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The map label for this gene is hprA [H]

Identifier: 114332203

GI number: 114332203

Start: 2368402

End: 2369370

Strand: Direct

Name: hprA [H]

Synonym: Neut_2243

Alternate gene names: 114332203

Gene position: 2368402-2369370 (Clockwise)

Preceding gene: 114332202

Following gene: 114332204

Centisome position: 89.0

GC content: 49.95

Gene sequence:

>969_bases
ATGCTTACGGTTTTCCTTGATTTCGGCTCCGTCACGCGCGGTGATATTGATCGGGCAGCGCTGGAGCAGGCCGTATCTCC
CTGGATTTATCATGACAACACCTCCCGTGAACAGGTTACAGAGCGGATCAAGGGAGCTGAAATCATCATCAGCAATAAAG
TGCAGCTGGATCGGACAGCCATATTTGCAGCAGATCAGCTAAAACTGATTTGTGTCGCAGCCACCGGTTATAACAACGTC
GATCTTGCTGCAGCCGCTGAGCGCAATATTCCGGTATGTAATGTACGCAACTATGCCACTAGTTCCGTTGCGCAACACAC
GCTTATGTTCATGCTGAACTTTGCCTGCCGTTTCGTGGAATATCGGCAGCTGATAAAAAAAGGGGGATGGCAGACCAGCA
CCTACTTCTGCCCGCTTGATTTCGGCGTTATGGAATTGGCAGGAAAGACGCTTGGTATCATCGGGCATGGCGAGCTCGGT
AGTGCTGTGGCAAATATAGCCAAAGCATTTGGTATGAAACTGTTGATCGCCGAATATAAATCCGCCTCATCCATCCGGCC
TGGGCGCATGGCATTCAATGATGTTATCCAACAAGCGGATTTCATTACGCTGCACTGCCCGTTGTCGGCAGATACCCGGC
ATTTGATTGGCAAGTGGGAATTTGATCTGATGAAACCCTCGGTATACCTTATTAATACAGCCAGAAGCGGCCTGATCAAT
GAAGACGACCTGCTGCAAGCCCTTTATTCTGGCCGTATTGCCGGCGCAGCTATCGACGTCCTGCAAGCAGAGCCGCCGGT
TAATGGCAATCCCTTGCTGGATTACCCTCATCCCAACCTGCTTATTACACCCCATTCCGCCTGGGCCAGTGTGGAATCTA
GACAACGCATGATCAACCTGCTGGCAGATAACATACGTAATTTCCTGCAAAACAAGCCTTTTAACCAGATTAATACAGCG
TTGCAGTAA

Upstream 100 bases:

>100_bases
ATCCAAGCTATCAAGAAAGACCGCTTTATTTGACAACGTACCTGGCTTTCAATACATTTCTTTGAAACATTCTTTTTAAA
TATCCAGCCATATAACAATT

Downstream 100 bases:

>100_bases
TTCTGGATACGCATATAGACCAGGTAATGTCCACCGCCGCACTCTACTGCTTAGAACCTGTTCAAGGAAAAATCTGAATG
AAAGCCACACCTCTTGCGAT

Product: glycerate dehydrogenase

Products: NA

Alternate protein names: GDH; Glyoxylate reductase; Hydroxypyruvate dehydrogenase; NADH-dependent hydroxypyruvate reductase; HPR; HPR-A [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MLTVFLDFGSVTRGDIDRAALEQAVSPWIYHDNTSREQVTERIKGAEIIISNKVQLDRTAIFAADQLKLICVAATGYNNV
DLAAAAERNIPVCNVRNYATSSVAQHTLMFMLNFACRFVEYRQLIKKGGWQTSTYFCPLDFGVMELAGKTLGIIGHGELG
SAVANIAKAFGMKLLIAEYKSASSIRPGRMAFNDVIQQADFITLHCPLSADTRHLIGKWEFDLMKPSVYLINTARSGLIN
EDDLLQALYSGRIAGAAIDVLQAEPPVNGNPLLDYPHPNLLITPHSAWASVESRQRMINLLADNIRNFLQNKPFNQINTA
LQ

Sequences:

>Translated_322_residues
MLTVFLDFGSVTRGDIDRAALEQAVSPWIYHDNTSREQVTERIKGAEIIISNKVQLDRTAIFAADQLKLICVAATGYNNV
DLAAAAERNIPVCNVRNYATSSVAQHTLMFMLNFACRFVEYRQLIKKGGWQTSTYFCPLDFGVMELAGKTLGIIGHGELG
SAVANIAKAFGMKLLIAEYKSASSIRPGRMAFNDVIQQADFITLHCPLSADTRHLIGKWEFDLMKPSVYLINTARSGLIN
EDDLLQALYSGRIAGAAIDVLQAEPPVNGNPLLDYPHPNLLITPHSAWASVESRQRMINLLADNIRNFLQNKPFNQINTA
LQ
>Mature_322_residues
MLTVFLDFGSVTRGDIDRAALEQAVSPWIYHDNTSREQVTERIKGAEIIISNKVQLDRTAIFAADQLKLICVAATGYNNV
DLAAAAERNIPVCNVRNYATSSVAQHTLMFMLNFACRFVEYRQLIKKGGWQTSTYFCPLDFGVMELAGKTLGIIGHGELG
SAVANIAKAFGMKLLIAEYKSASSIRPGRMAFNDVIQQADFITLHCPLSADTRHLIGKWEFDLMKPSVYLINTARSGLIN
EDDLLQALYSGRIAGAAIDVLQAEPPVNGNPLLDYPHPNLLITPHSAWASVESRQRMINLLADNIRNFLQNKPFNQINTA
LQ

Specific function: Plays a central role in assimilation of carbon. It converts hydroxypyruvate to glycerate as a key step in the serine cycle, and may also play an important role in C2 reactions, by interconverting glyoxylate and glycolate [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI145580578, Length=292, Percent_Identity=32.1917808219178, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI4557499, Length=292, Percent_Identity=32.1917808219178, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI61743967, Length=292, Percent_Identity=32.8767123287671, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI4557497, Length=292, Percent_Identity=32.8767123287671, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI145580575, Length=290, Percent_Identity=32.4137931034483, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI23308577, Length=297, Percent_Identity=30.6397306397306, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI6912396, Length=309, Percent_Identity=29.7734627831715, Blast_Score=118, Evalue=9e-27,
Organism=Escherichia coli, GI1789279, Length=266, Percent_Identity=31.9548872180451, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87082289, Length=252, Percent_Identity=32.5396825396825, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1787645, Length=257, Percent_Identity=29.1828793774319, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI87081824, Length=198, Percent_Identity=30.8080808080808, Blast_Score=76, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17532191, Length=252, Percent_Identity=34.1269841269841, Blast_Score=136, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI25147481, Length=303, Percent_Identity=28.0528052805281, Blast_Score=113, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6320925, Length=289, Percent_Identity=28.3737024221453, Blast_Score=124, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6324055, Length=325, Percent_Identity=28, Blast_Score=122, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6322116, Length=275, Percent_Identity=28.3636363636364, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6324964, Length=296, Percent_Identity=28.7162162162162, Blast_Score=104, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6324980, Length=187, Percent_Identity=28.3422459893048, Blast_Score=72, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24646446, Length=291, Percent_Identity=31.2714776632302, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24646448, Length=291, Percent_Identity=31.2714776632302, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24646452, Length=291, Percent_Identity=31.2714776632302, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24646450, Length=291, Percent_Identity=31.2714776632302, Blast_Score=134, Evalue=9e-32,
Organism=Drosophila melanogaster, GI62472511, Length=282, Percent_Identity=34.7517730496454, Blast_Score=133, Evalue=2e-31,
Organism=Drosophila melanogaster, GI28574286, Length=288, Percent_Identity=28.4722222222222, Blast_Score=122, Evalue=2e-28,
Organism=Drosophila melanogaster, GI19921140, Length=290, Percent_Identity=29.6551724137931, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI28571528, Length=312, Percent_Identity=29.4871794871795, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI24585516, Length=286, Percent_Identity=26.2237762237762, Blast_Score=114, Evalue=1e-25,
Organism=Drosophila melanogaster, GI24585514, Length=288, Percent_Identity=26.7361111111111, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI28574282, Length=288, Percent_Identity=26.7361111111111, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI45551003, Length=288, Percent_Identity=26.7361111111111, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28574284, Length=288, Percent_Identity=26.7361111111111, Blast_Score=106, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45552429, Length=288, Percent_Identity=26.7361111111111, Blast_Score=106, Evalue=2e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.29 [H]

Molecular weight: Translated: 35628; Mature: 35628

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00670 D_2_HYDROXYACID_DH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTVFLDFGSVTRGDIDRAALEQAVSPWIYHDNTSREQVTERIKGAEIIISNKVQLDRTA
CEEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCEEEEECCEEECHHE
IFAADQLKLICVAATGYNNVDLAAAAERNIPVCNVRNYATSSVAQHTLMFMLNFACRFVE
EEEECCEEEEEEEECCCCCCEEEEHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
YRQLIKKGGWQTSTYFCPLDFGVMELAGKTLGIIGHGELGSAVANIAKAFGMKLLIAEYK
HHHHHHHCCCCCCEEEEECCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHCHHHEEHHHC
SASSIRPGRMAFNDVIQQADFITLHCPLSADTRHLIGKWEFDLMKPSVYLINTARSGLIN
CCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHCCCEEEEEECCCEEEEECCCCCCCC
EDDLLQALYSGRIAGAAIDVLQAEPPVNGNPLLDYPHPNLLITPHSAWASVESRQRMINL
HHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCEEECCCCCEEEECCHHHHHHHHHHHHHHH
LADNIRNFLQNKPFNQINTALQ
HHHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure
MLTVFLDFGSVTRGDIDRAALEQAVSPWIYHDNTSREQVTERIKGAEIIISNKVQLDRTA
CEEEEEECCCCCCCCHHHHHHHHHCCCEEEECCCCHHHHHHHHCCCEEEEECCEEECHHE
IFAADQLKLICVAATGYNNVDLAAAAERNIPVCNVRNYATSSVAQHTLMFMLNFACRFVE
EEEECCEEEEEEEECCCCCCEEEEHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHH
YRQLIKKGGWQTSTYFCPLDFGVMELAGKTLGIIGHGELGSAVANIAKAFGMKLLIAEYK
HHHHHHHCCCCCCEEEEECCCHHHHHCCCEEEEEECCHHHHHHHHHHHHHCHHHEEHHHC
SASSIRPGRMAFNDVIQQADFITLHCPLSADTRHLIGKWEFDLMKPSVYLINTARSGLIN
CCCCCCCCHHHHHHHHHCCCEEEEECCCCCCCHHHCCCEEEEEECCCEEEEECCCCCCCC
EDDLLQALYSGRIAGAAIDVLQAEPPVNGNPLLDYPHPNLLITPHSAWASVESRQRMINL
HHHHHHHHHCCCCCHHHHHHHCCCCCCCCCCEEECCCCCEEEECCHHHHHHHHHHHHHHH
LADNIRNFLQNKPFNQINTALQ
HHHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8144463; 1729225; 1657886 [H]