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The map label for this gene is rluE [H]

Identifier: 114332166

GI number: 114332166

Start: 2331842

End: 2332333

Strand: Reverse

Name: rluE [H]

Synonym: Neut_2203

Alternate gene names: 114332166

Gene position: 2332333-2331842 (Counterclockwise)

Preceding gene: 114332167

Following gene: 114332165

Centisome position: 87.65

GC content: 56.3

Gene sequence:

>492_bases
TTGACCGATGATGGTTTCTTGCAGCATCGCATTGCGTCGCCAGTCCAGAAAATGCCGAAAACTTACTGGGTGCAGGTAGA
GGGTAATATTTCTGCAAGCGCTATAGCTGCGCTTTCAGCCGGTGTAATGCTTAAAGATAGGTGCACCCGGCCAGCTAAAG
CGTGCGCAATGGAAGAGCCGGCTCTGTGGCCCAGAGTTCCGCCGGTGCGCTGGCGCGATTCAATCCCTACCAGCTGGCTG
GAACTGATCATAACGGAAGGCCGTAACAGGCAGGTGCGACGAATGACGGCCGCCGTCGGCTTTCCAACGTTGCGACTGAT
CCGCTATCGTATTGGCAGCTGGTCGCTGGAAGGGTTGGAGCCGGGGGATTATCGTAGAATCCGCATCAATCTGCCCGCAG
CCTTGCCCTCAGGAAATCGACCTGCATTTGCCCGACCTGCAAAGCTACCAAAAAGGATCCGGAAATCACGACGCGGTAGC
CGTGGTCGTTGA

Upstream 100 bases:

>100_bases
GGCCGGACCAGAACGCCGATCTACCCTCGCAGACTGGATCGAAATTCCTAGCGTCTACCCGGCCGGGCGACTGGATTACG
ATTCCGAGGGCCTCCTGCTA

Downstream 100 bases:

>100_bases
AGACGCACACGGCCGATTCGGCGCGCTTATAGAAGAAGAGGGGATGACCTACCTGGTCAAGATGGAAAAAGGATGCAGCG
TTGGCACCTCTGCAATCGGC

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase E; rRNA-uridine isomerase E [H]

Number of amino acids: Translated: 163; Mature: 162

Protein sequence:

>163_residues
MTDDGFLQHRIASPVQKMPKTYWVQVEGNISASAIAALSAGVMLKDRCTRPAKACAMEEPALWPRVPPVRWRDSIPTSWL
ELIITEGRNRQVRRMTAAVGFPTLRLIRYRIGSWSLEGLEPGDYRRIRINLPAALPSGNRPAFARPAKLPKRIRKSRRGS
RGR

Sequences:

>Translated_163_residues
MTDDGFLQHRIASPVQKMPKTYWVQVEGNISASAIAALSAGVMLKDRCTRPAKACAMEEPALWPRVPPVRWRDSIPTSWL
ELIITEGRNRQVRRMTAAVGFPTLRLIRYRIGSWSLEGLEPGDYRRIRINLPAALPSGNRPAFARPAKLPKRIRKSRRGS
RGR
>Mature_162_residues
TDDGFLQHRIASPVQKMPKTYWVQVEGNISASAIAALSAGVMLKDRCTRPAKACAMEEPALWPRVPPVRWRDSIPTSWLE
LIITEGRNRQVRRMTAAVGFPTLRLIRYRIGSWSLEGLEPGDYRRIRINLPAALPSGNRPAFARPAKLPKRIRKSRRGSR
GR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2457 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase rsuA family [H]

Homologues:

Organism=Escherichia coli, GI87081838, Length=128, Percent_Identity=55.46875, Blast_Score=140, Evalue=5e-35,
Organism=Escherichia coli, GI1788510, Length=126, Percent_Identity=36.5079365079365, Blast_Score=71, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496 [H]

Pfam domain/function: PF00849 PseudoU_synth_2 [H]

EC number: NA

Molecular weight: Translated: 18441; Mature: 18310

Theoretical pI: Translated: 12.06; Mature: 12.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDDGFLQHRIASPVQKMPKTYWVQVEGNISASAIAALSAGVMLKDRCTRPAKACAMEEP
CCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCHHHHHCCCHHHHHCCCCC
ALWPRVPPVRWRDSIPTSWLELIITEGRNRQVRRMTAAVGFPTLRLIRYRIGSWSLEGLE
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCEEECCCC
PGDYRRIRINLPAALPSGNRPAFARPAKLPKRIRKSRRGSRGR
CCCEEEEEEECCEECCCCCCCCCCCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TDDGFLQHRIASPVQKMPKTYWVQVEGNISASAIAALSAGVMLKDRCTRPAKACAMEEP
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHCCCHHHHHCCCHHHHHCCCCC
ALWPRVPPVRWRDSIPTSWLELIITEGRNRQVRRMTAAVGFPTLRLIRYRIGSWSLEGLE
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHCCEEECCCC
PGDYRRIRINLPAALPSGNRPAFARPAKLPKRIRKSRRGSRGR
CCCEEEEEEECCEECCCCCCCCCCCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]