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The map label for this gene is rfbX [H]

Identifier: 114332082

GI number: 114332082

Start: 2240426

End: 2241667

Strand: Reverse

Name: rfbX [H]

Synonym: Neut_2117

Alternate gene names: 114332082

Gene position: 2241667-2240426 (Counterclockwise)

Preceding gene: 114332087

Following gene: 114332081

Centisome position: 84.24

GC content: 40.66

Gene sequence:

>1242_bases
TTGGCTGCTACCTCCAGAAAAAAAAATGATTTTCGCAGAGTACTGAGTAATCTGTTATCGCTTGCGGCGGTGCGGGGTCT
GGATTATCTGGTGCCATTGATTACCCTGCCATATTTGGTTCGTGTGTTGGGGATAGAAGGTTTCGGCCTGATCAATTATG
CCTTGTCTTTCGCTTTGTATTTTTCAGCAGTGATGCAGTATGGATTCTCGATAACAGCGGTTAGAAAAATTGCACAAAAT
CGTGATAATCCTGAAGAGATTTCTAGTATTTATAGCGAGACTATCACCGCCATCCTGTTGCTCGTTGTAGCAAGCGCACT
GATCTATTTTCCGATAGTGCTACTCATAAATGATTTTCATCAGCACTTTTTGCTATACGCATTCTCATTCTGCTTTGTTT
CTGCACAAGCATTATTTCCTCTCTGGTTCTTCCAGGGGGTAGAGCAAATGCGACAAAGTGCCATTATCAGCATTACATCA
AAGTTCTTGATGTTGGCTGGTATTTTTATATTTATCAGAGACGAGTCAGATTACTACCGGGTTCCGATGTTATATGCAGT
AGCCATGGTGGGGTGTGCAGTCTTTTCTGTGCTCTGGATTGAGAGTCGATACAAGATTACATATAAAGTACCCTCACTAA
TAAAAGTAAAAAATGCATATAGAGAGGGAGCAAATATTTTTGTCACCCAGCTGACACCAAATTTGTATAACAATTCTGCT
TTTTTTCTACTGGGGATTTTTGAAGGACCAATAGCGGTAGGATTTTTCAGTGCAGCAGCAAAAATAATCGATGTATTCAA
TTCCGCAGGAATCATAATTTCTAGCGCATTCTTTCCATATTTAGCAAGAAACAGGAGTGTAATACCAAAGTTTCATAAGT
TAATGTTGATAGCGGGCGTTCTTCTATTCGTACTTTGCTTTATTTGTTCTGATTTTCTTGTGGCGATTTTGTTTTCTGAT
AAGGGGATCGGGGTTGCTTATTACGTCAAGTTGATGAGCTGCGGGATTCTTGCCTATTTCATGATCCTTTCATTTCACAA
TAATGGCTTGGTGCTAGCGGGAAAAGATAAATACGTTCGTCTGATTGCCACATGGACGTCTGGTCTGTTCTTCTTGTTGG
GGCTGGGTATCGTTCCGACCTTTGGTATTTATGGTGCAGTGATCATGTTGGTGGGTGCGCGGGTGATAATGGCAACGTGG
GGATTCTTTTTTTATAGAAAATATGCTGCAAACATTCTTTAG

Upstream 100 bases:

>100_bases
AAAACAGATATGATGCAGTGCTAAAACAAAAGGGATGTAACAAGTATATTTGGTATTGTACCGAGCTGATAATTCACTGA
ACTGATCCCGATCTATTTCT

Downstream 100 bases:

>100_bases
ATTTGGAAATTTACTATGAAAAAGATAGCCATACATAACCGCCCTGGGAGCTTTAGTGATCGTTGGATGGTGTATTGTCG
ACAAAAGAATATTCCATACA

Product: polysaccharide biosynthesis protein

Products: lipopolysaccharide [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 413; Mature: 412

Protein sequence:

>413_residues
MAATSRKKNDFRRVLSNLLSLAAVRGLDYLVPLITLPYLVRVLGIEGFGLINYALSFALYFSAVMQYGFSITAVRKIAQN
RDNPEEISSIYSETITAILLLVVASALIYFPIVLLINDFHQHFLLYAFSFCFVSAQALFPLWFFQGVEQMRQSAIISITS
KFLMLAGIFIFIRDESDYYRVPMLYAVAMVGCAVFSVLWIESRYKITYKVPSLIKVKNAYREGANIFVTQLTPNLYNNSA
FFLLGIFEGPIAVGFFSAAAKIIDVFNSAGIIISSAFFPYLARNRSVIPKFHKLMLIAGVLLFVLCFICSDFLVAILFSD
KGIGVAYYVKLMSCGILAYFMILSFHNNGLVLAGKDKYVRLIATWTSGLFFLLGLGIVPTFGIYGAVIMLVGARVIMATW
GFFFYRKYAANIL

Sequences:

>Translated_413_residues
MAATSRKKNDFRRVLSNLLSLAAVRGLDYLVPLITLPYLVRVLGIEGFGLINYALSFALYFSAVMQYGFSITAVRKIAQN
RDNPEEISSIYSETITAILLLVVASALIYFPIVLLINDFHQHFLLYAFSFCFVSAQALFPLWFFQGVEQMRQSAIISITS
KFLMLAGIFIFIRDESDYYRVPMLYAVAMVGCAVFSVLWIESRYKITYKVPSLIKVKNAYREGANIFVTQLTPNLYNNSA
FFLLGIFEGPIAVGFFSAAAKIIDVFNSAGIIISSAFFPYLARNRSVIPKFHKLMLIAGVLLFVLCFICSDFLVAILFSD
KGIGVAYYVKLMSCGILAYFMILSFHNNGLVLAGKDKYVRLIATWTSGLFFLLGLGIVPTFGIYGAVIMLVGARVIMATW
GFFFYRKYAANIL
>Mature_412_residues
AATSRKKNDFRRVLSNLLSLAAVRGLDYLVPLITLPYLVRVLGIEGFGLINYALSFALYFSAVMQYGFSITAVRKIAQNR
DNPEEISSIYSETITAILLLVVASALIYFPIVLLINDFHQHFLLYAFSFCFVSAQALFPLWFFQGVEQMRQSAIISITSK
FLMLAGIFIFIRDESDYYRVPMLYAVAMVGCAVFSVLWIESRYKITYKVPSLIKVKNAYREGANIFVTQLTPNLYNNSAF
FLLGIFEGPIAVGFFSAAAKIIDVFNSAGIIISSAFFPYLARNRSVIPKFHKLMLIAGVLLFVLCFICSDFLVAILFSDK
GIGVAYYVKLMSCGILAYFMILSFHNNGLVLAGKDKYVRLIATWTSGLFFLLGLGIVPTFGIYGAVIMLVGARVIMATWG
FFFYRKYAANIL

Specific function: May be involved in the translocation process of the nascent O-polysaccharide molecules and/or its ligation to lipid A core units [H]

COG id: COG2244

COG function: function code R; Membrane protein involved in the export of O-antigen and teichoic acid

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polysaccharide synthase family [H]

Homologues:

Organism=Escherichia coli, GI1788349, Length=279, Percent_Identity=28.673835125448, Blast_Score=131, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002797 [H]

Pfam domain/function: PF01943 Polysacc_synt [H]

EC number: NA

Molecular weight: Translated: 46278; Mature: 46147

Theoretical pI: Translated: 9.74; Mature: 9.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAATSRKKNDFRRVLSNLLSLAAVRGLDYLVPLITLPYLVRVLGIEGFGLINYALSFALY
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FSAVMQYGFSITAVRKIAQNRDNPEEISSIYSETITAILLLVVASALIYFPIVLLINDFH
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QHFLLYAFSFCFVSAQALFPLWFFQGVEQMRQSAIISITSKFLMLAGIFIFIRDESDYYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCH
VPMLYAVAMVGCAVFSVLWIESRYKITYKVPSLIKVKNAYREGANIFVTQLTPNLYNNSA
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCCE
FFLLGIFEGPIAVGFFSAAAKIIDVFNSAGIIISSAFFPYLARNRSVIPKFHKLMLIAGV
EEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LLFVLCFICSDFLVAILFSDKGIGVAYYVKLMSCGILAYFMILSFHNNGLVLAGKDKYVR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEE
LIATWTSGLFFLLGLGIVPTFGIYGAVIMLVGARVIMATWGFFFYRKYAANIL
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
AATSRKKNDFRRVLSNLLSLAAVRGLDYLVPLITLPYLVRVLGIEGFGLINYALSFALY
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHH
FSAVMQYGFSITAVRKIAQNRDNPEEISSIYSETITAILLLVVASALIYFPIVLLINDFH
HHHHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QHFLLYAFSFCFVSAQALFPLWFFQGVEQMRQSAIISITSKFLMLAGIFIFIRDESDYYR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEECCCCCCH
VPMLYAVAMVGCAVFSVLWIESRYKITYKVPSLIKVKNAYREGANIFVTQLTPNLYNNSA
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHCCCCEEEEEECCCCCCCCE
FFLLGIFEGPIAVGFFSAAAKIIDVFNSAGIIISSAFFPYLARNRSVIPKFHKLMLIAGV
EEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
LLFVLCFICSDFLVAILFSDKGIGVAYYVKLMSCGILAYFMILSFHNNGLVLAGKDKYVR
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEE
LIATWTSGLFFLLGLGIVPTFGIYGAVIMLVGARVIMATWGFFFYRKYAANIL
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: lipopolysaccharide [Cytoplasm] [C]

Specific reaction: lipopolysaccharide [Cytoplasm] = lipopolysaccharide [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7517390; 7517391; 9097040; 9278503 [H]