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The map label for this gene is pcm

Identifier: 114332283

GI number: 114332283

Start: 2448466

End: 2449134

Strand: Reverse

Name: pcm

Synonym: Neut_2323

Alternate gene names: 114332283

Gene position: 2449134-2448466 (Counterclockwise)

Preceding gene: 114332284

Following gene: 114332282

Centisome position: 92.04

GC content: 47.23

Gene sequence:

>669_bases
GTGAGTGTACGCCATTCTGGTATTGGCATGACTTCGCCGCGAACGAGGGTTCGCATGGTCGAGCGTTTAAGGGAGCAAGG
TGTTAGAGATGAGGTGGTATTGGCTGCAATGGGTTCTATTCCACGCCATCTTTTCGTTGAAGAAGCATTGGCATCCCGGG
CATATGAAGATGTGGCATTGCCAATTAATTACGGACAAACAATCTCCAGCCCCTGGATTGTAGGCCGTATGAGTGAATTG
CTGCGGAATGGTGGGAACAATTCGCTGAGAAAAGTACTGGAAATCGGAACAGGGTGCGGCTATCAGACTGCTGTTTTGGC
CAAGATAGCGAATGAAGTCTATTCAATTGAGCGGATAGGTCCCTTGTTGACACGTACTCGCATTCGATTGCGTGAATTGG
GTATTCGTAATATTTACCTGAAACATGCTGATGGAATGCGTGGATTGTTGGAGGCGGGTCCATTCGATGGAATAATGATG
ACTGCAGTTATTCCAGAGATACCAGAGACGCTCTTGGAACAATTGGCAATGGGTGGCAGGATGGTATTCCCGAAAGGAAA
CAGGCAGCAGTATCTTTGTGTAATTGATCATACTACCGAGGGTTTTGTTGAAACGATCTTGGATGAGGTAATGTTTGTGC
CCATTCTTCCCGGGACAATCAACAGGTAG

Upstream 100 bases:

>100_bases
CTCCCTTGATGTATTATCAGGCTGGTTCTTTTATGAAAGATAGTAGGAATAGTCAGATCCGGTTATTTGCTGGAAAGAAA
TAATAAAGGAGAGTTGGTGC

Downstream 100 bases:

>100_bases
GGAGTACTTATCTTCAAGGAATAGAGGCAGTCATGTTGTTGGCTGTATCTTAAACTTTCAATTTCTTATTTTCTATAGCA
ATTTAATCTAAATGTGGCGG

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT

Number of amino acids: Translated: 222; Mature: 221

Protein sequence:

>222_residues
MSVRHSGIGMTSPRTRVRMVERLREQGVRDEVVLAAMGSIPRHLFVEEALASRAYEDVALPINYGQTISSPWIVGRMSEL
LRNGGNNSLRKVLEIGTGCGYQTAVLAKIANEVYSIERIGPLLTRTRIRLRELGIRNIYLKHADGMRGLLEAGPFDGIMM
TAVIPEIPETLLEQLAMGGRMVFPKGNRQQYLCVIDHTTEGFVETILDEVMFVPILPGTINR

Sequences:

>Translated_222_residues
MSVRHSGIGMTSPRTRVRMVERLREQGVRDEVVLAAMGSIPRHLFVEEALASRAYEDVALPINYGQTISSPWIVGRMSEL
LRNGGNNSLRKVLEIGTGCGYQTAVLAKIANEVYSIERIGPLLTRTRIRLRELGIRNIYLKHADGMRGLLEAGPFDGIMM
TAVIPEIPETLLEQLAMGGRMVFPKGNRQQYLCVIDHTTEGFVETILDEVMFVPILPGTINR
>Mature_221_residues
SVRHSGIGMTSPRTRVRMVERLREQGVRDEVVLAAMGSIPRHLFVEEALASRAYEDVALPINYGQTISSPWIVGRMSELL
RNGGNNSLRKVLEIGTGCGYQTAVLAKIANEVYSIERIGPLLTRTRIRLRELGIRNIYLKHADGMRGLLEAGPFDGIMMT
AVIPEIPETLLEQLAMGGRMVFPKGNRQQYLCVIDHTTEGFVETILDEVMFVPILPGTINR

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789100, Length=211, Percent_Identity=43.1279620853081, Blast_Score=174, Evalue=4e-45,
Organism=Caenorhabditis elegans, GI71983477, Length=221, Percent_Identity=28.5067873303167, Blast_Score=74, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI193207222, Length=218, Percent_Identity=27.5229357798165, Blast_Score=65, Evalue=2e-11,
Organism=Drosophila melanogaster, GI17981723, Length=214, Percent_Identity=28.9719626168224, Blast_Score=74, Evalue=6e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PIMT_NITEC (Q0ADP2)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748505.1
- ProteinModelPortal:   Q0ADP2
- SMR:   Q0ADP2
- STRING:   Q0ADP2
- GeneID:   4272388
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2323
- NMPDR:   fig|335283.3.peg.88
- eggNOG:   COG2518
- HOGENOM:   HBG699907
- OMA:   YSVERIR
- PhylomeDB:   Q0ADP2
- ProtClustDB:   CLSK585560
- BioCyc:   NEUT335283:NEUT_2323-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00090
- InterPro:   IPR000682
- PANTHER:   PTHR11579
- TIGRFAMs:   TIGR00080

Pfam domain/function: PF01135 PCMT

EC number: =2.1.1.77

Molecular weight: Translated: 24732; Mature: 24601

Theoretical pI: Translated: 8.69; Mature: 8.69

Prosite motif: PS01279 PCMT

Important sites: ACT_SITE 69-69

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVRHSGIGMTSPRTRVRMVERLREQGVRDEVVLAAMGSIPRHLFVEEALASRAYEDVAL
CCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEE
PINYGQTISSPWIVGRMSELLRNGGNNSLRKVLEIGTGCGYQTAVLAKIANEVYSIERIG
ECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
PLLTRTRIRLRELGIRNIYLKHADGMRGLLEAGPFDGIMMTAVIPEIPETLLEQLAMGGR
HHHHHHHHHHHHCCHHHEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCE
MVFPKGNRQQYLCVIDHTTEGFVETILDEVMFVPILPGTINR
EECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SVRHSGIGMTSPRTRVRMVERLREQGVRDEVVLAAMGSIPRHLFVEEALASRAYEDVAL
CCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEE
PINYGQTISSPWIVGRMSELLRNGGNNSLRKVLEIGTGCGYQTAVLAKIANEVYSIERIG
ECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
PLLTRTRIRLRELGIRNIYLKHADGMRGLLEAGPFDGIMMTAVIPEIPETLLEQLAMGGR
HHHHHHHHHHHHCCHHHEEEECCCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCE
MVFPKGNRQQYLCVIDHTTEGFVETILDEVMFVPILPGTINR
EECCCCCCCEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA