Click here to switch to the map view.

The map label for this gene is fliA [H]

Identifier: 114332399

GI number: 114332399

Start: 2564957

End: 2565667

Strand: Direct

Name: fliA [H]

Synonym: Neut_2447

Alternate gene names: 114332399

Gene position: 2564957-2565667 (Clockwise)

Preceding gene: 114332398

Following gene: 114332400

Centisome position: 96.39

GC content: 50.21

Gene sequence:

>711_bases
ATGTATACCGAAGCAGGAACCATTGATAAAGACCAGTTTGTTATTGAATTTACACCCCTGGTCAAACGGATTGCCTACCA
TATGATGGCCAGACTGCCAGCCAGCGTTCAGGTGGATGATCTGATTCAGGCAGGAATGATCGGACTATTGGATGCCATCA
ATCGTTACGAAGGTTCCTACGGACGTCAGTTTGAAAGCTACGCTGCCCAGCGTATCCGTGGTTCGATGCTGGATGAACTG
CGCGAAGCGGACTGGCTGCCTCGCAGCTTGCGTAGAAAAATGCGTCAGATCGAAACCGCTATGCGCACACTGGAACAACG
CCTTGGCTGCCCGCCCAGCGAACAGGAAATCGCGGATGAACTTAATCTGCCACTAGCCGAGTATCAGGAAATGCTGCAAG
AAGCGGGGGGCGGGCAACTGATCTATTACGAGGATTTTCAGGTAGATGAAGAAGATCATTTTCTTGATCATCTGTGTAAT
GACCAGGGTAATGAGCCACTAGACCAATTGCTGGAAAAAAATCTGAGAGAACTGCTGGTCGCCGCCATTGAAAAATTGCC
TGCCAAGGAAAAACTGGTCATGGGTATGTACTACGAACAGGAAATGAACCTGCGCGAAATTGGTGATGTGCTGGGCGTCA
GCGAATCACGCATCTGCCAGCTCCACACCCAGGCCATCTCGCGTTTAAGAACCCGCCTGCGGGATAAATAA

Upstream 100 bases:

>100_bases
CGGGAGCCTGGACAGATAACGACGGTGTGGAAAATTTCATGCGCAGACTGATACGAACCAGCCATCTTGATACGGCAGAT
TTTACGGTGTGAGTGAACCT

Downstream 100 bases:

>100_bases
CCTCTCCCTGAAAGCCGATCAGGCTGAAAATACAATAGATTACCTACAGTTTTCATGCAATTGGTATGATGTTGCCGCAC
GAATACGTGGAATGCCCAGC

Product: RNA polymerase, sigma 28 subunit

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MYTEAGTIDKDQFVIEFTPLVKRIAYHMMARLPASVQVDDLIQAGMIGLLDAINRYEGSYGRQFESYAAQRIRGSMLDEL
READWLPRSLRRKMRQIETAMRTLEQRLGCPPSEQEIADELNLPLAEYQEMLQEAGGGQLIYYEDFQVDEEDHFLDHLCN
DQGNEPLDQLLEKNLRELLVAAIEKLPAKEKLVMGMYYEQEMNLREIGDVLGVSESRICQLHTQAISRLRTRLRDK

Sequences:

>Translated_236_residues
MYTEAGTIDKDQFVIEFTPLVKRIAYHMMARLPASVQVDDLIQAGMIGLLDAINRYEGSYGRQFESYAAQRIRGSMLDEL
READWLPRSLRRKMRQIETAMRTLEQRLGCPPSEQEIADELNLPLAEYQEMLQEAGGGQLIYYEDFQVDEEDHFLDHLCN
DQGNEPLDQLLEKNLRELLVAAIEKLPAKEKLVMGMYYEQEMNLREIGDVLGVSESRICQLHTQAISRLRTRLRDK
>Mature_236_residues
MYTEAGTIDKDQFVIEFTPLVKRIAYHMMARLPASVQVDDLIQAGMIGLLDAINRYEGSYGRQFESYAAQRIRGSMLDEL
READWLPRSLRRKMRQIETAMRTLEQRLGCPPSEQEIADELNLPLAEYQEMLQEAGGGQLIYYEDFQVDEEDHFLDHLCN
DQGNEPLDQLLEKNLRELLVAAIEKLPAKEKLVMGMYYEQEMNLREIGDVLGVSESRICQLHTQAISRLRTRLRDK

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=233, Percent_Identity=48.068669527897, Blast_Score=224, Evalue=3e-60,
Organism=Escherichia coli, GI1789448, Length=206, Percent_Identity=29.6116504854369, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789098, Length=201, Percent_Identity=25.8706467661692, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27344; Mature: 27344

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
4.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
4.7 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYTEAGTIDKDQFVIEFTPLVKRIAYHMMARLPASVQVDDLIQAGMIGLLDAINRYEGSY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
GRQFESYAAQRIRGSMLDELREADWLPRSLRRKMRQIETAMRTLEQRLGCPPSEQEIADE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LNLPLAEYQEMLQEAGGGQLIYYEDFQVDEEDHFLDHLCNDQGNEPLDQLLEKNLRELLV
HCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AAIEKLPAKEKLVMGMYYEQEMNLREIGDVLGVSESRICQLHTQAISRLRTRLRDK
HHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MYTEAGTIDKDQFVIEFTPLVKRIAYHMMARLPASVQVDDLIQAGMIGLLDAINRYEGSY
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCHH
GRQFESYAAQRIRGSMLDELREADWLPRSLRRKMRQIETAMRTLEQRLGCPPSEQEIADE
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHH
LNLPLAEYQEMLQEAGGGQLIYYEDFQVDEEDHFLDHLCNDQGNEPLDQLLEKNLRELLV
HCCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AAIEKLPAKEKLVMGMYYEQEMNLREIGDVLGVSESRICQLHTQAISRLRTRLRDK
HHHHHCCCHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]