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The map label for this gene is limA [H]

Identifier: 114797389

GI number: 114797389

Start: 1578652

End: 1579029

Strand: Direct

Name: limA [H]

Synonym: HNE_1554

Alternate gene names: 114797389

Gene position: 1578652-1579029 (Clockwise)

Preceding gene: 114798744

Following gene: 114797863

Centisome position: 42.61

GC content: 54.5

Gene sequence:

>378_bases
ATGACTGAAAACGAAAAAATCATCCGGGAATTCATAGCCGCCTGGTCGCGTCTGGGCGTCGATGAAATTGTAAGCTATTT
CGCCGACGACGGCGTTTACCACAACATGCCCTTCAGGCCGGTGACAGGCCATGGTGCCCTGAAAGCATTTGTCGGCAATT
TCCTGAAGGACTGGACAGAGACGAACTGGGAGATCCTGACTCTGGTCAGCTCGGGCGATATTGTTGTAGCCGAGCGCCTC
GATCGCACGAGACTGGGAACGCAGGCTGTCGATCTTCCCTGTTGCGGCGTGTTTGTTATGGAAGGCGGGAAGATCAAAGT
CTGGCGGGACTATTTCGATATGGCGACTTACACGCGCGGTATATCCGGCGAGGCATGA

Upstream 100 bases:

>100_bases
ACTGATACGCCGTTTGCCTGGCGCACCGGCTGAACCGGCGGGCGCACCTGTTTCCTATATCCGCTTCTGAGACTTCAGGC
TCATCCGACAGGAGACATAG

Downstream 100 bases:

>100_bases
GAGACTGGCCGTGATTGATCCTTGTCAAGGCAGGCCGGGGAATTTTCATCCCGCTGTCAGATGCTGCGCACCTCGCGTTT
TGCCGCACCGAATCGGAGAG

Product: limonene-1,2-epoxide hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 125; Mature: 124

Protein sequence:

>125_residues
MTENEKIIREFIAAWSRLGVDEIVSYFADDGVYHNMPFRPVTGHGALKAFVGNFLKDWTETNWEILTLVSSGDIVVAERL
DRTRLGTQAVDLPCCGVFVMEGGKIKVWRDYFDMATYTRGISGEA

Sequences:

>Translated_125_residues
MTENEKIIREFIAAWSRLGVDEIVSYFADDGVYHNMPFRPVTGHGALKAFVGNFLKDWTETNWEILTLVSSGDIVVAERL
DRTRLGTQAVDLPCCGVFVMEGGKIKVWRDYFDMATYTRGISGEA
>Mature_124_residues
TENEKIIREFIAAWSRLGVDEIVSYFADDGVYHNMPFRPVTGHGALKAFVGNFLKDWTETNWEILTLVSSGDIVVAERLD
RTRLGTQAVDLPCCGVFVMEGGKIKVWRDYFDMATYTRGISGEA

Specific function: Catalyzes the conversion of limonene-1,2-epoxide to limonene-1,2-diol. Can use both the (-) and (+) isomers of limonene-1,2-epoxide as substrates and also has some activity with 1-methylcyclohexene oxide, cyclohexene oxide and indene oxide as substrates [

COG id: COG4308

COG function: function code Q; Limonene-1,2-epoxide hydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the limonene-1,2-epoxide hydrolase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013100 [H]

Pfam domain/function: PF07858 LEH [H]

EC number: =3.3.2.8 [H]

Molecular weight: Translated: 14085; Mature: 13954

Theoretical pI: Translated: 4.71; Mature: 4.71

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTENEKIIREFIAAWSRLGVDEIVSYFADDGVYHNMPFRPVTGHGALKAFVGNFLKDWTE
CCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCC
TNWEILTLVSSGDIVVAERLDRTRLGTQAVDLPCCGVFVMEGGKIKVWRDYFDMATYTRG
CCEEEEEEECCCCEEEECHHHHHHCCCCCCCCCCCEEEEEECCEEEEHHHHHHHHHHHCC
ISGEA
CCCCC
>Mature Secondary Structure 
TENEKIIREFIAAWSRLGVDEIVSYFADDGVYHNMPFRPVTGHGALKAFVGNFLKDWTE
CCHHHHHHHHHHHHHHCCHHHHHHHHHCCCEECCCCCCCCCCCHHHHHHHHHHHHHHCC
TNWEILTLVSSGDIVVAERLDRTRLGTQAVDLPCCGVFVMEGGKIKVWRDYFDMATYTRG
CCEEEEEEECCCCEEEECHHHHHHCCCCCCCCCCCEEEEEECCEEEEHHHHHHHHHHHCC
ISGEA
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9827564; 9748436 [H]