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The map label for this gene is rnhA

Identifier: 114332141

GI number: 114332141

Start: 2305996

End: 2306484

Strand: Reverse

Name: rnhA

Synonym: Neut_2176

Alternate gene names: 114332141

Gene position: 2306484-2305996 (Counterclockwise)

Preceding gene: 114332142

Following gene: 114332140

Centisome position: 86.68

GC content: 49.49

Gene sequence:

>489_bases
ATGCAACTGAAAAGTGATATGAAACGAGTGGAAATTTTTACGGATGGTGCCTGCAAGGGTAATCCGGGCCCGGGAGGATG
GGGGGTTTGTTTACATTTCAATGGCGAAACACGGGAATTTTTCGGGGGAGAGCCCGTAACCACAAACAATCGTATGGAGT
TACTGGCTGCCATCCGTGCCTTGCAGGAGCTGGAATCCCTGGAAGATAACGGACAGCAACACTTGCAGGTACAGCTGCAT
ACCGATTCGCAGTATGTGCAGAAAGGGATCAGTGAATGGATACACGGCTGGAAGAAGCGTGGGTGGCGGACAGCAGATAA
AAAACCAGTCAAGAATGAAGCGTTGTGGAGAGAGCTGGATGATTTATCTCAGCGTCATCAGGTTGAATGGTTCTGGGTGC
GGGGACATAACGGACATGCAGGTAATGAGCGGGCTGATAGGCTTGCAAATCAGGGAGTGGAATCGGTGTTATCTAAAAAA
GCAGATTGA

Upstream 100 bases:

>100_bases
CGGCAGTCGTTCCACAAATAGGGCTCGGGGGAGAGAGATAATCTCTGGAATTGCGGGCTGTTGCGAAAAGAGTGGATGGG
TTGATAACAGGAATAAGTGT

Downstream 100 bases:

>100_bases
TCGGACAGATTATGCGCTACGTCTTTCTTGATACTGAAACGACCGGCCTGGATCCGGCGCTGGGGCACCGTATTGTCGAA
ATAGCAGCGGTAGAAGTATG

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 162; Mature: 162

Protein sequence:

>162_residues
MQLKSDMKRVEIFTDGACKGNPGPGGWGVCLHFNGETREFFGGEPVTTNNRMELLAAIRALQELESLEDNGQQHLQVQLH
TDSQYVQKGISEWIHGWKKRGWRTADKKPVKNEALWRELDDLSQRHQVEWFWVRGHNGHAGNERADRLANQGVESVLSKK
AD

Sequences:

>Translated_162_residues
MQLKSDMKRVEIFTDGACKGNPGPGGWGVCLHFNGETREFFGGEPVTTNNRMELLAAIRALQELESLEDNGQQHLQVQLH
TDSQYVQKGISEWIHGWKKRGWRTADKKPVKNEALWRELDDLSQRHQVEWFWVRGHNGHAGNERADRLANQGVESVLSKK
AD
>Mature_162_residues
MQLKSDMKRVEIFTDGACKGNPGPGGWGVCLHFNGETREFFGGEPVTTNNRMELLAAIRALQELESLEDNGQQHLQVQLH
TDSQYVQKGISEWIHGWKKRGWRTADKKPVKNEALWRELDDLSQRHQVEWFWVRGHNGHAGNERADRLANQGVESVLSKK
AD

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=152, Percent_Identity=36.1842105263158, Blast_Score=86, Evalue=2e-17,
Organism=Escherichia coli, GI1786408, Length=143, Percent_Identity=58.041958041958, Blast_Score=174, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI115532542, Length=151, Percent_Identity=35.7615894039735, Blast_Score=97, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17505482, Length=137, Percent_Identity=34.3065693430657, Blast_Score=63, Evalue=5e-11,
Organism=Drosophila melanogaster, GI45552509, Length=151, Percent_Identity=40.3973509933775, Blast_Score=96, Evalue=8e-21,
Organism=Drosophila melanogaster, GI17137476, Length=151, Percent_Identity=40.3973509933775, Blast_Score=96, Evalue=9e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_NITEC (Q0AE34)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_748363.1
- ProteinModelPortal:   Q0AE34
- STRING:   Q0AE34
- GeneID:   4272773
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_2176
- NMPDR:   fig|335283.3.peg.227
- eggNOG:   COG0328
- HOGENOM:   HBG742437
- OMA:   SKVVIYT
- PhylomeDB:   Q0AE34
- ProtClustDB:   PRK00203
- BioCyc:   NEUT335283:NEUT_2176-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 18502; Mature: 18502

Theoretical pI: Translated: 7.22; Mature: 7.22

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLKSDMKRVEIFTDGACKGNPGPGGWGVCLHFNGETREFFGGEPVTTNNRMELLAAIRA
CCCHHHHHHHHEEECCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHH
LQELESLEDNGQQHLQVQLHTDSQYVQKGISEWIHGWKKRGWRTADKKPVKNEALWRELD
HHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
DLSQRHQVEWFWVRGHNGHAGNERADRLANQGVESVLSKKAD
HHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQLKSDMKRVEIFTDGACKGNPGPGGWGVCLHFNGETREFFGGEPVTTNNRMELLAAIRA
CCCHHHHHHHHEEECCCCCCCCCCCCCEEEEEECCCCHHHCCCCCCCCCHHHHHHHHHHH
LQELESLEDNGQQHLQVQLHTDSQYVQKGISEWIHGWKKRGWRTADKKPVKNEALWRELD
HHHHHHHHCCCCEEEEEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
DLSQRHQVEWFWVRGHNGHAGNERADRLANQGVESVLSKKAD
HHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA