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The map label for this gene is glcD [H]

Identifier: 114332345

GI number: 114332345

Start: 2501928

End: 2503298

Strand: Direct

Name: glcD [H]

Synonym: Neut_2388

Alternate gene names: 114332345

Gene position: 2501928-2503298 (Clockwise)

Preceding gene: 114332344

Following gene: 114332353

Centisome position: 94.02

GC content: 45.44

Gene sequence:

>1371_bases
ATGCTGCTGCCCGCTGAGTTTATTCAGTTACTACAGAATCTGATTCCATCCGACCGTCTCTATCTTGATCCGGTTGATTG
TTACGCTTACGCCTACGATAACAGCAGGAAGTATTTTCCGCCTGAGTGTGTCATTTTCCCTTTGACGACTGATGAAGTCG
TGAAGATAATCCAGTTATGTAATCGATTTAAGATTCCTTTAACACCTCGTGGGAGAGGTACCGGCACTGCTGGCGGCAGT
CTTGCCGAGCAAGGAGGCGCTTCACTTTCACTGGAACGCATGACACGGATCATATCGATCGATACGGCCAATCGAGTACT
CGTTGCAGAACCAGGTGTTTTAAATGAATCTGTCCAGGTGGCTGTCAAGCCTTCTGGTTTTTTTTGGCCACCCGATCCTT
CCAGTGCTGCTTTTTCAAGTATCGGCGGAAATCTTGCCACCTGTGCAGGGGGGCCACATGCTGTTAAATATGGCACAACC
CGCGATCATGTATTAGGGCTGACTGCAGTAACAGGACAGGGTGATATTATTAAAACGGGGTGCTATACCACCAAGGGTGT
TGTCGGGTACGACCTCACCCGGCTATTGATCGGATCAGAAGGTACACTTGCCGTTATAACCGAGGCAACACTTAAGTTAA
CCCCGTTACCTGCGGCAAGAAGCGGACTTGTTGCTCATTATCAGAATGCTGTGAGTTGTGCATCAGCGATAGCAACTATT
ATGAAAGAGCCGCAAGGACCCAGCGCGCTTGAATTTCTGGATTCCGGCTCACTTGATCTTATTCGTTCCAGGAATCCTGG
GATGTTGCCGGACGATTCTCGTGCCATGTTGATGATAGAAGTCGATGGTACAGAACACGAAATTTCGGAAACAACAACCA
GGCTTCTAGCTGCTTGCCAAAATTCCGGGTTGTTAAAGGCATTACCTGTCCAAAATGCTGCGGATTTGTGGAAGGCCAGA
AAAACGTTATCACCCTTGCTAAGAGATATCGCGCCGAAGAAAATAAATGAGGATATTGTAGTGCCTGTCGATAAAATTCC
CCGACTGTTAACCGGATTATCTTCATTATGTAAGCAATATCAGATACCCAACGTAAATTTTGGCCATGCTGGCAATGGAA
ATATTCATGTTAATCTGCTGATTGATCCGGATAATCCTGGAGAGAGTGGGCGTGCGTATAAATGCCTGAATCAGATATTT
GATCTGGTGATCAGCCTTAATGGAACACTTTCTGGTGAGCATGGTATCGGTTCTGAAAAGCGACCTTATATCAATAAAGA
ACTCAATGAGGCTACTTTGACTTTAATGAAGCAGATCAAATTAACATTTGATCCTTGTAATATCTTGAATCCAGGGAAAT
TATTTCCCTGA

Upstream 100 bases:

>100_bases
ACGTATTGCCAGTACGACGGAAAGTGTATTAAAGGTGGTTAAGGTATTTGAATATCTTGATTAGTAAGTACCTTTTCAGA
ACATCTTCAGGCATCAATAA

Downstream 100 bases:

>100_bases
ATGTTTTTCAGCCAGATAAACAAAAAAAACAGCCCGATAAAAATTACATCCTTATCGGGCTGTTTTTTTTAGAGAATTCG
TTTTTTTCTGGTATCAGAAA

Product: D-lactate dehydrogenase (cytochrome)

Products: pyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MLLPAEFIQLLQNLIPSDRLYLDPVDCYAYAYDNSRKYFPPECVIFPLTTDEVVKIIQLCNRFKIPLTPRGRGTGTAGGS
LAEQGGASLSLERMTRIISIDTANRVLVAEPGVLNESVQVAVKPSGFFWPPDPSSAAFSSIGGNLATCAGGPHAVKYGTT
RDHVLGLTAVTGQGDIIKTGCYTTKGVVGYDLTRLLIGSEGTLAVITEATLKLTPLPAARSGLVAHYQNAVSCASAIATI
MKEPQGPSALEFLDSGSLDLIRSRNPGMLPDDSRAMLMIEVDGTEHEISETTTRLLAACQNSGLLKALPVQNAADLWKAR
KTLSPLLRDIAPKKINEDIVVPVDKIPRLLTGLSSLCKQYQIPNVNFGHAGNGNIHVNLLIDPDNPGESGRAYKCLNQIF
DLVISLNGTLSGEHGIGSEKRPYINKELNEATLTLMKQIKLTFDPCNILNPGKLFP

Sequences:

>Translated_456_residues
MLLPAEFIQLLQNLIPSDRLYLDPVDCYAYAYDNSRKYFPPECVIFPLTTDEVVKIIQLCNRFKIPLTPRGRGTGTAGGS
LAEQGGASLSLERMTRIISIDTANRVLVAEPGVLNESVQVAVKPSGFFWPPDPSSAAFSSIGGNLATCAGGPHAVKYGTT
RDHVLGLTAVTGQGDIIKTGCYTTKGVVGYDLTRLLIGSEGTLAVITEATLKLTPLPAARSGLVAHYQNAVSCASAIATI
MKEPQGPSALEFLDSGSLDLIRSRNPGMLPDDSRAMLMIEVDGTEHEISETTTRLLAACQNSGLLKALPVQNAADLWKAR
KTLSPLLRDIAPKKINEDIVVPVDKIPRLLTGLSSLCKQYQIPNVNFGHAGNGNIHVNLLIDPDNPGESGRAYKCLNQIF
DLVISLNGTLSGEHGIGSEKRPYINKELNEATLTLMKQIKLTFDPCNILNPGKLFP
>Mature_456_residues
MLLPAEFIQLLQNLIPSDRLYLDPVDCYAYAYDNSRKYFPPECVIFPLTTDEVVKIIQLCNRFKIPLTPRGRGTGTAGGS
LAEQGGASLSLERMTRIISIDTANRVLVAEPGVLNESVQVAVKPSGFFWPPDPSSAAFSSIGGNLATCAGGPHAVKYGTT
RDHVLGLTAVTGQGDIIKTGCYTTKGVVGYDLTRLLIGSEGTLAVITEATLKLTPLPAARSGLVAHYQNAVSCASAIATI
MKEPQGPSALEFLDSGSLDLIRSRNPGMLPDDSRAMLMIEVDGTEHEISETTTRLLAACQNSGLLKALPVQNAADLWKAR
KTLSPLLRDIAPKKINEDIVVPVDKIPRLLTGLSSLCKQYQIPNVNFGHAGNGNIHVNLLIDPDNPGESGRAYKCLNQIF
DLVISLNGTLSGEHGIGSEKRPYINKELNEATLTLMKQIKLTFDPCNILNPGKLFP

Specific function: Unknown

COG id: COG0277

COG function: function code C; FAD/FMN-containing dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding PCMH-type domain [H]

Homologues:

Organism=Homo sapiens, GI37595756, Length=462, Percent_Identity=30.5194805194805, Blast_Score=226, Evalue=4e-59,
Organism=Homo sapiens, GI37595754, Length=485, Percent_Identity=29.0721649484536, Blast_Score=215, Evalue=8e-56,
Organism=Homo sapiens, GI119964728, Length=431, Percent_Identity=29.4663573085847, Blast_Score=169, Evalue=4e-42,
Organism=Homo sapiens, GI4501993, Length=233, Percent_Identity=31.7596566523605, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1789351, Length=436, Percent_Identity=33.4862385321101, Blast_Score=250, Evalue=1e-67,
Organism=Escherichia coli, GI1787977, Length=503, Percent_Identity=28.4294234592445, Blast_Score=147, Evalue=2e-36,
Organism=Escherichia coli, GI48994907, Length=498, Percent_Identity=24.4979919678715, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI48994914, Length=172, Percent_Identity=30.8139534883721, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71992373, Length=438, Percent_Identity=32.4200913242009, Blast_Score=217, Evalue=8e-57,
Organism=Caenorhabditis elegans, GI71992381, Length=438, Percent_Identity=30.8219178082192, Blast_Score=196, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI71992392, Length=382, Percent_Identity=31.413612565445, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17534361, Length=457, Percent_Identity=26.9146608315098, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17556096, Length=461, Percent_Identity=23.4273318872017, Blast_Score=107, Evalue=9e-24,
Organism=Saccharomyces cerevisiae, GI6320027, Length=448, Percent_Identity=27.6785714285714, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6320023, Length=451, Percent_Identity=27.9379157427938, Blast_Score=163, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6320764, Length=474, Percent_Identity=25.7383966244726, Blast_Score=145, Evalue=1e-35,
Organism=Drosophila melanogaster, GI18921117, Length=430, Percent_Identity=27.2093023255814, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24639277, Length=430, Percent_Identity=27.2093023255814, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24639275, Length=430, Percent_Identity=27.2093023255814, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI24653753, Length=199, Percent_Identity=31.1557788944724, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016166
- InterPro:   IPR016167
- InterPro:   IPR016164
- InterPro:   IPR016168
- InterPro:   IPR004113
- InterPro:   IPR004490
- InterPro:   IPR006094
- InterPro:   IPR016171 [H]

Pfam domain/function: PF02913 FAD-oxidase_C; PF01565 FAD_binding_4 [H]

EC number: 1.1.1.28

Molecular weight: Translated: 49032; Mature: 49032

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLPAEFIQLLQNLIPSDRLYLDPVDCYAYAYDNSRKYFPPECVIFPLTTDEVVKIIQLC
CCCHHHHHHHHHHHCCCCCEEECCHHEEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHH
NRFKIPLTPRGRGTGTAGGSLAEQGGASLSLERMTRIISIDTANRVLVAEPGVLNESVQV
CCCCCCCCCCCCCCCCCCCCHHHCCCCCEEHHHHHEEEEECCCCEEEEECCCCCCCCEEE
AVKPSGFFWPPDPSSAAFSSIGGNLATCAGGPHAVKYGTTRDHVLGLTAVTGQGDIIKTG
EECCCCCCCCCCCCHHHHHHCCCCEEECCCCCCEEECCCCCCCEEEEEEECCCCCEEEEC
CYTTKGVVGYDLTRLLIGSEGTLAVITEATLKLTPLPAARSGLVAHYQNAVSCASAIATI
CCCCCCCHHHHHEEEEECCCCCEEEEEECEEEEECCCCHHCCHHHHHHHHHHHHHHHHHH
MKEPQGPSALEFLDSGSLDLIRSRNPGMLPDDSRAMLMIEVDGTEHEISETTTRLLAACQ
HHCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHC
NSGLLKALPVQNAADLWKARKTLSPLLRDIAPKKINEDIVVPVDKIPRLLTGLSSLCKQY
CCCCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCEEECHHHHHHHHHHHHHHHHHH
QIPNVNFGHAGNGNIHVNLLIDPDNPGESGRAYKCLNQIFDLVISLNGTLSGEHGIGSEK
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCC
RPYINKELNEATLTLMKQIKLTFDPCNILNPGKLFP
CCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCC
>Mature Secondary Structure
MLLPAEFIQLLQNLIPSDRLYLDPVDCYAYAYDNSRKYFPPECVIFPLTTDEVVKIIQLC
CCCHHHHHHHHHHHCCCCCEEECCHHEEEEEEECCCCCCCCCEEEEECCHHHHHHHHHHH
NRFKIPLTPRGRGTGTAGGSLAEQGGASLSLERMTRIISIDTANRVLVAEPGVLNESVQV
CCCCCCCCCCCCCCCCCCCCHHHCCCCCEEHHHHHEEEEECCCCEEEEECCCCCCCCEEE
AVKPSGFFWPPDPSSAAFSSIGGNLATCAGGPHAVKYGTTRDHVLGLTAVTGQGDIIKTG
EECCCCCCCCCCCCHHHHHHCCCCEEECCCCCCEEECCCCCCCEEEEEEECCCCCEEEEC
CYTTKGVVGYDLTRLLIGSEGTLAVITEATLKLTPLPAARSGLVAHYQNAVSCASAIATI
CCCCCCCHHHHHEEEEECCCCCEEEEEECEEEEECCCCHHCCHHHHHHHHHHHHHHHHHH
MKEPQGPSALEFLDSGSLDLIRSRNPGMLPDDSRAMLMIEVDGTEHEISETTTRLLAACQ
HHCCCCCHHHHHHCCCCEEEEECCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHC
NSGLLKALPVQNAADLWKARKTLSPLLRDIAPKKINEDIVVPVDKIPRLLTGLSSLCKQY
CCCCEEECCCCCHHHHHHHHHHHHHHHHHCCHHHCCCCEEECHHHHHHHHHHHHHHHHHH
QIPNVNFGHAGNGNIHVNLLIDPDNPGESGRAYKCLNQIFDLVISLNGTLSGEHGIGSEK
CCCCCCCCCCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHEECCCCCCCCCCCCCCC
RPYINKELNEATLTLMKQIKLTFDPCNILNPGKLFP
CCCCCCHHHHHHHHHHHHHHEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: (R)-lactate; NAD+

Specific reaction: (R)-lactate + NAD+ = pyruvate + NADH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]