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The map label for this gene is prfC

Identifier: 114332394

GI number: 114332394

Start: 2557673

End: 2559280

Strand: Direct

Name: prfC

Synonym: Neut_2442

Alternate gene names: 114332394

Gene position: 2557673-2559280 (Clockwise)

Preceding gene: 114332393

Following gene: 114332395

Centisome position: 96.11

GC content: 53.48

Gene sequence:

>1608_bases
ATGACGATCGATCATGAGGTTAAACGTCGTCGGACGTTTGCCATTATTTCCCATCCGGACGCCGGTAAAACCACGTTGAC
GGAAAAATTACTGCTGTTTGCTGGCGCCATTCACATCGCCGGCAGTGTCAAAGCGCGTAAAGCCAGTCGCCACGCCACTT
CCGATTGGATGGAAATCGAAAAACAGCGCGGTATCTCTGTTGCCAGCTCTGTCATGCAGATGGAGTACCGGGGCTGTGTC
ATCAACTTGCTGGACACGCCCGGTCACCAGGATTTTTCCGAAGATACCTACCGCGTGCTGACCGCTGTCGATGCAGCACT
CATGGTCATCGATGCCGCCAACGGCGTGGAATCACAAACCTTGCGCCTGCTGCAGGTCTGCCGCGCCCGCAACACCCCGA
TCATTACTTTCGTCAACAAACTGGATCGAGAAGTACGCGAACCACTCGATCTGATTGATGAAATCGAGCGCACACTGGGA
ATGGACGTAGTTCCATTCACCTGGCCGGTCGGCTCGGGCAAACGCTTTCATGGCGTGTACGATTTGCGGCACGAACTGAT
GCGCGTCTTTCGGCCTGGTATGGATCATGCCGAGCAGAAAGATACAACCATCATTACCGATCTGGGCGATCCGGCAGTTA
CAAAACGCTTCGGAGCAAACCTGGAACAGGCTCGGCAGGAAATTGATCTGATCAAGGGTGCTGCACCGGAATTTGATCAG
GCAGCGTTTCTTGCCGGGCAGCAAACACCCGTATTTTTTGGCTCGGCAATCAACAATTTCGGCGTACAGGAAGTGCTGGA
TACACTGGTTGATCTGGCGCCACCACCCGGTACACGCAAAGCCATTCAGCGAGAAATCCTGCCGACTGAAAATAAATTTT
CCGGGGTGGTTTTCAAGATTCAGGCAAACATGAACCCTGCTCATCGGGATCGCATTGCCTTTGTGCGCATCTGCTCGGGT
GAATTCAGGCGTGGAATGAGTCTCAAGGTAGTCCGCAGCGGCAAGGATGTGCGCACCAGCACGGTAGTTTCCTTTCTTTC
ACAACGGCGTGAATTGCTGGAAACAGCTTATGCAGGTGACATTATCGGCATTCCCAATCATGGGACGCTACAACTGGCCG
ATACCCTGACCGAGGGAGACAACCTGCAATTTACCGGTTTGCCATTTTTTGCGCCGGAAATTTTCCAGACAGTCGAAATT
GCCGATCCATTACGCAGCAAGCAACTCAAGTTGGGATTGACTCAGCTGGGTGAAGAAGGCGCCATCCAGGTGTTCCGTCC
GCATCTCGGCAGTATCCTGCTGCTGGGTGCGGTGGGCCAGCTACAGTTTGAAGTCGTCACCCATCGTCTCAAGCATGAAT
ACGGTGTCGAGGCACGTGTCGCTCCTGCCAAATACCAACTGGCAAGGTGGGTAACTGCCGAAATGCCCCGGGAATTGCAG
CGTTTTATTGATGCCAATACGCATCGTATCGCCTACGATGCAGTGGATGCCCCCACTTTTCTGGCTTCTTTCTCTGCCGA
AATCAGCGTGGCTGAAGAAAACTGGCCCGGCATCCGCTTCCACAAAATGCGGGAACATGCTGGCCTGTTGTTTCAGACAG
CAAGCTGA

Upstream 100 bases:

>100_bases
TCTGCCGGGAAATTTATCCGGCGACCAGCCGGTTCAGCGCTACCCACACCACTTATTGCCATCTTTATCATTCAGTTTCT
CAAGAACCACAGGATTTTCA

Downstream 100 bases:

>100_bases
TTTCCCAGCAGAATCAACCCAATCCCTACTAAAAAGCCATTTAGGCAGCAGATGATTTAATTCACTCGCTGCCTTTCCCC
GGTTTGTTTTTCCTGTTACC

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3

Number of amino acids: Translated: 535; Mature: 534

Protein sequence:

>535_residues
MTIDHEVKRRRTFAIISHPDAGKTTLTEKLLLFAGAIHIAGSVKARKASRHATSDWMEIEKQRGISVASSVMQMEYRGCV
INLLDTPGHQDFSEDTYRVLTAVDAALMVIDAANGVESQTLRLLQVCRARNTPIITFVNKLDREVREPLDLIDEIERTLG
MDVVPFTWPVGSGKRFHGVYDLRHELMRVFRPGMDHAEQKDTTIITDLGDPAVTKRFGANLEQARQEIDLIKGAAPEFDQ
AAFLAGQQTPVFFGSAINNFGVQEVLDTLVDLAPPPGTRKAIQREILPTENKFSGVVFKIQANMNPAHRDRIAFVRICSG
EFRRGMSLKVVRSGKDVRTSTVVSFLSQRRELLETAYAGDIIGIPNHGTLQLADTLTEGDNLQFTGLPFFAPEIFQTVEI
ADPLRSKQLKLGLTQLGEEGAIQVFRPHLGSILLLGAVGQLQFEVVTHRLKHEYGVEARVAPAKYQLARWVTAEMPRELQ
RFIDANTHRIAYDAVDAPTFLASFSAEISVAEENWPGIRFHKMREHAGLLFQTAS

Sequences:

>Translated_535_residues
MTIDHEVKRRRTFAIISHPDAGKTTLTEKLLLFAGAIHIAGSVKARKASRHATSDWMEIEKQRGISVASSVMQMEYRGCV
INLLDTPGHQDFSEDTYRVLTAVDAALMVIDAANGVESQTLRLLQVCRARNTPIITFVNKLDREVREPLDLIDEIERTLG
MDVVPFTWPVGSGKRFHGVYDLRHELMRVFRPGMDHAEQKDTTIITDLGDPAVTKRFGANLEQARQEIDLIKGAAPEFDQ
AAFLAGQQTPVFFGSAINNFGVQEVLDTLVDLAPPPGTRKAIQREILPTENKFSGVVFKIQANMNPAHRDRIAFVRICSG
EFRRGMSLKVVRSGKDVRTSTVVSFLSQRRELLETAYAGDIIGIPNHGTLQLADTLTEGDNLQFTGLPFFAPEIFQTVEI
ADPLRSKQLKLGLTQLGEEGAIQVFRPHLGSILLLGAVGQLQFEVVTHRLKHEYGVEARVAPAKYQLARWVTAEMPRELQ
RFIDANTHRIAYDAVDAPTFLASFSAEISVAEENWPGIRFHKMREHAGLLFQTAS
>Mature_534_residues
TIDHEVKRRRTFAIISHPDAGKTTLTEKLLLFAGAIHIAGSVKARKASRHATSDWMEIEKQRGISVASSVMQMEYRGCVI
NLLDTPGHQDFSEDTYRVLTAVDAALMVIDAANGVESQTLRLLQVCRARNTPIITFVNKLDREVREPLDLIDEIERTLGM
DVVPFTWPVGSGKRFHGVYDLRHELMRVFRPGMDHAEQKDTTIITDLGDPAVTKRFGANLEQARQEIDLIKGAAPEFDQA
AFLAGQQTPVFFGSAINNFGVQEVLDTLVDLAPPPGTRKAIQREILPTENKFSGVVFKIQANMNPAHRDRIAFVRICSGE
FRRGMSLKVVRSGKDVRTSTVVSFLSQRRELLETAYAGDIIGIPNHGTLQLADTLTEGDNLQFTGLPFFAPEIFQTVEIA
DPLRSKQLKLGLTQLGEEGAIQVFRPHLGSILLLGAVGQLQFEVVTHRLKHEYGVEARVAPAKYQLARWVTAEMPRELQR
FIDANTHRIAYDAVDAPTFLASFSAEISVAEENWPGIRFHKMREHAGLLFQTAS

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily

Homologues:

Organism=Homo sapiens, GI18390331, Length=512, Percent_Identity=26.953125, Blast_Score=164, Evalue=2e-40,
Organism=Homo sapiens, GI19923640, Length=511, Percent_Identity=25.6360078277886, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI25306283, Length=380, Percent_Identity=26.5789473684211, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI25306287, Length=311, Percent_Identity=28.9389067524116, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI157426893, Length=159, Percent_Identity=31.4465408805031, Blast_Score=90, Evalue=6e-18,
Organism=Homo sapiens, GI94966754, Length=255, Percent_Identity=25.8823529411765, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=32.2147651006711, Blast_Score=77, Evalue=5e-14,
Organism=Homo sapiens, GI310132016, Length=115, Percent_Identity=35.6521739130435, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310110807, Length=115, Percent_Identity=35.6521739130435, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI310123363, Length=115, Percent_Identity=35.6521739130435, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790835, Length=523, Percent_Identity=51.8164435946463, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI1789738, Length=508, Percent_Identity=28.5433070866142, Blast_Score=165, Evalue=8e-42,
Organism=Escherichia coli, GI1788922, Length=187, Percent_Identity=32.620320855615, Blast_Score=101, Evalue=1e-22,
Organism=Escherichia coli, GI48994988, Length=184, Percent_Identity=30.9782608695652, Blast_Score=95, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17533571, Length=485, Percent_Identity=28.2474226804124, Blast_Score=170, Evalue=2e-42,
Organism=Caenorhabditis elegans, GI17556745, Length=178, Percent_Identity=37.0786516853933, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17557151, Length=132, Percent_Identity=34.8484848484849, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17506493, Length=165, Percent_Identity=32.1212121212121, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=33.8345864661654, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=33.8345864661654, Blast_Score=74, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=490, Percent_Identity=26.3265306122449, Blast_Score=163, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6322359, Length=517, Percent_Identity=24.1779497098646, Blast_Score=119, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6323320, Length=151, Percent_Identity=27.8145695364238, Blast_Score=77, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=30.379746835443, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=30.379746835443, Blast_Score=76, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24582462, Length=486, Percent_Identity=27.1604938271605, Blast_Score=167, Evalue=2e-41,
Organism=Drosophila melanogaster, GI221458488, Length=521, Percent_Identity=26.1036468330134, Blast_Score=127, Evalue=2e-29,
Organism=Drosophila melanogaster, GI78706572, Length=174, Percent_Identity=32.7586206896552, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24585709, Length=157, Percent_Identity=34.3949044585987, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585711, Length=157, Percent_Identity=34.3949044585987, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24585713, Length=157, Percent_Identity=34.3949044585987, Blast_Score=82, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RF3_NITEC (Q0ADD1)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748616.1
- ProteinModelPortal:   Q0ADD1
- SMR:   Q0ADD1
- STRING:   Q0ADD1
- GeneID:   4273521
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2442
- NMPDR:   fig|335283.3.peg.1278
- eggNOG:   COG4108
- HOGENOM:   HBG285678
- OMA:   LQFEVVQ
- PhylomeDB:   Q0ADD1
- ProtClustDB:   PRK00741
- BioCyc:   NEUT335283:NEUT_2442-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00072
- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR00503
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF54980 EFG_III_V; SSF50447 Translat_factor

EC number: NA

Molecular weight: Translated: 59644; Mature: 59513

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIDHEVKRRRTFAIISHPDAGKTTLTEKLLLFAGAIHIAGSVKARKASRHATSDWMEIE
CCCCHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHH
KQRGISVASSVMQMEYRGCVINLLDTPGHQDFSEDTYRVLTAVDAALMVIDAANGVESQT
HHCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHH
LRLLQVCRARNTPIITFVNKLDREVREPLDLIDEIERTLGMDVVPFTWPVGSGKRFHGVY
HHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHH
DLRHELMRVFRPGMDHAEQKDTTIITDLGDPAVTKRFGANLEQARQEIDLIKGAAPEFDQ
HHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
AAFLAGQQTPVFFGSAINNFGVQEVLDTLVDLAPPPGTRKAIQREILPTENKFSGVVFKI
HHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEEE
QANMNPAHRDRIAFVRICSGEFRRGMSLKVVRSGKDVRTSTVVSFLSQRRELLETAYAGD
EECCCCCHHCCEEEEEEECHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
IIGIPNHGTLQLADTLTEGDNLQFTGLPFFAPEIFQTVEIADPLRSKQLKLGLTQLGEEG
EEECCCCCCEEEEEHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AIQVFRPHLGSILLLGAVGQLQFEVVTHRLKHEYGVEARVAPAKYQLARWVTAEMPRELQ
CEEEEHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHH
RFIDANTHRIAYDAVDAPTFLASFSAEISVAEENWPGIRFHKMREHAGLLFQTAS
HHHCCCCCEEEEECCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCEEEECC
>Mature Secondary Structure 
TIDHEVKRRRTFAIISHPDAGKTTLTEKLLLFAGAIHIAGSVKARKASRHATSDWMEIE
CCCHHHHHCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHH
KQRGISVASSVMQMEYRGCVINLLDTPGHQDFSEDTYRVLTAVDAALMVIDAANGVESQT
HHCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEECCCCCHHHH
LRLLQVCRARNTPIITFVNKLDREVREPLDLIDEIERTLGMDVVPFTWPVGSGKRFHGVY
HHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCCCHHH
DLRHELMRVFRPGMDHAEQKDTTIITDLGDPAVTKRFGANLEQARQEIDLIKGAAPEFDQ
HHHHHHHHHHCCCCCCCCCCCCEEEEECCCHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
AAFLAGQQTPVFFGSAINNFGVQEVLDTLVDLAPPPGTRKAIQREILPTENKFSGVVFKI
HHHHCCCCCCEEEHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCEEEEE
QANMNPAHRDRIAFVRICSGEFRRGMSLKVVRSGKDVRTSTVVSFLSQRRELLETAYAGD
EECCCCCHHCCEEEEEEECHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCC
IIGIPNHGTLQLADTLTEGDNLQFTGLPFFAPEIFQTVEIADPLRSKQLKLGLTQLGEEG
EEECCCCCCEEEEEHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
AIQVFRPHLGSILLLGAVGQLQFEVVTHRLKHEYGVEARVAPAKYQLARWVTAEMPRELQ
CEEEEHHHHHHEEEECCCCHHHHHHHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHH
RFIDANTHRIAYDAVDAPTFLASFSAEISVAEENWPGIRFHKMREHAGLLFQTAS
HHHCCCCCEEEEECCCCHHHHHHCCCEEEEECCCCCCCCHHHHHHHCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA