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The map label for this gene is trmE [H]

Identifier: 114332120

GI number: 114332120

Start: 2280638

End: 2282017

Strand: Direct

Name: trmE [H]

Synonym: Neut_2155

Alternate gene names: 114332120

Gene position: 2280638-2282017 (Clockwise)

Preceding gene: 114332119

Following gene: 114332121

Centisome position: 85.7

GC content: 48.48

Gene sequence:

>1380_bases
ATGCAATGCCCTGCCACCTCCTTGGTGAAGAATAATGACACCATTGCAGCAATCGCCACACCACCTGGCCGTGGCGGCAT
TGGAATAGTCCGGATTTCAGGGACAAATCTAGAACAGCTTGCCCAGACAATCCTGGGCAAGCTTCCTGATCCACGTCACG
CCGGTTTATTCAACTTTCTTGATCAAAACAATCAAGTTATTGATCAGGGAATCGTGTTGTATTTCCCTTCCCCCAACTCC
TATACCGGAGAAGACGTACTGGAGCTGCACGGACATGGCGGCCCCGCTGTTATGAACCTGCTGTTGACCCGCTGCCTTCA
ACTGGGTGCTCGCCTAGCGGAACCGGGTGAATTTACACTGCGCGCTTTTCTCAACGAAAAACTTGATCTTGCACAAGCTG
AAGGAGTAGCCGACCTGATAGAAGCCAGCACTGCCAATGCCGCACGTTGTGCAGTGCGCTCATTACATGGGGAATTCTCA
TCGGCGATTCATCAGCTTGTAAGCGCGCTGATTGATTTGCGTGTGCTGGTCGAAGCAACGCTCGATTTCCCGGAAGAAGA
GATTGATTTTCTTCAGTCTGCCCATGCCGTGGAACAGCTGGCTTCAATCCAGACAAAACTGGAACAAGTATTGTCCGCAT
CCCGTCGGGGAAATTTATTACAGGAAGGGATCAAAGTCGTACTGACAGGCCAGCCCAATGTGGGCAAATCCAGCTTACTG
AATCGACTTGCTGGAGACGAGATAGCTATCGTCACCGAGATACCCGGTACAACAAGGGATACCATTCGGCAATCGATCGA
AATTGAAGGAATACCACTGCATTTGATTGACACCGCAGGATTGAGAGAAACCAGCGATATCGTTGAACAACATGGGATTG
CCCGTGCCTACGCTGCAATTGAACAGGCCGATCTGGTATTACTATTGGTTGACGGCCGCTATGGCGTGACCAAGGAAGAT
CACTCTGTACTGGCTCGTTTGCCGAAAGAACTGCCAGTATTGACTGTACACAACAAAATTGATTTATCCGGACAGCTCTC
TCGCATAGAAGAAGATACTTCAGGAACAGCTATCTATCTTTCAGTAAAAAGCGGTGAAGGCATAGAGCTGCTACGAACAG
TACTGCTTAAAACTGTTGGCTGGCAGACCAACATTGCTGGAGAAGGTGCTTACATGGCTCGCCAGCGGCATTTGCAAGCA
TTGCTGCATGCAAAAGAATTACTGAAAAGAGCAGAAACCTGGTTGCACACTGCAGATCAGTTAGAAATACTTGCTGAAGA
ACTTCGCCTTGCGCAACAAGCCCTGTCATCTATTACTGGCGAATTCACAAGCGATGATCTACTGGGTGAAATTTTTTCCA
ATTTCTGTATTGGAAAATAA

Upstream 100 bases:

>100_bases
AAAAAACCTGTTAACATTCCGAGAAGAATGCACAAAAGAACGAGAAAGAAATAAATATTTATAACTTCAACTGCTCTGTG
TCCCTTTTGATAAAGAAGGT

Downstream 100 bases:

>100_bases
ACTGGATAAAAAACTGCAATACATGCTTCTACGAAAATTCAATAAATTGTGACTTGTCAGTGGCAAACGAATATGGGTTC
AGGATATTCTTAATAGAGAT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 459

Protein sequence:

>459_residues
MQCPATSLVKNNDTIAAIATPPGRGGIGIVRISGTNLEQLAQTILGKLPDPRHAGLFNFLDQNNQVIDQGIVLYFPSPNS
YTGEDVLELHGHGGPAVMNLLLTRCLQLGARLAEPGEFTLRAFLNEKLDLAQAEGVADLIEASTANAARCAVRSLHGEFS
SAIHQLVSALIDLRVLVEATLDFPEEEIDFLQSAHAVEQLASIQTKLEQVLSASRRGNLLQEGIKVVLTGQPNVGKSSLL
NRLAGDEIAIVTEIPGTTRDTIRQSIEIEGIPLHLIDTAGLRETSDIVEQHGIARAYAAIEQADLVLLLVDGRYGVTKED
HSVLARLPKELPVLTVHNKIDLSGQLSRIEEDTSGTAIYLSVKSGEGIELLRTVLLKTVGWQTNIAGEGAYMARQRHLQA
LLHAKELLKRAETWLHTADQLEILAEELRLAQQALSSITGEFTSDDLLGEIFSNFCIGK

Sequences:

>Translated_459_residues
MQCPATSLVKNNDTIAAIATPPGRGGIGIVRISGTNLEQLAQTILGKLPDPRHAGLFNFLDQNNQVIDQGIVLYFPSPNS
YTGEDVLELHGHGGPAVMNLLLTRCLQLGARLAEPGEFTLRAFLNEKLDLAQAEGVADLIEASTANAARCAVRSLHGEFS
SAIHQLVSALIDLRVLVEATLDFPEEEIDFLQSAHAVEQLASIQTKLEQVLSASRRGNLLQEGIKVVLTGQPNVGKSSLL
NRLAGDEIAIVTEIPGTTRDTIRQSIEIEGIPLHLIDTAGLRETSDIVEQHGIARAYAAIEQADLVLLLVDGRYGVTKED
HSVLARLPKELPVLTVHNKIDLSGQLSRIEEDTSGTAIYLSVKSGEGIELLRTVLLKTVGWQTNIAGEGAYMARQRHLQA
LLHAKELLKRAETWLHTADQLEILAEELRLAQQALSSITGEFTSDDLLGEIFSNFCIGK
>Mature_459_residues
MQCPATSLVKNNDTIAAIATPPGRGGIGIVRISGTNLEQLAQTILGKLPDPRHAGLFNFLDQNNQVIDQGIVLYFPSPNS
YTGEDVLELHGHGGPAVMNLLLTRCLQLGARLAEPGEFTLRAFLNEKLDLAQAEGVADLIEASTANAARCAVRSLHGEFS
SAIHQLVSALIDLRVLVEATLDFPEEEIDFLQSAHAVEQLASIQTKLEQVLSASRRGNLLQEGIKVVLTGQPNVGKSSLL
NRLAGDEIAIVTEIPGTTRDTIRQSIEIEGIPLHLIDTAGLRETSDIVEQHGIARAYAAIEQADLVLLLVDGRYGVTKED
HSVLARLPKELPVLTVHNKIDLSGQLSRIEEDTSGTAIYLSVKSGEGIELLRTVLLKTVGWQTNIAGEGAYMARQRHLQA
LLHAKELLKRAETWLHTADQLEILAEELRLAQQALSSITGEFTSDDLLGEIFSNFCIGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=467, Percent_Identity=36.4025695931477, Blast_Score=233, Evalue=3e-61,
Organism=Homo sapiens, GI193082988, Length=497, Percent_Identity=34.4064386317907, Blast_Score=221, Evalue=8e-58,
Organism=Homo sapiens, GI193082993, Length=457, Percent_Identity=36.3238512035011, Blast_Score=219, Evalue=4e-57,
Organism=Escherichia coli, GI2367268, Length=456, Percent_Identity=55.921052631579, Blast_Score=494, Evalue=1e-141,
Organism=Escherichia coli, GI87082120, Length=105, Percent_Identity=43.8095238095238, Blast_Score=92, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17507259, Length=459, Percent_Identity=31.3725490196078, Blast_Score=192, Evalue=4e-49,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=33.265306122449, Blast_Score=220, Evalue=3e-58,
Organism=Drosophila melanogaster, GI45550813, Length=477, Percent_Identity=32.2851153039832, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR001806
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49727; Mature: 49727

Theoretical pI: Translated: 4.82; Mature: 4.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQCPATSLVKNNDTIAAIATPPGRGGIGIVRISGTNLEQLAQTILGKLPDPRHAGLFNFL
CCCCCCCHHCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHH
DQNNQVIDQGIVLYFPSPNSYTGEDVLELHGHGGPAVMNLLLTRCLQLGARLAEPGEFTL
CCCCCEECCCEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
RAFLNEKLDLAQAEGVADLIEASTANAARCAVRSLHGEFSSAIHQLVSALIDLRVLVEAT
HHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDFPEEEIDFLQSAHAVEQLASIQTKLEQVLSASRRGNLLQEGIKVVLTGQPNVGKSSLL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCHHHHH
NRLAGDEIAIVTEIPGTTRDTIRQSIEIEGIPLHLIDTAGLRETSDIVEQHGIARAYAAI
HHHCCCCEEEEEECCCCCHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHCHHHHHHHH
EQADLVLLLVDGRYGVTKEDHSVLARLPKELPVLTVHNKIDLSGQLSRIEEDTSGTAIYL
HHCCEEEEEECCCCCCCCHHHHHHHHCHHHCCEEEECCCCCCCHHHHHHHCCCCCCEEEE
SVKSGEGIELLRTVLLKTVGWQTNIAGEGAYMARQRHLQALLHAKELLKRAETWLHTADQ
EECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LEILAEELRLAQQALSSITGEFTSDDLLGEIFSNFCIGK
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MQCPATSLVKNNDTIAAIATPPGRGGIGIVRISGTNLEQLAQTILGKLPDPRHAGLFNFL
CCCCCCCHHCCCCCEEEEECCCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCHHHHH
DQNNQVIDQGIVLYFPSPNSYTGEDVLELHGHGGPAVMNLLLTRCLQLGARLAEPGEFTL
CCCCCEECCCEEEEECCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHH
RAFLNEKLDLAQAEGVADLIEASTANAARCAVRSLHGEFSSAIHQLVSALIDLRVLVEAT
HHHHHHHHHHHHHCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LDFPEEEIDFLQSAHAVEQLASIQTKLEQVLSASRRGNLLQEGIKVVLTGQPNVGKSSLL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCEEEEECCCCCCHHHHH
NRLAGDEIAIVTEIPGTTRDTIRQSIEIEGIPLHLIDTAGLRETSDIVEQHGIARAYAAI
HHHCCCCEEEEEECCCCCHHHHHHHHEECCCCEEEEECCCCCHHHHHHHHHCHHHHHHHH
EQADLVLLLVDGRYGVTKEDHSVLARLPKELPVLTVHNKIDLSGQLSRIEEDTSGTAIYL
HHCCEEEEEECCCCCCCCHHHHHHHHCHHHCCEEEECCCCCCCHHHHHHHCCCCCCEEEE
SVKSGEGIELLRTVLLKTVGWQTNIAGEGAYMARQRHLQALLHAKELLKRAETWLHTADQ
EECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHH
LEILAEELRLAQQALSSITGEFTSDDLLGEIFSNFCIGK
HHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA