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The map label for this gene is dapE

Identifier: 114332195

GI number: 114332195

Start: 2360030

End: 2361166

Strand: Reverse

Name: dapE

Synonym: Neut_2234

Alternate gene names: 114332195

Gene position: 2361166-2360030 (Counterclockwise)

Preceding gene: 114332196

Following gene: 114332192

Centisome position: 88.73

GC content: 51.72

Gene sequence:

>1137_bases
ATGCCCAATAGTACCCTGACACTTGCCCAGATGCTGATTGCACGTCGTTCCCTGACGCCAGATGATGACGGTTGCCAGAA
AATTCTTGTTCACCGGCTGGCCGGACTGGGATTCAACAGTGAAGCCATGAACTTTGGTGAGGTGGAGAATCTCTGGACGA
GAAAAGGTATTGACGGGCCGCTGGTATGTTTTGTCGGGCATACGGATGTTGTGCCGACTGGGCCGGTTGCGCAGTGGGAT
AGCGATCCTTTTACACCAACAGTCCGCGATGGCTTTCTCTATGGCCGTGGTGCAGCAGATATGAAGTCCTCTATCGCTGC
TTTTGTTACTGCGATTGAGGAATTTGTTGAGTTGCACCCGGATCACAAGGGTTCGATTGCGTTGCTGATTACCTCGGATG
AAGAAGGGCCGGCGGTGGAGGGGACAGTTAAAGTCGTGGAAACCCTTCAGGCACGCGGAGAGGTGATTGATTATTGTATC
GTGGGCGAACCTACCTGTACAGACCGGCTAGGGGATACTATTAAAAATGGGCGGCGCGGCAGTCTGTCAGGCAATCTGAC
GGTTAAAGGTATCCAGGGACACATTGCCTATCCTCATCTGGCCAGAAATCCAATTCACACTGCTGCACCTGCCATTGCGG
AGCTTGCCCAAACCGTATGGGATGATGGCAATGAATATTTCCCTGCCACCACCTGGCATATTTCCAATATCCGTGGCGGC
ACCGGCGCAACCAACGTTATTCCGGGAGAAGTCAATCTGCTGTTCAATTTCCGTTTTTCTACCGCCAGTACGGTTGAATC
ACTCAAGACGCGCGTGCATGAGATTCTGGATCGGCATGGACTGGAGTATGAACTGGTCTGGGAGCTTTCCGGCAAGCCTT
ACCTGACGCCCAAGGGAATACTGGCCGATGCACTCAGTGCAGCCATTCGCGAAGTAACAGGCGTTGAGCCGGAACTTTCC
ACCTCGGGCGGTACTTCCGATGGTCGTTTTATCGCTGATATCTGTTCGCAGGTGGTCGAATTCGGACCACGCAATGCAAC
TATTCATAAGATTAATGAATCCGTGGAAGTTGCGGATATAGAGCGACTATCTCGTATATACCGGTTGACATTGGAGAAAT
TGTTACTGCAGGATTGA

Upstream 100 bases:

>100_bases
TTTTGGAGGGGCACGCGGGCGTAATGCATTACGCATTTTTCAGCAACTCTGATTTTGCTGCCAAATTATTTCACTTAAAT
CTCAAACCAAGGAAACTCCC

Downstream 100 bases:

>100_bases
CGGTATTGCTACGATCCTATTGTCTATTGTGGCTGTTTTTGCGAACACCAACAGGTACGGAGCAATCCAGTAGCAGTACT
GGTAAAAAAGGCAGCCTGTT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MPNSTLTLAQMLIARRSLTPDDDGCQKILVHRLAGLGFNSEAMNFGEVENLWTRKGIDGPLVCFVGHTDVVPTGPVAQWD
SDPFTPTVRDGFLYGRGAADMKSSIAAFVTAIEEFVELHPDHKGSIALLITSDEEGPAVEGTVKVVETLQARGEVIDYCI
VGEPTCTDRLGDTIKNGRRGSLSGNLTVKGIQGHIAYPHLARNPIHTAAPAIAELAQTVWDDGNEYFPATTWHISNIRGG
TGATNVIPGEVNLLFNFRFSTASTVESLKTRVHEILDRHGLEYELVWELSGKPYLTPKGILADALSAAIREVTGVEPELS
TSGGTSDGRFIADICSQVVEFGPRNATIHKINESVEVADIERLSRIYRLTLEKLLLQD

Sequences:

>Translated_378_residues
MPNSTLTLAQMLIARRSLTPDDDGCQKILVHRLAGLGFNSEAMNFGEVENLWTRKGIDGPLVCFVGHTDVVPTGPVAQWD
SDPFTPTVRDGFLYGRGAADMKSSIAAFVTAIEEFVELHPDHKGSIALLITSDEEGPAVEGTVKVVETLQARGEVIDYCI
VGEPTCTDRLGDTIKNGRRGSLSGNLTVKGIQGHIAYPHLARNPIHTAAPAIAELAQTVWDDGNEYFPATTWHISNIRGG
TGATNVIPGEVNLLFNFRFSTASTVESLKTRVHEILDRHGLEYELVWELSGKPYLTPKGILADALSAAIREVTGVEPELS
TSGGTSDGRFIADICSQVVEFGPRNATIHKINESVEVADIERLSRIYRLTLEKLLLQD
>Mature_377_residues
PNSTLTLAQMLIARRSLTPDDDGCQKILVHRLAGLGFNSEAMNFGEVENLWTRKGIDGPLVCFVGHTDVVPTGPVAQWDS
DPFTPTVRDGFLYGRGAADMKSSIAAFVTAIEEFVELHPDHKGSIALLITSDEEGPAVEGTVKVVETLQARGEVIDYCIV
GEPTCTDRLGDTIKNGRRGSLSGNLTVKGIQGHIAYPHLARNPIHTAAPAIAELAQTVWDDGNEYFPATTWHISNIRGGT
GATNVIPGEVNLLFNFRFSTASTVESLKTRVHEILDRHGLEYELVWELSGKPYLTPKGILADALSAAIREVTGVEPELST
SGGTSDGRFIADICSQVVEFGPRNATIHKINESVEVADIERLSRIYRLTLEKLLLQD

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=54.9333333333333, Blast_Score=436, Evalue=1e-124,
Organism=Escherichia coli, GI1790395, Length=263, Percent_Identity=29.277566539924, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_NITEC (Q0ADY0)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748417.1
- ProteinModelPortal:   Q0ADY0
- SMR:   Q0ADY0
- STRING:   Q0ADY0
- MEROPS:   M20.010
- GeneID:   4274015
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2234
- NMPDR:   fig|335283.3.peg.1757
- eggNOG:   COG0624
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- PhylomeDB:   Q0ADY0
- ProtClustDB:   PRK13009
- BioCyc:   NEUT335283:NEUT_2234-MONOMER
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 41057; Mature: 40926

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 69-69 ACT_SITE 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNSTLTLAQMLIARRSLTPDDDGCQKILVHRLAGLGFNSEAMNFGEVENLWTRKGIDGP
CCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC
LVCFVGHTDVVPTGPVAQWDSDPFTPTVRDGFLYGRGAADMKSSIAAFVTAIEEFVELHP
EEEEECCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHCC
DHKGSIALLITSDEEGPAVEGTVKVVETLQARGEVIDYCIVGEPTCTDRLGDTIKNGRRG
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCC
SLSGNLTVKGIQGHIAYPHLARNPIHTAAPAIAELAQTVWDDGNEYFPATTWHISNIRGG
CCCCCEEEEECCCCEECCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
TGATNVIPGEVNLLFNFRFSTASTVESLKTRVHEILDRHGLEYELVWELSGKPYLTPKGI
CCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHH
LADALSAAIREVTGVEPELSTSGGTSDGRFIADICSQVVEFGPRNATIHKINESVEVADI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHH
ERLSRIYRLTLEKLLLQD
HHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PNSTLTLAQMLIARRSLTPDDDGCQKILVHRLAGLGFNSEAMNFGEVENLWTRKGIDGP
CCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCC
LVCFVGHTDVVPTGPVAQWDSDPFTPTVRDGFLYGRGAADMKSSIAAFVTAIEEFVELHP
EEEEECCCCCCCCCCCCCCCCCCCCCCHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHCC
DHKGSIALLITSDEEGPAVEGTVKVVETLQARGEVIDYCIVGEPTCTDRLGDTIKNGRRG
CCCCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCC
SLSGNLTVKGIQGHIAYPHLARNPIHTAAPAIAELAQTVWDDGNEYFPATTWHISNIRGG
CCCCCEEEEECCCCEECCHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCC
TGATNVIPGEVNLLFNFRFSTASTVESLKTRVHEILDRHGLEYELVWELSGKPYLTPKGI
CCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCHH
LADALSAAIREVTGVEPELSTSGGTSDGRFIADICSQVVEFGPRNATIHKINESVEVADI
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCEEEECCCCCHHHHH
ERLSRIYRLTLEKLLLQD
HHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA