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The map label for this gene is hisA [H]

Identifier: 114331883

GI number: 114331883

Start: 2022476

End: 2023222

Strand: Direct

Name: hisA [H]

Synonym: Neut_1908

Alternate gene names: 114331883

Gene position: 2022476-2023222 (Clockwise)

Preceding gene: 114331882

Following gene: 114331884

Centisome position: 76.0

GC content: 50.07

Gene sequence:

>747_bases
ATGCTGATTATTCCTGCTATAGATCTGAAAGACGGACAATGCGTCCGCCTGAAGCAAGGCATCATGGAAGATGCCACAGT
ATTTTCAGAAACCCCTGAAACAGTTGCACTGCACTGGCTGGATAACGGAGCCCGTCAGCTGCATCTGGTTGACCTTAATG
GTGCTTTTGCCGGAAAACCTAAAAATGGTGAAGCTATCCGCGCGATAGTCGAAGCCATTGATGGAAGAATTCCAATCCAG
CTGGGCGGAGGAATCCGCGATCTGGAAACTGTCGAGTACTATCTGGATAACGGCATCAGCTACGTAATCATTGGAACGGC
TGCGGTCAAGATACCTGGCTTCCTGCACGATGCCTGCTATGCGTTTCCAGGTCAGATTATGGTCGGGCTGGATGCCAAAG
GTGGAAAAGTGGCTGTTGACGGCTGGTCCAAAGTAACCGGCCATGACGTTATTGATCTTGCAAAAAAATTCCAGGATTAC
GGTGTCGAAGCAATTATTCACACCGATATCGGTCGTGATGGCATGCTCAACGGCCTCAATATCGAAGCTACTGTGGAACT
CGCTCAAGCGCTGACAATTCCCGTCATTGCAAGTGGCGGTGTGACCAACCTGGATGACATCAGGAAATTGTGCCAAGTCC
AGTCCGAAGGAATCACCGGTGTCATCACTGGCCGGGCAATTTATCAGGGCTCCCTTGATTTCAAGGAAGCACAAGCCTTG
GCAGATCAGCTGGATTCAGCAAAGTGA

Upstream 100 bases:

>100_bases
AAAATGGATACCATAACAACATTCCAGCATTATCCTATCCAGCATTTCCATTGTATTATCAATTTTTTTCAAGTCTGATC
ATTAACCCCGATAACTTCGT

Downstream 100 bases:

>100_bases
TTGACATCACTCACTCACAGAAACTCACTGGCTTTGTGCTTTTTATCCCCTTCTTTTTTTATCTAATTTATTTCTGATAA
CTTTGACCAATGGGATTAGC

Product: 1-(5-phosphoribosyl)-5-[(5- phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase

Products: NA

Alternate protein names: Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase [H]

Number of amino acids: Translated: 248; Mature: 248

Protein sequence:

>248_residues
MLIIPAIDLKDGQCVRLKQGIMEDATVFSETPETVALHWLDNGARQLHLVDLNGAFAGKPKNGEAIRAIVEAIDGRIPIQ
LGGGIRDLETVEYYLDNGISYVIIGTAAVKIPGFLHDACYAFPGQIMVGLDAKGGKVAVDGWSKVTGHDVIDLAKKFQDY
GVEAIIHTDIGRDGMLNGLNIEATVELAQALTIPVIASGGVTNLDDIRKLCQVQSEGITGVITGRAIYQGSLDFKEAQAL
ADQLDSAK

Sequences:

>Translated_248_residues
MLIIPAIDLKDGQCVRLKQGIMEDATVFSETPETVALHWLDNGARQLHLVDLNGAFAGKPKNGEAIRAIVEAIDGRIPIQ
LGGGIRDLETVEYYLDNGISYVIIGTAAVKIPGFLHDACYAFPGQIMVGLDAKGGKVAVDGWSKVTGHDVIDLAKKFQDY
GVEAIIHTDIGRDGMLNGLNIEATVELAQALTIPVIASGGVTNLDDIRKLCQVQSEGITGVITGRAIYQGSLDFKEAQAL
ADQLDSAK
>Mature_248_residues
MLIIPAIDLKDGQCVRLKQGIMEDATVFSETPETVALHWLDNGARQLHLVDLNGAFAGKPKNGEAIRAIVEAIDGRIPIQ
LGGGIRDLETVEYYLDNGISYVIIGTAAVKIPGFLHDACYAFPGQIMVGLDAKGGKVAVDGWSKVTGHDVIDLAKKFQDY
GVEAIIHTDIGRDGMLNGLNIEATVELAQALTIPVIASGGVTNLDDIRKLCQVQSEGITGVITGRAIYQGSLDFKEAQAL
ADQLDSAK

Specific function: Histidine biosynthesis; fourth step. [C]

COG id: COG0106

COG function: function code E; Phosphoribosylformimino-5-aminoimidazole carboxamide ribonucleotide (ProFAR) isomerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hisA/hisF family [H]

Homologues:

Organism=Escherichia coli, GI87082028, Length=245, Percent_Identity=33.8775510204082, Blast_Score=119, Evalue=2e-28,
Organism=Escherichia coli, GI1788336, Length=214, Percent_Identity=27.1028037383178, Blast_Score=71, Evalue=9e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR006062
- InterPro:   IPR006063
- InterPro:   IPR023016
- InterPro:   IPR011060 [H]

Pfam domain/function: PF00977 His_biosynth [H]

EC number: =5.3.1.16 [H]

Molecular weight: Translated: 26425; Mature: 26425

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIPAIDLKDGQCVRLKQGIMEDATVFSETPETVALHWLDNGARQLHLVDLNGAFAGKP
CEEEEEEECCCCCEEHHHHCCHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCCC
KNGEAIRAIVEAIDGRIPIQLGGGIRDLETVEYYLDNGISYVIIGTAAVKIPGFLHDACY
CCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHH
AFPGQIMVGLDAKGGKVAVDGWSKVTGHDVIDLAKKFQDYGVEAIIHTDIGRDGMLNGLN
HCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
IEATVELAQALTIPVIASGGVTNLDDIRKLCQVQSEGITGVITGRAIYQGSLDFKEAQAL
CHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCEEEECCCCHHHHHHH
ADQLDSAK
HHHHCCCC
>Mature Secondary Structure
MLIIPAIDLKDGQCVRLKQGIMEDATVFSETPETVALHWLDNGARQLHLVDLNGAFAGKP
CEEEEEEECCCCCEEHHHHCCHHHHHHHCCCCCEEEEEEECCCCCEEEEEEECCCCCCCC
KNGEAIRAIVEAIDGRIPIQLGGGIRDLETVEYYLDNGISYVIIGTAAVKIPGFLHDACY
CCCHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCEEEEEEEEEEECCCHHHHHHH
AFPGQIMVGLDAKGGKVAVDGWSKVTGHDVIDLAKKFQDYGVEAIIHTDIGRDGMLNGLN
HCCCEEEEEEECCCCEEEECCCCCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
IEATVELAQALTIPVIASGGVTNLDDIRKLCQVQSEGITGVITGRAIYQGSLDFKEAQAL
CHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCCCEEEEECCEEEECCCCHHHHHHH
ADQLDSAK
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]