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The map label for this gene is pth

Identifier: 114797549

GI number: 114797549

Start: 1422836

End: 1423459

Strand: Reverse

Name: pth

Synonym: HNE_1396

Alternate gene names: 114797549

Gene position: 1423459-1422836 (Counterclockwise)

Preceding gene: 114797515

Following gene: 114797621

Centisome position: 38.42

GC content: 60.42

Gene sequence:

>624_bases
ATGCTGATCCTTTCCGGCCAGGGCAATCCAGGCGCCAAATATGCCAAGAACCGGCACAATGCCGGCTTTCTGGTGATCGA
CCGGATTCATGATGCCTATGGCTTCGGTCCCTGGCGCACGAAGTTTGATGCCGAGATTTCAGAAGGCACGGTTGAGACGG
CCAATGGCCGCCAGCGCGTGCTGCTGATCAAACCGCAGACCTTCTACAACGAGACGGGCCGGTCTGTTTCCAAGGCTGTC
ACCTTCTACAAGCTGCAACCCGAAGACGTGACCGTGTTTCATGACGAGATTGATCTGGCGCCGGGGCGGCTGCGCGTGAA
GCGCGGAGGCGGGCATTCGGGCAATAACGGCGCGCGTTCGATGATGGCGCATCTGGGCGAGAATTTCCGCCGCATACGGA
TTGGTGTGGGCCATCCTGGCGACAAGGCGATGGTGATGCCGCATGTGCTTTCGGATTTTCACAAAGTGGATCTGGAATGG
TTCGAACCGATGACTGAGGCCGTGTGCAAGGCCCTGCCCTTCCTGCTGGCGGGCGACGATGAGCGGTTCCAGACCGAGGT
GATGCGCCTGGCGCCTGCGCCCAAGCATGATCCCAAGCAGACGGCGCGCAAGGGCGAGGCTTAG

Upstream 100 bases:

>100_bases
GCCCCCAAGAAAGACTGATCCTTTCTGGACAATTCAGGGGCCGCCAGTCACAAGCTGGCGGCCTCTTTGATTCCGGGGCC
CCTGTCAGGAGCGAACCGTC

Downstream 100 bases:

>100_bases
GCCTTACCGGCCTTACGCCCGCCGTCCCTGAAATATTTCAGCGTGCCGCGGGTGATCACATCATCGCCCGGCGCAAGCTC
ATTGGCTTCATAGTCGGGCT

Product: peptidyl-tRNA hydrolase

Products: NA

Alternate protein names: PTH

Number of amino acids: Translated: 207; Mature: 207

Protein sequence:

>207_residues
MLILSGQGNPGAKYAKNRHNAGFLVIDRIHDAYGFGPWRTKFDAEISEGTVETANGRQRVLLIKPQTFYNETGRSVSKAV
TFYKLQPEDVTVFHDEIDLAPGRLRVKRGGGHSGNNGARSMMAHLGENFRRIRIGVGHPGDKAMVMPHVLSDFHKVDLEW
FEPMTEAVCKALPFLLAGDDERFQTEVMRLAPAPKHDPKQTARKGEA

Sequences:

>Translated_207_residues
MLILSGQGNPGAKYAKNRHNAGFLVIDRIHDAYGFGPWRTKFDAEISEGTVETANGRQRVLLIKPQTFYNETGRSVSKAV
TFYKLQPEDVTVFHDEIDLAPGRLRVKRGGGHSGNNGARSMMAHLGENFRRIRIGVGHPGDKAMVMPHVLSDFHKVDLEW
FEPMTEAVCKALPFLLAGDDERFQTEVMRLAPAPKHDPKQTARKGEA
>Mature_207_residues
MLILSGQGNPGAKYAKNRHNAGFLVIDRIHDAYGFGPWRTKFDAEISEGTVETANGRQRVLLIKPQTFYNETGRSVSKAV
TFYKLQPEDVTVFHDEIDLAPGRLRVKRGGGHSGNNGARSMMAHLGENFRRIRIGVGHPGDKAMVMPHVLSDFHKVDLEW
FEPMTEAVCKALPFLLAGDDERFQTEVMRLAPAPKHDPKQTARKGEA

Specific function: The natural substrate for this enzyme may be peptidyl- tRNAs which drop off the ribosome during protein synthesis

COG id: COG0193

COG function: function code J; Peptidyl-tRNA hydrolase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PTH family

Homologues:

Organism=Homo sapiens, GI50897284, Length=160, Percent_Identity=32.5, Blast_Score=89, Evalue=2e-18,
Organism=Escherichia coli, GI1787455, Length=150, Percent_Identity=40.6666666666667, Blast_Score=97, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PTH_HYPNA (Q0C2D0)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_760113.1
- ProteinModelPortal:   Q0C2D0
- SMR:   Q0C2D0
- STRING:   Q0C2D0
- GeneID:   4287384
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_1396
- NMPDR:   fig|228405.5.peg.1356
- TIGR:   HNE_1396
- eggNOG:   COG0193
- HOGENOM:   HBG610927
- OMA:   AMHRLHS
- PhylomeDB:   Q0C2D0
- ProtClustDB:   PRK05426
- BioCyc:   HNEP81032:HNE_1396-MONOMER
- GO:   GO:0005737
- GO:   GO:0006412
- HAMAP:   MF_00083
- InterPro:   IPR001328
- InterPro:   IPR018171
- Gene3D:   G3DSA:3.40.50.1470
- PANTHER:   PTHR17224
- TIGRFAMs:   TIGR00447

Pfam domain/function: PF01195 Pept_tRNA_hydro; SSF53178 Pept_tRNA_hydro

EC number: =3.1.1.29

Molecular weight: Translated: 23079; Mature: 23079

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: PS01195 PEPT_TRNA_HYDROL_1; PS01196 PEPT_TRNA_HYDROL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLILSGQGNPGAKYAKNRHNAGFLVIDRIHDAYGFGPWRTKFDAEISEGTVETANGRQRV
CEEECCCCCCCHHHHCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEE
LLIKPQTFYNETGRSVSKAVTFYKLQPEDVTVFHDEIDLAPGRLRVKRGGGHSGNNGARS
EEECCHHHHHHCCHHHHHHEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHH
MMAHLGENFRRIRIGVGHPGDKAMVMPHVLSDFHKVDLEWFEPMTEAVCKALPFLLAGDD
HHHHHCCCEEEEEEECCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCC
ERFQTEVMRLAPAPKHDPKQTARKGEA
HHHHHHHHHHCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MLILSGQGNPGAKYAKNRHNAGFLVIDRIHDAYGFGPWRTKFDAEISEGTVETANGRQRV
CEEECCCCCCCHHHHCCCCCCCEEEEEEHHHHHCCCCCCCCCCCCCCCCEEECCCCCEEE
LLIKPQTFYNETGRSVSKAVTFYKLQPEDVTVFHDEIDLAPGRLRVKRGGGHSGNNGARS
EEECCHHHHHHCCHHHHHHEEEEEECCCCEEEEECCCCCCCCEEEEEECCCCCCCHHHHH
MMAHLGENFRRIRIGVGHPGDKAMVMPHVLSDFHKVDLEWFEPMTEAVCKALPFLLAGDD
HHHHHCCCEEEEEEECCCCCCCCEEHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCC
ERFQTEVMRLAPAPKHDPKQTARKGEA
HHHHHHHHHHCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA