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The map label for this gene is miaB

Identifier: 114331766

GI number: 114331766

Start: 1893167

End: 1894498

Strand: Reverse

Name: miaB

Synonym: Neut_1789

Alternate gene names: 114331766

Gene position: 1894498-1893167 (Counterclockwise)

Preceding gene: 114331767

Following gene: 114331765

Centisome position: 71.19

GC content: 41.97

Gene sequence:

>1332_bases
ATGAGTAGTAAGCTTTATATCAAAACATTTGGCTGTCAGATGAATGAATACGATTCAGCCAAAATGGCTGATATTCTTTT
ATCAGAAAAAAATATGGAGCTGACTGAAGTACCTGAAGAAGCTGATTTAATTCTATTTAATACTTGCTCAGTTCGTGAAA
AGGCGCAGGAAAAAGTTTTTCATGATCTGGGGCGCGTGCGGCATCTTAAAAATAGCAAGCCTGATTTGTTGATTGGTGTT
GGTGGATGTGTGGCCAGCCAGGAAGGTTCAGAGATAGTCAGGCGTGCTCCATTTGTAGATTTGGTATTTGGCCCGCAAAC
ATTACACAGATTACCCGAGTTGATTGATGCTCGCAGGCGTACAGGCCAGTCACAGGTTGACATTACTTTTCCAGAAATAG
AAAAATTTGATCGTTTGCCACCTGCTCGTACAACAGGAGCAACTGCTTTTGTATCAATTATGGAAGGATGCAGTAAATAC
TGTAGCTTTTGTGTTGTTCCTTATACCCGGGGAGAGGAGGTTTCCAGGCCATTGGATGATGTTCTGACCGAAGTGGCTGG
GCTTGTGATTCAAGGGGTCAAGGAAGTTACTCTGCTCGGACAAAATGTGAATGCTTATTACGATAAGACAAGCGGAGAAG
GAGATATAGATTTTGCTACGCTACTGGATTATATCCATGAAATTCCGGGCTTGGTGCGTATCCGGTATACAACTTCCCAT
CCGAGAGAATTTACAGCCCGATTGATTGAAACGTATCAGCGATTACCGAAGCTGGTTGGTCATGTGCACTTGCCCATACA
ATCCGGATCAGACAGGATTCTGGCTGCGATGAAACGAGGTTATACTATAATTGAGTATAAATCCATCATCCGTAAATTAC
GTACGATTCGTCCAAATATATCAATATCATCAGATTTTATTGTCGGATTTCCGGGAGAAACGGATACTGATTTTGAAGAG
ACGATGAAACTGATTGATGATGTGAAATTTGATGAATCTTTTAGTTTTATTTATAGCCCGCGTCCTGGAACACCTGCCTC
AGATTTACCGGATGACACGCCTTATCGGATAAAGCTGGCCAGACTGCATCAGTTGCAGGAAAAAATACAGCGTAATGCAC
AAATGATCAGTCAGAGTATGGTTGATACTATCCAGCGTGTTTTGGTAGAGGGCCCATCAAAAAAAGATCCAAATGAATTT
TGTGGAAGAACGGATAATAATCGGGTAGTCAATTTTGCTGGACACGCTGGTTTGGTGGGAAGCTTTGTTGATATCAAGAT
AACAGCAGTCTCATCGCATACGTTACGCGGTGAAATTTCTGATATGCAATGA

Upstream 100 bases:

>100_bases
GGGCTAGGCTGATTAAGATCATCGGTGGGCTGAGTACCACAGTAATTAATATCTCGTTGCTTGCAGCGAGTGTTTTTTTA
GGTTGATGGTAAGGAATATA

Downstream 100 bases:

>100_bases
ACAATCTTGTTACGGCCGGTTTGTTGCTCAACTTGATTGCAATTAGAGTTTGAACGCTGCGATGACGTTTTCGGTAGAAT
CGAAACCTGAATCTGTTGAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 443; Mature: 442

Protein sequence:

>443_residues
MSSKLYIKTFGCQMNEYDSAKMADILLSEKNMELTEVPEEADLILFNTCSVREKAQEKVFHDLGRVRHLKNSKPDLLIGV
GGCVASQEGSEIVRRAPFVDLVFGPQTLHRLPELIDARRRTGQSQVDITFPEIEKFDRLPPARTTGATAFVSIMEGCSKY
CSFCVVPYTRGEEVSRPLDDVLTEVAGLVIQGVKEVTLLGQNVNAYYDKTSGEGDIDFATLLDYIHEIPGLVRIRYTTSH
PREFTARLIETYQRLPKLVGHVHLPIQSGSDRILAAMKRGYTIIEYKSIIRKLRTIRPNISISSDFIVGFPGETDTDFEE
TMKLIDDVKFDESFSFIYSPRPGTPASDLPDDTPYRIKLARLHQLQEKIQRNAQMISQSMVDTIQRVLVEGPSKKDPNEF
CGRTDNNRVVNFAGHAGLVGSFVDIKITAVSSHTLRGEISDMQ

Sequences:

>Translated_443_residues
MSSKLYIKTFGCQMNEYDSAKMADILLSEKNMELTEVPEEADLILFNTCSVREKAQEKVFHDLGRVRHLKNSKPDLLIGV
GGCVASQEGSEIVRRAPFVDLVFGPQTLHRLPELIDARRRTGQSQVDITFPEIEKFDRLPPARTTGATAFVSIMEGCSKY
CSFCVVPYTRGEEVSRPLDDVLTEVAGLVIQGVKEVTLLGQNVNAYYDKTSGEGDIDFATLLDYIHEIPGLVRIRYTTSH
PREFTARLIETYQRLPKLVGHVHLPIQSGSDRILAAMKRGYTIIEYKSIIRKLRTIRPNISISSDFIVGFPGETDTDFEE
TMKLIDDVKFDESFSFIYSPRPGTPASDLPDDTPYRIKLARLHQLQEKIQRNAQMISQSMVDTIQRVLVEGPSKKDPNEF
CGRTDNNRVVNFAGHAGLVGSFVDIKITAVSSHTLRGEISDMQ
>Mature_442_residues
SSKLYIKTFGCQMNEYDSAKMADILLSEKNMELTEVPEEADLILFNTCSVREKAQEKVFHDLGRVRHLKNSKPDLLIGVG
GCVASQEGSEIVRRAPFVDLVFGPQTLHRLPELIDARRRTGQSQVDITFPEIEKFDRLPPARTTGATAFVSIMEGCSKYC
SFCVVPYTRGEEVSRPLDDVLTEVAGLVIQGVKEVTLLGQNVNAYYDKTSGEGDIDFATLLDYIHEIPGLVRIRYTTSHP
REFTARLIETYQRLPKLVGHVHLPIQSGSDRILAAMKRGYTIIEYKSIIRKLRTIRPNISISSDFIVGFPGETDTDFEET
MKLIDDVKFDESFSFIYSPRPGTPASDLPDDTPYRIKLARLHQLQEKIQRNAQMISQSMVDTIQRVLVEGPSKKDPNEFC
GRTDNNRVVNFAGHAGLVGSFVDIKITAVSSHTLRGEISDMQ

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=476, Percent_Identity=37.8151260504202, Blast_Score=312, Evalue=3e-85,
Organism=Homo sapiens, GI28872782, Length=476, Percent_Identity=37.8151260504202, Blast_Score=312, Evalue=4e-85,
Organism=Homo sapiens, GI93277076, Length=457, Percent_Identity=27.5711159737418, Blast_Score=168, Evalue=1e-41,
Organism=Escherichia coli, GI1786882, Length=443, Percent_Identity=62.3024830699774, Blast_Score=563, Evalue=1e-162,
Organism=Escherichia coli, GI1787057, Length=456, Percent_Identity=27.1929824561404, Blast_Score=151, Evalue=9e-38,
Organism=Caenorhabditis elegans, GI17553146, Length=460, Percent_Identity=35, Blast_Score=264, Evalue=8e-71,
Organism=Caenorhabditis elegans, GI71996771, Length=371, Percent_Identity=26.9541778975741, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356207, Length=474, Percent_Identity=33.9662447257384, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI19922432, Length=454, Percent_Identity=28.1938325991189, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_NITEC (Q0AF59)

Other databases:

- EMBL:   CP000450
- RefSeq:   YP_747988.1
- ProteinModelPortal:   Q0AF59
- SMR:   Q0AF59
- STRING:   Q0AF59
- GeneID:   4272267
- GenomeReviews:   CP000450_GR
- KEGG:   net:Neut_1789
- NMPDR:   fig|335283.3.peg.1997
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- PhylomeDB:   Q0AF59
- ProtClustDB:   PRK14325
- BioCyc:   NEUT335283:NEUT_1789-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49757; Mature: 49626

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSKLYIKTFGCQMNEYDSAKMADILLSEKNMELTEVPEEADLILFNTCSVREKAQEKVF
CCCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCEEEEECCCHHHHHHHHHH
HDLGRVRHLKNSKPDLLIGVGGCVASQEGSEIVRRAPFVDLVFGPQTLHRLPELIDARRR
HHHHHHHHHCCCCCCEEEECCCHHCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHH
TGQSQVDITFPEIEKFDRLPPARTTGATAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD
CCCCEEEEECHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHH
VLTEVAGLVIQGVKEVTLLGQNVNAYYDKTSGEGDIDFATLLDYIHEIPGLVRIRYTTSH
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEECCC
PREFTARLIETYQRLPKLVGHVHLPIQSGSDRILAAMKRGYTIIEYKSIIRKLRTIRPNI
CHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHCCCEEEHHHHHHHHHHHCCCCC
SISSDFIVGFPGETDTDFEETMKLIDDVKFDESFSFIYSPRPGTPASDLPDDTPYRIKLA
EECCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCEEHHH
RLHQLQEKIQRNAQMISQSMVDTIQRVLVEGPSKKDPNEFCGRTDNNRVVNFAGHAGLVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEECCCCCCEE
SFVDIKITAVSSHTLRGEISDMQ
CEEEEEEEEEECCEECCCCCCCC
>Mature Secondary Structure 
SSKLYIKTFGCQMNEYDSAKMADILLSEKNMELTEVPEEADLILFNTCSVREKAQEKVF
CCCEEEEEECCCCCCCCCHHHHHHHHCCCCCCHHCCCCCCCEEEEECCCHHHHHHHHHH
HDLGRVRHLKNSKPDLLIGVGGCVASQEGSEIVRRAPFVDLVFGPQTLHRLPELIDARRR
HHHHHHHHHCCCCCCEEEECCCHHCCCCHHHHHHHCCCEEEEECHHHHHHHHHHHHHHHH
TGQSQVDITFPEIEKFDRLPPARTTGATAFVSIMEGCSKYCSFCVVPYTRGEEVSRPLDD
CCCCEEEEECHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCEEEEECCCCCHHCCCHHH
VLTEVAGLVIQGVKEVTLLGQNVNAYYDKTSGEGDIDFATLLDYIHEIPGLVRIRYTTSH
HHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEECCC
PREFTARLIETYQRLPKLVGHVHLPIQSGSDRILAAMKRGYTIIEYKSIIRKLRTIRPNI
CHHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHHHHHHCCCEEEHHHHHHHHHHHCCCCC
SISSDFIVGFPGETDTDFEETMKLIDDVKFDESFSFIYSPRPGTPASDLPDDTPYRIKLA
EECCCEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCCCCCCCCEEHHH
RLHQLQEKIQRNAQMISQSMVDTIQRVLVEGPSKKDPNEFCGRTDNNRVVNFAGHAGLVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCEEEECCCCCCEE
SFVDIKITAVSSHTLRGEISDMQ
CEEEEEEEEEECCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA