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The map label for this gene is sbcD [H]

Identifier: 114332376

GI number: 114332376

Start: 2533113

End: 2534357

Strand: Direct

Name: sbcD [H]

Synonym: Neut_2424

Alternate gene names: 114332376

Gene position: 2533113-2534357 (Clockwise)

Preceding gene: 114332375

Following gene: 114332377

Centisome position: 95.19

GC content: 55.82

Gene sequence:

>1245_bases
ATGAAAATCCTCCACACCTCTGACTGGCACCTTGGCCGCACGCTCTACGGCAGAAAACGCCACGAGGAGTTCGAGGCTTT
TCTGATCTGGCTGGCGGGGACGATTCAGCAGAATGAAATTGACGCTCTGCTGGTGGCGGGTGATGTCTTTGACACCAGCG
CTCCGAGCAATCGCACCCAGGAGCTTTATTATCGTTTCCTGTGCCAGGTGGCTGCCTCATCTTGTCGCCACGTTGTTGTC
ATCGCAGGCAATCACGACTCTCCTTCGTTCCTCAATGCCCCCAGGGAGCTGCTCAAGGCCCTTGATGTCCACGTGGTTGG
AAGTGCAACGATCGAGCACCCGGAAGACGAAGTGCTGGTGTTGCGTAATGAGCAGGGTGTGCCGGAACTGATTGTCTGTG
CCGTGCCCTATCTTCGCGACAGGGATATACGCGTGGCGGAAGCGGGTGAAAGTGTCGAGGACAAGGAGCGGAAATTGATT
GACGGCATCCGCACGCATTACGCCACTGTTGCTGCCCTGGCCGAACAGAAGCGCGAGGAACTTGGGGTTGATCTACCCAT
TGTGGGCATGGGACATCTGTTTACCGCTGGTGGACAAACTATTGACGGCGATGGCGTGCGTGAACTCTACGTTGGCTCCC
TGGCGCATGTGACGGCGGGGATTTTCCCTGCGAGTTTTGATTACCTGGCGCTTGGGCATCTCCACGTCCCGCAAAAGGTG
AACGGCCTTGAGACCATACGGTACAGCGGTTCTCCATTACCCATGGGGTTCGGAGAAGCCAAACAGCAGAAGAGCGTTTG
CCATGTTGCCTTTGGGCAGCACGATGGCCACAGCACAACTGCATCCGTACAATTGATCAACGTGCCGGTGTTTCAGAAAC
TCGAGCGTGTCAAGGGAGACTGGGATGGCATCTCAAGCCGTCTCCTCGAATTGTCGGCAACGGACAAAGCTTGGCTCGAA
ATCATTTACGACGGCATTGAGGTCATTGGCGACCTGCGCGAGCGCCTGGAGATAGCGATTGCCGGCACCCAAATGGAAAT
TCTGCGGATAAAGAACAACCGCATCATCGACCGCGTGCTGGGACAAATGCATGCAGAGGAAACACTCGACGATCTGAACG
TGAACGACGTGTTCGAGCGATGTCTCGCCGTTCATGACGTGCCTGAAGCGCAGCGGCCGAAATTGCACCGAGCCTATCAG
GAGACGCTCTTGTCTCTCTATGATGATAACGTACAGGCGGAGTAG

Upstream 100 bases:

>100_bases
TTATAGAGAATGAATCTATCTGCCAGCTTGATCCTGCGAAGTTTGACACAATCTCCGAATACGTTGTATTGGAATGTGAT
TGGATTCTGCAGGAGACTAT

Downstream 100 bases:

>100_bases
AGCGGCTATCGATGAGAATATTGCAGATACGCTTCAAAAATCTGAACTCGCTGGTCGGCGAATGGCAAATCGACCTGACG
CACCCGGCCTTTACCTCTGA

Product: nuclease SbcCD, D subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MKILHTSDWHLGRTLYGRKRHEEFEAFLIWLAGTIQQNEIDALLVAGDVFDTSAPSNRTQELYYRFLCQVAASSCRHVVV
IAGNHDSPSFLNAPRELLKALDVHVVGSATIEHPEDEVLVLRNEQGVPELIVCAVPYLRDRDIRVAEAGESVEDKERKLI
DGIRTHYATVAALAEQKREELGVDLPIVGMGHLFTAGGQTIDGDGVRELYVGSLAHVTAGIFPASFDYLALGHLHVPQKV
NGLETIRYSGSPLPMGFGEAKQQKSVCHVAFGQHDGHSTTASVQLINVPVFQKLERVKGDWDGISSRLLELSATDKAWLE
IIYDGIEVIGDLRERLEIAIAGTQMEILRIKNNRIIDRVLGQMHAEETLDDLNVNDVFERCLAVHDVPEAQRPKLHRAYQ
ETLLSLYDDNVQAE

Sequences:

>Translated_414_residues
MKILHTSDWHLGRTLYGRKRHEEFEAFLIWLAGTIQQNEIDALLVAGDVFDTSAPSNRTQELYYRFLCQVAASSCRHVVV
IAGNHDSPSFLNAPRELLKALDVHVVGSATIEHPEDEVLVLRNEQGVPELIVCAVPYLRDRDIRVAEAGESVEDKERKLI
DGIRTHYATVAALAEQKREELGVDLPIVGMGHLFTAGGQTIDGDGVRELYVGSLAHVTAGIFPASFDYLALGHLHVPQKV
NGLETIRYSGSPLPMGFGEAKQQKSVCHVAFGQHDGHSTTASVQLINVPVFQKLERVKGDWDGISSRLLELSATDKAWLE
IIYDGIEVIGDLRERLEIAIAGTQMEILRIKNNRIIDRVLGQMHAEETLDDLNVNDVFERCLAVHDVPEAQRPKLHRAYQ
ETLLSLYDDNVQAE
>Mature_414_residues
MKILHTSDWHLGRTLYGRKRHEEFEAFLIWLAGTIQQNEIDALLVAGDVFDTSAPSNRTQELYYRFLCQVAASSCRHVVV
IAGNHDSPSFLNAPRELLKALDVHVVGSATIEHPEDEVLVLRNEQGVPELIVCAVPYLRDRDIRVAEAGESVEDKERKLI
DGIRTHYATVAALAEQKREELGVDLPIVGMGHLFTAGGQTIDGDGVRELYVGSLAHVTAGIFPASFDYLALGHLHVPQKV
NGLETIRYSGSPLPMGFGEAKQQKSVCHVAFGQHDGHSTTASVQLINVPVFQKLERVKGDWDGISSRLLELSATDKAWLE
IIYDGIEVIGDLRERLEIAIAGTQMEILRIKNNRIIDRVLGQMHAEETLDDLNVNDVFERCLAVHDVPEAQRPKLHRAYQ
ETLLSLYDDNVQAE

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=410, Percent_Identity=37.3170731707317, Blast_Score=259, Evalue=2e-70,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 46101; Mature: 46101

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILHTSDWHLGRTLYGRKRHEEFEAFLIWLAGTIQQNEIDALLVAGDVFDTSAPSNRTQ
CCEEECCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHH
ELYYRFLCQVAASSCRHVVVIAGNHDSPSFLNAPRELLKALDVHVVGSATIEHPEDEVLV
HHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCEEEECCCCCCCCCCCEEE
LRNEQGVPELIVCAVPYLRDRDIRVAEAGESVEDKERKLIDGIRTHYATVAALAEQKREE
EECCCCCCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVDLPIVGMGHLFTAGGQTIDGDGVRELYVGSLAHVTAGIFPASFDYLALGHLHVPQKV
CCCCCCEECCCCEEECCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCEECCCCC
NGLETIRYSGSPLPMGFGEAKQQKSVCHVAFGQHDGHSTTASVQLINVPVFQKLERVKGD
CCCEEEEECCCCCCCCCCCHHHHCCEEEEECCCCCCCCCEEEEEEEECHHHHHHHHHCCC
WDGISSRLLELSATDKAWLEIIYDGIEVIGDLRERLEIAIAGTQMEILRIKNNRIIDRVL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEEECCCHHHHHHH
GQMHAEETLDDLNVNDVFERCLAVHDVPEAQRPKLHRAYQETLLSLYDDNVQAE
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKILHTSDWHLGRTLYGRKRHEEFEAFLIWLAGTIQQNEIDALLVAGDVFDTSAPSNRTQ
CCEEECCCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCCCHHH
ELYYRFLCQVAASSCRHVVVIAGNHDSPSFLNAPRELLKALDVHVVGSATIEHPEDEVLV
HHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHCEEEECCCCCCCCCCCEEE
LRNEQGVPELIVCAVPYLRDRDIRVAEAGESVEDKERKLIDGIRTHYATVAALAEQKREE
EECCCCCCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGVDLPIVGMGHLFTAGGQTIDGDGVRELYVGSLAHVTAGIFPASFDYLALGHLHVPQKV
CCCCCCEECCCCEEECCCCEECCCCHHHHHHHHHHHHHHCCCCCCCCCEEECCEECCCCC
NGLETIRYSGSPLPMGFGEAKQQKSVCHVAFGQHDGHSTTASVQLINVPVFQKLERVKGD
CCCEEEEECCCCCCCCCCCHHHHCCEEEEECCCCCCCCCEEEEEEEECHHHHHHHHHCCC
WDGISSRLLELSATDKAWLEIIYDGIEVIGDLRERLEIAIAGTQMEILRIKNNRIIDRVL
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEEECCCHHHHHHH
GQMHAEETLDDLNVNDVFERCLAVHDVPEAQRPKLHRAYQETLLSLYDDNVQAE
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]