTTGCCACCACGAAACAGTGGGGCACGGTGCTCG
AGAACGTCGTGATGGACGCGACAACGCGCGAACTCGATCTCGACTCGGCGGCGCTGGCGGAAAATTCGCGCGGCGCCTAT
CCGATCGAAGCGATTCCGAACGCGTCGCTGACGGGCCGTTGCGGCCAGCCGAAAAACCTCATCATGCTGACGGCTGACGC
CTATGGCATCATGCCGCCGATCGCCAAGCTGACGCCGGCGCAGGCGATGTATCACTTCCTCTCCGGCTATACCGCGCGTG
TCGCGGGCACCGAGAAAGGCGTGACCGAGCCTTCGGCCACCTTCTCCACCTGCTTTGGCGGCCCGTTCATGCCGCGTCAC
CCGTCGGAATATGGCAACCTGCTGCGCGAGCTGATCGCGCGTTACAATGTGGATTGCTGGCTGGTCTCCACCGGCTGGAC
CGGCGGGCCTTATGGCCAGGGCAACCGGATGCCGATCAAAGCAACGCGCGCGCTGCTGAACGCCGCGCTTGATGGCTCGC
TCAACACGGTCGAGTTCCGCAAGGATGAGACGTTCGGCTTCCTCGTGCCGGTTTCTGTGCCGGGCGTGGACGCCAAGATC
CTCGATCCGCGTTCGACCTGGGCTGACCCGGCCGCTTATGACAAGCAGGCGGCGAAACTGGCCGAAGAGTTCGTCGAGAA
CTTCAAGAAGTTCGAAGCCTATGTGGATGAGGCGGTGAAAGCCTCGGCGCCCAAGCCGAAGGTTACCGCGTAA

Upstream 100 bases:

>100_bases
GGGCACGGATGTTGCAAAAGCTTCTCACCAAGCGCTAATGACCCCTATGGTGGCAGGGCCGGGGCGCCCTAAGAGTTCGC
AAAAATTCGAGGACAGCGTA

Downstream 100 bases:

>100_bases
CCCCACACACTGCCTCTCGCAAAACAAGAGCCCGCCCGGTAAAACCTGGCGGGCTTTTTTTGATCTGAACTGCCCGTTTA
CGTCAACGGAAGGCTGGCCT

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 530; Mature: 529

Protein sequence:

>530_residues
MSDTVAILAELGVQTKSIKKNWNEARLYETAVASGEARVAKGGALVVETGQHTGRSAKDKFTVRDATTEKTVWWDNNASM
TPEQFDALWTDFKAHLAKQDMYSQDLFGGADLDYRLPVTVVTEFAWHSLFIRHLLRLPTTDELSGFKTEFTIINCPSFRA
DPAKHGCRSETVIAVNFAKRLVLIGGTSYAGETKKSVFTILNYLLPNQGVMPMHCSVNTSDKDDAAIFFGLSGTGKTTLS
ADASRTLIGDDEHGWSENGLFNFEGGCYAKMIKLSAEAEPEIFATTKQWGTVLENVVMDATTRELDLDSAALAENSRGAY
PIEAIPNASLTGRCGQPKNLIMLTADAYGIMPPIAKLTPAQAMYHFLSGYTARVAGTEKGVTEPSATFSTCFGGPFMPRH
PSEYGNLLRELIARYNVDCWLVSTGWTGGPYGQGNRMPIKATRALLNAALDGSLNTVEFRKDETFGFLVPVSVPGVDAKI
LDPRSTWADPAAYDKQAAKLAEEFVENFKKFEAYVDEAVKASAPKPKVTA

Sequences:

>Translated_530_residues
MSDTVAILAELGVQTKSIKKNWNEARLYETAVASGEARVAKGGALVVETGQHTGRSAKDKFTVRDATTEKTVWWDNNASM
TPEQFDALWTDFKAHLAKQDMYSQDLFGGADLDYRLPVTVVTEFAWHSLFIRHLLRLPTTDELSGFKTEFTIINCPSFRA
DPAKHGCRSETVIAVNFAKRLVLIGGTSYAGETKKSVFTILNYLLPNQGVMPMHCSVNTSDKDDAAIFFGLSGTGKTTLS
ADASRTLIGDDEHGWSENGLFNFEGGCYAKMIKLSAEAEPEIFATTKQWGTVLENVVMDATTRELDLDSAALAENSRGAY
PIEAIPNASLTGRCGQPKNLIMLTADAYGIMPPIAKLTPAQAMYHFLSGYTARVAGTEKGVTEPSATFSTCFGGPFMPRH
PSEYGNLLRELIARYNVDCWLVSTGWTGGPYGQGNRMPIKATRALLNAALDGSLNTVEFRKDETFGFLVPVSVPGVDAKI
LDPRSTWADPAAYDKQAAKLAEEFVENFKKFEAYVDEAVKASAPKPKVTA
>Mature_529_residues
SDTVAILAELGVQTKSIKKNWNEARLYETAVASGEARVAKGGALVVETGQHTGRSAKDKFTVRDATTEKTVWWDNNASMT
PEQFDALWTDFKAHLAKQDMYSQDLFGGADLDYRLPVTVVTEFAWHSLFIRHLLRLPTTDELSGFKTEFTIINCPSFRAD
PAKHGCRSETVIAVNFAKRLVLIGGTSYAGETKKSVFTILNYLLPNQGVMPMHCSVNTSDKDDAAIFFGLSGTGKTTLSA
DASRTLIGDDEHGWSENGLFNFEGGCYAKMIKLSAEAEPEIFATTKQWGTVLENVVMDATTRELDLDSAALAENSRGAYP
IEAIPNASLTGRCGQPKNLIMLTADAYGIMPPIAKLTPAQAMYHFLSGYTARVAGTEKGVTEPSATFSTCFGGPFMPRHP
SEYGNLLRELIARYNVDCWLVSTGWTGGPYGQGNRMPIKATRALLNAALDGSLNTVEFRKDETFGFLVPVSVPGVDAKIL
DPRSTWADPAAYDKQAAKLAEEFVENFKKFEAYVDEAVKASAPKPKVTA

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=500, Percent_Identity=45.2, Blast_Score=439, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6322950, Length=523, Percent_Identity=42.82982791587, Blast_Score=423, Evalue=1e-119,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 57806; Mature: 57675

Theoretical pI: Translated: 5.71; Mature: 5.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDTVAILAELGVQTKSIKKNWNEARLYETAVASGEARVAKGGALVVETGQHTGRSAKDK
CCCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCCCCCC
FTVRDATTEKTVWWDNNASMTPEQFDALWTDFKAHLAKQDMYSQDLFGGADLDYRLPVTV
EEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHH
VTEFAWHSLFIRHLLRLPTTDELSGFKTEFTIINCPSFRADPAKHGCRSETVIAVNFAKR
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHCCCCCCCEEEEEECCE
LVLIGGTSYAGETKKSVFTILNYLLPNQGVMPMHCSVNTSDKDDAAIFFGLSGTGKTTLS
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCEEEE
ADASRTLIGDDEHGWSENGLFNFEGGCYAKMIKLSAEAEPEIFATTKQWGTVLENVVMDA
CCCCCEEECCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHCC
TTRELDLDSAALAENSRGAYPIEAIPNASLTGRCGQPKNLIMLTADAYGIMPPIAKLTPA
CCCCCCCCHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHCCHH
QAMYHFLSGYTARVAGTEKGVTEPSATFSTCFGGPFMPRHPSEYGNLLRELIARYNVDCW
HHHHHHHCCCEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
LVSTGWTGGPYGQGNRMPIKATRALLNAALDGSLNTVEFRKDETFGFLVPVSVPGVDAKI
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCEEE
LDPRSTWADPAAYDKQAAKLAEEFVENFKKFEAYVDEAVKASAPKPKVTA
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SDTVAILAELGVQTKSIKKNWNEARLYETAVASGEARVAKGGALVVETGQHTGRSAKDK
CCHHHHHHHHCCCHHHHHCCCCHHHHHHHHHHCCCCEEECCCEEEEECCCCCCCCCCCC
FTVRDATTEKTVWWDNNASMTPEQFDALWTDFKAHLAKQDMYSQDLFGGADLDYRLPVTV
EEEECCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEECCHHH
VTEFAWHSLFIRHLLRLPTTDELSGFKTEFTIINCPSFRADPAKHGCRSETVIAVNFAKR
HHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECCCCCCCHHHCCCCCCCEEEEEECCE
LVLIGGTSYAGETKKSVFTILNYLLPNQGVMPMHCSVNTSDKDDAAIFFGLSGTGKTTLS
EEEEECCCCCCCHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCEEEEEECCCCCEEEE
ADASRTLIGDDEHGWSENGLFNFEGGCYAKMIKLSAEAEPEIFATTKQWGTVLENVVMDA
CCCCCEEECCCCCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEEHHHHHHHHHHHHHCC
TTRELDLDSAALAENSRGAYPIEAIPNASLTGRCGQPKNLIMLTADAYGIMPPIAKLTPA
CCCCCCCCHHHHHCCCCCCCCHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHCCHH
QAMYHFLSGYTARVAGTEKGVTEPSATFSTCFGGPFMPRHPSEYGNLLRELIARYNVDCW
HHHHHHHCCCEEEECCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCEE
LVSTGWTGGPYGQGNRMPIKATRALLNAALDGSLNTVEFRKDETFGFLVPVSVPGVDAKI
EEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEEECCCCCEEEEEEECCCCCEEE
LDPRSTWADPAAYDKQAAKLAEEFVENFKKFEAYVDEAVKASAPKPKVTA
ECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA