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The map label for this gene is rnhB

Identifier: 114797072

GI number: 114797072

Start: 2915483

End: 2916106

Strand: Direct

Name: rnhB

Synonym: HNE_2755

Alternate gene names: 114797072

Gene position: 2915483-2916106 (Clockwise)

Preceding gene: 114800515

Following gene: 114799084

Centisome position: 78.69

GC content: 66.03

Gene sequence:

>624_bases
ATGAGCGCGTTTCCCCTCATCATCGGCGTGGACGAAGCCGGGCGTGGCCCTTGGGCGGGGCCGGTGACGGCGGCGGCGGT
GATCCTGGACCCGGCCCAGCCTATCGAGGGGCTGACTGATTCCAAGAAGCTTTCGGAGAAGGCGCGCGACCGGCTGGCAC
CGCTGATCCGGGAGCGGGCGCTGGCCTGGTGCGTGGCCGAGGCGAGCGTGGAGGAGATCGACACGCTGAACATCCGGCAG
GCGACATTCCTGGCCATGCGGCGGGCAATTCTGGGACAATCCCGGACAGATCAGGACAGATCGGGACAGTTTGAGACAGT
TTCGGCCACCGCGGGCCTGATCCTGATTGATGGTAATGCCCTGCCCGGCGACCTGCCCGCGCCCGCCCGCGCGATCATCA
AGGGCGACCTCACCGAGCCCGCGATCTCAGCTGCCTCGATCCTGGCCAAGACGCACCGGGACGCGCAGATGGCCTCGCTC
TGTAATTTGTACCCTGGCTATGGATTCTCGGGACATAAGGGCTATGGCACAGCGGCGCATGCGGCGGCGCTTACCCGGCT
TGGGCCCTGCCCGGTTCACCGGCAGAGCTTTGCGCCGGTTCGCGCGCTTTTAAGACCTTGTTAA

Upstream 100 bases:

>100_bases
TGCTGACCGAGGCGGGGGAAGATGACCAGCCCTTCCTCCGCGCGAGGCCAATGCAGCCCCGATATTGGCAGGCAGTGCCG
ACTCCTGTTGGGATAATGGG

Downstream 100 bases:

>100_bases
CCGGGACACCCGAATTTCGCCGCGGGATATTACGGGACGCGGCAGTATGTTTCTTCTGAACTGGTTTCTGTTTGGCTGGC
ACACCTGGTATGGCGAAAGC

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 207; Mature: 206

Protein sequence:

>207_residues
MSAFPLIIGVDEAGRGPWAGPVTAAAVILDPAQPIEGLTDSKKLSEKARDRLAPLIRERALAWCVAEASVEEIDTLNIRQ
ATFLAMRRAILGQSRTDQDRSGQFETVSATAGLILIDGNALPGDLPAPARAIIKGDLTEPAISAASILAKTHRDAQMASL
CNLYPGYGFSGHKGYGTAAHAAALTRLGPCPVHRQSFAPVRALLRPC

Sequences:

>Translated_207_residues
MSAFPLIIGVDEAGRGPWAGPVTAAAVILDPAQPIEGLTDSKKLSEKARDRLAPLIRERALAWCVAEASVEEIDTLNIRQ
ATFLAMRRAILGQSRTDQDRSGQFETVSATAGLILIDGNALPGDLPAPARAIIKGDLTEPAISAASILAKTHRDAQMASL
CNLYPGYGFSGHKGYGTAAHAAALTRLGPCPVHRQSFAPVRALLRPC
>Mature_206_residues
SAFPLIIGVDEAGRGPWAGPVTAAAVILDPAQPIEGLTDSKKLSEKARDRLAPLIRERALAWCVAEASVEEIDTLNIRQA
TFLAMRRAILGQSRTDQDRSGQFETVSATAGLILIDGNALPGDLPAPARAIIKGDLTEPAISAASILAKTHRDAQMASLC
NLYPGYGFSGHKGYGTAAHAAALTRLGPCPVHRQSFAPVRALLRPC

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=196, Percent_Identity=52.0408163265306, Blast_Score=181, Evalue=4e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_HYPNA (Q0BYK9)

Other databases:

- EMBL:   CP000158
- RefSeq:   YP_761434.1
- ProteinModelPortal:   Q0BYK9
- SMR:   Q0BYK9
- STRING:   Q0BYK9
- GeneID:   4287353
- GenomeReviews:   CP000158_GR
- KEGG:   hne:HNE_2755
- NMPDR:   fig|228405.5.peg.2641
- TIGR:   HNE_2755
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   IICGVDE
- PhylomeDB:   Q0BYK9
- ProtClustDB:   PRK00015
- BioCyc:   HNEP81032:HNE_2755-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 21844; Mature: 21713

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAFPLIIGVDEAGRGPWAGPVTAAAVILDPAQPIEGLTDSKKLSEKARDRLAPLIRERA
CCCCCEEEECCCCCCCCCCCHHHHHHHEECCCCHHCCCCCHHHHHHHHHHHHHHHHHHHH
LAWCVAEASVEEIDTLNIRQATFLAMRRAILGQSRTDQDRSGQFETVSATAGLILIDGNA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEECCC
LPGDLPAPARAIIKGDLTEPAISAASILAKTHRDAQMASLCNLYPGYGFSGHKGYGTAAH
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
AAALTRLGPCPVHRQSFAPVRALLRPC
HHHHHHCCCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SAFPLIIGVDEAGRGPWAGPVTAAAVILDPAQPIEGLTDSKKLSEKARDRLAPLIRERA
CCCCEEEECCCCCCCCCCCHHHHHHHEECCCCHHCCCCCHHHHHHHHHHHHHHHHHHHH
LAWCVAEASVEEIDTLNIRQATFLAMRRAILGQSRTDQDRSGQFETVSATAGLILIDGNA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEEEECCC
LPGDLPAPARAIIKGDLTEPAISAASILAKTHRDAQMASLCNLYPGYGFSGHKGYGTAAH
CCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHH
AAALTRLGPCPVHRQSFAPVRALLRPC
HHHHHHCCCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA