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The map label for this gene is dnaB [H]

Identifier: 114332335

GI number: 114332335

Start: 2494907

End: 2496304

Strand: Reverse

Name: dnaB [H]

Synonym: Neut_2378

Alternate gene names: 114332335

Gene position: 2496304-2494907 (Counterclockwise)

Preceding gene: 114332336

Following gene: 114332332

Centisome position: 93.81

GC content: 48.93

Gene sequence:

>1398_bases
ATGAATCCATCCACTACCAACTTTATTCAGAACCTTGCTGATAACAGTATTTACAAGCTCCCGCCCCACTCCATCGAGGC
TGAACAATCCGTCCTTGGTGGACTCATGCTGGATAATCAGGCATGGGACAAAGTAGCGGACATCATTATCGAGAGTGATT
TCTATCGCCAGGATCATCAACTCATTTACCAACACATCTGCCGGCTTATAGAACAAAGTAAGCCGGCAGATGTAATAACT
GTTGCAGAATCTCTTGAAAATGCGGCCCAACTGCAACACGCGGGTGGGCTAGCCTATATCGGTGCCATTGCACAGAATAC
TCCGTCTGCAGCCAACATCCGCCGTTATGCGGAAATCGTACGGGAACGTTCAATCATGCGCAAGCTGGCGCAAGTCAGCA
CACAAATAACTGATTCAGCTTACAATCCTGCAGGCCGTTCTGCGGGTGATCTGCTGGATGAAGCAGAAAGCAGAATATTT
GAAATCGCTGAACAAAGTGCGCACGGGAAACAAGGATTTGTTGATATCCAACCATTGCTCAAGCAAGTAGTTGAGCGAAT
CGAGATGCTCTATAACCGGAGCAATCCCAGCGATATTACCGGCATTCCATCCGGCTTCAGCGATCTCGATCAAAAAACTT
CTGGTTTCCAAGCCGGTGACCTGATCATCGTTGCCGGGCGACCTTCGATGGGAAAAACCGCCTTTGCGCTCAATATCGGA
GAACATGTCGCACTGGAAGCTGGCAAACCAGTGGCAGTGTTCAGTATGGAAATGGGGGGCGCACAGCTTGCCATGCGTAT
GTTGGGCTCAATCGGGCGATTGGATCAACACAAAGTACGCACAGGCCAACTGGATGATGATGACTGGCCCAGACTCACCC
ATGCATTGGGAAAGCTAAACGATGCGCCGATATTTATTGATGAAAGTGCTGCACTGAACTCTCTGGAGCTGCGTGCCCGC
GCTCGGCGTTTGTATCGTCAGCATGAAGGGCTGGGGCTCATCATTATTGACTACCTGCAACTCATGTCATCAACCTCGCC
CAACAGTGAAAGCCGCGCCGCCGAAATTTCAGAGATTTCACGCTCACTGAAGGCACTGGCAAAAGAATTGCAGGTTCCCG
TAATCGCTTTGTCGCAGCTGAATCGTGGCCTCGAACAACGCCCCAACAAACGTCCGATTATGTCTGATTTGCGTGAATCA
GGCGCTATCGAACAGGATGCAGATGTTATTTTGTTTATTTATCGGGATGAAGTTTATAATCCGGATACTCAGGACAAGGG
TATTGCTGAAATTATCATCGGCAAGCAGCGTAACGGGCCGATTGGCAAAGTTGATCTGACTTTTCTGGGCGAATTTACAA
GATTTGAAAATTGTGCACGTACCGCTGACTATTACTGA

Upstream 100 bases:

>100_bases
CTGGGTAAAGACCAAACAAAAAAAGGACTACCCGGTCCTTTTTTTGTTTCTCCATCAGACACTACCATCCGACCCTGGCT
ATTAGCCACCATTAATTCAC

Downstream 100 bases:

>100_bases
TTCAACCTGTCCGAACAATTAATTTATACCCATACGGCGGTACTGAATAGCTTCCGCCACATGCTTTCCCAAAATAGGTT
TACTGCCTGACAAATCTGCA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MNPSTTNFIQNLADNSIYKLPPHSIEAEQSVLGGLMLDNQAWDKVADIIIESDFYRQDHQLIYQHICRLIEQSKPADVIT
VAESLENAAQLQHAGGLAYIGAIAQNTPSAANIRRYAEIVRERSIMRKLAQVSTQITDSAYNPAGRSAGDLLDEAESRIF
EIAEQSAHGKQGFVDIQPLLKQVVERIEMLYNRSNPSDITGIPSGFSDLDQKTSGFQAGDLIIVAGRPSMGKTAFALNIG
EHVALEAGKPVAVFSMEMGGAQLAMRMLGSIGRLDQHKVRTGQLDDDDWPRLTHALGKLNDAPIFIDESAALNSLELRAR
ARRLYRQHEGLGLIIIDYLQLMSSTSPNSESRAAEISEISRSLKALAKELQVPVIALSQLNRGLEQRPNKRPIMSDLRES
GAIEQDADVILFIYRDEVYNPDTQDKGIAEIIIGKQRNGPIGKVDLTFLGEFTRFENCARTADYY

Sequences:

>Translated_465_residues
MNPSTTNFIQNLADNSIYKLPPHSIEAEQSVLGGLMLDNQAWDKVADIIIESDFYRQDHQLIYQHICRLIEQSKPADVIT
VAESLENAAQLQHAGGLAYIGAIAQNTPSAANIRRYAEIVRERSIMRKLAQVSTQITDSAYNPAGRSAGDLLDEAESRIF
EIAEQSAHGKQGFVDIQPLLKQVVERIEMLYNRSNPSDITGIPSGFSDLDQKTSGFQAGDLIIVAGRPSMGKTAFALNIG
EHVALEAGKPVAVFSMEMGGAQLAMRMLGSIGRLDQHKVRTGQLDDDDWPRLTHALGKLNDAPIFIDESAALNSLELRAR
ARRLYRQHEGLGLIIIDYLQLMSSTSPNSESRAAEISEISRSLKALAKELQVPVIALSQLNRGLEQRPNKRPIMSDLRES
GAIEQDADVILFIYRDEVYNPDTQDKGIAEIIIGKQRNGPIGKVDLTFLGEFTRFENCARTADYY
>Mature_465_residues
MNPSTTNFIQNLADNSIYKLPPHSIEAEQSVLGGLMLDNQAWDKVADIIIESDFYRQDHQLIYQHICRLIEQSKPADVIT
VAESLENAAQLQHAGGLAYIGAIAQNTPSAANIRRYAEIVRERSIMRKLAQVSTQITDSAYNPAGRSAGDLLDEAESRIF
EIAEQSAHGKQGFVDIQPLLKQVVERIEMLYNRSNPSDITGIPSGFSDLDQKTSGFQAGDLIIVAGRPSMGKTAFALNIG
EHVALEAGKPVAVFSMEMGGAQLAMRMLGSIGRLDQHKVRTGQLDDDDWPRLTHALGKLNDAPIFIDESAALNSLELRAR
ARRLYRQHEGLGLIIIDYLQLMSSTSPNSESRAAEISEISRSLKALAKELQVPVIALSQLNRGLEQRPNKRPIMSDLRES
GAIEQDADVILFIYRDEVYNPDTQDKGIAEIIIGKQRNGPIGKVDLTFLGEFTRFENCARTADYY

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=443, Percent_Identity=56.8848758465011, Blast_Score=508, Evalue=1e-145,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 51417; Mature: 51417

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPSTTNFIQNLADNSIYKLPPHSIEAEQSVLGGLMLDNQAWDKVADIIIESDFYRQDHQ
CCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LIYQHICRLIEQSKPADVITVAESLENAAQLQHAGGLAYIGAIAQNTPSAANIRRYAEIV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHH
RERSIMRKLAQVSTQITDSAYNPAGRSAGDLLDEAESRIFEIAEQSAHGKQGFVDIQPLL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHH
KQVVERIEMLYNRSNPSDITGIPSGFSDLDQKTSGFQAGDLIIVAGRPSMGKTAFALNIG
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECC
EHVALEAGKPVAVFSMEMGGAQLAMRMLGSIGRLDQHKVRTGQLDDDDWPRLTHALGKLN
CEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHHCCC
DAPIFIDESAALNSLELRARARRLYRQHEGLGLIIIDYLQLMSSTSPNSESRAAEISEIS
CCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
RSLKALAKELQVPVIALSQLNRGLEQRPNKRPIMSDLRESGAIEQDADVILFIYRDEVYN
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCC
PDTQDKGIAEIIIGKQRNGPIGKVDLTFLGEFTRFENCARTADYY
CCCCCCCHHHHEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNPSTTNFIQNLADNSIYKLPPHSIEAEQSVLGGLMLDNQAWDKVADIIIESDFYRQDHQ
CCCCHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
LIYQHICRLIEQSKPADVITVAESLENAAQLQHAGGLAYIGAIAQNTPSAANIRRYAEIV
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHH
RERSIMRKLAQVSTQITDSAYNPAGRSAGDLLDEAESRIFEIAEQSAHGKQGFVDIQPLL
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHH
KQVVERIEMLYNRSNPSDITGIPSGFSDLDQKTSGFQAGDLIIVAGRPSMGKTAFALNIG
HHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEECC
EHVALEAGKPVAVFSMEMGGAQLAMRMLGSIGRLDQHKVRTGQLDDDDWPRLTHALGKLN
CEEEECCCCCEEEEEECCCHHHHHHHHHHHHCCCCHHHCCCCCCCCCCHHHHHHHHHCCC
DAPIFIDESAALNSLELRARARRLYRQHEGLGLIIIDYLQLMSSTSPNSESRAAEISEIS
CCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHH
RSLKALAKELQVPVIALSQLNRGLEQRPNKRPIMSDLRESGAIEQDADVILFIYRDEVYN
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCC
PDTQDKGIAEIIIGKQRNGPIGKVDLTFLGEFTRFENCARTADYY
CCCCCCCHHHHEEECCCCCCCCEEEHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]