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The map label for this gene is trmL [H]

Identifier: 114332105

GI number: 114332105

Start: 2268954

End: 2269418

Strand: Direct

Name: trmL [H]

Synonym: Neut_2140

Alternate gene names: 114332105

Gene position: 2268954-2269418 (Clockwise)

Preceding gene: 114332104

Following gene: 114332106

Centisome position: 85.27

GC content: 49.89

Gene sequence:

>465_bases
ATGCTACAGGTAATCCTCCATCAGCCGGAAATCCCGCCAAATACCGGCAATATCATCCGCCTCTGTGCCAACACCAGCAC
ACAGCTGCACCTGATCAAACCACTGGGATTCAACTTGAGTGACCGGCAACTGAAGCGCGCCGGGCTGGATTATCACGAAT
GGGCACGCCTCACCGTGCATGAAGATCTGGCATCCTGCCTGAGCCTCATGGATGGATGCCGGATTTTTGCTGTCACCACC
AAGGCAACAAAGCACTACGATAAAGTGGATTATACGACTGATGATGTCTTTTTGTTTGGCTCTGAAACTTGTGGATTGCC
TGTTGAAGTACTGGAAGATTTCTCTCAGGATCGCCGTATCCGTATTCCTATGGTTCCTTCTTCTCGCAGCCTGAATCTCT
CCAATTCAGCAGCGGTTATTATTTACGAAGCCTGGCGACAAACTGGTTTTGATGGTGGTGTCTAG

Upstream 100 bases:

>100_bases
CAGGCAGTGTTCGCGCCTGGGTTGTCGCCCGGGCATTTCCGAGTTAATCATCCGAAAATAATACGGACCGACAATCATTC
AATCTGACAAACTGATCAAC

Downstream 100 bases:

>100_bases
AGTGTAATATTCATCCGGTTGTTCACCCTCCTCATATCAGGCACTATGGGAATGTCTCCTTACAGCAGTACAAACAGGAC
AGGAAAATGAATCTCCCCTT

Product: RNA methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MLQVILHQPEIPPNTGNIIRLCANTSTQLHLIKPLGFNLSDRQLKRAGLDYHEWARLTVHEDLASCLSLMDGCRIFAVTT
KATKHYDKVDYTTDDVFLFGSETCGLPVEVLEDFSQDRRIRIPMVPSSRSLNLSNSAAVIIYEAWRQTGFDGGV

Sequences:

>Translated_154_residues
MLQVILHQPEIPPNTGNIIRLCANTSTQLHLIKPLGFNLSDRQLKRAGLDYHEWARLTVHEDLASCLSLMDGCRIFAVTT
KATKHYDKVDYTTDDVFLFGSETCGLPVEVLEDFSQDRRIRIPMVPSSRSLNLSNSAAVIIYEAWRQTGFDGGV
>Mature_154_residues
MLQVILHQPEIPPNTGNIIRLCANTSTQLHLIKPLGFNLSDRQLKRAGLDYHEWARLTVHEDLASCLSLMDGCRIFAVTT
KATKHYDKVDYTTDDVFLFGSETCGLPVEVLEDFSQDRRIRIPMVPSSRSLNLSNSAAVIIYEAWRQTGFDGGV

Specific function: Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S- adenosyl-L-methionine to the 2'-OH of the wobble nucleotide [H]

COG id: COG0219

COG function: function code J; Predicted rRNA methylase (SpoU class)

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. TrmL subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790034, Length=154, Percent_Identity=53.2467532467532, Blast_Score=186, Evalue=5e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004440
- InterPro:   IPR016914
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 17307; Mature: 17307

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQVILHQPEIPPNTGNIIRLCANTSTQLHLIKPLGFNLSDRQLKRAGLDYHEWARLTVH
CEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHCCCHHHHEEEEHH
EDLASCLSLMDGCRIFAVTTKATKHYDKVDYTTDDVFLFGSETCGLPVEVLEDFSQDRRI
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCCEE
RIPMVPSSRSLNLSNSAAVIIYEAWRQTGFDGGV
EEEECCCCCCCCCCCCCEEEEEEHHHHCCCCCCC
>Mature Secondary Structure
MLQVILHQPEIPPNTGNIIRLCANTSTQLHLIKPLGFNLSDRQLKRAGLDYHEWARLTVH
CEEEEEECCCCCCCCCCEEEEEECCCCEEEEEECCCCCCCHHHHHHHCCCHHHHEEEEHH
EDLASCLSLMDGCRIFAVTTKATKHYDKVDYTTDDVFLFGSETCGLPVEVLEDFSQDRRI
HHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHCCCCCCEE
RIPMVPSSRSLNLSNSAAVIIYEAWRQTGFDGGV
EEEECCCCCCCCCCCCCEEEEEEHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA