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The map label for this gene is epsB [H]

Identifier: 114332085

GI number: 114332085

Start: 2243620

End: 2245863

Strand: Direct

Name: epsB [H]

Synonym: Neut_2120

Alternate gene names: 114332085

Gene position: 2243620-2245863 (Clockwise)

Preceding gene: 114332084

Following gene: 114332086

Centisome position: 84.31

GC content: 45.45

Gene sequence:

>2244_bases
ATGACTCATTCATTACCTGACATGCCATCTGATCAAAACAGTGACGAAATCGATCTGGGAGAACTGCTGGATATTCTGGC
AGACAACAAGCAACTGATTTTTATCATCACCTTTATCACCTGCATGATCGGTGCTGCCATATCACTTTTAAGCTTGCCGA
TCTATAAGGCGGACAGCTTGTTACAGGTCAGAGAAAATACACAATCCATGGTTGAACAACTGGATTCATTATCAACATTC
TTTGAAACCAAAACGCCGGTTCAAGCTGAAATTGAATTGATCAAGTCCAGAATGATCCTGGGAAAAACAATTAGGAATTT
GAGTCTGGATATTATTGCAACACCCAGGTTTTTTCCGTTCATAGGCGCCGCTGCAGCCAGATGGTTTCAACGCTTCCACG
AGAGCGGCACCCTATCCAATCCACCATCTGAATGGCTATCACATTATGCCTGGGGAGGCGAGGTCATTCAGGTAGATACG
CTTGAAATACCCGACAATTGGAAAAATGAAGAAATTATTTTGCAATCACTCGGCGAAAACCGCTTCGAGCTTATTTATCA
GAATAAAGTCATTCTTGAGGGGGAAGTCGGCAAATTAGCTGCCAAGCAGCCAGAAGGCTCTCTGCACCCAATCAAGCTAT
TTGTTACTCATCTGAAAGCACGTCCCAACACTCATTTCACTCTGATACGCCGCTCCCAAGGAGAAGCAATCCGACAACTA
AAGGAAACTCTCTCGATTGCAGAAATTGGCAAGGGAACCGGCATTCTTCAATTAACCATCGAATCAGACAACCGAGCGAA
AGCTGTTCAAATTGTTAACGAAATTGCCAATACCTATCTTCAGCAGAATGTCGAACAAAAATCAGCAGAATCCCAAAAAA
CCCTTGAATTCCTGGAAAAACAGCTGCCCGCTCTCAAGGAACAACTTGAAACCACTACCAACGCCCTTAACGATTACAGA
ATTCGCGAAGGTTCAATTAATCTCGATCTTGAAACCCAGAATATACTGACAGGTACGGTCGAGCTAAATACCCAGATTAC
ACTTCTGCAACAAAAACGGGATGAACTGAGACAGAGATTTACGGAATCTCATCCTAATGTCGTTGCAATTGACAAGCAGA
TCAGCAGACTGCAGACGCAAATCCATGCACTTGACAAAAAAATAGGCAAACTGCCGGAAACCCAGCAAATCATTCTCAGA
TTATCTCGGGATGTGGAAGTCAATGCCGAGCTCTACACCACCTTGTTGAATCACGCTCAGACGATACGAGTAGCCAAAGC
AGGTACGGTGGGTAATGTAAGAATTGTTGATTACGCTATACCGCCCGATCTACCGATCAAACCCAAAAAAACCCTGATCA
TGGGTATTGCGCTCATGGCAGGATTAATGTTGGGAGTTATATTCGCCTTTATTCGCCGATCACTCAGCCGCGGGGTAGAA
AATCCTGACATCATTGAAAAATCCCTGAATATTCCAGTTTATGCCACGGTTCAGCGCAGTAGACACCAGAAGCTGATCGA
GAAAAAACTAAAAGGCAAACCTGTAGCAGGCAATCATCCTCCAATCATCCTGGCACTGGAAAACAGAGAAGATCTTGCCA
TAGAGAGCCTGCGCAGTTTGCGAACAACCTTACACTTTGCATTTCTGGAGGCGCGTAACAACATCCTCATGATCACCGGC
CCGAGTCCAGAAGCCGGAAAGACATTCATTTCGATGAATCTTGCTGCCGTCATGGCAGATGCCGGCAAAAATATTTTGTT
GATCGATGGAGATCTGCGCAGGGGTGACATCAACAAGTCCATGCGCACAGGAAGAGAGAAAGGCCTGTCCGAATTAATCA
GTCAATCGATCGATCTGAAGGATGCGACCAGATCTGTGCCCCAGGCAAATTTCCACTTCATTCCAACCGGAGCAATACCG
CCTAACCCATCCGAGCTATTACTACACGAGCGATTCGAGCAAATTCTGGAAACTGTCAACAAGCAGTATGATTTGATCAT
CATCGATTCACCTCCCATCCTGGCCGCTACCGACGCGGCCATTATCGGTCGCCTGGCAAGCGTGACATTGATGGTGGTTA
GGGCTGGAACCCATCCGATGCGTGAGCTCGAACAAAGTGTAAAGAAACTTGTACTGGCAGGCGTAAATCTGAAGGGTGTG
ATATTCAATGGCATTCCCGAAACTTCATCACGCCACAGATATACACAGTATAGCTACCAATATGATTATCGCAGCAAGAA
ATGA

Upstream 100 bases:

>100_bases
ATTCATTTGATGGCTCGCTGAGAATTCAACCCTTTCTGAGACACTAGTTACGTGGATATATGCCACACATTACTGCGACA
GGATAGTCATACCCGAATAC

Downstream 100 bases:

>100_bases
CAGAATTCGATCTCCCGAAGCAGCTCGCACTATTTATTATCAGCACGATACTGGCTGGCCTTACCATCCGACTGGCAATT
CCTCTGGCACATATCAGCGG

Product: lipopolysaccharide biosynthesis

Products: ADP; protein tyrosine phosphate [C]

Alternate protein names: EPS I polysaccharide export protein epsB [H]

Number of amino acids: Translated: 747; Mature: 746

Protein sequence:

>747_residues
MTHSLPDMPSDQNSDEIDLGELLDILADNKQLIFIITFITCMIGAAISLLSLPIYKADSLLQVRENTQSMVEQLDSLSTF
FETKTPVQAEIELIKSRMILGKTIRNLSLDIIATPRFFPFIGAAAARWFQRFHESGTLSNPPSEWLSHYAWGGEVIQVDT
LEIPDNWKNEEIILQSLGENRFELIYQNKVILEGEVGKLAAKQPEGSLHPIKLFVTHLKARPNTHFTLIRRSQGEAIRQL
KETLSIAEIGKGTGILQLTIESDNRAKAVQIVNEIANTYLQQNVEQKSAESQKTLEFLEKQLPALKEQLETTTNALNDYR
IREGSINLDLETQNILTGTVELNTQITLLQQKRDELRQRFTESHPNVVAIDKQISRLQTQIHALDKKIGKLPETQQIILR
LSRDVEVNAELYTTLLNHAQTIRVAKAGTVGNVRIVDYAIPPDLPIKPKKTLIMGIALMAGLMLGVIFAFIRRSLSRGVE
NPDIIEKSLNIPVYATVQRSRHQKLIEKKLKGKPVAGNHPPIILALENREDLAIESLRSLRTTLHFAFLEARNNILMITG
PSPEAGKTFISMNLAAVMADAGKNILLIDGDLRRGDINKSMRTGREKGLSELISQSIDLKDATRSVPQANFHFIPTGAIP
PNPSELLLHERFEQILETVNKQYDLIIIDSPPILAATDAAIIGRLASVTLMVVRAGTHPMRELEQSVKKLVLAGVNLKGV
IFNGIPETSSRHRYTQYSYQYDYRSKK

Sequences:

>Translated_747_residues
MTHSLPDMPSDQNSDEIDLGELLDILADNKQLIFIITFITCMIGAAISLLSLPIYKADSLLQVRENTQSMVEQLDSLSTF
FETKTPVQAEIELIKSRMILGKTIRNLSLDIIATPRFFPFIGAAAARWFQRFHESGTLSNPPSEWLSHYAWGGEVIQVDT
LEIPDNWKNEEIILQSLGENRFELIYQNKVILEGEVGKLAAKQPEGSLHPIKLFVTHLKARPNTHFTLIRRSQGEAIRQL
KETLSIAEIGKGTGILQLTIESDNRAKAVQIVNEIANTYLQQNVEQKSAESQKTLEFLEKQLPALKEQLETTTNALNDYR
IREGSINLDLETQNILTGTVELNTQITLLQQKRDELRQRFTESHPNVVAIDKQISRLQTQIHALDKKIGKLPETQQIILR
LSRDVEVNAELYTTLLNHAQTIRVAKAGTVGNVRIVDYAIPPDLPIKPKKTLIMGIALMAGLMLGVIFAFIRRSLSRGVE
NPDIIEKSLNIPVYATVQRSRHQKLIEKKLKGKPVAGNHPPIILALENREDLAIESLRSLRTTLHFAFLEARNNILMITG
PSPEAGKTFISMNLAAVMADAGKNILLIDGDLRRGDINKSMRTGREKGLSELISQSIDLKDATRSVPQANFHFIPTGAIP
PNPSELLLHERFEQILETVNKQYDLIIIDSPPILAATDAAIIGRLASVTLMVVRAGTHPMRELEQSVKKLVLAGVNLKGV
IFNGIPETSSRHRYTQYSYQYDYRSKK
>Mature_746_residues
THSLPDMPSDQNSDEIDLGELLDILADNKQLIFIITFITCMIGAAISLLSLPIYKADSLLQVRENTQSMVEQLDSLSTFF
ETKTPVQAEIELIKSRMILGKTIRNLSLDIIATPRFFPFIGAAAARWFQRFHESGTLSNPPSEWLSHYAWGGEVIQVDTL
EIPDNWKNEEIILQSLGENRFELIYQNKVILEGEVGKLAAKQPEGSLHPIKLFVTHLKARPNTHFTLIRRSQGEAIRQLK
ETLSIAEIGKGTGILQLTIESDNRAKAVQIVNEIANTYLQQNVEQKSAESQKTLEFLEKQLPALKEQLETTTNALNDYRI
REGSINLDLETQNILTGTVELNTQITLLQQKRDELRQRFTESHPNVVAIDKQISRLQTQIHALDKKIGKLPETQQIILRL
SRDVEVNAELYTTLLNHAQTIRVAKAGTVGNVRIVDYAIPPDLPIKPKKTLIMGIALMAGLMLGVIFAFIRRSLSRGVEN
PDIIEKSLNIPVYATVQRSRHQKLIEKKLKGKPVAGNHPPIILALENREDLAIESLRSLRTTLHFAFLEARNNILMITGP
SPEAGKTFISMNLAAVMADAGKNILLIDGDLRRGDINKSMRTGREKGLSELISQSIDLKDATRSVPQANFHFIPTGAIPP
NPSELLLHERFEQILETVNKQYDLIIIDSPPILAATDAAIIGRLASVTLMVVRAGTHPMRELEQSVKKLVLAGVNLKGVI
FNGIPETSSRHRYTQYSYQYDYRSKK

Specific function: Probably involved in polymerization and/or export of exopolysaccharide EPS I which functions as a virulence factor. May be involved in an ATP-dependent process in the pathway for EPS I production, possibly export of the trimeric repeat units across the in

COG id: COG3206

COG function: function code M; Uncharacterized protein involved in exopolysaccharide biosynthesis

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the etk/wzc family [H]

Homologues:

Organism=Escherichia coli, GI87082032, Length=734, Percent_Identity=36.7847411444142, Blast_Score=474, Evalue=1e-134,
Organism=Escherichia coli, GI1787216, Length=738, Percent_Identity=36.8563685636856, Blast_Score=465, Evalue=1e-132,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR005702
- InterPro:   IPR005700
- InterPro:   IPR003856 [H]

Pfam domain/function: PF01656 CbiA; PF02706 Wzz [H]

EC number: 2.7.1.112 [C]

Molecular weight: Translated: 83746; Mature: 83615

Theoretical pI: Translated: 8.37; Mature: 8.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTHSLPDMPSDQNSDEIDLGELLDILADNKQLIFIITFITCMIGAAISLLSLPIYKADSL
CCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHH
LQVRENTQSMVEQLDSLSTFFETKTPVQAEIELIKSRMILGKTIRNLSLDIIATPRFFPF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHH
IGAAAARWFQRFHESGTLSNPPSEWLSHYAWGGEVIQVDTLEIPDNWKNEEIILQSLGEN
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHCCCC
RFELIYQNKVILEGEVGKLAAKQPEGSLHPIKLFVTHLKARPNTHFTLIRRSQGEAIRQL
CEEEEEECEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
KETLSIAEIGKGTGILQLTIESDNRAKAVQIVNEIANTYLQQNVEQKSAESQKTLEFLEK
HHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLPALKEQLETTTNALNDYRIREGSINLDLETQNILTGTVELNTQITLLQQKRDELRQRF
HHHHHHHHHHHHHHHHHCCEEECCCEEEEEECCCEEEEEEEECCEEEHHHHHHHHHHHHH
TESHPNVVAIDKQISRLQTQIHALDKKIGKLPETQQIILRLSRDVEVNAELYTTLLNHAQ
HHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
TIRVAKAGTVGNVRIVDYAIPPDLPIKPKKTLIMGIALMAGLMLGVIFAFIRRSLSRGVE
EEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NPDIIEKSLNIPVYATVQRSRHQKLIEKKLKGKPVAGNHPPIILALENREDLAIESLRSL
CHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
RTTLHFAFLEARNNILMITGPSPEAGKTFISMNLAAVMADAGKNILLIDGDLRRGDINKS
HHHHHHHHHCCCCCEEEEECCCCCCCCEEEEHHHHHHHHCCCCEEEEEECCCCCCCCCHH
MRTGREKGLSELISQSIDLKDATRSVPQANFHFIPTGAIPPNPSELLLHERFEQILETVN
HHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
KQYDLIIIDSPPILAATDAAIIGRLASVTLMVVRAGTHPMRELEQSVKKLVLAGVNLKGV
CCCCEEEECCCCCEEECHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCEE
IFNGIPETSSRHRYTQYSYQYDYRSKK
EEECCCCCCCCCCEEEEEEEECCCCCC
>Mature Secondary Structure 
THSLPDMPSDQNSDEIDLGELLDILADNKQLIFIITFITCMIGAAISLLSLPIYKADSL
CCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHCCCCCCCHH
LQVRENTQSMVEQLDSLSTFFETKTPVQAEIELIKSRMILGKTIRNLSLDIIATPRFFPF
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCHHHH
IGAAAARWFQRFHESGTLSNPPSEWLSHYAWGGEVIQVDTLEIPDNWKNEEIILQSLGEN
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHCCCC
RFELIYQNKVILEGEVGKLAAKQPEGSLHPIKLFVTHLKARPNTHFTLIRRSQGEAIRQL
CEEEEEECEEEEECCCCCHHCCCCCCCCCHHHHHHHHHHCCCCCEEEEEECCCCHHHHHH
KETLSIAEIGKGTGILQLTIESDNRAKAVQIVNEIANTYLQQNVEQKSAESQKTLEFLEK
HHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QLPALKEQLETTTNALNDYRIREGSINLDLETQNILTGTVELNTQITLLQQKRDELRQRF
HHHHHHHHHHHHHHHHHCCEEECCCEEEEEECCCEEEEEEEECCEEEHHHHHHHHHHHHH
TESHPNVVAIDKQISRLQTQIHALDKKIGKLPETQQIILRLSRDVEVNAELYTTLLNHAQ
HHCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHH
TIRVAKAGTVGNVRIVDYAIPPDLPIKPKKTLIMGIALMAGLMLGVIFAFIRRSLSRGVE
EEEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
NPDIIEKSLNIPVYATVQRSRHQKLIEKKLKGKPVAGNHPPIILALENREDLAIESLRSL
CHHHHHHHCCCCEEEEHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHHHHHHHH
RTTLHFAFLEARNNILMITGPSPEAGKTFISMNLAAVMADAGKNILLIDGDLRRGDINKS
HHHHHHHHHCCCCCEEEEECCCCCCCCEEEEHHHHHHHHCCCCEEEEEECCCCCCCCCHH
MRTGREKGLSELISQSIDLKDATRSVPQANFHFIPTGAIPPNPSELLLHERFEQILETVN
HHHHHHHHHHHHHHHCCCCCHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHC
KQYDLIIIDSPPILAATDAAIIGRLASVTLMVVRAGTHPMRELEQSVKKLVLAGVNLKGV
CCCCEEEECCCCCEEECHHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCEE
IFNGIPETSSRHRYTQYSYQYDYRSKK
EEECCCCCCCCCCEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; a protein tyrosine [C]

Specific reaction: ATP + a protein tyrosine = ADP + protein tyrosine phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]